scholarly journals Detection of Mercury Ion with High Sensitivity and Selectivity Using a DNA/Graphene Oxide Hybrid Immobilized on Glass Slides

Biosensors ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 300
Author(s):  
Li Gao ◽  
Qiuxiang Lv ◽  
Ni Xia ◽  
Yuanwei Lin ◽  
Feng Lin ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
High Selectivity ◽  
Limit Of Detection ◽  
Low Cost ◽  
High Sensitivity ◽  
Mercury Ions ◽  
Research Areas ◽  
Glass Slides ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity

Excessive mercury ions (Hg2+) cause great pollution to soil/water and pose a major threat to human health. The high sensitivity and high selectivity in the Hg2+ detection demonstrated herein are significant for the research areas of analytical chemistry, chemical biology, physical chemistry, drug discovery, and clinical diagnosis. In this study, a series of simple, low-cost, and highly sensitive biochips based on a graphene oxide (GO)/DNA hybrid was developed. Hg2+ is detected with high sensitivity and selectivity by GO/DNA hybrid biochips immobilized on glass slides. The performance of the biosensors can be improved by introducing more phosphorothioate sites and complementary bases. The best limit of detection of the biochips is 0.38 nM with selectivity of over 10:1. This sensor was also used for Hg2+ detection in Dendrobium. The results show this biochip is promising for Hg2+ detection.

Modern nanobiotechnologies for efficient detection and remediation of mercury

Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mulayam Singh Gaur ◽  
Rajni Yadav ◽  
Mamta Kushwah ◽  
Anna Nikolaevna Berlina
Keyword(s):  
Heavy Metals ◽  
Water Samples ◽  
Low Cost ◽  
High Sensitivity ◽  
Environmental Water ◽  
Mercury Ions ◽  
Content Type ◽  
Efficient Detection ◽  
Sensitivity And Selectivity ◽  
Selection Of

Purpose This information will be useful in the selection of materials and technology for the detection and removal of mercury ions at a low cost and with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. Design/methodology/approach Different nano- and bio-materials allowed for the development of a variety of biosensors – colorimetric, chemiluminescent, electrochemical, whole-cell and aptasensors – are described. The materials used for their development also make it possible to use them in removing heavy metals, which are toxic contaminants, from environmental water samples. Findings This review focuses on different technologies, tools and materials for mercury (heavy metals) detection and remediation to environmental samples. Originality/value This review gives up-to-date and systemic information on modern nanotechnology methods for heavy metal detection. Different recognition molecules and nanomaterials have been discussed for remediation to water samples. The present review may provide valuable information to researchers regarding novel mercury ions detection sensors and encourage them for further research/development.

scholarly journals Smartphone-Based Microfluidic Colorimetric Sensor for Gaseous Formaldehyde Determination with High Sensitivity and Selectivity

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 3141 ◽  
Author(s):  
Xiao-Liang Guo ◽  
Yan Chen ◽  
Hong-Lan Jiang ◽  
Xian-Bo Qiu ◽  
Du-Li Yu
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Low Cost ◽  
Sick Building Syndrome ◽  
High Sensitivity ◽  
Colorimetric Sensor ◽  
World Health ◽  
Formaldehyde Determination ◽  
Sensitivity And Selectivity ◽  
Gaseous Formaldehyde

Formaldehyde is one of the most dangerous air pollutants, which can cause sick building syndrome. Thus, it is very crucial to precisely determine formaldehyde with a low cost and simple operation. In this paper, a smartphone-based microfluidic colorimetric sensor is devised for gaseous formaldehyde determination with high sensitivity and selectivity. Specifically, a novel microfluidic chip is proposed based on the 4-aminohydrazine-5-mercapto-1,2,4-triazole (AHMT) method to determine formaldehyde; the chip consists of two reagent reservoirs, one reaction reservoir and a mixing column. In this design to prevent the fluid from flowing out while letting the gas molecule in, a hydrophobic porous poly tetra fluoroethylene (PTFE) membrane is put on the top of the reaction reservoir. Using the microfluidic chip sensor, a smartphone-based formaldehyde determination system is developed, which makes the measuring process automated and simple. As per the experiment results, the limit-of-detection (LOD) of the system is as low as 0.01 ppm, which is much lower than the maximum exposure concentration (0.08 ppm) recommended by the World Health Organization (WHO). Moreover, the sensor is hardly affected by acetaldehyde, volatile organic compounds (VOCs) or acidic-alkaline, which shows great selectivity. Finally, the performance of the proposed sensor is verified by using it for the determination of formaldehyde in a newly decorated house.

