scholarly journals Bioactive Glass Nanoparticles-Loaded Poly(ɛ-caprolactone) Nanofiber as Substrate for ARPE-19 Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Tadeu Henrique Lima ◽  
Gabriella Maria Fernandes-Cunha ◽  
Carlos Eduardo de Matos Jensen ◽  
Rodrigo Lambert Oréfice ◽  
Armando da Silva-Cunha Junior ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Bioactive Glass ◽  
Protein Adsorption ◽  
Chemical Structure ◽  
Chorioallantoic Membrane ◽  
Proliferative Capacity ◽  
Sem Images ◽  
Cell Substrate ◽  
Cell Adhesion And Proliferation

Bioactive glass nanoparticles-loaded poly(ɛ-caprolactone) nanofibers (BIOG PCL nanofibers) were synthesized and evaluated as substrates for ocular cells (ARPE-19). BIOG PCL nanofibers were characterized using SEM, FTIR, and DSC, and thein vitrodegradation profile was also investigated. Thein vitroocular biocompatibility of nanofibers was exploited in Müller glial cells (MIO-M1 cells) and in chorioallantoic membrane (CAM); and the proliferative capacity, cytotoxicity, and functionality were evaluated. Finally, ARPE-19 cells were seeded onto BIOG PCL nanofibers and they were investigated as supports forin vitrocell adhesion and proliferation. SEM images revealed the incorporation of BIOG nanoparticles into PCL nanofibers. Nanoparticles did not induce modifications in the chemical structure and semicrystalline nature of PCL in the nanofiber, as shown by FTIR and DSC. MIO-M1 cells exposed to BIOG PCL nanofibers showed viability, and they were able to proliferate and to express GFAP, indicating cellular functionality. Moreover, nanofibers were well tolerated by CAM. These findings suggested thein vitroocular biocompatibility and absence of toxicity of these nanofibers. Finally, the BIOG nanoparticles modulated the protein adsorption, and, subsequently, ARPE-19 cells adhered and proliferated onto the nanostructured supports, establishing cell-substrate interactions. In conclusion, the biodegradable and biocompatible BIOG PCL nanofibers supported the ARPE-19 cells.

scholarly journals Incorporation of Bioactive Glasses Containing Mg, Sr, and Zn in Electrospun PCL Fibers by Using Benign Solvents

2020 ◽  
Vol 10 (16) ◽  
pp. 5530 ◽  
Author(s):  
Rachele Sergi ◽  
Valeria Cannillo ◽  
Aldo R. Boccaccini ◽  
Liliana Liverani
Keyword(s):  
Cell Adhesion ◽  
Bioactive Glass ◽  
Biomedical Application ◽  
Electrospun Fiber ◽  
Glass Composite ◽  
Bioactive Glasses ◽  
Electrospinning Technique ◽  
Fiber Mats ◽  
Cell Adhesion And Proliferation

Poly(ε-caprolactone) (PCL) and PCL/bioactive glass composite fiber mats were produced by electrospinning technique. To improve cell adhesion and proliferation (i) 45S5, (ii) a bioactive glass containing strontium and magnesium oxides, and (iii) a bioactive glass containing zinc oxide were separately added to the starting PCL solution before electrospinning. A good incorporation of bioactive glass particles in PCL electrospun mats was confirmed by SEM and FTIR analyses. Bioactivity was evaluated by immersion of PCL mats and PCL/bioactive glass electrospun fiber mats in simulated body fluid (SBF). Bone murine stromal cells (ST-2) were employed in WST-8 assay to assess cell viability, cell morphology, and proliferation. The results showed that the presence of bioactive glass particles in the fibers enhances cell adhesion and proliferation compared to neat PCL mats. Furthermore, PCL/bioactive glass electrospun mats showed higher wound-healing rate (measured as cell migration rate) in vitro compared to neat PCL electrospun mats. Therefore, the characteristics of the PCL matrix combined with biological properties of bioactive glasses make PCL/bioactive glass composite ideal candidate for biomedical application.

scholarly journals Chitosan-Based Bioactive Glass Gauze: Microstructural Properties, In Vitro Bioactivity, and Biological Tests

