Probing the Role of Chain Length on the Diffusion Dynamics of π-Conjugated Polymers by Fluorescence Correlation Spectroscopy

2011 ◽  
Vol 115 (37) ◽  
pp. 10779-10788 ◽  
Author(s):  
A. V. R. Murthy ◽  
Mahima Goel ◽  
Shivprasad Patil ◽  
M. Jayakannan
Keyword(s):  
Conjugated Polymers ◽  
Chain Length ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics

CHARACTERIZATION OF POLY(ACRYLIC ACID) DIFFUSION DYNAMICS ON THE GRAFTED SURFACE OF POLY(ETHYLENE TEREPHTHALATE) FILMS BY FLUORESCENCE CORRELATION SPECTROSCOPY

2006 ◽  
Vol 01 (04) ◽  
pp. 433-441 ◽  
Author(s):  
XIAOTAO PAN ◽  
CAILI AW ◽  
YANAN DU ◽  
HANRY YU ◽  
THORSTEN WOHLAND
Keyword(s):  
Ionic Strength ◽  
Acrylic Acid ◽  
Chain Length ◽  
Fluorescence Correlation Spectroscopy ◽  
Ethylene Terephthalate ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Poly Ethylene Terephthalate ◽  
Diffusion Dynamics ◽  
Poly Ethylene

Poly(acrylic acid) (PAAc) is a commonly used polymer grafted on poly(ethylene terephthalate) films for the immobilization of bioactive molecules that directly interact with living cells or tissues for the maintenance of their viability and functionality. The diffusion property of the grafted PAAc on the surface is a critical parameter related to the density, length of polymer chains, and ionic strength of the solution. Fluorescence correlation spectroscopy (FCS) is able to measure the diffusion coefficient of fluorescent particles in solution with single molecule sensitivity and specificity. It was used as an effective tool to detect diffusion dynamics of Atto 565 molecules, a good indicator for viscosity of PAAc, in both aqueous polymer solutions and polymer grafted film surfaces immersed in solution. In this work we determine the polymer chain length under different polymerization conditions in solution and deduce the solution viscosity by FCS measurements of Atto 565 as tracer molecule. By using the same tracer on the grafted polymer films we can infer the viscosity of these grafted layers under a variety of conditions, including the PAAc chain length, the UV exposure time during polymerization, the ionic strength, and the pH value of the immersed solution.

scholarly journals Advanced processing and analysis of conventional confocal microscopy generated scanning FCS data

2017 ◽  
Author(s):  
Dominic Waithe ◽  
Falk Schneider ◽  
Jakub Chojnacki ◽  
Mathias Clausen ◽  
Dilip Shrestha ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Spatial Heterogeneity ◽  
Fluorescence Correlation Spectroscopy ◽  
Large Scale ◽  
Molecular Diffusion ◽  
Correlation Spectroscopy ◽  
The Other ◽  
Statistical Accuracy ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics

AbstractScanning Fluorescence Correlation Spectroscopy (scanning FCS) is a variant of conventional point FCS that allows molecular diffusion at multiple locations to be measured simultaneously. It enables disclosure of potential spatial heterogeneity in molecular diffusion dynamics and also the acquisition of a large amount of FCS data at the same time, providing large statistical accuracy. Here, we optimize the processing and analysis of these large-scale acquired sets of FCS data. On one hand we present FoCuS-scan, scanning FCS software that provides an end-to-end solution for processing and analysing scanning data acquired on commercial turnkey confocal systems. On the other hand, we provide a thorough characterisation of large-scale scanning FCS data over its intended time-scales and applications and propose a unique solution for the bias and variance observed when studying slowly diffusing species. Our manuscript enables researchers to straightforwardly utilise scanning FCS as a powerful technique for measuring diffusion across a broad range of physiologically relevant length scales without specialised hardware or expensive software.

scholarly journals Characterizing diffusion dynamics of a membrane protein associated with nanolipoproteins using fluorescence correlation spectroscopy

2011 ◽  
Vol 20 (2) ◽  
pp. 437-447 ◽  
Author(s):  
Tingjuan Gao ◽  
Craig D. Blanchette ◽  
Wei He ◽  
Feliza Bourguet ◽  
Sonny Ly ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics

Diffusion and aggregation in biological membranes

1984 ◽  
Vol 62 (11) ◽  
pp. 1158-1166 ◽  
Author(s):  
Nils O. Petersen
Keyword(s):  
Membrane Proteins ◽  
Fluorescence Correlation Spectroscopy ◽  
Membrane Lipids ◽  
Living Cells ◽  
Correlation Spectroscopy ◽  
New Approach ◽  
Fluorescence Correlation ◽  
Quantitative Measurements ◽  
Membrane Components

Membrane lipids and proteins diffuse laterally in the membrane plane unless constrained by interactions with immobile or slowly moving components. Direct diffusion measurements help characterize the interactions among surface components and can provide information about the mechanism of physiologically interesting processes in membranes. Association of membrane proteins into aggregates may result from changes in intermolecular interactions and will affect the rate of diffusion of the protein. Quantitative measurements of aggregation are therefore becoming important both to aid interpretation of constraints of diffusion and to explore the role of aggregation in physiological events. In this paper, the popular fluorescence photobleaching measurements of diffusion are discussed and their relevance is illustrated with selected examples. Moreover, a variation on the theme of fluorescence correlation spectroscopy is presented as a new approach to measuring the state of aggregation of membrane components on surfaces of living cells

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