scholarly journals Incorporation of Bioactive Glasses Containing Mg, Sr, and Zn in Electrospun PCL Fibers by Using Benign Solvents

2020 ◽  
Vol 10 (16) ◽  
pp. 5530 ◽  
Author(s):  
Rachele Sergi ◽  
Valeria Cannillo ◽  
Aldo R. Boccaccini ◽  
Liliana Liverani
Keyword(s):  
Cell Adhesion ◽  
Bioactive Glass ◽  
Biomedical Application ◽  
Electrospun Fiber ◽  
Glass Composite ◽  
Bioactive Glasses ◽  
Electrospinning Technique ◽  
Fiber Mats ◽  
Cell Adhesion And Proliferation

Poly(ε-caprolactone) (PCL) and PCL/bioactive glass composite fiber mats were produced by electrospinning technique. To improve cell adhesion and proliferation (i) 45S5, (ii) a bioactive glass containing strontium and magnesium oxides, and (iii) a bioactive glass containing zinc oxide were separately added to the starting PCL solution before electrospinning. A good incorporation of bioactive glass particles in PCL electrospun mats was confirmed by SEM and FTIR analyses. Bioactivity was evaluated by immersion of PCL mats and PCL/bioactive glass electrospun fiber mats in simulated body fluid (SBF). Bone murine stromal cells (ST-2) were employed in WST-8 assay to assess cell viability, cell morphology, and proliferation. The results showed that the presence of bioactive glass particles in the fibers enhances cell adhesion and proliferation compared to neat PCL mats. Furthermore, PCL/bioactive glass electrospun mats showed higher wound-healing rate (measured as cell migration rate) in vitro compared to neat PCL electrospun mats. Therefore, the characteristics of the PCL matrix combined with biological properties of bioactive glasses make PCL/bioactive glass composite ideal candidate for biomedical application.

scholarly journals Bioactive Glass Nanoparticles-Loaded Poly(ɛ-caprolactone) Nanofiber as Substrate for ARPE-19 Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Tadeu Henrique Lima ◽  
Gabriella Maria Fernandes-Cunha ◽  
Carlos Eduardo de Matos Jensen ◽  
Rodrigo Lambert Oréfice ◽  
Armando da Silva-Cunha Junior ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Bioactive Glass ◽  
Protein Adsorption ◽  
Chemical Structure ◽  
Chorioallantoic Membrane ◽  
Proliferative Capacity ◽  
Sem Images ◽  
Cell Substrate ◽  
Cell Adhesion And Proliferation

Bioactive glass nanoparticles-loaded poly(ɛ-caprolactone) nanofibers (BIOG PCL nanofibers) were synthesized and evaluated as substrates for ocular cells (ARPE-19). BIOG PCL nanofibers were characterized using SEM, FTIR, and DSC, and thein vitrodegradation profile was also investigated. Thein vitroocular biocompatibility of nanofibers was exploited in Müller glial cells (MIO-M1 cells) and in chorioallantoic membrane (CAM); and the proliferative capacity, cytotoxicity, and functionality were evaluated. Finally, ARPE-19 cells were seeded onto BIOG PCL nanofibers and they were investigated as supports forin vitrocell adhesion and proliferation. SEM images revealed the incorporation of BIOG nanoparticles into PCL nanofibers. Nanoparticles did not induce modifications in the chemical structure and semicrystalline nature of PCL in the nanofiber, as shown by FTIR and DSC. MIO-M1 cells exposed to BIOG PCL nanofibers showed viability, and they were able to proliferate and to express GFAP, indicating cellular functionality. Moreover, nanofibers were well tolerated by CAM. These findings suggested thein vitroocular biocompatibility and absence of toxicity of these nanofibers. Finally, the BIOG nanoparticles modulated the protein adsorption, and, subsequently, ARPE-19 cells adhered and proliferated onto the nanostructured supports, establishing cell-substrate interactions. In conclusion, the biodegradable and biocompatible BIOG PCL nanofibers supported the ARPE-19 cells.

scholarly journals Chitosan-Based Bioactive Glass Gauze: Microstructural Properties, In Vitro Bioactivity, and Biological Tests

