scholarly journals Probing transcription factor diffusion dynamics in the living mammalian embryo with photoactivatable fluorescence correlation spectroscopy

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Gurpreet Kaur ◽  
Mauro W. Costa ◽  
Christian M. Nefzger ◽  
Juan Silva ◽  
Juan Carlos Fierro-González ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Mammalian Embryo ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics

scholarly journals Advanced processing and analysis of conventional confocal microscopy generated scanning FCS data

2017 ◽  
Author(s):  
Dominic Waithe ◽  
Falk Schneider ◽  
Jakub Chojnacki ◽  
Mathias Clausen ◽  
Dilip Shrestha ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Spatial Heterogeneity ◽  
Fluorescence Correlation Spectroscopy ◽  
Large Scale ◽  
Molecular Diffusion ◽  
Correlation Spectroscopy ◽  
The Other ◽  
Statistical Accuracy ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics

AbstractScanning Fluorescence Correlation Spectroscopy (scanning FCS) is a variant of conventional point FCS that allows molecular diffusion at multiple locations to be measured simultaneously. It enables disclosure of potential spatial heterogeneity in molecular diffusion dynamics and also the acquisition of a large amount of FCS data at the same time, providing large statistical accuracy. Here, we optimize the processing and analysis of these large-scale acquired sets of FCS data. On one hand we present FoCuS-scan, scanning FCS software that provides an end-to-end solution for processing and analysing scanning data acquired on commercial turnkey confocal systems. On the other hand, we provide a thorough characterisation of large-scale scanning FCS data over its intended time-scales and applications and propose a unique solution for the bias and variance observed when studying slowly diffusing species. Our manuscript enables researchers to straightforwardly utilise scanning FCS as a powerful technique for measuring diffusion across a broad range of physiologically relevant length scales without specialised hardware or expensive software.

scholarly journals In vivo fluorescence correlation spectroscopy analyses of FMBP ‐1, a silkworm transcription factor

FEBS Open Bio ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 106-125 ◽  
Author(s):  
Motosuke Tsutsumi ◽  
Hideki Muto ◽  
Shohei Myoba ◽  
Mai Kimoto ◽  
Akira Kitamura ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
In Vivo Fluorescence

scholarly journals Monitoring the interaction between DNA and a transcription factor (MEF2A) using fluorescence correlation spectroscopy

2005 ◽  
Vol 328 (12) ◽  
pp. 1033-1040 ◽  
Author(s):  
Guillaume Octobre ◽  
Claudie Lemercier ◽  
Saadi Khochbin ◽  
Michel Robert-Nicoud ◽  
Catherine Souchier
Keyword(s):  
Transcription Factor ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation
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