scholarly journals Simulation and Evaluation of the Radiation Dose Deposited in Human Tissues by Atmospheric Neutrons

2021 ◽  
Vol 11 (18) ◽  
pp. 8338
Author(s):  
Ernesto Ortiz ◽  
Blanca Mendoza ◽  
Carlos Gay ◽  
Victor Manuel Mendoza ◽  
Marni Pazos ◽  
...  
Keyword(s):  
Radiation Dose ◽  
Bone Tissue ◽  
Brain Tissue ◽  
Tissue Sample ◽  
Cosmic Ray ◽  
The Other ◽  
High Mountain ◽  
Human Tissues ◽  
Tissue Samples ◽  
Simulation And Evaluation

The evaluation of the radiation dose (RD) deposited by atmospheric neutrons in human tissues is of vital importance due to the potential damages that over exposure to this radiation may cause to human health. The goal of this work was to obtain the RD that atmospheric neutrons with energy from 1 to 1000 MeV deposit in tissues of the human body (blood, adipose, bone and brain) as a function of both altitude and latitude. With the help of the Geant4 software, we developed a numerical simulation that allowed us to reach our goal; atmospheric neutron fluxes were obtained from the Excel-Based Program for Calculating Atmospheric Cosmic-Ray Spectrum (EXPACS). We found that the RD deposited by atmospheric neutrons increases with the increase in altitude and latitude, e.g., for an altitude of high mountain (4 km), the RD is increased ∼19 times; while, for an altitude of commercial flights (10 km), the RD is increased ∼156 times; in both cases, regarding the RD at sea level. We also found that, in the range of energies from 1 to 100 MeV, the RD deposited in the bone tissue sample is considerably lower that the RD deposited in the blood, adipose and brain tissue samples. On the other hand, for the range of energies between 200 and 1000 MeV, the RD deposited in the bone tissue sample is considerably greater that the RD deposited in the blood, adipose and brain tissue samples.

scholarly journals The Transillumination Possibility of Imidazole–Osmium Postfixed Tissue and Its Consequences for the Handling of Tissue Samples

2005 ◽  
Vol 11 (1) ◽  
pp. 42-45 ◽  
Author(s):  
Tilman Voigt ◽  
Wolfgang Dauber
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Tissue Sample ◽  
The Other ◽  
Light Sources ◽  
Routine Procedure ◽  
Tissue Samples ◽  
Transmission Electron ◽  
The One ◽  
Point Light

Osmium postfixation is established as a routine procedure for transmission electron microscopy (TEM). On the one hand, this routine procedure leads to good results for TEM, but on the other hand results in blackened tissue samples that do not allow examination of any structures within the embedded tissue sample by a light microscope. Equivalent fixation results for TEM are achieved with imidazole–osmium postfixation, and with this postfixation method tissue is not blackened and can be transilluminated with point light sources. This allows easier recognition of histological details within tissue samples and makes it possible to screen embedded samples for appropriate ultrastructural processing. Jejunum is used to demonstrate the method.

scholarly journals Dois procedimentos para isolar sinaptossomas usando gradientes de Percoll

1994 ◽  
Vol 16 (16) ◽  
pp. 29
Author(s):  
João Batista Teixeira da Rocha ◽  
Carlos Fernando de Mello ◽  
Ana Maria Oliveira Battastini ◽  
Renato Dutra Dias ◽  
Rodrigo Bainy Leal
Keyword(s):  
Tissue Sample ◽  
Present Report ◽  
The Other ◽  
Tissue Samples ◽  
Fixed Angle ◽  
Synaptosomal Fraction ◽  
Small Tissue ◽  
Percoll Gradients

Recently, Percoll gradients have been successfully used for the isolation of synaptosomes. Te present report describes two procedures for isolating synaptosomes using discontinous Percoll gradients. One of them uses a superspeed centrifuge (Sorvall RC 2B, fixed-angle rotor SS-34), while the other uses an ultracentrifuge swinging bucket rotor (SW 50.1). Using this swinging rotor synaptosomes can be obtained from small tissue samples (100 mg). The partial caracterization of the fractions resulting from gradients demonstrated that intact synaptosomes were recovered from the 10%/15% and 10/16% Percoll interphases for the superspeed and ultracentrifuge, respectively. The mitochondrial contamination of the synaptosomal fractions was similar in both procedures and also similar to those reported in literature. The membrane contamination of the synaptosomal fraction obtained using the swinging roto r was very low, while the use of SS-34 roto r yield a fraction more contaminated with membranes. The results of the present study demonstrate that synaptosomes can be obtained by using relatively simple centrifuges (superspeed) or from small tissue sample (swinging rotor SW 50.1). The time of preparation range from 20 to 60 minutes.

