scholarly journals PEG-b-PLGA Nanoparticles Loaded with Geraniin from Phyllanthus Watsonii Extract as a Phytochemical Delivery Model

2020 ◽  
Vol 10 (14) ◽  
pp. 4891
Author(s):  
Kogilavanee Devasvaran ◽  
Sreegayathri Jairaman ◽  
Nur Azirah Yahaya ◽  
Indu Bala S. Jaganath ◽  
Yit Lung Khung ◽  
...  
Keyword(s):  
High Performance ◽  
Double Emulsion ◽  
Plga Nanoparticles ◽  
Burst Release ◽  
Colon Cells ◽  
Emulsion Method ◽  
Simple Model System ◽  
Normal Human ◽  
Poly Ethylene ◽  
Plga Nanoparticle

The study outlined a standardized double emulsion method for simple poly(ethylene glycol)-block-poly(lactic-co-glycolic acid) (PEG-b-PLGA) nanoparticle (NP) synthesis. The PEG-b-PLGA NP was also used for entrapment of geraniin as a simple model system for phytochemical delivery. PEG-b-PLGA NPs were prepared using the double emulsion method. The yields and particle sizes of PEG-b-PLGA NPs obtained with and without encapsulation of geraniin were 57.6% and 134.20 ± 1.45 nm and 66.7% and 102.70 ± 12.36 nm, respectively. High-performance liquid chromatography of geraniin that was extracted from Phyllanthus watsonii was detected at 64 min. Geraniin burst release began at 40 min and fully released at 3 h. PEG-b-PLGA NP was non-cytotoxic, while cytotoxicity of geraniin was dose dependant towards normal human epithelial colon cells, CCD 841 CoN cells.

scholarly journals Quality by design prospects of pharmaceuticals application of double emulsion method for PLGA loaded nanoparticles

2021 ◽  
Vol 3 (6) ◽  
Author(s):  
Dhananjay Panigrahi ◽  
Pratap Kumar Sahu ◽  
Suryakanta Swain ◽  
Rajan Kumar Verma
Keyword(s):  
Drug Delivery ◽  
Quality By Design ◽  
Drug Product ◽  
Double Emulsion ◽  
Plga Nanoparticles ◽  
Influential Factors ◽  
Critical Material ◽  
Emulsion Method ◽  
Hydrophilic Drugs ◽  
Wide Range

Abstract QbD approach empowers the pharma researchers to minimize the number of experimental trials and time. It helps identify the significant, influential factors such as critical material attributes, critical formulation variables, and critical process parameters, which may significantly impact the quality of the products. Poly lactic-co-glycolic acid (PLGA), a biocompatible and biodegradable polymer, has gained an immense potential and wide range of applications as a carrier for manufacturing of polymeric nanoparticle drug delivery systems as per US-FDA and European Medicine Agency for drug delivery. The double emulsion method for preparing PLGA nanoparticles to encapsulate hydrophilic drugs has attracted interest in manufacturing processes. The double emulsion is a two-step process consisting of two different emulsification, making the process more complicated. The stability of nanoparticles obtained by a double emulsion method remains questionable due to the many formulations and process attributes. Currently, PLGA based nanoparticles prepared by a double emulsion technique are an alternative pharmaceutical manufacturing operation for getting the quality product by employing the Quality by Design approach. This present review has discussed the QbD elements to elucidate the effect of material attributes, formulation, and process variables on the critical quality attributes of the drug product, such as particle size distribution, encapsulation efficiency, etc. The components of a double emulsion, characteristics of drugs, polymers, and stabilizers used have been discussed in detail in this review. Graphic abstract

scholarly journals Ligand Decorated Embelin Loaded PLGA Nanoparticles for Management of Alcohol Induced Hepatotoxicity

2020 ◽  
Vol 10 (1) ◽  
pp. 72-80
Author(s):  
Ajay Kumar ◽  
M. Nikhat Khan ◽  
Jovita Kanoujia ◽  
Amandeep Singh ◽  
Neeraj Mishra
Keyword(s):  
High Performance ◽  
In Vitro Release ◽  
Plga Nanoparticles ◽  
Hep G2 ◽  
Release Studies ◽  
Plga Nanoparticle ◽  
Surface Modified ◽  
Vitro Release

Preparation of surface modified Embelin loaded nanoparticles (GA-PEG-PLGA) for the management of hepatotoxicity. Surface modified Embelin loaded GA-PEG-PLGA NPs were evaluated by NMR, FTIR, TEM techniques and in vitro release studies. The biodistribution of the nanoparticles was assessed by High-performance liquid chromatography (HPLC), and the cellular uptake study was evaluated using Hep G2 cells (liver cells lines). The hepatoprotecttive effect of the surface modified Embelin loaded GA-PEG-PLGA NPs was investigated in-vitro and in-vivo. The surface modified Embelin loaded GA-PEG-PLGA nanoparticles significantly increases the uptake of drug in liver by 2.5 folds more than plain drug. Keywords: Glycyrrhetinic acid, Receptor mediated, Surface functionalization, Embelin, PLGA nanoparticle.

