scholarly journals One-step Synthesis of Silver Nanoparticles Using Saudi Arabian Desert Seasonal Plant Sisymbrium irio and Antibacterial Activity Against Multidrug-Resistant Bacterial Strains

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 662 ◽  
Author(s):  
Mickymaray
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Multidrug Resistant ◽  
Desert Plant ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Acinetobacter Baumanii ◽  
Tem Analysis ◽  
One Step ◽  
Sisymbrium Irio

Globally, antimicrobial resistance has grown at an alarming rate. To combat the multidrug-resistant (MDR) superbugs, silver nanoparticles (Ag NPs) were synthesized using an aqueous leaf extract of seasonal desert plant Sisymbrium irio obtained from the central region of Saudi Arabia by a simple one-step procedure. The physical and chemical properties of the Ag NPs were investigated through ultraviolet visisble analysis (UV-vis), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM) analysis. The UV-vis spectrum showed an absorption band at 426 nm. The XRD results showed a highly crystalline face-centered cubic structure. The surface morphology analyzed using SEM and TEM analyses showed the particle size to be in the range 24 nm to 50 nm. Various concentrations of Ag NPs were tested against MDR Pseudomonas aeruginosa and Acinetobacter baumanii that cause ventilator-associated pneumonia (VAP). American Type Culture Collection (ATCC) Escherichia coli-25922 was used as the reference control strain. The Ag NPs effectively inhibited tested pathogens, even at the lowest concentration (6.25 µg) used. The bacterial inhibitory zone ranged from 11–21 mm. In conclusion, the newly synthesized Ag NPs could be a potential alternative candidate in biomedical applications in controlling the spread of MDR pathogens.

scholarly journals Biosynthesis and Characterization of Silver Nanoparticles produced by Phormidium ambiguum and Desertifilum tharense

2021 ◽  
Author(s):  
Amira Lotfy Hanna ◽  
Hayam Hamouda ◽  
Hanan Goda ◽  
Tarek Elsayed ◽  
Mahmoud Sadik
Keyword(s):  
Silver Nanoparticles ◽  
Micrococcus Luteus ◽  
16S Rrna Gene Sequencing ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Rrna Gene Sequencing

Abstract The world faces a challenge with pervasion of multidrug resistant bacteria which encouraged the scientists to develop and discover alternative ecofriendly and easy to produce new antibacterial agents. Two Egyptian cyanobacteria were isolated and identified according to 16S rRNA gene sequencing as Phormidium ambiguum and Desertifilum tharense . The sequences were deposited in the GenBank with accession numbers of MW762709 and MW762710 for Desertifilum tharense and Phormidium ambiguum, respectively. These isolates have the ability to produce silver nanoparticles (Ag-NPs) extra- and intracellularly under light and dark conditions. The results of UV-Vis analysis showed promising extracellular Ag-NPs synthesis by Desertifilum tharense and Phormidium ambiguum under light conditions. Therefore, these Ag-NPs were characterized and evaluated for antibacterial and antioxidant activity. TEM, SEM and XRD analyses revealed the spherical crystals with face-centered cubic structures and size range of 6.24–11.4 nm and 6.46–12.2 nm for Ag-NPs of Desertifilum tharense and Phormidium ambiguum , respectively. XRD and EDX results clearly confirmed the successful synthesis of Ag-NPs in its oxide form or chloride form. The FTIR spectrum data confirmed the presence of hydroxyl and amide groups. Desertifilum tharense Ag-NPs displayed the largest inhibition zone ranged from 9 mm against Micrococcus luteus ATCC 10240 to 25 mm against methicillin resistant S. aureus (MRSA) ATCC 43300. For Phormidium ambiguum Ag-NPs, the inhibition zone diameter was in a range of 9–18 mm. The Ag-NPs of Phormidium ambiguum exhibited the highest scavenging activity of 48.7% comparing with that of Desertifilum tharense which displayed 43.753%.

scholarly journals Colorimetric Detection of Ammonia Using Synthesized Silver Nanoparticles from Durian Fruit Shell

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Eman Alzahrani
Keyword(s):  
Silver Nanoparticles ◽  
Optical Measurement ◽  
Biological Fluid ◽  
Colorimetric Detection ◽  
Ammonia Concentration ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Tem Analysis ◽  
Ammonia Sensing ◽  
Absorption Ratio

There has been increased interest in the production of nanoparticles (NP) through green chemistry. This article used durian fruit shell aqueous solution that acts as a reductive preparation of silver NPs. The silver nanoparticles have a size of approximately 25 nm. The NP size uniformity was determined by the SEM and TEM analysis. X-ray diffraction technique was used to characterize crystalline silver nanoparticles face-centered cubic structure. XPS spectrum showed distinct silver peaks on the nanoparticles’ surface. An optical method that was based on surface plasmon resonance (SPR) was used to perform the green Ag NPs aqueous ammonia sensing study. Optical measurement facilitated the ammonia sensing study of Ag NPs that had been prepared. The study also investigated the performance of the optical sensor, thus adding validity to the study. Also, the research sought to determine how the concentration of ammonia in ammonia sensing affects the Ag NPs that had been obtained. The study observed a linear relationship with R2 as the correlation factor which was equal to 0.9831. This was observed from the ammonia concentration plot versus absorption ratio that suggested that there was a linear increase in absorption ratio with increase in ammonia concentration. The study significance is that the room temperature optical ammonia sensor can be used in future for medical diagnosis in the detection of low levels of ammonia in biological fluid like sweat, cerebrospinal fluid, saliva, plasma, or biological samples. This enhances the application of the technique in human biomedical applications.

