scholarly journals Interaction of Cold Atmospheric Argon and Helium Plasma Jets with Bio-Target with Grounded Substrate Beneath

2019 ◽  
Vol 9 (21) ◽  
pp. 4528 ◽  
Author(s):  
Irina Schweigert ◽  
Dmitry Zakrevsky ◽  
Pavel Gugin ◽  
Elena Yelak ◽  
Ekaterina Golubitskaya ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Gas Flow ◽  
Fluid Model ◽  
Plasma Jet ◽  
Plasma Jets ◽  
Atmospheric Plasma ◽  
Maximum Efficiency ◽  
Embryonic Cells ◽  
Plasma Parameters

The cold atmospheric pressure plasma jet interaction with the bio-target is studied in the plasma experiment, 2D fluid model simulations, and with MTT and iCELLigence assays of the viability of cancer cells. It is shown, for the first time, that the use of the grounded substrate under the media with cells considerably amplifies the effect of plasma cancer cell treatment in vitro. Plasma devices with cylindrical and plane geometries generating cold atmospheric plasma jets are developed and tested. The sequence of the streamers which forms the plasma jet is initiated with a voltage of 2.5–6.5 kV applied with the frequency 40 kHz. We suggest using the grounded substrate under the bio-target during the plasma jet treatment of cancer cells. The analysis of the measured plasma spectra and comparison of OH-line intensity for different voltages and gas flow rates allows us to find a range of optimal plasma parameters for the enhanced OH generation. The time-dependent viability is measured for human cell lines, A431 (skin carcinoma), HEK 293 (kidney embryonic cells), and A549 (human lung adenocarcinoma cells) after the plasma jet treatment. The results with cell-based experiments (direct treatment) performed with various plasma jet parameters confirm the maximum efficiency of the treatment with the optimal plasma parameters.

scholarly journals Production of reactive species in alginate hydrogels for cold atmospheric plasma-based therapies

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Cédric Labay ◽  
Inès Hamouda ◽  
Francesco Tampieri ◽  
Maria-Pau Ginebra ◽  
Cristina Canal
Keyword(s):  
Plasma Treatment ◽  
Cancer Cells ◽  
Gas Flow ◽  
Isotonic Saline ◽  
Chemical Properties ◽  
Plasma Jets ◽  
The Body ◽  
Atmospheric Plasma ◽  
Fast Diffusion ◽  
Alginate Hydrogels

Abstract In the last years, great advances have been made in therapies based in cold atmospheric plasmas (CAP). CAP generate reactive oxygen and nitrogen species (RONS) which can be transferred to liquids. These CAP activated liquids display the same biological efficacy (i.e. on killing cancer cells) as CAP themselves, opening the door for minimally invasive therapies. However, injection of a liquid in the body results in fast diffusion due to extracellular fluids and blood flow. Therefore, the development of efficient vehicles which allow local confinement and delivery of RONS to the diseased site is a fundamental requirement. In this work, we investigate the generation of RONS (H2O2, NO2−, short-lived RONS) in alginate hydrogels by comparing two atmospheric pressure plasma jets: kINPen and a helium needle, at a range of plasma treatment conditions (time, gas flow, distance to the sample). The physic-chemical properties of the hydrogels remain unchanged by the plasma treatment, while the hydrogel shows several-fold larger capacity for generation of RONS than a typical isotonic saline solution. Part of the RONS are quickly released to a receptor media, so special attention has to be put on the design of hydrogels with in-situ crosslinking. Remarkably, the hydrogels show capacity for sustained release of the RONS. The plasma-treated hydrogels remain fully biocompatible (due the fact that the species generated by plasma are previously washed away), indicating that no cytotoxic modifications have occurred on the polymer. Moreover, the RONS generated in alginate solutions showed cytotoxic potential towards bone cancer cells. These results open the door for the use of hydrogel-based biomaterials in CAP-associated therapies.

scholarly journals The design and manufacture of atmospheric plasma jet surgical handpiece

