Activation of deionized water by atmospheric plasma jet in helium gas flow

2021 ◽  
Author(s):  
P. Y. Tan ◽  
O. H. Chin ◽  
R. Anpalagan ◽  
Y. T. Lau ◽  
H. C. Lee
2019 ◽  
Vol 8 (11) ◽  
pp. 1930 ◽  
Author(s):  
Bih-Show Lou ◽  
Chih-Ho Lai ◽  
Teng-Ping Chu ◽  
Jang-Hsing Hsieh ◽  
Chun-Ming Chen ◽  
...  

Using the Taguchi method to narrow experimental parameters, the antimicrobial efficiency of a cold atmospheric plasma jet (CAPJ) treatment was investigated. An L9 array with four parameters of CAPJ treatments, including the application voltage, CAPJ-sample distance, argon (Ar) gas flow rate, and CAPJ treatment time, were applied to examine the antimicrobial activity against Escherichia coli (E. coli). CAPJ treatment time was found to be the most influential parameter in its antimicrobial ability by evaluation of signal to noise ratios and analysis of variance. 100% bactericidal activity was achieved under the optimal bactericidal activity parameters including the application voltage of 8.5 kV, CAPJ-sample distance of 10 mm, Ar gas flow rate of 500 sccm, and CAPJ treatment time of 300 s, which confirms the efficacy of the Taguchi method in this design. In terms of the mechanism of CAPJ’s antimicrobial ability, the intensity of hydroxyl radical produced by CAPJ positively correlated to its antimicrobial efficiency. The CAPJ antimicrobial efficiency was further evaluated by both DNA double-strand breaks analysis and scanning electron microscopy examination of CAPJ treated bacteria. CAPJ destroyed the cell wall of E. coli and further damaged its DNA structure, thus leading to successful killing of bacteria. This study suggests that optimal conditions of CPAJ can provide effective antimicrobial activity and may be grounds for a novel approach for eradicating bacterial infections.


2019 ◽  
Vol 9 (21) ◽  
pp. 4528 ◽  
Author(s):  
Irina Schweigert ◽  
Dmitry Zakrevsky ◽  
Pavel Gugin ◽  
Elena Yelak ◽  
Ekaterina Golubitskaya ◽  
...  

The cold atmospheric pressure plasma jet interaction with the bio-target is studied in the plasma experiment, 2D fluid model simulations, and with MTT and iCELLigence assays of the viability of cancer cells. It is shown, for the first time, that the use of the grounded substrate under the media with cells considerably amplifies the effect of plasma cancer cell treatment in vitro. Plasma devices with cylindrical and plane geometries generating cold atmospheric plasma jets are developed and tested. The sequence of the streamers which forms the plasma jet is initiated with a voltage of 2.5–6.5 kV applied with the frequency 40 kHz. We suggest using the grounded substrate under the bio-target during the plasma jet treatment of cancer cells. The analysis of the measured plasma spectra and comparison of OH-line intensity for different voltages and gas flow rates allows us to find a range of optimal plasma parameters for the enhanced OH generation. The time-dependent viability is measured for human cell lines, A431 (skin carcinoma), HEK 293 (kidney embryonic cells), and A549 (human lung adenocarcinoma cells) after the plasma jet treatment. The results with cell-based experiments (direct treatment) performed with various plasma jet parameters confirm the maximum efficiency of the treatment with the optimal plasma parameters.


2015 ◽  
Vol 719-720 ◽  
pp. 221-230
Author(s):  
Tao Liang ◽  
Shi Ying Hou

Two kinds of multiple-pulse were reported on atmospheric plasma jet (APPJ) of dielectric barrier discharge (DBD) configurations in this study. Meanwhile, the investigation on electrical characteristics of APPJ in helium and helium/air was carried out with the aim of shedding light on the electrode parameters for dissipated power and jet length. The typical bacteria, E. coil, were employed to study the effect by the two APPJ devices (PJ-1 and PJ-2). For PJ-1, multiple-pulse appears in both sides of every half-cycle, but only in the positive half-cycle for PJ-2. A wider power electrode of PJ-1 and further distance from the nozzle to grounding electrode of PJ-2, whether in ambient helium or helium/air, are all conducive to improvement of dissipated power. With the increase of gas flow rate, the jet length will become longer at laminar flow mode, then get shorter, and a permanent to the end. Generally, the introduction of air leads a better sterilization effect, especially for PJ-1. Keywords: Atmospheric Pressure, multiple-pulse, Electrode parameters, dissipated power, jet length, plasma jet. PACS: 52.80.-s, 52.77.-j


2020 ◽  
Vol 13 (6) ◽  
pp. 235-240
Author(s):  
Hamed Bagheri ◽  
Reza Reiazi ◽  
Mohammad Kasaie ◽  
Hosein Mootabian