scholarly journals Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 473
Author(s):  
Prabu Kumar Seetharaman ◽  
Rajkuberan Chandrasekaran ◽  
Rajiv Periakaruppan ◽  
Sathishkumar Gnanasekar ◽  
Sivaramakrishnan Sivaperumal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Culture Filtrate ◽  
Morphological Changes ◽  
Xrd Analysis ◽  
Penicillium Oxalicum ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
X Ray ◽  
Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

scholarly journals Biogenic silver nanoparticles from Trichodesma indicum aqueous leaf extract against Mythimna separata and evaluation of its larvicidal efficacy

2017 ◽  
Vol 57 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Abdul A. Buhroo ◽  
Gousul Nisa ◽  
Syed Asrafuzzaman ◽  
Ram Prasad ◽  
Razia Rasheed ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Average Particle ◽  
Particle Sizes ◽  
Mythimna Separata ◽  
Ag Nps ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
Vis Spectroscopy ◽  
Aqueous Leaf Extract

AbstractThe present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) usingTrichodesma indicumaqueous leaf extract as a reducing agent. The synthesized Ag NPs were productively characterized by UV-vis spectroscopy, XRD, and TEM studies. The photosynthesis of Ag NPs was done at room temperature for 24 h and at 60°C. The green synthesis of spherical-shaped Ag NPs bio-fabricated fromT. indicumwith a face centred cubic structure showed average particle sizes of 20–50 nm, which is inconsistent with the particle size calculated by the XRD Scherer equation and TEM analysis. We further explored the larvicidal efficacy of biosynthesized Ag NPs with leaf extracts ofT. indicumagainstMythimna separata. The results showed that Ag NPs (20–50 nm) ofT. indicumpossess good larvicidal activity againstM. separatawith an LC50of 500 ppm. Thus, we can advocate that Ag NPs of 20–50 nm size extracted fromT. indicummay be considered in the pest management programme ofM. separatain future.

scholarly journals Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sudip Some ◽  
Onur Bulut ◽  
Kinkar Biswas ◽  
Anoop Kumar ◽  
Anupam Roy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bombyx Mori ◽  
Leaf Extract ◽  
Silver Ions ◽  
Average Diameter ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
Beneficial Effects ◽  
Selected Area Electron Diffraction ◽  
Face Centered

Abstract Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

scholarly journals Green Synthesis of Silver Nanoparticles in the Presence of Polysaccharide: Optimization and Characterization

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Margarita I. Skiba ◽  
Victoria I. Vorobyova ◽  
Alexander Pivovarov ◽  
Natalya P. Makarshenko
Keyword(s):  
Silver Nanoparticles ◽  
Sodium Alginate ◽  
Xrd Analysis ◽  
Face Centered Cubic ◽  
Colloidal Solutions ◽  
Ag Nps ◽  
Sem Images ◽  
Spherical Powder ◽  
Stabilizing Agent ◽  
Vis Spectroscopy

The process of obtaining aqueous solutions of silver nanoparticles with the use of a low-temperature nonequilibrium contact plasma and stabilizing agent—polysaccharide (sodium alginate)—has been examined. The synthesized Ag NPs were characterized by using UV-Vis spectroscopy, dynamic light scattering (DLS), scanning electron microscope (SEM), and XRD analysis. The effect of concentration of Ag+, sodium alginate, duration of processing by plasma discharge, and pH of liquid on the production of silver nanoparticles has been studied. The results demonstrated that synthesis provides the formation of silver nanoparticles for investigated concentrations of Ag+ (0.3-3.0 mmol/l) and 5.0 g/l Na-Alg (pH=7–10) within 1–5 minutes. From the SEM images, the silver nanoparticles are found to be almost spherical. Powder XRD results reveal that Ag nanoparticles have a face-centered cubic crystal structure. Zeta potential of plasma-chemically obtained colloidal solutions at various concentrations of Ag+ ions and stabilizing agent varies from −32.8 to −39.3 mV, indicating the moderate stability of synthesized nanoparticles.

scholarly journals Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ragaa A. Hamouda ◽  
Mervat H. Hussein ◽  
Rasha A. Abo-elmagd ◽  
Salwa S. Bawazir
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
Fourier Transforms ◽  
Human Colon ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Ag Nps ◽  
Human Colon Cancer ◽  
Visible Absorption ◽  
Ft Ir

