scholarly journals Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain ofPseudomonas aeruginosaand Activity as Broad Spectrum Clinical Antibacterial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Melisa A. Quinteros ◽  
Ivana M. Aiassa Martínez ◽  
Pablo R. Dalmasso ◽  
Paulina L. Páez
Keyword(s):  
Silver Nanoparticles ◽  
Broad Spectrum ◽  
Nitrate Solution ◽  
Human Neutrophils ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Ag Nps ◽  
Tem Analysis ◽  
Vis Spectroscopy

Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobial properties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using aPseudomonas aeruginosastrain from a reference culture collection. A greenish culture supernatant ofP. aeruginosaincubated at 37°C with a silver nitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UV-vis spectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudospherical nanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of Ag-NPs. Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic and opportunistic microorganisms, namely,Staphylococcus aureus,Staphylococcus epidermidis,Enterococcus faecalis,Proteus mirabilis,Acinetobacter baumannii,Escherichia coli,P. aeruginosa, andKlebsiella pneumonia. Ag-NPs were found to be bioactive at picomolar concentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This work demonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenic multidrug-resistant bacteria such as methicillin-resistantS. aureus,A. baumannii, andE. coli. In addition, these Ag-NPs showed negligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.

scholarly journals Biogenic silver nanoparticles from Trichodesma indicum aqueous leaf extract against Mythimna separata and evaluation of its larvicidal efficacy

2017 ◽  
Vol 57 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Abdul A. Buhroo ◽  
Gousul Nisa ◽  
Syed Asrafuzzaman ◽  
Ram Prasad ◽  
Razia Rasheed ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Average Particle ◽  
Particle Sizes ◽  
Mythimna Separata ◽  
Ag Nps ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
Vis Spectroscopy ◽  
Aqueous Leaf Extract

AbstractThe present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) usingTrichodesma indicumaqueous leaf extract as a reducing agent. The synthesized Ag NPs were productively characterized by UV-vis spectroscopy, XRD, and TEM studies. The photosynthesis of Ag NPs was done at room temperature for 24 h and at 60°C. The green synthesis of spherical-shaped Ag NPs bio-fabricated fromT. indicumwith a face centred cubic structure showed average particle sizes of 20–50 nm, which is inconsistent with the particle size calculated by the XRD Scherer equation and TEM analysis. We further explored the larvicidal efficacy of biosynthesized Ag NPs with leaf extracts ofT. indicumagainstMythimna separata. The results showed that Ag NPs (20–50 nm) ofT. indicumpossess good larvicidal activity againstM. separatawith an LC50of 500 ppm. Thus, we can advocate that Ag NPs of 20–50 nm size extracted fromT. indicummay be considered in the pest management programme ofM. separatain future.

scholarly journals Green Synthesis of Silver Nanoparticles and Evaluation of Their Antibacterial Activity against Multidrug-Resistant Bacteria and Wound Healing Efficacy Using a Murine Model

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 902
Author(s):  
Vajravathi Lakkim ◽  
Madhava C. Reddy ◽  
Roja Rani Pallavali ◽  
Kakarla Raghava Reddy ◽  
Ch Venkata Reddy ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Multidrug Resistant ◽  
Biomedical Science ◽  
Resistant Bacteria ◽  
Zone Of Inhibition ◽  
Ag Nps ◽  
Bacteriolytic Activity ◽  
Vis Spectroscopy

Green nanotechnology has significant applications in various biomedical science fields. In this study, green-synthesized silver nanoparticles, prepared by using Catharanthus roseus and Azadirachta indica extracts, were characterized using UV–Vis spectroscopy, dynamic light scattering, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Silver nanoparticles (Ag NPs) synthesized from leaf extracts of C. roseus and A. indica effectively inhibited the growth of multidrug-resistant (MDR) bacteria isolated from patients with septic wound infections. The maximum bacteriolytic activity of the green-synthesized Ag NPs of C. roseus and A. indica against the MDR bacterium K. Pneumoniae was shown by a zone of inhibition of 19 and 16 mm, respectively. C. roseus Ag NPs exhibited more bacteriolytic activity than A. indica Ag NPs in terms of the zone of inhibition. Moreover, these particles were effective in healing wounds in BALB/c mice. Ag NPs of C. roseus and A. indica enhanced wound healing by 94% ± 1% and 87% ± 1%, respectively. Our data suggest that Ag NPs from C. roseus and A. indicia ameliorate excision wounds, and wound healing could be due to their effective antimicrobial activity against MDR bacteria. Hence, these Ag NPs could be potential therapeutic agents for the treatment of wounds.

