Bioengineering Organs Using Small Intestinal Submucosa Scaffolds: In Vivo Tissue-Engineering Technology

2000 ◽  
Vol 14 (1) ◽  
pp. 59-62 ◽  
Author(s):  
BRADLEY P. KROPP ◽  
EARL Y. CHENG
Keyword(s):  
Tissue Engineering ◽  
Small Intestinal Submucosa ◽  
Engineering Technology ◽  
In Vivo Tissue Engineering ◽  
Small Intestinal ◽  
Intestinal Submucosa ◽  
Tissue Engineering Technology

IN VIVO TISSUE ENGINEERING OF THE COMMON BILE DUCT USING SMALL INTESTINAL SUBMUCOSA IN A CANINE MODEL

ASAIO Journal ◽  
2002 ◽  
Vol 48 (2) ◽  
pp. 197
Author(s):  
Dai Kimura ◽  
Tatsuo Nakamura ◽  
Kenji Kaino ◽  
Yoshio Hori ◽  
Masaki Nio ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bile Duct ◽  
Common Bile Duct ◽  
Canine Model ◽  
Small Intestinal Submucosa ◽  
In Vivo Tissue Engineering ◽  
The Common ◽  
Small Intestinal ◽  
Intestinal Submucosa

Biomimetic porous scaffolds containing decellularized small intestinal submucosa and Sr2+/Fe3+ co-doped hydroxyapatite accelerate angiogenesis/osteogenesis for bone regeneration

2022 ◽  
Author(s):  
Wei Cui ◽  
Liang Yang ◽  
Ismat Ullah ◽  
Keda Yu ◽  
Zhigang Zhao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Small Intestinal Submucosa ◽  
Bone Scaffolds ◽  
Small Intestinal ◽  
Intestinal Submucosa ◽  
Co Doped

Abstract The design of bone scaffolds is predominately aimed to well reproduce the natural bony environment by imitating the architecture/composition of host bone. Such biomimetic biomaterials are gaining increasing attention and acknowledged quite promising for bone tissue engineering. Herein, novel biomimetic bone scaffolds containing decellularized small intestinal submucosa matrix (SIS-ECM) and Sr2+/Fe3+ co-doped hydroxyapatite (SrFeHA) are fabricated for the first time by the sophisticated self-assembled mineralization procedure, followed by cross-linking and lyophilization post-treatments. The results indicate the constructed SIS/SrFeHA scaffolds are characterized by highly porous structures, rough microsurface and improved mechanical strength, as well as efficient releasing of bioactive Sr2+/Fe3+ and ECM components. These favorable physico-chemical properties endow SIS/SrFeHA scaffolds with an architectural/componential biomimetic bony environment which appears to be highly beneficial for inducing angiogenesis/osteogenesis both in vitro and in vivo. In particular, the cellular functionality and bioactivity of endotheliocytes/osteoblasts are significantly enhanced by SIS/SrFeHA scaffolds, and the cranial defects model further verifies the potent ability of SIS/SrFeHA to accelerate in vivo vascularization and bone regeneration following implantation. In this view these results highlight the considerable angiogenesis/osteogenesis potential of biomimetic porous SIS/SrFeHA scaffolds for inducing bone regeneration and thus may afford a new promising alternative for bone tissue engineering.

Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (5) ◽  
pp. 1317-1326 ◽  
Author(s):  
Shaofeng Wu ◽  
Yan Liu ◽  
Shantaram Bharadwaj ◽  
Anthony Atala ◽  
Yuanyuan Zhang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Human Urine ◽  
Small Intestinal Submucosa ◽  
Small Intestinal ◽  
Intestinal Submucosa

DEVELOPMENTAL STATUS AND PERSPECTIVES FOR TISSUE ENGINEERING IN UROLOGY

2021 ◽  
Vol 12 (07) ◽  
pp. 5-13
Author(s):  
Elcin Nizami Huseyn ◽  
Keyword(s):  
Tissue Engineering ◽  
Biological Materials ◽  
Organ Damage ◽  
Biological Tissues ◽  
Tissue Cell ◽  
Sperm Cells ◽  
Engineering Technology ◽  
The Future ◽  
Tissue Engineering Technology