Abstract Using aqueous cyanobacterial extracts in the synthesis of silver nanoparticle is looked as green, ecofriendly, low priced biotechnology that gives advancement over both chemical and physical methods. In the current study, an aqueous extract of Oscillatoria limnetica fresh biomass was used for the green synthesis of Ag-NPs, since O. limnetica extract plays a dual part in both reducing and stabilizing Oscillatoria-silver nanoparticles (O-AgNPs). The UV-Visible absorption spectrum, Fourier transforms infrared (FT-IR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) were achieved for confirming and characterizing the biosynthesized O-AgNPs. TEM images detected the quasi-spherical Ag-NPs shape with diverse size ranged within 3.30–17.97 nm. FT-IR analysis demonstrated the presence of free amino groups in addition to sulfur containing amino acid derivatives acting as stabilizing agents as well as the presence of either sulfur or phosphorus functional groups which possibly attaches silver. In this study, synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast (MCF-7) cell line giving IC50 (6.147 µg/ml) and human colon cancer (HCT-116) cell line giving IC50 (5.369 µg/ml). Hemolytic activity of Ag-NPs was investigated and confirmed as being non- toxic to human RBCs in low concentrations.

scholarly journals Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain ofPseudomonas aeruginosaand Activity as Broad Spectrum Clinical Antibacterial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Melisa A. Quinteros ◽  
Ivana M. Aiassa Martínez ◽  
Pablo R. Dalmasso ◽  
Paulina L. Páez
Keyword(s):  
Silver Nanoparticles ◽  
Broad Spectrum ◽  
Nitrate Solution ◽  
Human Neutrophils ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Ag Nps ◽  
Tem Analysis ◽  
Vis Spectroscopy

Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobial properties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using aPseudomonas aeruginosastrain from a reference culture collection. A greenish culture supernatant ofP. aeruginosaincubated at 37°C with a silver nitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UV-vis spectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudospherical nanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of Ag-NPs. Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic and opportunistic microorganisms, namely,Staphylococcus aureus,Staphylococcus epidermidis,Enterococcus faecalis,Proteus mirabilis,Acinetobacter baumannii,Escherichia coli,P. aeruginosa, andKlebsiella pneumonia. Ag-NPs were found to be bioactive at picomolar concentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This work demonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenic multidrug-resistant bacteria such as methicillin-resistantS. aureus,A. baumannii, andE. coli. In addition, these Ag-NPs showed negligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.

One-step hydrothermal synthesis of silver nanoparticles loaded on N-doped carbon and application for catalytic reduction of 4-nitrophenol

RSC Advances ◽  
2015 ◽  
Vol 5 (106) ◽  
pp. 87151-87156 ◽  
Author(s):  
Yuhan Wu ◽  
Zhiqiang Wang ◽  
Shanshan Chen ◽  
Jianning Wu ◽  
Xuhong Guo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Hydrothermal Synthesis ◽  
Catalytic Reduction ◽  
Ag Nps ◽  
One Step

In this work, we report a novel and facile one-step approach for synthesis of silver nanoparticles (Ag NPs) loaded on N-doped carbon (NC) composites.

scholarly journals Radiation Induced Silver Nanoparticles in Ionically Crosslinked Alginate Beads for Efficient Catalytic Degradation of Basic Dye

2021 ◽  
Author(s):  
Manal F. abou Taleb ◽  
Faten Ismail Abou El Fadl ◽  
Hanan Albalwi ◽  
Mohamed M. Ibrahim
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Calcium Alginate ◽  
Degradation Kinetics ◽  
Visible Spectrum ◽  
Catalytic Degradation ◽  
Second Order ◽  
Blue Dye ◽  
Ag Nps ◽  
Pseudo Second Order

Abstract Silver nanoparticles (Ag NPs) are widely used as engineered nanomaterials in many advanced nanotechnologies, due to their versatile, easy and cheap preparations combined with peculiar chemical-physical properties. Their increased production and integration in environmental applications including water treatment raise concerns for their impact on humans and the environment. In this study, gamma radiation dose of 20 kGy was utilized to induce the synthesis silver nanoparticles (Ag NPs) in the alginate micro beads to prepare calcium Alginate/Ag (Ca-ALG/Ag) nanocomposite beads. These beads were then used to degrade toxic basic dyes in waste water. Initially, Ca-ALG /Ag nanocomposite beads were synthesized and characterized using Ultra Violet-visible spectrum (UV-Vis), Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) and Transmission Electron Microscope (TEM) for confirming the formation of Ag NPs and morphological study. The catalytic efficiency of the resulting Ca-ALG/Ag nanocomposite beads is evaluated for the degradation of dyes such sandocryl blue dye in the presence of NaBH4. The catalytic degradation of sandocryl blue dye was very fast in the present conditions: 0.1 g of catalyst 5 ml NaBH4 and the optimum time for complete reduction was 30 min. The pseudo-first order, pseudo second order and intra particle diffusion model used to evaluate the kinetic models and the mechanism of the degradation. Results showed that the degradation kinetics best fit the pseudo-second-order model and Langmuir isotherm model. The Biopolymer-based nanocomposite beads of calcium alginate, and Ag NPs can be applied to reduce dyestuff, where it is economically viable and environmentally friendly.

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