scholarly journals Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi

2021 ◽  
Vol 10 (1) ◽  
pp. 569-584
Author(s):  
Mehreen Zaka ◽  
Syed Salman Hashmi ◽  
Moiz A. Siddiqui ◽  
Lubna Rahman ◽  
Sadaf Mushtaq ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Cannabis Sativa ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Ag Nps ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Antibacterial And Antifungal Activities ◽  
Vis Spectroscopy ◽  
Bacteria And Fungi

Abstract In this paper, we have presented the method of green synthesis of ZnO and Ag-NPs using the callus extract (CE) of medicinally important Cannabis sativa. The synthesis of nanoparticles (NPs) was confirmed by UV-Vis spectroscopy, while as far as the size and shape of the NPs were concerned, they were validated using the techniques of X-ray diffraction and scanning electron microscopy, respectively. The energy dispersive X-ray analysis graph confirmed the constitution of elements along with the surface chemical state of NPs. Fourier transform-infrared spectroscopy was utilized for the confirmation of biomolecules capping the NPs. In order to test the application of these biosynthesized NPs on biological entities, four bacterial strains, including Bacillus subtilis, Klebsiella pneumonia, Staphylococcus aureus, and Pseudomonas aeruginosa, were used. On the other hand, five fungal strains, namely Mucor, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, and Fusarium solani, were utilized for antifungal assay. Cytotoxicity assay was also performed using the HepG2 cell line. The results showed considerable antibacterial and antifungal activities. It also showed better cytotoxicity values as compared to the control.

scholarly journals Green Synthesis of Silver Nanoparticles Using Hibiscus sabdariffa Leaves Extract

2021 ◽  
Vol 3 (1) ◽  
pp. 76-81
Author(s):  
Siti Husnaa Mohd Taib ◽  
Kamyar Shameli ◽  
Roshafima Rasit Ali ◽  
Zahra Izadiyan ◽  
Zatil Izzah Ahmad Tarmizi
Keyword(s):  
Silver Nanoparticles ◽  
Hibiscus Sabdariffa ◽  
Ag Nps ◽  
Tem Analysis ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Size Of Particles ◽  
Average Size

The present paper reports the synthesis of silver nanoparticles (Ag-NPs) by a green method using Hibiscus sabdariffa (H. sabdariffa) leaves extract as reductant and stabilizer. The synthesized Ag-NPs were characterized by ultraviolet-visible (UV-vis) spectroscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). UV-vis spectrum of synthesized Ag-NPs showed a peak at 378 nm. TEM analysis revealed that the particles were spherical and irregular in shape and has average size around 56.52 nm. This structure and size of particles were confirmed by AFM analysis. The UV-vis and FTIR spectrum provides evidence of the presence of caffeic acid component as a representative biomolecule in stabilising the nanoparticles based on previous studies. Hence, this study advocates that H. sabdariffa have potential for synthesizing nanoparticles.

Synthesis and Biological Characterization of Silver Nanoparticles Biosynthesized by Semenovia suffruticosa

2021 ◽  
Vol 66 ◽  
pp. 45-60
Author(s):  
Sara Soltanian ◽  
Sheikhbahaei Mahboubeh ◽  
Sharifi Fatemeh ◽  
Neda Mohamadi
Keyword(s):  
Silver Nanoparticles ◽  
Leishmania Major ◽  
Biological Activities ◽  
Annexin V ◽  
Spherical Shape ◽  
Cytotoxic Activities ◽  
Bacterial Strains ◽  
Ag Nps ◽  
Hek 293 ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) were synthesized using methanol extract of Semenovia. suffruticosa. The prepared AgNPs (SS-AgNPs) were examined by ultraviolet-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-raydiffraction (XRD), transmission electron microscopy (TEM) and scanning electron microscope (SEM). Afterward, biological activities including cytotoxicity, ability to generate reactive oxygen species (ROS), antileishmanial and antibacterial effects were investigated. According to the UV-Vis spectroscopy, absorption peak at 430 nm indicates the synthesis of AgNPs. TEM and SEM image of AgNPs shows spherical shape with size range of 20–70 nm. FTIR analysis displayed the involvement of phytochemical constituents in synthesized nanoparticles. The X-ray diffraction analysis confirmed the synthesis of highly pure AgNPs with high crystallinity and Cubic shape with crystalline size of 21.4 nm. SS-AgNPs were cytotoxic on cell lines with IC50 values of 15, 20, 20 and 26 µg/mL in HEK 293, Caco-2, SH-SY5Y and MDA-MD-231 cells, respectively. DCFH-DA assay showed that 24 h exposure to 25, 50, 100, 200 µg/mL concentrations of SS-AgNPs significantly increased production of ROS in cells that indicate oxidative stress induction by SS-AgNPs. Annexin V-PE/7-AAD staining analysis revealed a combination of apoptosis and necrosis following the exposure of Ag NPs to cells. SS-AgNPs displayed a notable bactericidal activity against Gram-negative bacterial strains. SS-AgNPs revealed remarkable antileishmanial activity against the promastigote and amastigote stages of Leishmania. major. IC50 values of SS-AgNPs were 16.17 and 6.35 using promastigote and amastigotes assay respectively. Conclusively, phytosynthesized AgNPs is effective in antileishmanial, antimicrobial and cytotoxic activities.