scholarly journals Mono-6-Deoxy-6-Aminopropylamino-β-Cyclodextrin on Ag-Embedded SiO2 Nanoparticle as a Selectively Capturing Ligand to Flavonoids

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1349 ◽  
Author(s):  
Eunil Hahm ◽  
Eun Ji Kang ◽  
Xuan-Hung Pham ◽  
Daham Jeong ◽  
Dae Hong Jeong ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Raman Intensity ◽  
Trace Quantity ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
Similar Materials ◽  
The Relationship ◽  
Selective Technique

It has been increasingly important to develop a highly sensitive and selective technique that is easy to handle in detecting levels of beneficial or hazardous analytes in trace quantity. In this study, mono-6-deoxy-6-aminopropylamino-β-cyclodextrin (pr-β-CD)-functionalized silver-assembled silica nanoparticles (SiO2@Ag@pr-β-CD) for flavonoid detection were successfully prepared. The presence of pr-β-CD on the surface of SiO2@Ag enhanced the selectivity in capturing quercetin and myricetin among other similar materials (naringenin and apigenin). In addition, SiO2@Ag@pr-β-CD was able to detect quercetin corresponding to a limit of detection (LOD) as low as 0.55 ppm. The relationship between the Raman intensity of SiO2@Ag@pr-β-CD and the logarithm of the Que concentration obeyed linearity in the range 3.4–33.8 ppm (R2 = 0.997). The results indicate that SiO2@Ag@pr-β-CD is a promising material for immediately analyzing samples that demand high sensitivity and selectivity of detection.

scholarly journals Highly Sensitive Electrochemical Aptasensor for Detecting the VEGF165 Tumor Marker with PANI/CNT Nanocomposites

Biosensors ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 114
Author(s):  
Yunjeong Park ◽  
Min-Sung Hong ◽  
Woo-Hyuk Lee ◽  
Jung-Gu Kim ◽  
Kyunghoon Kim
Keyword(s):  
Tumor Marker ◽  
Limit Of Detection ◽  
Biological Fluid ◽  
Low Cost ◽  
High Sensitivity ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Promising Tool ◽  
Highly Sensitive ◽  
Endothelial Growth Factor

Sensing targeted tumor markers with high sensitivity provides vital information for the fast diagnosis and treatment of cancer patients. A vascular endothelial growth factor (VEGF165) have recently emerged as a promising biomarker of tumor cells. The electrochemical aptasensor is a promising tool for detecting VEGF165 because of its advantages such as a low cost and quantitative analysis. To produce a sensitive and stable sensor electrode, nanocomposites based on polyaniline (PANI) and carbon nanotube (CNT) have potential, as they provide for easy fabrication, simple synthesis, have a large surface area, and are suitable in biological environments. Here, a label-free electrochemical aptasensor based on nanocomposites of CNT and PANI was prepared for detecting VEGF165 as a tumor marker. The nanocomposite was assembled with immobilized VEGF165 aptamer as a highly sensitive VEGF165 sensor. It exhibited stable and wide linear detection ranges from 0.5 pg/mL to 1 μg/mL, with a limit of detection of 0.4 pg/mL because of the complementary effect of PANI/CNT. The fabricated aptasensor also exhibited good stability in biological conditions, selectivity, and reproducibility after several measurement times after the dissociation process. Thus, it could be applied for the non-invasive determination of VEGF, in biological fluid diagnosis kits, or in an aptamer-based biosensor platform in the near future.

scholarly journals Highly Sensitive Detection for Mercury Ions Using Graphene Oxide (GO) Sensors

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1070
Author(s):  
Lei Liu ◽  
Haixia Shi ◽  
Raoqi Li ◽  
Cheng Liu ◽  
Jia Cheng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescent Dyes ◽  
Limit Of Detection ◽  
Sybr Green ◽  
Sybr Green I ◽  
Mercury Ions ◽  
Amino Groups ◽  
Highly Sensitive ◽  
Detection Of Signals ◽  
Highly Sensitive Detection