Materials ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 2819 ◽  
Author(s):  
Rachele Sergi ◽  
Devis Bellucci ◽  
Roberta Salvatori ◽  
Valeria Cannillo
Keyword(s):  
Wound Healing ◽  
Cell Adhesion ◽  
Bioactive Glass ◽  
Organic Matrix ◽  
Scratch Test ◽  
Neutral Red ◽  
Wound Dressings ◽  
Nih 3T3 ◽  
Cell Adhesion And Proliferation

Passive commercial gauzes were turned into interactive wound dressings by impregnating them with a chitosan suspension. To further improve healing, and cell adhesion and proliferation, chitosan/bioactive glass wound dressings were produced with the addition of (i) 45S5, (ii) a Sr- and Mg-containing bioactive glass, and (iii) a Zn-containing bioactive glass to the chitosan suspension. SEM and FTIR analyses evidenced positive results in terms of incorporation of bioactive glass particles. Bioactivity was investigated by soaking chitosan-based bioactive glass wound dressings in simulated body fluid (SBF). Cell viability, proliferation, and morphology were investigated using NIH 3T3 (mouse embryonic fibroblast) cells by neutral red (NR) uptake and MTT assays. Furthermore, the wound-healing rate was evaluated by means of the scratch test, using NIH 3T3. The results showed that bioactive glass particles enhance cell adhesion and proliferation, and wound healing compared to pure chitosan. Therefore, chitosan-based bioactive glass wound dressings combine the properties of the organic matrix with the specific biological characteristics of bioactive glasses to achieve chitosan composites suitable for healing devices.

scholarly journals Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization

2018 ◽  
Vol 9 (4) ◽  
pp. 62 ◽  
Author(s):  
Gianluca Turco ◽  
Davide Porrelli ◽  
Eleonora Marsich ◽  
Federica Vecchies ◽  
Teresa Lombardi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Clinical Performance ◽  
Maxillofacial Surgery ◽  
Chemical Characterization ◽  
Bone Substitutes ◽  
Human Cell Line ◽  
Structural Features ◽  
Oral And Maxillofacial Surgery ◽  
Cell Adhesion And Proliferation

Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials.

scholarly journals In Vitro Assessment of the Functional Dynamics of Titanium with Surface Coating of Hydroxyapatite Nanoparticles

Materials ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 840 ◽  
Author(s):  
José Henrique de Lima Cavalcanti ◽  
Patrícia Matos ◽  
Cresus Vinícius Depes de Gouvêa ◽  
Waldimir Carvalho ◽  
José Luis Calvo-Guirado ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Formation ◽  
Titanium Implants ◽  
Tissue Response ◽  
Mesenchymal Progenitor Cells ◽  
Machined Surface ◽  
Implant Surface ◽  
Sem Images ◽  
Vitro Assay

Manipulation of implant surface characteristics constitutes a promising strategy for improving cell growth and tissue response on a variety of materials with different surface topographies. Mesenchymal progenitor cells with a capacity to respond to titanium surface stimuli and differentiate into osteoblasts were used to perform comparative tests between two different implant topographies, including their functional interaction with pre-osteoblasts directly seeded onto the implants. Functional analysis of nanostructured implant surfaces was performed by in vitro assay analysis. The machined surface of titanium implants (mach group) was used as a control and compared with a nanoparticle HA activated surface implant (nano group), developed by the deposition of pure crystalline hydroxyapatite. Cell culture on the nano group surface resulted in higher cell adhesion and cultured osteoblast viability compared with the mach group. Scanning electron microscope (SEM) images revealed a stable interaction, indicated by the presence of focal cell adhesion formation. These results together with positive mineralization assays showed the nano group to be an excellent scaffold for bone-implant integration.

Murine Macrophage RAW264.7 Cells Response for the Carbon Nanotubes Immobilized on Substrate

2012 ◽  
Vol 529-530 ◽  
pp. 379-384
Author(s):  
Tsukasa Akasaka ◽  
Shigeaki Abe ◽  
Fumio Watari
Keyword(s):  
Carbon Nanotubes ◽  
Cell Adhesion ◽  
Raw264.7 Cells ◽  
Murine Macrophage ◽  
Proliferation Assay ◽  
Tnf Α ◽  
Raw264.7 Cell ◽  
Cytotoxic Studies ◽  
Cell Adhesion And Proliferation