VARIATION IN FATTY ACID COMPOSITION AMONG LIVER, PERIRENAL FAT, AND RIB TISSUES OF STEER CARCASSES

1965 ◽  
Vol 45 (1) ◽  
pp. 29-32 ◽  
Author(s):  
W. K. Roberts
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Bone Tissue ◽  
Palmitoleic Acid ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Tissue Samples ◽  
Arachidonic Acids ◽  
Perirenal Fat

Tissue samples (liver, perirenal fat, and non-bone tissue from 9th to 11th rib cut) were obtained from carcasses of Hereford steers that had been fattened in two different groups during two consecutive years, and were analyzed for fatty acids by gas–liquid chromatography. Myristic acid was significantly (P < 0.01) lower in liver than perirenal or rib tissues (non-bone tissue from 9th to 11th rib cut). Palmitic acid levels were significantly (P < 0.01) different among the tissues in one group, but not in the other. Palmitoleic acid was found in all areas with the highest level, within groups, occurring in rib tissue (av. 4.8%). Perirenal fat contained significantly (P < 0.01) more stearic acid than either rib or liver tissues and oleic acid was significantly (P < 0.01) higher in rib than in the other tissues. The liver tissue contained approximately 9.1% linoleic acid, which was significantly (P < 0.01) higher in perirenal or rib tissues (less than 2%). Linolenic and arachidonic acids were detected in liver, but not in the other tissues.

scholarly journals Prognostic Value of 5-ALA Fluorescence, Tumor Cell Infiltration and Angiogenesis in the Peritumoral Brain Tissue of Brain Metastases

Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 603
Author(s):  
Petra A. Mercea ◽  
Mario Mischkulnig ◽  
Barbara Kiesel ◽  
Lisa I. Wadiura ◽  
Thomas Roetzer ◽  
...  
Keyword(s):  
Brain Metastases ◽  
Tumor Cell ◽  
Brain Tissue ◽  
Aminolevulinic Acid ◽  
Tissue Sample ◽  
Cell Infiltration ◽  
Tissue Samples ◽  
Local Progression ◽  
Patient Prognosis ◽  
One Year

Complete resection is an indispensable treatment option in the management of brain metastases (BM). 5-aminolevulinic acid (5-ALA) fluorescence is used for improved intraoperative visualization of tumor tissue in gliomas and was recently observed in BM. We investigated the potential of 5-ALA fluorescence to visualize the infiltrative growth of BM in the peritumoral brain tissue and its histopathological correlate. Patients with BM resection after 5-ALA administration and collection of tissue samples from peritumoral brain tissue were included. Each tissue sample was histopathologically investigated for tumor cell infiltration and angiogenesis. Altogether, 88 samples were collected from the peritumoral brain tissue in 58 BM of 55 patients. Visible 5-ALA fluorescence was found in 61 (69%) of the samples, tumor infiltration in 19 (22%) and angiogenesis in 13 (15%) of samples. Angiogenesis showed a significant correlation with presence of fluorescence (p = 0.008). Moreover, angiogenesis was related to visible 5-ALA fluorescence and showed an association with patient prognosis since it was significantly correlated to shorter time to local progression/recurrence (p = 0.001) and lower one-year survival (p = 0.031). Consequently, angiogenesis in the peritumoral brain tissue of BM might be a novel prognostic marker for individualized perioperative treatment concepts in the future.

Immunoelectron microscopic localization of elastic tissue in archival material using an improved method

1990 ◽  
Vol 48 (3) ◽  
pp. 794-795
Author(s):  
J. C. Fanning ◽  
J. F. White ◽  
R. Polewski ◽  
E. G. Cleary
Keyword(s):  
Normal Animal ◽  
Animal Tissue ◽  
Elastic Tissue ◽  
Human Tissues ◽  
Improved Method ◽  
Tissue Samples ◽  
Archival Material ◽  
Immuno Electron Microscopy ◽  
Arteries And Veins ◽  
Biochemical Examination

Elastic tissue is an important component of the walls of arteries and veins, of skin, of the lungs and in lesser amounts, of many other tissues. It is responsible for the rubber-like properties of the arteries and for the normal texture of young skin. It undergoes changes in a number of important diseases such as atherosclerosis and emphysema and on exposure of skin to sunlight.We have recently described methods for the localizationof elastic tissue components in normal animal and human tissues. In the study of developing and diseased tissues it is often not possible to obtain samples which have been optimally prepared for immuno-electron microscopy. Sometimes there is also a need to examine retrospectively samples collected some years previously. We have therefore developed modifications to our published methods to allow examination of human and animal tissue samples obtained at surgery or during post mortem which have subsequently been: 1. stored frozen at -35° or -70°C for biochemical examination; 2.