2020 ◽  
Vol 13 (6) ◽  
pp. 235-240
Author(s):  
Hamed Bagheri ◽  
Reza Reiazi ◽  
Mohammad Kasaie ◽  
Hosein Mootabian
Keyword(s):  
Cancer Cells ◽  
Gas Flow ◽  
Room Temperature ◽  
Low Cost ◽  
Plasma Jet ◽  
Atmospheric Plasma ◽  
Effective Parameters ◽  
Stable Performance ◽  
Design And Manufacture ◽  
Low Cost Manufacturing

AbstractBackgroundAtmospheric plasma jet has different medical applications due to its low temperature at room temperature. In recent years, the effect of nonthermal plasmas on cancer cells has been studied, and it has been shown that this type of plasma has anti-proliferative effects on cancer cells.ObjectivesTo design a plasma jet handpiece, which can be used in cutting operations in less bleeding surgery, eliminating cancer cells without damage to healthy cells and reducing the duration of wound healing.MethodsThe plasma handpiece simply consists of a nozzle body and two cathode and anode electrodes and a fully insulated body against heat and high voltage. Argon is introduced into the handpiece, and by plasma treatment, it is used for special purposes. Each piece was made according to its own manufacturing process and by assembling; the final product of the atmospheric plasma jet handpiece was ready for testing. The jet pipeline was then tested, and the effective parameters were examined.ResultsThe cold atmospheric plasma jet length depends on factors such as power supply, applied voltage, gas flow rate and the distance between the electrodes. The results showed with increasing velocity, the flame and jet lengths decreased greatly due to high losses of plasma, including ions and electrons. Also with increasing the velocity of argon gas, its concentration decreased.ConclusionsIt is concluded that the performance of the proposed design is successful. The advantages include low-cost manufacturing, highly stable performance, and low erosion and can be considered for future development.

scholarly journals Killing of adherent oral microbes by a non-thermal atmospheric plasma jet

2010 ◽  
Vol 59 (2) ◽  
pp. 206-212 ◽  
Author(s):  
Stefan Rupf ◽  
Antje Lehmann ◽  
Matthias Hannig ◽  
Barbara Schäfer ◽  
Andreas Schubert ◽  
...  
Keyword(s):  
Plasma Jet ◽  
Plasma Jets ◽  
Atmospheric Plasma ◽  
Killing Effect ◽  
E Coli ◽  
Potential Applications ◽  
Oral Microbes ◽  
Micro Organisms

Atmospheric plasma jets are being intensively studied with respect to potential applications in medicine. The aim of this in vitro study was to test a microwave-powered non-thermal atmospheric plasma jet for its antimicrobial efficacy against adherent oral micro-organisms. Agar plates and dentin slices were inoculated with 6 log10 c.f.u. cm−2 of Lactobacillus casei, Streptococcus mutans and Candida albicans, with Escherichia coli as a control. Areas of 1 cm2 on the agar plates or the complete dentin slices were irradiated with a helium plasma jet for 0.3, 0.6 or 0.9 s mm−2, respectively. The agar plates were incubated at 37 °C, and dentin slices were vortexed in liquid media and suspensions were placed on agar plates. The killing efficacy of the plasma jet was assessed by counting the number of c.f.u. on the irradiated areas of the agar plates, as well as by determination of the number of c.f.u. recovered from dentin slices. A microbe-killing effect was found on the irradiated parts of the agar plates for L. casei, S. mutans, C. albicans and E. coli. The plasma-jet treatment reduced the c.f.u. by 3–4 log10 intervals on the dentin slices in comparison to recovery rates from untreated controls. The microbe-killing effect was correlated with increasing irradiation times. Thus, non-thermal atmospheric plasma jets could be used for the disinfection of dental surfaces.

