scholarly journals Nanohydroxyapatite Electrodeposition onto Electrospun Nanofibers: Technique Overview and Tissue Engineering Applications

2021 ◽  
Vol 8 (11) ◽  
pp. 151
Author(s):  
Thiago Domingues Stocco ◽  
Pedro José Gomes Rodrigues ◽  
Mauricio Augusto de Almeida Filho ◽  
Anderson Oliveira Lobo
Keyword(s):  
Tissue Engineering ◽  
Bone Growth ◽  
Low Cost ◽  
Electrospun Nanofibers ◽  
Short Review ◽  
Nanofibrous Scaffolds ◽  
Polymeric Nanofibers ◽  
New Methodologies ◽  
Future Direction ◽  
Nanocomposite Scaffolds

Nanocomposite scaffolds based on the combination of polymeric nanofibers with nanohydroxyapatite are a promising approach within tissue engineering. With this strategy, it is possible to synthesize nanobiomaterials that combine the well-known benefits and advantages of polymer-based nanofibers with the osteointegrative, osteoinductive, and osteoconductive properties of nanohydroxyapatite, generating scaffolds with great potential for applications in regenerative medicine, especially as support for bone growth and regeneration. However, as efficiently incorporating nanohydroxyapatite into polymeric nanofibers is still a challenge, new methodologies have emerged for this purpose, such as electrodeposition, a fast, low-cost, adjustable, and reproducible technique capable of depositing coatings of nanohydroxyapatite on the outside of fibers, to improve scaffold bioactivity and cell–biomaterial interactions. In this short review paper, we provide an overview of the electrodeposition method, as well as a detailed discussion about the process of electrodepositing nanohydroxyapatite on the surface of polymer electrospun nanofibers. In addition, we present the main findings of the recent applications of polymeric micro/nanofibrous scaffolds coated with electrodeposited nanohydroxyapatite in tissue engineering. In conclusion, comments are provided about the future direction of nanohydroxyapatite electrodeposition onto polymeric nanofibers.

scholarly journals Current progress in application of polymeric nanofibers to tissue engineering

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Sorour Nemati ◽  
Se-jeong Kim ◽  
Young Min Shin ◽  
Heungsoo Shin
Keyword(s):  
Tissue Engineering ◽  
Cell Biology ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
High Specific Surface Area ◽  
Structural Morphology ◽  
Nanofibrous Scaffolds ◽  
Nanofibrous Structure ◽  
Polymeric Nanofibers ◽  
Solution Parameters

Abstract Tissue engineering uses a combination of cell biology, chemistry, and biomaterials to fabricate three dimensional (3D) tissues that mimic the architecture of extracellular matrix (ECM) comprising diverse interwoven nanofibrous structure. Among several methods for producing nanofibrous scaffolds, electrospinning has gained intense interest because it can make nanofibers with a porous structure and high specific surface area. The processing and solution parameters of electrospinning can considerably affect the assembly and structural morphology of the fabricated nanofibers. Electrospun nanofibers can be made from natural or synthetic polymers and blending them is a straightforward way to tune the functionality of the nanofibers. Furthermore, the electrospun nanofibers can be functionalized with various surface modification strategies. In this review, we highlight the latest achievements in fabricating electrospun nanofibers and describe various ways to modify the surface and structure of scaffolds to promote their functionality. We also summarize the application of advanced polymeric nanofibrous scaffolds in the regeneration of human bone, cartilage, vascular tissues, and tendons/ligaments.

scholarly journals Electrospun Nanofibers Applications in Dentistry

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Seog-Jin Seo ◽  
Hae-Won Kim ◽  
Jung-Hwan Lee
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Pore Size ◽  
Surface Functionalization ◽  
High Surface ◽  
High Surface Area ◽  
Electrospun Nanofibers ◽  
Fiber Alignment ◽  
Cell Homing ◽  
Polymeric Nanofibers

Nanofibrous structures exhibit many interesting features, such as high surface area and surface functionalization and porosity in the range from submicron to nanoscale, which mimics the natural extracellular matrix. In particular, electrospun nanofibers have gained great attention in the field of tissue engineering due to the ease of fabrication and tailorability in pore size, scaffold shape, and fiber alignment. For the reasons, recently, polymeric nanofibers or bioceramic nanoparticle-incorporated nanofibers have been used in dentistry, and their nanostructure and flexibility have contributed to highly promotive cell homing behaviors, resulting in expecting improved dental regeneration. Here, this paper focuses on recently applied electrospun nanofibers in dentistry in the range from the process to the applications.