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

scholarly journals Safety profile of the transcription factor EB (TFEB)-based gene therapy through intracranial injection in mice

2020 ◽  
Vol 11 (1) ◽  
pp. 241-250
Author(s):  
Zhenyu Li ◽  
Guangqian Ding ◽  
Yudi Wang ◽  
Zelong Zheng ◽  
Jianping Lv
Keyword(s):  
Gene Therapy ◽  
Transcription Factor ◽  
Neurodegenerative Diseases ◽  
Brain Tissue ◽  
Inflammatory Responses ◽  
Tissue Samples ◽  
Protein Levels ◽  
Virus Serotype ◽  
Transcription Factor Eb ◽  
The Brain

AbstractTranscription factor EB (TFEB)-based gene therapy is a promising therapeutic strategy in treating neurodegenerative diseases by promoting autophagy/lysosome-mediated degradation and clearance of misfolded proteins that contribute to the pathogenesis of these diseases. However, recent findings have shown that TFEB has proinflammatory properties, raising the safety concerns about its clinical application. To investigate whether TFEB induces significant inflammatory responses in the brain, male C57BL/6 mice were injected with phosphate-buffered saline (PBS), adeno-associated virus serotype 8 (AAV8) vectors overexpressing mouse TFEB (pAAV8-CMV-mTFEB), or AAV8 vectors expressing green fluorescent proteins (GFPs) in the barrel cortex. The brain tissue samples were collected at 2 months after injection. Western blotting and immunofluorescence staining showed that mTFEB protein levels were significantly increased in the brain tissue samples of mice injected with mTFEB-overexpressing vectors compared with those injected with PBS or GFP-overexpressing vectors. pAAV8-CMV-mTFEB injection resulted in significant elevations in the mRNA and protein levels of lysosomal biogenesis indicators in the brain tissue samples. No significant changes were observed in the expressions of GFAP, Iba1, and proinflammation mediators in the pAAV8-CMV-mTFEB-injected brain compared with those in the control groups. Collectively, our results suggest that AAV8 successfully mediates mTFEB overexpression in the mouse brain without inducing apparent local inflammation, supporting the safety of TFEB-based gene therapy in treating neurodegenerative diseases.

scholarly journals Genes associated with inflammation and bone remodeling are highly expressed in the bone of patients with the early-stage cam-type femoroacetabular impingement

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Guanying Gao ◽  
Ruiqi Wu ◽  
Rongge Liu ◽  
Jianquan Wang ◽  
Yingfang Ao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Tissue ◽  
Bone Remodeling ◽  
Femoroacetabular Impingement ◽  
Early Stage ◽  
Control Group ◽  
Tissue Samples ◽  
Expression Levels ◽  
Normal Bone ◽  
Impingement Zone

Abstract Background Recent studies have shown high expression levels of certain inflammatory, anabolic, and catabolic genes in the articular cartilage from the impingement zone of the hips with femoroacetabular impingement (FAI), representing an increased metabolic state. Nevertheless, little is known about the molecular properties of bone tissue from the impingement zone of hips with FAI. Methods Bone tissue samples from patients with early-stage cam-type FAI were collected during hip arthroscopy for treatment of cam-type FAI. Control bone tissue samples were collected from six patients who underwent total hip replacement because of a femoral neck fracture. Quantitative real-time polymerase chain reaction (PCR) was performed to determine the gene expression associated with inflammation and bone remodeling. The differences in the gene expression in bone tissues from the patients with early-stage cam-type FAI were also evaluated based on clinical parameters. Results In all, 12 patients with early-stage cam-type FAI and six patients in the control group were included in this study. Compared to the control samples, the bone tissue samples from patients with FAI showed higher expression levels of interleukin-6 (IL-6), alkaline phosphatase (ALP), receptor activator of nuclear factor-kB ligand (RANKL), and osteoprotegerin (OPG) (P < 0.05). IL-1 expression was detected only in the control group. On the other hand, there was no significant difference in IL-8 expression between the patients with FAI and the control group. The patients with FAI having a body mass index (BMI) of >24 kg/m2 showed higher ALP expression (P < 0.05). Further, the expression of IL-6 and ALP was higher in the patients with FAI in whom the lateral center-edge angle was >30° (P < 0.05). Conclusions Our results indicated the metabolic condition of bone tissues in patients with early-stage cam-type FAI differed from that of normal bone in the femoral head-neck junction. The expression levels of the genes associated with inflammation and bone remodeling were higher in the bone tissue of patients with early-stage cam-type FAI than in the patients with normal bone tissue.