The mercury ion (Hg2+) is one of the heavy metal ions, and its presence in trace amounts can cause physiological damage to an organism. Traditional methods of Hg2+ detection have been useful but have also had numerous limitations and challenges, and as a result, it is important to design new and sophisticated methods that can aid in the detection of Hg2+. In this paper, two fluorescent dyes, carboxyfluorescein (FAM) and SYBR Green I, were used to label and intercalate DNA probes immobilized on the surface of graphene oxide (GO) for sensors to detect Hg2+. FAM and SYBR Green I dye share close excitation and emission wavelength spectra, which can promote and amplify the detection of signals, and also increase the limit of detection (LOD). The results showed that the limit of detection in this method was 0.53 nM. Moreover, when the sensors with double amino groups on the surface of GO were carried out to detect Hg2+, a limit of detection was improved to 0.43 nM. The sensors were then applied in the real sample. The results show that this method has a promising potential in Hg2+ detection.

scholarly journals An Enzyme-Based Biosensor for the Detection of Organophosphate Compounds Using Mutant Phosphotriesterase Immobilized onto Reduced Graphene Oxide

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Muhammad Syafiq Mohd Razib ◽  
Wahhida Latip ◽  
Jahwarhar Izuan Abdul Rashid ◽  
Victor Feizal Knight ◽  
Wan Md Zin Wan Yunus ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
High Selectivity ◽  
Immobilized Enzyme ◽  
Limit Of Detection ◽  
Electrochemical Analysis ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Enzymatic Detection

Enzymatic detection of organophosphate (OP) compounds can be tailored using highly sensitive and selective enzymes in the development of biosensors. Previously, mutant (YT) phosphotriesterase (PTE) was reported to efficiently hydrolyze Sp and Rp enantiomers of phosphotriester. This study reports the use of phosphotriesterase mutant YT (YT-PTE) immobilized onto reduced graphene oxide (rGO) and fabricated onto a screen-printed carbon electrode (SPCE) for electrochemical detection of OP compounds. Immobilization of YT-PTE onto rGO was secured using N-hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) cross-linker, and the resulting immobilized enzyme was able to retain up to 90% of its activity. Electrochemical analysis of the SPCE/rGO/YT-PTE showed detection of paraoxon in a linear range of 1 mM–0.005 μM with its limit of detection as low as 0.11 μM. SPCE/rGO/YT-PTE exhibited high selectivity towards paraoxon and parathion and have good reproducibility. Furthermore, detection of paraoxon was also possible in a real water sample with only minor interferences.

scholarly journals An antibody-graphene oxide nanoribbon conjugate as a surface enhanced laser desorption/ionization probe with high sensitivity and selectivity

2015 ◽  
Vol 51 (22) ◽  
pp. 4619-4622 ◽  
Author(s):  
Jing Wang ◽  
Mengting Cheng ◽  
Zhen Zhang ◽  
Liangqia Guo ◽  
Qian Liu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
High Sensitivity ◽  
Laser Desorption Ionization ◽  
Functionalized Graphene ◽  
Mass Spectrometry Detection ◽  
Functionalized Graphene Oxide ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Sensitivity And Selectivity ◽  
Ionization Probe

Antibody-functionalized graphene oxide nanoribbons were synthesized and applied as highly sensitive and selective SELDI probes for mass spectrometry detection.

A novel aptasensor based on single-molecule force spectroscopy for highly sensitive detection of mercury ions

The Analyst ◽  
2015 ◽  
Vol 140 (15) ◽  
pp. 5243-5250 ◽  
Author(s):  
Qing Li ◽  
Monika Michaelis ◽  
Gang Wei ◽  
Lucio Colombi Ciacchi
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Mercury Ions ◽  
Single Molecule Force Spectroscopy ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
Highly Sensitive Detection

A novel aptasensor based on single-molecule force spectroscopy shows high sensitivity and selectivity for sensing mercury ions.

scholarly journals A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Guanhua Xun ◽  
Stephan Thomas Lane ◽  
Vassily Andrew Petrov ◽  
Brandon Elliott Pepa ◽  
Huimin Zhao
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
High Volume ◽  
N Gene ◽  
Testing System ◽  
Target Sequence ◽  
One Pot ◽  
Sequence Detection ◽  
Highly Sensitive ◽  
Speed Accuracy

AbstractThe need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.

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