Tissue engineering technology and tissue cell-based stem cell research have made great strides in treating tissue and organ damage, correcting tissue and organ dysfunction, and reducing surgical complications. In the past, traditional methods have used biological substitutes for tissue repair materials, while tissue engineering technology has focused on merging sperm cells with biological materials to form biological tissues with the same structure and function as their own tissues. The advantage is that tissue engineering technology can overcome donors. Material procurement restrictions can effectively reduce complications. The aim of studying tissue engineering technology is to find sperm cells and suitable biological materials to replace the original biological functions of tissues and to establish a suitable in vivo microenvironment. This article mainly describes the current developments of tissue engineering in various fields of urology and discusses the future trends of tissue engineering technology in the treatment of complex diseases of the urinary system. The results of the research in this article indicate that while the current clinical studies are relatively few, the good results from existing animal model studies indicate good prospects of tissue engineering technology for the treatment of various urinary tract diseases in the future. Key words: Tissue engineering, kidney, ureter, bladder, urethra

scholarly journals A Bibliometric Review of Artificial Extracellular Matrices Based on Tissue Engineering Technology Literature: 1990 through 2019

Materials ◽  
2020 ◽  
Vol 13 (13) ◽  
pp. 2891
Author(s):  
Pilar Simmons ◽  
Taylor McElroy ◽  
Antiño R. Allen
Keyword(s):  
Tissue Engineering ◽  
Therapeutic Option ◽  
Extracellular Matrices ◽  
Engineering Technology ◽  
Network Analyses ◽  
Bibliometric Review ◽  
Artificial Extracellular Matrix ◽  
Tissue Engineering Technology

Artificial extracellular matrices (aECMs) are an extension of biomaterials that were developed as in-vitro model environments for tissue cells that mimic the native in vivo target tissues’ structure. This bibliometric analysis evaluated the research productivity regarding aECM based on tissue engineering technology. The Web of Science citation index was examined for articles published from 1990 through 2019 using three distinct aECM-related topic sets. Data were also visualized using network analyses (VOSviewer). Terms related to in-vitro, scaffolds, collagen, hydrogels, and differentiation were reoccurring in the aECM-related literature over time. Publications with terms related to a clinical direction (wound healing, stem cells, artificial skin, in-vivo, and bone regeneration) have steadily increased, as have the number of countries and institutions involved in the artificial extracellular matrix. As progress with 3D scaffolds continues to advance, it will become the most promising technology to provide a therapeutic option to repair or replace damaged tissue.

In vivo release of bovine serum albumin from an injectable small intestinal submucosa gel

2011 ◽  
Vol 420 (2) ◽  
pp. 266-273 ◽  
Author(s):  
Kkot Nim Kang ◽  
Da Yeon Kim ◽  
So Mi Yoon ◽  
Jin Seon Kwon ◽  
Hyo Won Seo ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Small Intestinal Submucosa ◽  
In Vivo Release ◽  
Small Intestinal ◽  
Intestinal Submucosa

scholarly journals Effect of Multilaminate Small Intestinal Submucosa as a Barrier Membrane on Bone Formation in a Rabbit Mandible Defect Model

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Weiyi Wu ◽  
Bowen Li ◽  
Yuhua Liu ◽  
Xinzhi Wang ◽  
Lin Tang
Keyword(s):  
Bone Regeneration ◽  
Porous Scaffold ◽  
Small Intestinal Submucosa ◽  
Control Group ◽  
Collagen Membrane ◽  
Defect Model ◽  
Barrier Membrane ◽  
Small Intestinal ◽  
Intestinal Submucosa

A barrier membrane (BM) is essential for guided bone regeneration (GBR) procedures. Absorbable BMs based on collagen have been widely applied clinically due to their excellent biocompatibility. The extracellular matrix (ECM) provides certain advantages that can compensate for the rapid degradation and insufficient mechanical strength of pure collagen membrane due to the porous scaffold structure. Recently, small intestinal submucosa (SIS), one of the most widely used ECM materials, has drawn much attention in bone tissue engineering. In this study, we adopted multilaminate SIS (mSIS) as a BM and evaluated its in vivo and in vitro properties. mSIS exhibited a multilaminate structure with a smooth upper surface and a significantly coarser bottom layer according to microscopic observation. Tensile strength was 13.10 ± 2.56 MPa. In in vivo experiments, we selected a rabbit mandibular defect model and subcutaneous implantation to compare osteogenesis and biodegradation properties with one of the most commonly used commercial collagen membranes. mSIS was retained for up to 3 months and demonstrated longer biodegradation time than commercial collagen membrane. Quantification of bone regeneration revealed significant differences in each group. Micro-computed tomography (micro-CT) revealed that the quantity and maturity of bones in the mSIS group were significantly higher than those in the blank control group (P < 0.05) and were similar to those in a commercial collagen membrane group (P > 0.05) at 4 and 12 weeks after surgery. Hematoxylin and eosin staining revealed large amounts of mature lamellar bone at 12 weeks in mSIS and commercial collagen membrane groups. Therefore, we conclude that mSIS has potential as a future biocompatible BM in GBR procedures.

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