scholarly journals The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart

2021 ◽  
Vol 11 (17) ◽  
pp. 7768
Author(s):  
Xifeng Yang ◽  
Shihao Chen ◽  
Jiaxin Chen ◽  
Yunqi Liu ◽  
Ying Bai ◽  
...  
Keyword(s):  
Umbilical Vein ◽  
Protein Composition ◽  
Small Intestinal Submucosa ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Small Intestinal ◽  
Intestinal Submucosa ◽  
Umbilical Vein Endothelial Cells ◽  
Myocardial Injuries

Injectable decellularized matrix hydrogels derived from either myocardium or small intestinal submucosa (pDMYO-gel, pDSIS-gel) have been successfully used for myocardial injury repair. However, the relationship between tissue-specific biological functions and protein composition in these two materials is not clear yet. In this study, the protein composition, mechanical properties, and morphology of these two hydrogels and their effects on the behavior of neonatal rat cardiomyocytes (NRCMs) and human umbilical vein endothelial cells (HUVECs), are investigated. The results show that pDMYO-gel is more conducive to growth, adhesion, spreading, and maintenance of normal NRCM beating, due to its higher proportion of extracellular matrix (ECM) glycoproteins (49.55%) and some unique functional proteins such as annexin-6 (ANXA6), agrin (AGRN), cathepsin D (CTSD) and galectin-1 (LGALS1), whereas pDSIS-gel is more conducive to the proliferation of HUVECs. Animal study shows that pDMYO-gel has a better effect on improving cardiac function, inhibiting myocardial fibrosis and maintaining ventricular wall thickness in acute myocardial infarction models in vivo. Therefore, it is proposed that injectable pDMYO-gel hydrogel may be more suitable for functional recovery of myocardial injuries.

In vivo release of bovine serum albumin from an injectable small intestinal submucosa gel

2011 ◽  
Vol 420 (2) ◽  
pp. 266-273 ◽  
Author(s):  
Kkot Nim Kang ◽  
Da Yeon Kim ◽  
So Mi Yoon ◽  
Jin Seon Kwon ◽  
Hyo Won Seo ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Small Intestinal Submucosa ◽  
In Vivo Release ◽  
Small Intestinal ◽  
Intestinal Submucosa

scholarly journals Effect of Multilaminate Small Intestinal Submucosa as a Barrier Membrane on Bone Formation in a Rabbit Mandible Defect Model

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Weiyi Wu ◽  
Bowen Li ◽  
Yuhua Liu ◽  
Xinzhi Wang ◽  
Lin Tang
Keyword(s):  
Bone Regeneration ◽  
Porous Scaffold ◽  
Small Intestinal Submucosa ◽  
Control Group ◽  
Collagen Membrane ◽  
Defect Model ◽  
Barrier Membrane ◽  
Small Intestinal ◽  
Intestinal Submucosa

A barrier membrane (BM) is essential for guided bone regeneration (GBR) procedures. Absorbable BMs based on collagen have been widely applied clinically due to their excellent biocompatibility. The extracellular matrix (ECM) provides certain advantages that can compensate for the rapid degradation and insufficient mechanical strength of pure collagen membrane due to the porous scaffold structure. Recently, small intestinal submucosa (SIS), one of the most widely used ECM materials, has drawn much attention in bone tissue engineering. In this study, we adopted multilaminate SIS (mSIS) as a BM and evaluated its in vivo and in vitro properties. mSIS exhibited a multilaminate structure with a smooth upper surface and a significantly coarser bottom layer according to microscopic observation. Tensile strength was 13.10 ± 2.56 MPa. In in vivo experiments, we selected a rabbit mandibular defect model and subcutaneous implantation to compare osteogenesis and biodegradation properties with one of the most commonly used commercial collagen membranes. mSIS was retained for up to 3 months and demonstrated longer biodegradation time than commercial collagen membrane. Quantification of bone regeneration revealed significant differences in each group. Micro-computed tomography (micro-CT) revealed that the quantity and maturity of bones in the mSIS group were significantly higher than those in the blank control group (P < 0.05) and were similar to those in a commercial collagen membrane group (P > 0.05) at 4 and 12 weeks after surgery. Hematoxylin and eosin staining revealed large amounts of mature lamellar bone at 12 weeks in mSIS and commercial collagen membrane groups. Therefore, we conclude that mSIS has potential as a future biocompatible BM in GBR procedures.

IN VIVO TISSUE ENGINEERING OF THE COMMON BILE DUCT USING SMALL INTESTINAL SUBMUCOSA IN A CANINE MODEL

ASAIO Journal ◽  
2002 ◽  
Vol 48 (2) ◽  
pp. 197
Author(s):  
Dai Kimura ◽  
Tatsuo Nakamura ◽  
Kenji Kaino ◽  
Yoshio Hori ◽  
Masaki Nio ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bile Duct ◽  
Common Bile Duct ◽  
Canine Model ◽  
Small Intestinal Submucosa ◽  
In Vivo Tissue Engineering ◽  
The Common ◽  
Small Intestinal ◽  
Intestinal Submucosa

In Vivo Motility Evaluation of the Grafted Gastric Wall with Small Intestinal Submucosa