Materials ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 2819 ◽  
Author(s):  
Rachele Sergi ◽  
Devis Bellucci ◽  
Roberta Salvatori ◽  
Valeria Cannillo
Keyword(s):  
Wound Healing ◽  
Cell Adhesion ◽  
Bioactive Glass ◽  
Organic Matrix ◽  
Scratch Test ◽  
Neutral Red ◽  
Wound Dressings ◽  
Nih 3T3 ◽  
Cell Adhesion And Proliferation

Passive commercial gauzes were turned into interactive wound dressings by impregnating them with a chitosan suspension. To further improve healing, and cell adhesion and proliferation, chitosan/bioactive glass wound dressings were produced with the addition of (i) 45S5, (ii) a Sr- and Mg-containing bioactive glass, and (iii) a Zn-containing bioactive glass to the chitosan suspension. SEM and FTIR analyses evidenced positive results in terms of incorporation of bioactive glass particles. Bioactivity was investigated by soaking chitosan-based bioactive glass wound dressings in simulated body fluid (SBF). Cell viability, proliferation, and morphology were investigated using NIH 3T3 (mouse embryonic fibroblast) cells by neutral red (NR) uptake and MTT assays. Furthermore, the wound-healing rate was evaluated by means of the scratch test, using NIH 3T3. The results showed that bioactive glass particles enhance cell adhesion and proliferation, and wound healing compared to pure chitosan. Therefore, chitosan-based bioactive glass wound dressings combine the properties of the organic matrix with the specific biological characteristics of bioactive glasses to achieve chitosan composites suitable for healing devices.

scholarly journals Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization

2018 ◽  
Vol 9 (4) ◽  
pp. 62 ◽  
Author(s):  
Gianluca Turco ◽  
Davide Porrelli ◽  
Eleonora Marsich ◽  
Federica Vecchies ◽  
Teresa Lombardi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Clinical Performance ◽  
Maxillofacial Surgery ◽  
Chemical Characterization ◽  
Bone Substitutes ◽  
Human Cell Line ◽  
Structural Features ◽  
Oral And Maxillofacial Surgery ◽  
Cell Adhesion And Proliferation

Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials.

scholarly journals A New Generation of Electrospun Fibers Containing Bioactive Glass Particles for Wound Healing

Materials ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 5651
Author(s):  
Rachele Sergi ◽  
Valeria Cannillo ◽  
Aldo R. Boccaccini ◽  
Liliana Liverani
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Bioactive Glass ◽  
Flexible Structures ◽  
Biological Properties ◽  
Cellular Viability ◽  
Bioactive Glasses ◽  
Electrospinning Technique ◽  
Potential Applications ◽  
Biological Performance

Chitosan fibers blended with polyethylene oxide (CHIT_PEO) and crosslinked with genipin were fabricated by electrospinning technique. Subsequently, CHIT_PEO bioactive glass composite electrospun mats were fabricated with the aim to achieve flexible structures with adequate mechanical properties and improved biological performance respect to CHIT_PEO fibers, for potential applications in wound healing. Three different compositions of bioactive glasses (BG) were selected and investigated: 45S5 BG, a Sr and Mg containing bioactive glass (BGMS10) and a Zn-containing bioactive glass (BGMS_2Zn). Particulate BGs (particles size < 20 μm) were separately added to the starting CHIT_PEO solution before electrospinning. The two recently developed bioactive glasses (BGMS10 and BGMS_2Zn) showed very promising biological properties in terms of bioactivity and cellular viability; thus, such compositions were added for the first time to CHIT_PEO solution to fabricate composite electrospun mats. The incorporation of bioactive glass particles and their distribution into CHIT_PEO fibers were assessed by SEM and FTIR analyses. Furthermore, CHIT_PEO composite electrospun mats showed improved mechanical properties in terms of Young’s Modulus compared to neat CHIT_PEO fibers; on the contrary, the values of tensile strain at break (%) were comparable. Biological performance in terms of cellular viability was investigated by means of WST-8 assay and CHIT_PEO composite electrospun mats showed cytocompatibility and the desired cellular viability.

scholarly journals Hierarchical Structures and Shaped Particles of Bioactive Glass and ItsIn VitroBioactivity