scholarly journals A Mini-Review: Needleless Electrospinning of Nanofibers for Pharmaceutical and Biomedical Applications

Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 673 ◽  
Author(s):  
Ioannis Partheniadis ◽  
Ioannis Nikolakakis ◽  
Ivo Laidmäe ◽  
Jyrki Heinämäki
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Biomedical Applications ◽  
Research Work ◽  
Production Capacity ◽  
Electrospun Nanofibers ◽  
Review Article ◽  
Full Potential ◽  
Needleless Electrospinning ◽  
Polymeric Nanofibers

Electrospinning (ES) is a convenient and versatile method for the fabrication of nanofibers and has been utilized in many fields including pharmaceutical and biomedical applications. Conventional ES uses a needle spinneret for the generation of nanofibers and is associated with many limitations and drawbacks (i.e., needle clogging, limited production capacity, and low yield). Needleless electrospinning (NLES) has been proposed to overcome these problems. Within the last two decades (2004–2020), many research articles have been published reporting the use of NLES for the fabrication of polymeric nanofibers intended for drug delivery and biomedical tissue engineering applications. The objective of the present mini-review article is to elucidate the potential of NLES for designing such novel nanofibrous drug delivery systems and tissue engineering constructs. This paper also gives an overview of the key NLES approaches, including the most recently introduced NLES method: ultrasound-enhanced electrospinning (USES). The technologies underlying NLES systems and an evaluation of electrospun nanofibers are presented. Even though NLES is a promising approach for the industrial production of nanofibers, it is a multivariate process, and more research work is needed to elucidate its full potential and limitations.

scholarly journals Hydrophilic Surface Functionalization of Electrospun Nanofibrous Scaffolds in Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2636
Author(s):  
Beata Niemczyk-Soczynska ◽  
Arkadiusz Gradys ◽  
Pawel Sajkiewicz
Keyword(s):  
Tissue Engineering ◽  
Surface Functionalization ◽  
Physical Adsorption ◽  
Electrospun Nanofibers ◽  
Biologically Active ◽  
Bioactive Molecules ◽  
Layer By Layer ◽  
Hydrophilic Surface ◽  
Nanofibrous Scaffolds ◽  
Covalent Grafting

Electrospun polymer nanofibers have received much attention in tissue engineering due to their valuable properties such as biocompatibility, biodegradation ability, appropriate mechanical properties, and, most importantly, fibrous structure, which resembles the morphology of extracellular matrix (ECM) proteins. However, they are usually hydrophobic and suffer from a lack of bioactive molecules, which provide good cell adhesion to the scaffold surface. Post-electrospinning surface functionalization allows overcoming these limitations through polar groups covalent incorporation to the fibers surface, with subsequent functionalization with biologically active molecules or direct deposition of the biomolecule solution. Hydrophilic surface functionalization methods are classified into chemical approaches, including wet chemical functionalization and covalent grafting, a physiochemical approach with the use of a plasma treatment, and a physical approach that might be divided into physical adsorption and layer-by-layer assembly. This review discusses the state-of-the-art of hydrophilic surface functionalization strategies of electrospun nanofibers for tissue engineering applications. We highlighted the major advantages and drawbacks of each method, at the same time, pointing out future perspectives and solutions in the hydrophilic functionalization strategies.

scholarly journals Polymer fibers produced by electrospinning technique

2019 ◽  
Vol 1 (1) ◽  
pp. 6-7
Keyword(s):  
Organic Dyes ◽  
Low Cost ◽  
Electrospun Nanofibers ◽  
Point Of View ◽  
Polymer Fibers ◽  
Polymer Nanofibers ◽  
Electrospinning Technique ◽  
Simple Method ◽  
Polymeric Nanofibers ◽  
Doped Polymers

Electrospinning technique is a very simple method for producing polymer fibers with nano and micrometric diameters. The process is scalable, flexible and low-cost because does not require complicated devices or high temperatures. Thus, fine polymers’ fibers are drawn from solutions by applying a high electric field. These polymer fibers can be further functionalized using nanoparticles’ addition or further chemical or electrochemical deposition of various compounds on the surface of the electrospun nanofibers [1-3]. Functionalized meshes of nanofibers obtained by the electrospinning technique have been successfully used as thermochromic, magnetochromic, and electroluminescent devices. Such nanofibers were prove to be able to mimic the human muscles’ movements [4, 5]. Morphology control is allowed by the possibility of controlling all the process parameters (temperature, viscosity of polymeric solution, applied voltage, distance between electrodes, etc.). Electrospun polymeric nanofibers have multiple applications in medicine but they also permit manipulation of light at nanometric dimensions when doped with organic dyes or different nanoparticles. Dye doped polymers were studied in details, from the point of view of the emission tuning with morphology and with composition [6-9]. We present our studies regarding the tuning of the properties of polymer nanofibers produced by electrospinning. Our main objective was to produce functionalized polymer nanofibers by addition of different compounds and determine their structural, morphological and optical properties.