scholarly journals Potential antibacterial activity of crude extracts and silver nanoparticles synthesized from Sargassum wightii

2014 ◽  
Vol 3 (10) ◽  
pp. 322-325 ◽  
Author(s):  
Vinoth Kumar Thirumalairaj ◽  
Mahitha Puthanpurayal Vijayan ◽  
Geetharamani Durairaj ◽  
Lakshmanasenthil Shanmugaasokan ◽  
Rincy Yesudas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Zone Of Inhibition ◽  
Ag Nps ◽  
Sargassum Wightii ◽  
Solvent Extract ◽  
Vis Spectroscopy

The present work investigates the antibacterial activity of silver nanoparticles (Ag-NPs) synthesized by biological method using Sargassum wightii. The fresh live seaweed was collected from the Mandapam coast of Tamilnadu, India. Solvent extract was prepared using acetone, petroleum ether and methanol. Aqueous extract of the seaweed was also used for the synthesis of silver Ag-NPs. Seaweed extract is used as a reducing agent of 2mM silver nitrate solution for the synthesis of Ag-NPs. Periodical monitoring of reaction mixture was done using UV-vis spectroscopy at 300-750 nm. The scanning electron microscopy (SEM) of the sample confirms the presence of Ag-NPs. The antibacterial activity of solvent extract was done by Minimal inhibitory concentration (MIC) assay. The methanol extract of the seaweed at a concentration of 250µg/ml exhibited potent antimicrobial activity against the test microorganism. The zone of inhibition ranging from 8-14 mm was observed with different extracts. The antibacterial activity of the synthesized Ag-NPs against the organism was also done by MIC test. The MIC of Ag-NPs was found to be 130µg/ml for all pathogenic microorganisms selected for the study. The zone of inhibition against Bacillus cereus, Bacillus anhtracis, Staphylococcus aureus and Vibrio alginoyticus were found to be 10, 8, 10 and 9 mm, respectively. The synthesized Ag-NPs exhibited significant antimicrobial activity against the selected microorganisms than the solvent extract of seaweed.DOI: http://dx.doi.org/10.3329/icpj.v3i10.20337 International Current Pharmaceutical Journal, September 2014, 3(10): 322-325

scholarly journals Green synthesis of silver nanoparticles: Characterization and its potential biomedical applications

2021 ◽  
Vol 10 (1) ◽  
pp. 412-420
Author(s):  
Mona S. Alwhibi ◽  
Dina A. Soliman ◽  
Manal A. Awad ◽  
Asma B. Alangery ◽  
Horiah Al Dehaish ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aloe Vera ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Noble Metal Nanoparticles ◽  
Biological Synthesis ◽  
Ag Nps ◽  
Mouse Melanoma ◽  
Therapeutic Benefits ◽  
Vis Spectroscopy