The influence of extracts from Potentilla species on normal human colon cells

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
R Paduch ◽  
M Tomczyk ◽  
A Wiater ◽  
M Pleszczynska ◽  
M Kandefer Szerszen ◽  
...  
Keyword(s):  
Human Colon ◽  
Colon Cells ◽  
Normal Human

scholarly journals Native Chilean Berries Preservation and In Vitro Studies of a Polyphenol Highly Antioxidant Extract from Maqui as a Potential Agent against Inflammatory Diseases

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 843
Author(s):  
Tamara Ortiz ◽  
Federico Argüelles-Arias ◽  
Belén Begines ◽  
Josefa-María García-Montes ◽  
Alejandra Pereira ◽  
...  
Keyword(s):  
High Performance ◽  
Large Scale ◽  
Inflammatory Diseases ◽  
In Vitro Studies ◽  
Aminosalicylic Acid ◽  
Colon Cells ◽  
Antioxidant Power ◽  
Maqui Berry ◽  
Physiological Benefits

The best conservation method for native Chilean berries has been investigated in combination with an implemented large-scale extract of maqui berry, rich in total polyphenols and anthocyanin to be tested in intestinal epithelial and immune cells. The methanolic extract was obtained from lyophilized and analyzed maqui berries using Folin–Ciocalteu to quantify the total polyphenol content, as well as 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) to measure the antioxidant capacity. Determination of maqui’s anthocyanins profile was performed by ultra-high-performance liquid chromatography (UHPLC-MS/MS). Viability, cytotoxicity, and percent oxidation in epithelial colon cells (HT-29) and macrophages cells (RAW 264.7) were evaluated. In conclusion, preservation studies confirmed that the maqui properties and composition in fresh or frozen conditions are preserved and a more efficient and convenient extraction methodology was achieved. In vitro studies of epithelial cells have shown that this extract has a powerful antioxidant strength exhibiting a dose-dependent behavior. When lipopolysaccharide (LPS)-macrophages were activated, noncytotoxic effects were observed, and a relationship between oxidative stress and inflammation response was demonstrated. The maqui extract along with 5-aminosalicylic acid (5-ASA) have a synergistic effect. All of the compiled data pointed out to the use of this extract as a potential nutraceutical agent with physiological benefits for the treatment of inflammatory bowel disease (IBD).

Target-Specific Cellular Uptake of PLGA Nanoparticles Coated with Poly(l-lysine)−Poly(ethylene glycol)−Folate Conjugate

Langmuir ◽  
2005 ◽  
Vol 21 (19) ◽  
pp. 8852-8857 ◽  
Author(s):  
Sun Hwa Kim ◽  
Ji Hoon Jeong ◽  
Ki Woo Chun ◽  
Tae Gwan Park
Keyword(s):  
Ethylene Glycol ◽  
Cellular Uptake ◽  
Plga Nanoparticles ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Folate Conjugate

Facile synthesis of photoluminescent inorganic–organic hybrid carbon dots codoped with B and N: towards an efficient lubrication additive

2017 ◽  
Vol 46 (36) ◽  
pp. 12306-12312 ◽  
Author(s):  
Yunxiao Zhang ◽  
Tao Cai ◽  
Wangji Shang ◽  
Dan Liu ◽  
Qiang Guo ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Carbon Dots ◽  
High Performance ◽  
Poly Ethylene Glycol ◽  
Facile Synthesis ◽  
Organic Hybrid ◽  
Wear And Friction ◽  
Lubrication Additive ◽  
Poly Ethylene ◽  
Hybrid Carbon

An inorganic-organic carbon dots consisting of an ionic liquid moiety inserted in the carbon skeleton has been synthesized and verified to be a high-performance anti-wear and friction-reducing lubricant additives in poly(ethylene glycol)(PEG).

scholarly journals High-Performance Resistive Pressure Sensor Based on Elastic Composite Hydrogel of Silver Nanowires and Poly(ethylene glycol)

Micromachines ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 438 ◽  
Author(s):  
Youngsang Ko ◽  
Dabum Kim ◽  
Goomin Kwon ◽  
Jungmok You
Keyword(s):  
Composite Material ◽  
Ethylene Glycol ◽  
Pressure Sensor ◽  
High Performance ◽  
Silver Nanowires ◽  
High Sensitivity ◽  
Composite Hydrogel ◽  
Poly Ethylene Glycol ◽  
Pressure Sensing ◽  
Poly Ethylene

Improved pressure sensing is of great interest to enable the next-generation of bioelectronics systems. This paper describes the development of a transparent, flexible, highly sensitive pressure sensor, having a composite sandwich structure of elastic silver nanowires (AgNWs) and poly(ethylene glycol) (PEG). A simple PEG photolithography was employed to construct elastic AgNW-PEG composite patterns on flexible polyethylene terephthalate (PET) film. A porous PEG hydrogel structure enabled the use of conductive AgNW patterns while maintaining the elasticity of the composite material, features that are both essential for high-performance pressure sensing. The transparency and electrical properties of AgNW-PEG composite could be precisely controlled by varying the AgNW concentration. An elastic AgNW-PEG composite hydrogel with 0.6 wt % AgNW concentration exhibited high transmittance including T550nm of around 86%, low sheet resistance of 22.69 Ω·sq−1, and excellent bending durability (only 5.8% resistance increase under bending to 10 mm radius). A flexible resistive pressure sensor based on our highly transparent AgNW-PEG composite showed stable and reproducible response, high sensitivity (69.7 kPa−1), low sensing threshold (~2 kPa), and fast response time (20–40 ms), demonstrating the effectiveness of the AgNW-PEG composite material as an elastic conductor.

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