scholarly journals Fluorescence Correlation Spectroscopy Measurement Based on Fiber Optics for Biological Materials

2021 ◽  
Vol 11 (15) ◽  
pp. 6744
Author(s):  
Johtaro Yamamoto ◽  
Akira Sasaki
Keyword(s):  
Fiber Optics ◽  
Fluorescence Correlation Spectroscopy ◽  
Fiber Optic ◽  
Fluorescent Dyes ◽  
Water Immersion ◽  
Correlation Spectroscopy ◽  
Objective Lens ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics ◽  
Fluorescent Molecules

A robust fluorescence correlation spectroscopy system called fiber-optic based fluorescence correlation spectroscopy (FB-FCS) was developed; this system enables the measurement of diffusion dynamics and concentration of fluorescent molecules based on the principle of fluorescence correlation spectroscopy without any mechanical adjustment of the experimental setup. The system consisted of fiber optics and a water-immersion objective lens. The hydrodynamic diameters and concentrations of organic fluorescent dyes and fluorescently labeled proteins were successfully measured. Because of the fiber-optic-based setup, the FB-FCS system is compact and inexpensive. We expect FB-FCS to be suitable for use in laboratories, medical diagnosis, and environmental measurements.

scholarly journals Advanced processing and analysis of conventional confocal microscopy generated scanning FCS data

2017 ◽  
Author(s):  
Dominic Waithe ◽  
Falk Schneider ◽  
Jakub Chojnacki ◽  
Mathias Clausen ◽  
Dilip Shrestha ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Spatial Heterogeneity ◽  
Fluorescence Correlation Spectroscopy ◽  
Large Scale ◽  
Molecular Diffusion ◽  
Correlation Spectroscopy ◽  
The Other ◽  
Statistical Accuracy ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics

AbstractScanning Fluorescence Correlation Spectroscopy (scanning FCS) is a variant of conventional point FCS that allows molecular diffusion at multiple locations to be measured simultaneously. It enables disclosure of potential spatial heterogeneity in molecular diffusion dynamics and also the acquisition of a large amount of FCS data at the same time, providing large statistical accuracy. Here, we optimize the processing and analysis of these large-scale acquired sets of FCS data. On one hand we present FoCuS-scan, scanning FCS software that provides an end-to-end solution for processing and analysing scanning data acquired on commercial turnkey confocal systems. On the other hand, we provide a thorough characterisation of large-scale scanning FCS data over its intended time-scales and applications and propose a unique solution for the bias and variance observed when studying slowly diffusing species. Our manuscript enables researchers to straightforwardly utilise scanning FCS as a powerful technique for measuring diffusion across a broad range of physiologically relevant length scales without specialised hardware or expensive software.

scholarly journals Diffusion of fluorescent molecules and quantum dot nanoparticles in viscous solvents investigated by fluorescence correlation spectroscopy

2018 ◽  
Vol 56 (4) ◽  
pp. 488-492
Author(s):  
Nguyen Thi Thanh Bao ◽  
Dinh Van Trung ◽  
Nguyen Thanh Binh ◽  
Vu Thi Bich ◽  
Ung Thi Dieu Thuy ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Viscous Solvents ◽  
Fluorescent Molecules

scholarly journals Interferometric Fluorescent Cross Correlation Spectroscopy

2019 ◽  
Author(s):  
Ipsita Saha ◽  
Saveez Saffarian
Keyword(s):  
Quantum Dots ◽  
Fluorescence Correlation Spectroscopy ◽  
Cross Correlation ◽  
Single Photon ◽  
Theoretical Framework ◽  
Correlation Spectroscopy ◽  
Aqueous Samples ◽  
Cellular Membranes ◽  
Fluorescence Correlation ◽  
Fluorescent Molecules

AbstractWe present a method utilizing single photon interference and fluorescence correlation spectroscopy (FCS) to simultaneously measure transport of fluorescent molecules within aqueous samples. Our method, within seconds, measures transport in thousands of homogenous voxels (100 nm)3 and eliminates photo-physical artifacts associated with blinking of fluorescent molecules. A comprehensive theoretical framework is presented and validated by measuring transport of quantum dots, associated with VSV-G receptor along cellular membranes as well as within viscous gels.

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