Activation of deionized water by atmospheric plasma jet in helium gas flow

2021 ◽  
Author(s):  
P. Y. Tan ◽  
O. H. Chin ◽  
R. Anpalagan ◽  
Y. T. Lau ◽  
H. C. Lee
Keyword(s):  
Gas Flow ◽  
Plasma Jet ◽  
Deionized Water ◽  
Atmospheric Plasma ◽  
Helium Gas

scholarly journals Tiny Cold Atmospheric Plasma Jet for Biomedical Applications

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 249
Author(s):  
Zhitong Chen ◽  
Richard Obenchain ◽  
Richard E. Wirz
Keyword(s):  
Biomedical Applications ◽  
Plasma Jet ◽  
Discharge Voltage ◽  
Plasma Jets ◽  
Atmospheric Plasma ◽  
Physical Parameters ◽  
Cold Atmospheric Plasma ◽  
Water Metal ◽  
Plasma Probe ◽  
Jet Nozzle

Conventional plasma jets for biomedical applications tend to have several drawbacks, such as high voltages, high gas delivery, large plasma probe volume, and the formation of discharge within the organ. Therefore, it is challenging to employ these jets inside a living organism’s body. Thus, we developed a single-electrode tiny plasma jet and evaluated its use for clinical biomedical applications. We investigated the effect of voltage input and flow rate on the jet length and studied the physical parameters of the plasma jet, including discharge voltage, average gas and subject temperature, and optical emissions via spectroscopy (OES). The interactions between the tiny plasma jet and five subjects (de-ionized (DI) water, metal, cardboard, pork belly, and pork muscle) were studied at distances of 10 mm and 15 mm from the jet nozzle. The results showed that the tiny plasma jet caused no damage or burning of tissues, and the ROS/RNS (reactive oxygen/nitrogen species) intensity increased when the distance was lowered from 15 mm to 10 mm. These initial observations establish the tiny plasma jet device as a potentially useful tool in clinical biomedical applications.

scholarly journals Parameters Affecting the Antimicrobial Properties of Cold Atmospheric Plasma Jet

2019 ◽  
Vol 8 (11) ◽  
pp. 1930 ◽  
Author(s):  
Bih-Show Lou ◽  
Chih-Ho Lai ◽  
Teng-Ping Chu ◽  
Jang-Hsing Hsieh ◽  
Chun-Ming Chen ◽  
...  
Keyword(s):  
Gas Flow ◽  
Treatment Time ◽  
Plasma Jet ◽  
Atmospheric Plasma ◽  
Gas Flow Rate ◽  
Cold Atmospheric Plasma ◽  
E Coli ◽  
Antimicrobial Efficiency ◽  
Ar Gas ◽  
Sample Distance

Using the Taguchi method to narrow experimental parameters, the antimicrobial efficiency of a cold atmospheric plasma jet (CAPJ) treatment was investigated. An L9 array with four parameters of CAPJ treatments, including the application voltage, CAPJ-sample distance, argon (Ar) gas flow rate, and CAPJ treatment time, were applied to examine the antimicrobial activity against Escherichia coli (E. coli). CAPJ treatment time was found to be the most influential parameter in its antimicrobial ability by evaluation of signal to noise ratios and analysis of variance. 100% bactericidal activity was achieved under the optimal bactericidal activity parameters including the application voltage of 8.5 kV, CAPJ-sample distance of 10 mm, Ar gas flow rate of 500 sccm, and CAPJ treatment time of 300 s, which confirms the efficacy of the Taguchi method in this design. In terms of the mechanism of CAPJ’s antimicrobial ability, the intensity of hydroxyl radical produced by CAPJ positively correlated to its antimicrobial efficiency. The CAPJ antimicrobial efficiency was further evaluated by both DNA double-strand breaks analysis and scanning electron microscopy examination of CAPJ treated bacteria. CAPJ destroyed the cell wall of E. coli and further damaged its DNA structure, thus leading to successful killing of bacteria. This study suggests that optimal conditions of CPAJ can provide effective antimicrobial activity and may be grounds for a novel approach for eradicating bacterial infections.

scholarly journals Cold Plasma-Treated Ringer’s Saline: A Weapon to Target Osteosarcoma