Abstract In recent times, research on the synthesis of noble metal nanoparticles (NPs) has developed rapidly and attracted considerable attention. The use of plant extracts is the preferred mode for the biological synthesis of NPs due to the presence of biologically active constituents. Aloe vera is a plant endowed with therapeutic benefits especially in skincare due to its unique curative properties. The present study focused on an environmental friendly and rapid method of phytosynthesis of silver nanoparticles (Ag-NPs) using A. vera gel extract as a reductant. The synthesized Ag-NPs were characterized by transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier transform infrared (FTIR), and dynamic light scattering (DLS). TEM micrographs showed spherical-shaped synthesized Ag-NPs with a diameter of 50–100 nm. The UV-Vis spectrum displayed a broad absorption peak of surface plasmon resonance (SPR) at 450 nm. The mean size and size distribution of the formed Ag-NPs were investigated using the DLS technique. Antibacterial studies revealed zones of inhibition by Ag-NPs of A. vera (9 and 7 mm) against Pseudomonas aeruginosa and Escherichia coli, respectively. Furthermore, the antifungal activity was screened, based on the diameter of the growth inhibition zone using the synthesized Ag-NPs for different fungal strains. Anticancer activity of the synthesized Ag-NPs against the mouse melanoma F10B16 cell line revealed 100% inhibition with Ag-NPs at a concentration of 100 µg mL−1. The phytosynthesized Ag-NPs demonstrated a marked antimicrobial activity and also exhibited a potent cytotoxic effect against mouse melanoma F10B16 cells. The key findings of this study indicate that synthesized Ag-NPs exhibit profound therapeutic activity and could be potentially ideal alternatives in medicinal applications.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

scholarly journals Biophysical and Characterizations of Silver Nanoparticles used as Salmonella typhi Detector

2014 ◽  
Vol 2 (4) ◽  
pp. 510-515
Author(s):  
Hala Moustafa Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Combined Action ◽  
Hog Plum ◽  
Transmission Electronmicroscopy

The present study mainly focuses of combined action of Nepali hog plum as well as citrate synthesized silver nanoparticles (AgNPs) and Amikacin, as an antibiotic. The synergistic actions of citrate stabilized silver nanoparticles (AgNPs with chem) were compared with that of Nepali hog plum Choerospondia saxillaris (Lapsi) synthesized silver nanoparticles (AgNPs with plant), together with action of antibiotic onselected bacterial strains of Salmonella typhi. The synthesized AgNPs were characterized through UV-Vis spectroscopy, Transmission electronmicroscopy and X-ray diffraction technique. The size of the synthesized silver nanoparticles was measured by Transmission Electron Microscope (TEM) and X-ray diffraction (XRD).DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11127 Int J Appl Sci Biotechnol, Vol. 2(4): 510-515 

scholarly journals Nanocatalytic Activity of Silver Nanoparticles (Ag-Nps) Fabricated using Camellia sinensis (Linn) Tender Leaf Extract and their Characterization

2020 ◽  
Vol 2 (1) ◽  
pp. 24
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Pancreatic Enzyme ◽  
Alpha Amylase ◽  
Assay Method ◽  
Ag Nps ◽  
Visible Absorption ◽  
Biological Reduction ◽  
Vis Spectroscopy ◽  
Uv Visible

Silver nanoparticles (Ag-NPs) were prepared by the biological reduction method. Green tea extract was taken as a reducing and stabilizing agent and silver nitrate as the metal precursor for nanoparticle synthesis. The formation of the silver nanoparticles was monitored visually and using UV-Visible absorption spectroscopy. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, FTIR, Zeta sizer, Zeta potential, and antimicrobial studies. Silver nanoparticles were also subjected to investigate nanocatalytic activity with standard pancreatic alpha-amylase and bacterial amylase enzyme by the DNS assay method. UV-Vis spectroscopy revealed the formation of silver nanoparticles by exhibiting the typical surface plasmon absorption maxima at 430 nm. Four major functional groups of bio-molecules such as phenol, carboxylic acid, protein, and alkyl group were recorded in FTIR spectra. The size of the nanoparticles ranges between 5nm and 150nm. The average size and size distribution of silver nanoparticles is 59.66nm. The zeta potential of the silver nanoparticle is negatively charged and rendered as a sharp peak at -31.7mV. Antimicrobial activity of silver nanoparticles exhibited the highest inhibition against Gram-negative bacteria than Gram-positive bacteria and yeast pathogens. Starch hydrolysis of Ag-NPs was studied with pancreatic alpha-amylase (tailor made), crude and purified bacterial amylase enzyme. The formation of reducing sugar was increased about 40-fold for a purified enzyme, 11-fold for the pancreatic enzyme, and 6-fold for crude bacterial enzyme incorporated with Ag-NPs over control. The present studies recommended that Ag-NPs have a significant role in the degradation of starch into reducing sugars by acting as a nanocatalyst.

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