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
U. Boonyang ◽  
F. Li ◽  
A. Stein
Keyword(s):  
Body Temperature ◽  
Simulated Body Fluid ◽  
Bioactive Glass ◽  
Body Fluid ◽  
Colloidal Crystals ◽  
Hierarchical Structures ◽  
The Other ◽  
Bioactive Glasses ◽  
Ordered Macroporous

In this study, bioactive glass particles with controllable structure and porosity were prepared using dual-templating methods. Block copolymers used as one template component produced mesopores in the calcined samples. Polymer colloidal crystals as the other template component yielded either three-dimensionally ordered macroporous (3DOM) products or shaped bioactive glass nanoparticles. Thein vitrobioactivity of these bioactive glasses was studied by soaking the samples in simulated body fluid (SBF) at body temperature (37°C) for varying lengths of time and monitoring the formation of bone-like apatite on the surface of the bioactive glass. A considerable bioactivity was found that all of bioactive glass samples have the ability to induce the formation of an apatite layer on its surface when in contact with SBF. The development of bone-like apatite is faster for 3DOM bioactive glasses than for nanoparticles.

scholarly journals Effect of Melt-Derived Bioactive Glass Particles on the Properties of Chitosan Scaffolds

2019 ◽  
Vol 10 (3) ◽  
pp. 38 ◽  
Author(s):  
Hamasa Faqhiri ◽  
Markus Hannula ◽  
Minna Kellomäki ◽  
Maria Teresa Calejo ◽  
Jonathan Massera
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bioactive Glass ◽  
Pore Size ◽  
Glass Content ◽  
Micro Computed Tomography ◽  
Glass Composite ◽  
Composite Scaffolds ◽  
Bioactive Glasses

This study reports on the processing of three-dimensional (3D) chitosan/bioactive glass composite scaffolds. On the one hand, chitosan, as a natural polymer, has suitable properties for tissue engineering applications but lacks bioactivity. On the other hand, bioactive glasses are known to be bioactive and to promote a higher level of bone formation than any other biomaterial type. However, bioactive glasses are hard, brittle, and cannot be shaped easily. Therefore, in the past years, researchers have focused on the processing of new composites. Difficulties in reaching composite materials made of polymer (synthetic or natural) and bioactive glass include: (i) The high glass density, often resulting in glass segregation, and (ii) the fast bioactive glass reaction when exposed to moisture, leading to changes in the glass reactivity and/or change in the polymeric matrix. Samples were prepared with 5, 15, and 30 wt% of bioactive glass S53P4 (BonAlive ®), as confirmed using thermogravimetric analysis. MicrO–Computed tomography and optical microscopy revealed a flaky structure with porosity over 80%. The pore size decreased when increasing the glass content up to 15 wt%, but increased back when the glass content was 30 wt%. Similarly, the mechanical properties (in compression) of the scaffolds increased for glass content up to 15%, but decreased at higher loading. Ions released from the scaffolds were found to lead to precipitation of a calcium phosphate reactive layer at the scaffold surface. This is a first indication of the potential bioactivity of these materials. Overall, chitosan/bioactive glass composite scaffolds were successfully produced with pore size, machinability, and ability to promote a calcium phosphate layer, showing promise for bone tissue engineering and the mechanical properties can justify their use in non-load bearing applications.

In vitro corrosion and cytocompatibility studies of hot press sintered magnesium-bioactive glass composite

Materialia ◽  
2019 ◽  
Vol 5 ◽  
pp. 100245 ◽  
Author(s):  
Sourav Dutta ◽  
K. Bavya Devi ◽  
Santanu Mandal ◽  
Arnab Mahato ◽  
Sanjay Gupta ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Hot Press ◽  
Glass Composite

In vitro degradation behaviors of Poly-l-lactide/bioactive glass composite materials in phosphate-buffered solution

2009 ◽  
Vol 63 (4) ◽  
pp. 575-586 ◽  
Author(s):  
Zhihua Zhou ◽  
Qingfeng Yi ◽  
Xiaoping Liu ◽  
Lihua Liu ◽  
Qingquan Liu
Keyword(s):  
Composite Materials ◽  
Bioactive Glass ◽  
Glass Composite ◽  
In Vitro Degradation ◽  
Degradation Behaviors
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