Cancers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 227 ◽  
Author(s):  
Miguel Mateu-Sanz ◽  
Juan Tornín ◽  
Bénédicte Brulin ◽  
Anna Khlyustova ◽  
Maria-Pau Ginebra ◽  
...  
Keyword(s):  
Culture Media ◽  
Reactive Oxygen ◽  
Plasma Jets ◽  
Atmospheric Plasma ◽  
Nitrogen Species ◽  
Organotypic Cultures ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Cold Plasmas

Osteosarcoma (OS) is the main primary bone cancer, presenting poor prognosis and difficult treatment. An innovative therapy may be found in cold plasmas, which show anti-cancer effects related to the generation of reactive oxygen and nitrogen species in liquids. In vitro models are based on the effects of plasma-treated culture media on cell cultures. However, effects of plasma-activated saline solutions with clinical application have not yet been explored in OS. The aim of this study is to obtain mechanistic insights on the action of plasma-activated Ringer’s saline (PAR) for OS therapy in cell and organotypic cultures. To that aim, cold atmospheric plasma jets were used to obtain PAR, which produced cytotoxic effects in human OS cells (SaOS-2, MG-63, and U2-OS), related to the increasing concentration of reactive oxygen and nitrogen species generated. Proof of selectivity was found in the sustained viability of hBM-MSCs with the same treatments. Organotypic cultures of murine OS confirmed the time-dependent cytotoxicity observed in 2D. Histological analysis showed a decrease in proliferating cells (lower Ki-67 expression). It is shown that the selectivity of PAR is highly dependent on the concentrations of reactive species, being the differential intracellular reactive oxygen species increase and DNA damage between OS cells and hBM-MSCs key mediators for cell apoptosis.

Properties and in vitro assessment of ZrO2-based coatings obtained by atmospheric plasma jet spraying on biodegradable Mg-Ca and Mg-Ca-Zr alloys

2020 ◽  
Vol 46 (10) ◽  
pp. 15897-15906 ◽  
Author(s):  
Bogdan Istrate ◽  
Julietta V. Rau ◽  
Corneliu Munteanu ◽  
Iulian V. Antoniac ◽  
Vicentiu Saceleanu
Keyword(s):  
Plasma Jet ◽  
Atmospheric Plasma ◽  
In Vitro Assessment ◽  
Zr Alloys

scholarly journals In vitro characterization of two different atmospheric plasma jet chemical functionalizations of titanium surfaces

2017 ◽  
Vol 409 ◽  
pp. 314-324 ◽  
Author(s):  
F. Mussano ◽  
T. Genova ◽  
E. Verga Falzacappa ◽  
P. Scopece ◽  
L. Munaron ◽  
...  
Keyword(s):  
Plasma Jet ◽  
Atmospheric Plasma ◽  
In Vitro Characterization ◽  
Titanium Surfaces

scholarly journals Mismatch of frequencies of ac voltage and streamers propagation in cold atmospheric plasma jet for typical regimes of cancer cell treatment

2021 ◽  
Vol 2100 (1) ◽  
pp. 012020
Author(s):  
I Schweigert ◽  
A Alexandrov ◽  
D Zakrevsky ◽  
E Milakhina ◽  
E Patrakova ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Plasma Jet ◽  
Plasma Source ◽  
Atmospheric Plasma ◽  
Modes Of Operation ◽  
Cold Atmospheric Plasma ◽  
Mode Of Operation ◽  
Voltage Frequency

Abstract Cold atmospheric plasma (CAP) jet generated by the plasma source at 2-6 kV ac voltages with frequencies of 10-50 kHz demonstrate the different modes of operation. Depending on the voltage frequency and amplitude, some streamers in the plasma jet are short and decay before they approach the treated surface. In this case, the effect on the viability of cancer cells when exposed to CAP jet strongly depends on the mode of operation of the discharge or, in other words, on how many times the streamers hit the bio-target during the treatment. The effect on different modes on cancer cells A549 viability is reported.

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