scholarly journals The Dutch National TissueArchive Portal enables efficient, consistent, and transparent procurement of diagnostic tissue samples for scientific use

2021 ◽  
Author(s):  
Robin Verjans ◽  
Annette H. Bruggink ◽  
Robby Kibbelaar ◽  
Jos Bart ◽  
Aletta Debernardi ◽  
...  
Keyword(s):  
The Netherlands ◽  
Tissue Sample ◽  
Ffpe Tissue ◽  
Tissue Samples ◽  
Internet Portal ◽  
Formalin Fixed Paraffin ◽  
Practical Support ◽  
Formalin Fixed Paraffin Embedded ◽  
The Rich ◽  
Formalin Fixed

AbstractBiobanks play a crucial role in enabling biomedical research by facilitating scientific use of valuable human biomaterials. The PALGA foundation—a nationwide network and registry of histo- and cytopathology in the Netherlands—was established to promote the provision of data within and between pathology departments, and to make the resulting knowledge available for healthcare. Apart from the pathology data, we aimed to utilize PALGA’s nationwide network to find and access the rich wealth of Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples for scientific use.  We implemented the Dutch National TissueArchive Portal (DNTP) to utilize PALGA’s nationwide network for requesting FFPE tissue samples. The DNTP consists of (1) a centrally organized internet portal to improve the assessing, processing, harmonization, and monitoring of the procurement process, while (2) dedicated HUB-employees provide practical support at peripheral pathology departments. Since incorporation of the DNTP, both the number of filed requests for FFPE tissue samples and the amount of HUB-mediated support increased 55 and 29% respectively. In line, the sample procurement duration time decreased significantly (− 47%). These findings indicate that implementation of the DNTP improved the frequency, efficiency, and transparency of FFPE tissue sample procurement for research in the Netherlands. To conclude, the need for biological resources is growing persistently to enable precision medicine. Here, we access PALGA’s national, pathology network by implementation of the DNTP to allow for efficient, consistent, and transparent exchange of FFPE tissue samples for research across the Netherlands.

scholarly journals Reactivation of BK Polyomavirus in Urine Cytology Is Not Associated with Urothelial Cell Carcinoma

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1412
Author(s):  
Faisal Klufah ◽  
Ghalib Mobaraki ◽  
Axel zur Hausen ◽  
Iryna V. Samarska
Keyword(s):  
Cell Carcinoma ◽  
Urine Cytology ◽  
Urothelial Cell ◽  
The Other ◽  
Urothelial Cell Carcinoma ◽  
Tissue Samples ◽  
Bk Polyomavirus ◽  
Ffpe Tissues ◽  
Immunosuppressed Patients ◽  
History Of

BK polyomavirus (BKPyV) has been associated with some high-grade and special urothelial cell carcinoma (UCC) subtypes in immunosuppressed patients. Here, we evaluated the relationship of BKPyV-positive urine cytology specimens (UCS) with UCC. A large single-institution database was retrospectively searched for UCS positive for decoy cells, suggesting BKPyV infection. These were tested for the presence of BKPyV by PCR and immunohistochemistry (IHC) in urine sediments and formalin-fixed paraffin-embedded (FFPE) tissue samples of UCC. Decoy cells were reported in 30 patients out of the database with 22.867 UCS. Of these 30 patients, 16 (53.3%) had no history of UCC. Six patients out of these 16 had a history of transplantation, 4 had a history of severe chronic medical conditions, and 6 had no chronic disease. The other fourteen patients were diagnosed with either in situ or invasive UCC of the urinary bladder (14/30; 46.6%) prior to the detection of decoy cells in the urine. Nine of these UCC patients received intravesical treatment (BCG or mitomycin) after the first presentation with UCC. However, the clinical data on the treatment of the other five UCC patients was lacking. IHC identified BKPyV-positivity in the urine samples of non-UCC and UCC patients, while no BKPyV positivity was found in FFPE tissues of primary UCCs and metastases. In addition, BKPyV-PCR results revealed the presence of BKPyV DNA in the urine of the UCC cases, yet none in the UCC tissues itself. These data strongly indicate that BKPyV reactivation is not restricted to immunosuppression. It can be found in UCS of the immunocompetent patients and may be related to the intravesical BCG or mitomycin treatment of the UCC patients.

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