The reports on cytotoxic studies of carbon nanotubes (CNTs) increased exponentially. In the present study, we investigate murine macrophage RAW264.7 cell response for the CNTs immobilized on a polystyrene substrate. We prepared CNT-coated dishes, and estimate the interaction of RAW264.7 cells with CNTs by cell adhesion, proliferation assay, and measurement of TNF-α production. As a result, the highest cell adhesion and proliferation was observed on a commercially cell culture polystyrene dish, while CNT-coated dish indicate slightly lower activity of them. Moreover, amount of production of TNF-α on the CNT-coated dishes was considerable lower than that in the case of lipopolysaccharide (LPS) addition as a control. These results indicated that CNT-coated dishes could not show strong cytotoxicity for RAW264.7 cellsin vitro.

scholarly journals SIBS triblock copolymers in cardiac surgery: in vitro and in vivo studies in comparison with ePTFE

2020 ◽  
Vol 21 (4) ◽  
pp. 67-80
Author(s):  
M. A. Rezvova ◽  
E. A. Ovcharenko ◽  
P. A. Nikishev ◽  
S. V. Kostyuk ◽  
L. V. Antonova ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Heart Valves ◽  
Ex Vivo ◽  
Calcium Content ◽  
Positive Control ◽  
Prosthetic Heart Valves ◽  
Solution Casting Method ◽  
Cell Adhesion And Proliferation

Implantation of polymeric heart valves can solve the problems of existing valve substitutes – mechanical and biological. Objective: to comprehensively assess the hemocompatibility of styrene-isobutylene-styrene (SIBS) triblock copolymer, synthesized by controlled cationic polymerization in comparison with expanded polytetrafluoroethylene (ePTFE) used in clinical practice. Materials and methods. SIBS-based films were made by polymer solution casting method; in vitro biocompatibility assessment was performed using cell cultures, determining cell viability, cell adhesion and proliferation; tendency of materials to calcify was determined through in vitro accelerated calcification; in vivo biocompatibility assessment was performed by subcutaneous implantation of rat samples; hemocompatibility was determined ex vivo by assessing the degree of hemolysis, aggregation, and platelet adhesion. Results. The molecular weight of synthesized polymer was 33,000 g/mol with a polydispersity index of 1.3. When studying cell adhesion, no significant differences (p = 0.20) between the properties of the SIBS polymer (588 cells/mm2) and the properties of culture plastics (732 cells/mm2) were discovered. Cell adhesion for the ePTFE material was 212 cells/mm2. Percentage of dead cells on SIBS and ePTFE samples was 4.40 and 4.72% (p = 0.93), respectively, for culture plastic – 1.16% (p < 0.05). Cell proliferation on the ePTFE surface (0.10%) was significantly lower (p < 0.05) than for the same parameters for SIBS and culture plastic (62.04 and 44.00%). Implantation results (60 days) showed the formation of fibrous capsules with average thicknesses of 42 μm (ePTFE) and 58 μm (SIBS). Calcium content in the explanted samples was 0.39 mg/g (SIBS), 1.25 mg/g (ePTFE) and 93.79 mg/g (GA-xenopericardium) (p < 0.05). Hemolysis level of red blood cells after contact with SIBS was 0.35%, ePTFE – 0.40%, which is below positive control (p < 0.05). Maximum platelet aggregation of intact platelet-rich blood plasma was 8.60%, in contact with SIBS polymer – 18.11%, with ePTFE – 22.74%. Conclusion. In terms of hemocompatibility properties, the investigated SIBS polymer is not inferior to ePTFE and can be used as a basis for development of polymeric prosthetic heart valves.

scholarly journals Effect of honeycomb film on protein adsorption, cell adhesion and proliferation

2006 ◽  
Vol 284-285 ◽  
pp. 548-551 ◽  
Author(s):  
Hiroshi Sunami ◽  
Emiko Ito ◽  
Masaru Tanaka ◽  
Sadaaki Yamamoto ◽  
Masatsugu Shimomura
Keyword(s):  
Cell Adhesion ◽  
Protein Adsorption ◽  
Cell Adhesion And Proliferation

In-vitro cell adhesion and proliferation of adipose derived stem cell on hydroxyapatite composite surfaces

2017 ◽  
Vol 75 ◽  
pp. 1305-1316 ◽  
Author(s):  
Praneetha Pulyala ◽  
Akshay Singh ◽  
Marcela Ferreira Dias-Netipanyj ◽  
Sheron Compos Cogo ◽  
Luciane S. Santos ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Hydroxyapatite Composite ◽  
Adipose Derived Stem Cell ◽  
Cell Adhesion And Proliferation
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