Electrospun Nanofibers for Diabetes: Tissue Engineering and Cell-Based Therapies

2019 ◽  
Vol 14 (2) ◽  
pp. 152-168 ◽  
Author(s):  
Elham Hoveizi ◽  
Shima Tavakol ◽  
Sadegh Shirian ◽  
Khadije Sanamiri
Keyword(s):  
Diabetes Mellitus ◽  
Tissue Engineering ◽  
Therapeutic Approach ◽  
Electrospun Nanofibers ◽  
Review Article ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Nanofibrous Scaffolds ◽  
Treatment Of Diabetes

Diabetes mellitus is an autoimmune disease which causes loss of insulin secretion producing hyperglycemia by promoting progressive destruction of pancreatic β cells. An ideal therapeutic approach to manage diabetes mellitus is pancreatic β cells replacement. The aim of this review article was to evaluate the role of nanofibrous scaffolds and stem cells in the treatment of diabetes mellitus. Various studies have pointed out that application of electrospun biomaterials has considerably attracted researchers in the field of tissue engineering. The principles of cell therapy for diabetes have been reviewed in the first part of this article, while the usability of tissue engineering as a new therapeutic approach is discussed in the second part.

scholarly journals Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1609 ◽  
Author(s):  
Simin Nazarnezhad ◽  
Francesco Baino ◽  
Hae-Won Kim ◽  
Thomas J. Webster ◽  
Saeid Kargozar
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Soft Tissues ◽  
Electrospun Nanofibers ◽  
Bioactive Molecules ◽  
Tissue Repair And Regeneration ◽  
Nanofibrous Scaffolds

Angiogenesis (or the development of new blood vessels) is a key event in tissue engineering and regenerative medicine; thus, a number of biomaterials have been developed and combined with stem cells and/or bioactive molecules to produce three-dimensional (3D) pro-angiogenic constructs. Among the various biomaterials, electrospun nanofibrous scaffolds offer great opportunities for pro-angiogenic approaches in tissue repair and regeneration. Nanofibers made of natural and synthetic polymers are often used to incorporate bioactive components (e.g., bioactive glasses (BGs)) and load biomolecules (e.g., vascular endothelial growth factor (VEGF)) that exert pro-angiogenic activity. Furthermore, seeding of specific types of stem cells (e.g., endothelial progenitor cells) onto nanofibrous scaffolds is considered as a valuable alternative for inducing angiogenesis. The effectiveness of these strategies has been extensively examined both in vitro and in vivo and the outcomes have shown promise in the reconstruction of hard and soft tissues (mainly bone and skin, respectively). However, the translational of electrospun scaffolds with pro-angiogenic molecules or cells is only at its beginning, requiring more research to prove their usefulness in the repair and regeneration of other highly-vascularized vital tissues and organs. This review will cover the latest progress in designing and developing pro-angiogenic electrospun nanofibers and evaluate their usefulness in a tissue engineering and regenerative medicine setting.

Evaluation of PCL/GE-Based Electrospun Nanofibers for Tissue Engineering and Drug Delivery Application

2014 ◽  
Vol 695 ◽  
pp. 332-335 ◽  
Author(s):  
Lor Huai Chong ◽  
Mim Mim Lim ◽  
Naznin Sultana
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Contact Angle ◽  
Electrospun Nanofibers ◽  
Processing Parameters ◽  
Attenuated Total Reflectance ◽  
Water Contact ◽  
Fourier Transformed Infrared Spectroscopy ◽  
Nanofibrous Scaffolds ◽  
Solution Parameters

Scaffold provides a suitable medium for cell growing and drug delivery while enhancing the cell transplantation efficiency. In this project, nanofibrous scaffolds were fabricated through electrospinning of Polycaprolactone (PCL) and Gelatin (GE). Processing parameters and solution parameters were optimized to achieve the desired properties of PCL/GE nanofibers. Scanning Electron Microscopy (SEM), water contact angle and Attenuated Total Reflectance–Fourier Transformed Infrared Spectroscopy (ATR-FTIR) were implemented to characterize the fabricated nanofibers. It was found that 14% w/v PCL/GE shows the best fibers’ diameter, pore size, contact angle and less bead formation. This sample is suitable to be further investigated for the application of tissue engineering (TE) and drug delivery system (DDS).

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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