2010 ◽  
Vol 16 (5) ◽  
pp. 1761-1768 ◽  
Author(s):  
Taku Nishimura ◽  
Tomio Ueno ◽  
Hiroki Nakatsu ◽  
Atsunori Oga ◽  
Sei Kobayashi ◽  
...  
Keyword(s):  
Gastric Wall ◽  
Small Intestinal Submucosa ◽  
Small Intestinal ◽  
Intestinal Submucosa

scholarly journals Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier

Polymers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 247 ◽  
Author(s):  
Joon Park ◽  
Bo Song ◽  
Jin Lee ◽  
Seung Park ◽  
Tae Kang ◽  
...  
Keyword(s):  
Umbilical Vein ◽  
Hydrophobic Surface ◽  
Positive Control ◽  
Negative Control ◽  
In Vivo Evaluation ◽  
Acellular Matrix ◽  
Peritoneal Wall ◽  
Intestinal Submucosa ◽  
Umbilical Vein Endothelial Cells

In this paper, a cartilage acellular-matrix (CAM) is chosen as a biomaterial for an effective antiadhesive barrier to apply between injured tissue and healthy tissues or organs. CAM is cross-linked using glutaraldehyde to create a cross-linked CAM (Cx-CAM) film. Cx-CAM has higher elastic modulus and toughness and more hydrophobic surface properties than CAM before cross-linking. Small intestinal submucosa (SIS), cross-linked SIS (Cx-SIS) as a negative control, and Seprafilm as a positive control are used in an experiment as adhesion barriers. Human umbilical vein endothelial cells (HUVECs) on SIS, Cx-SIS, or in a culture plate get attached and effectively proliferate for 7 days, but Cx-CAM and Seprafilm allow for little or no attachment and proliferation of HUVECs, thus manifesting antiadhesive and antiproliferative effects. In animals with surgical damage to the peritoneal wall and cecum, Cx-CAM and Seprafilm afford little adhesion and negligible inflammation after seven days, as confirmed by hematoxylin and eosin staining and macrophage staining, in contrast to an untreated-injury model, SIS, or Cx-SIS film. Cx-CAM significantly suppresses the formation of blood vessels between the peritoneal wall and cecum, as confirmed by CD31 staining. Overall, the newly designed Cx-CAM film works well as an antiadhesion barrier and has better anti-tissue adhesion efficiency.

Study of biocompatibility of the material based on small intestinal submucosa in tests in vitro and in vivo

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
А.А. Dolgalev ◽  
А.А. Venediktov ◽  
D.V. Bobryshev ◽  
А.D. Kruchinina ◽  
А.А. Chagarov ◽  
...  
Keyword(s):  
Small Intestinal Submucosa ◽  
Small Intestinal ◽  
Intestinal Submucosa

circPTP4A2-miR-330-5p-PDK2 Signaling Facilitates In Vivo Survival of HuMSCs on SF-SIS Scaffolds and Improves The Repair of Damaged Endometrium

2021 ◽  
Author(s):  
Yuanyuan Zheng ◽  
Linhao Li ◽  
Xuewei Bi ◽  
Ruyue Xue
Keyword(s):  
Real Time ◽  
Small Intestinal Submucosa ◽  
Mitochondrial Metabolism ◽  
Human Umbilical Cord ◽  
Extracellular Flux ◽  
Cellular Oxygen ◽  
Small Intestinal ◽  
Intestinal Submucosa ◽  
The Impact

Abstract Background Human umbilical cord MSCs (HuMSC)-based therapy has shown promising results in the treatment of intrauterine adhesions (lUA). In this study, our aim was to construct a HuMSC-seeded silk fibroin small-intestinal submucosa (SF-SIS) scaffold and evaluate the impact of repairing the damaged endometrium in an lUA mouse model. Methods To identify the functional effect of HuMSCs-silk cellulose (SF)- small-intestinal submucosa (SIS) scaffolds on the repair of damaged endometrium, a mouse lUA model was established in this study. The uterine morphology and fibrosis were evaluated by hematoxylin - eosin (H&E) staining and Masson staining. CircRNA sequencing, real-time PCR and RNA fluorescence in situ hybridization were used to screen and verify the potential circRNAs that involved in the repair of damaged endometrium by HuMSCs. Real time integrated cellular oxygen consumption rate (OCR) was measured using the Seahorse XF24 Extracellular Flux Analyser. The potential down-stream miRNAs and proteins of circRNAs were analyzed dual-luciferase report and Western Blot. Results We found that HuMSCs-SF-SIS not only increased the number of glands, but also reduced the ulcer area in the IUA model. Furthermore, we demonstrated that circPTP4A2 was elevated in the HuMSCs seeded on the SF-SIS scaffolds and stabilized the mitochondrial metabolism through miR-330-5p-PDK2 signaling, which contributes to endometrial repair progression. Conclusion In this study, we demonstrated that circPTP4A2 was elevated in the HuMSCs seeded on the SF-SIS scaffolds and stabilized the mitochondrial metabolism through miR-330-5p-PDK2 signaling, which contributes to endometrial repair progression. These findings demonstrate that HuMSC-seeded SF-SIS scaffolds are an encouraging method for the treatment of lUA.

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