scholarly journals XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Lu Liu ◽  
Zhengjun Peng ◽  
Zhezhen Xu ◽  
Xi Wei
Keyword(s):  
Western Blot ◽  
Real Time ◽  
Real Time Pcr ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Multilineage Differentiation ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Introduction. Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown. Methods. To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot. DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.

Expression of MMP-13 (collagenase-3) in long-term cultures of human dental pulp cells

2008 ◽  
Vol 53 (8) ◽  
pp. 791-799 ◽  
Author(s):  
Lokesh Suri ◽  
Petros D. Damoulis ◽  
Trang Le ◽  
Eleni Gagari
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

scholarly journals OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Lu Liu ◽  
Rong Huang ◽  
Ruiqi Yang ◽  
Xi Wei
Keyword(s):  
In Situ Hybridization ◽  
Survival Rate ◽  
Western Blot ◽  
Real Time ◽  
Real Time Pcr ◽  
Dental Pulp ◽  
Immunofluorescence Staining ◽  
Dental Pulp Cells ◽  
Pulp Cells

Introduction. Infection and apoptosis are combined triggers for inflammation in dental tissues. Octamer-binding transcription factor 4-B1 (OCT4B1), a novel spliced variant of OCT4 family, could respond to the cellular stress and possess antiapoptotic property. However, its specific role in dental pulpitis remains unknown. Methods. To investigate the effect of OCT4B1 on inflammation of dental pulp cells (DPCs), its expression in inflamed dental pulp tissues and DPCs was examined by in situ hybridization, real-time PCR, and FISH assay. OCT4B1 overexpressed DPCs model was established, confirmed by western blot and immunofluorescence staining, and then stimulated with Lipopolysaccharide (LPS). Apoptotic rate was determined by Hoechst/PI staining and FACS. Cell survival rate was calculated by CCK8 assay. Results. In situ hybridization, real-time PCR, and FISH assay revealed that OCT4B1 was extensively expressed in inflamed dental pulp tissues and DPCs with LPS stimulation. Western blot and immunofluorescence staining showed the expression of OCT4B1 and OCT4B increased after OCT4B1 transfection. Hoechst/PI staining and FACS demonstrated that less red/blue fluorescence was detected and apoptotic percentage decreased (3.45%) after transfection. CCK8 demonstrated that the survival rate of pCDH-OCT4B1-flag cells increased. Conclusions. OCT4B1 plays an essential role in inflammation and apoptosis of DPCs. OCT4B might operate synergistically with OCT4B1 to reduce apoptosis.

The Recovery Effect of the Translationally Controlled Tumor Protein in 2-Hydroxy-Ethyl Methacrylate-Treated Human Dental Pulp Cells

2013 ◽  
Vol 747 ◽  
pp. 153-156
Author(s):  
Anchana Kongsaengkaeo ◽  
Wilaiwan Chotigeat ◽  
Ureporn Kedjarune-Leggat
Keyword(s):  
Real Time ◽  
Dental Pulp ◽  
Impedance Analysis ◽  
Dental Pulp Cells ◽  
Recovery Effect ◽  
Human Dental Pulp Cells ◽  
Translationally Controlled Tumor Protein ◽  
Ethyl Methacrylate ◽  
Human Dental Pulp ◽  
Pulp Cells

2-Hydroxy-ethyl methacrylate (HEMA) is a major monomer released from resin-base dental restorative materials. This study examined the recovery effect of the Translationally Controlled Tumor Protein (TCTP) in human dental pulp cells (HDPCs) after exposed to HEMA. TCTP from banana prawn (Penaeus merguiensis) was cloned and the protein was purified. A real-time cell analyzer was used to evaluate cell survival. The cell suspensions were seeded into an E-plate 96 at 8,000 cells/ well. After 24 hours, HDPCs were treated with 8 mM HEMA mixed in culture medium (alpha modified Eagles medium, α-MEM) for 1 hour before the medium supplemented with TCTP at 0, 100 ng/ml, 1 μg/ml, 10 μg/ml was replaced and left for 23 hours. After that, cells were fed with fresh medium for 72 hours. The cell indexes were monitored every 15 minutes and the 1-way analysis of variance (ANOVA) was used for statistical analysis. Real-time xCELLigence impedance analysis indicated that the cell indexes reached to 0 after treated with HEMA for 1 hour. TCTP at 1 μg/ml was significantly (P < 0.05) increased the cell index of HEME-treated HDPCs from 0 to 0.02 and 0.04 after TCTP exposure for 1 hour and 23 hours, respectively. It was suggested that TCTP has an ability to recover the cytotoxic effect of HEMA-treated pulp cells.

scholarly journals The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2449
Author(s):  
Beata Kaczmarek-Szczepańska ◽  
Olha Mazur ◽  
Marta Michalska-Sionkowska ◽  
Krzysztof Łukowicz ◽  
Anna Maria Osyczka
Keyword(s):  
Thermal Stability ◽  
Tannic Acid ◽  
Dental Pulp ◽  
Blood Compatibility ◽  
Dental Pulp Cells ◽  
Preliminary Assessment ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

Kallikrein Promotes Inflammation in Human Dental Pulp Cells Via Protease-Activated Receptor-1

2016 ◽  
Vol 117 (7) ◽  
pp. 1522-1528 ◽  
Author(s):  
Tomomi Hayama ◽  
Naoto Kamio ◽  
Tatsu Okabe ◽  
Koichiro Muromachi ◽  
Kiyoshi Matsushima
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Protease Activated Receptor 1

Effect of Nifedipine on the Differentiation of Human Dental Pulp Cells Cultured with Mineral Trioxide Aggregate

2013 ◽  
Vol 39 (6) ◽  
pp. 801-805 ◽  
Author(s):  
Su-Mi Woo ◽  
Yun-Chan Hwang ◽  
Hoi-Soon Lim ◽  
Nam-Ki Choi ◽  
Sun-Hun Kim ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Mineral Trioxide Aggregate ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Cells Cultured
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