AbstractBackgroundAtmospheric plasma jet has different medical applications due to its low temperature at room temperature. In recent years, the effect of nonthermal plasmas on cancer cells has been studied, and it has been shown that this type of plasma has anti-proliferative effects on cancer cells.ObjectivesTo design a plasma jet handpiece, which can be used in cutting operations in less bleeding surgery, eliminating cancer cells without damage to healthy cells and reducing the duration of wound healing.MethodsThe plasma handpiece simply consists of a nozzle body and two cathode and anode electrodes and a fully insulated body against heat and high voltage. Argon is introduced into the handpiece, and by plasma treatment, it is used for special purposes. Each piece was made according to its own manufacturing process and by assembling; the final product of the atmospheric plasma jet handpiece was ready for testing. The jet pipeline was then tested, and the effective parameters were examined.ResultsThe cold atmospheric plasma jet length depends on factors such as power supply, applied voltage, gas flow rate and the distance between the electrodes. The results showed with increasing velocity, the flame and jet lengths decreased greatly due to high losses of plasma, including ions and electrons. Also with increasing the velocity of argon gas, its concentration decreased.ConclusionsIt is concluded that the performance of the proposed design is successful. The advantages include low-cost manufacturing, highly stable performance, and low erosion and can be considered for future development.


2018 ◽  
Vol 16 (6) ◽  
pp. 391-399
Author(s):  
Zulaika ABDULLAH ◽  
Siti Khadijah ZAABA ◽  
Mohammad Taufiq MUSTAFFA ◽  
Nor Aini SAIDIN ◽  
Johan Ariff MOHTAR ◽  
...  

The design of atmospheric plasma jet is basically using the combination of a noble gases, potential difference and a good dielectric material. In this work, we used quartz glass tube as a dielectric material with different inner diameter size to investigate the effect on the plasma discharge. We studied the changes of plasma discharge by observing the discharge lengths. We introduced a gas flow rate of 1000 ml/min and maximum inner diameter of dielectric material of 10 mm. Results showed that the discharge length are capable of reaching 30 mm and having various excited plasma species shown through optical emission spectrum.


2004 ◽  
Vol 449-452 ◽  
pp. 777-780 ◽  
Author(s):  
Chang Min Han ◽  
Han Shin Choi ◽  
Chang Hee Lee ◽  
Hyung Jun Kim ◽  
S.Y. Hwang

A blended NiCr-Cr2O3-Ag-BaF2/CaF2 feedstock was sprayed using an atmospheric plasma spraying process. Due to the differences in physical and thermophysical properties of each constituent, coating microstructures and resulting coating properties were largely dependent on the interactions between each constituent phase and plasma jet in view of the physics of thermal spraying. Thus, hydrogen gas flow rate was changed to affect the plasma jet characteristics such as gas enthalpy and gas thermal conductivity in this study. According to it, evolutions of chemical composition and microstructures of the as-sprayed coatings were observed. As the hydrogen gas flow rate was increased, Cr2O3 weight fraction was increased with the decrease of Ag and porosity. Vickers microhardness and bond strength of the coatings showed consistent behaviors to the phase composition and microstructures. Friction coefficient and weight loss during a pin-on-disc dry sliding wear test were measured from room temperature to 500°C at the interval of 100°C. Finally, the effects of phase fraction and coating properties on the friction and wear were investigated.


Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 249
Author(s):  
Zhitong Chen ◽  
Richard Obenchain ◽  
Richard E. Wirz

Conventional plasma jets for biomedical applications tend to have several drawbacks, such as high voltages, high gas delivery, large plasma probe volume, and the formation of discharge within the organ. Therefore, it is challenging to employ these jets inside a living organism’s body. Thus, we developed a single-electrode tiny plasma jet and evaluated its use for clinical biomedical applications. We investigated the effect of voltage input and flow rate on the jet length and studied the physical parameters of the plasma jet, including discharge voltage, average gas and subject temperature, and optical emissions via spectroscopy (OES). The interactions between the tiny plasma jet and five subjects (de-ionized (DI) water, metal, cardboard, pork belly, and pork muscle) were studied at distances of 10 mm and 15 mm from the jet nozzle. The results showed that the tiny plasma jet caused no damage or burning of tissues, and the ROS/RNS (reactive oxygen/nitrogen species) intensity increased when the distance was lowered from 15 mm to 10 mm. These initial observations establish the tiny plasma jet device as a potentially useful tool in clinical biomedical applications.


Author(s):  
Jie Shen ◽  
Cheng Cheng ◽  
Shidong Fang ◽  
Hongbing Xie ◽  
Longwei Cheng ◽  
...  

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