scholarly journals Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System

Antioxidants ◽  
2017 ◽  
Vol 6 (2) ◽  
pp. 35 ◽  
Author(s):  
Kamal Panda ◽  
Dambaru Golari ◽  
A. Venugopal ◽  
V. Achary ◽  
Ganngam Phaomei ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Lathyrus Sativus ◽  
Bioassay System ◽  
Root Bioassay ◽  
Lathyrus Sativus L

scholarly journals The Radiosensitizing Effect of Zinc Oxide Nanoparticles in Sub-Cytotoxic Dosing Is Associated with Oxidative Stress In Vitro

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4062
Author(s):  
Till Jasper Meyer ◽  
Agmal Scherzad ◽  
Helena Moratin ◽  
Thomas Eckert Gehrke ◽  
Julian Killisperger ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Oxidative Dna Damage ◽  
Clonogenic Survival ◽  
Oxide Nanoparticles ◽  
Radiosensitizing Effect ◽  
Primary Fibroblasts

Radioresistance is an important cause of head and neck cancer therapy failure. Zinc oxide nanoparticles (ZnO-NP) mediate tumor-selective toxic effects. The aim of this study was to evaluate the potential for radiosensitization of ZnO-NP. The dose-dependent cytotoxicity of ZnO-NP20 nm and ZnO-NP100 nm was investigated in FaDu and primary fibroblasts (FB) by an MTT assay. The clonogenic survival assay was used to evaluate the effects of ZnO-NP alone and in combination with irradiation on FB and FaDu. A formamidopyrimidine-DNA glycosylase (FPG)-modified single-cell microgel electrophoresis (comet) assay was applied to detect oxidative DNA damage in FB as a function of ZnO-NP and irradiation exposure. A significantly increased cytotoxicity after FaDu exposure to ZnO-NP20 nm or ZnO-NP100 nm was observed in a concentration of 10 µg/mL or 1 µg/mL respectively in 30 µg/mL of ZnO-NP20 nm or 20 µg/mL of ZnO-NP100 nm in FB. The addition of 1, 5, or 10 µg/mL ZnO-NP20 nm or ZnO-NP100 nm significantly reduced the clonogenic survival of FaDu after irradiation. The sub-cytotoxic dosage of ZnO-NP100 nm increased the oxidative DNA damage compared to the irradiated control. This effect was not significant for ZnO-NP20 nm. ZnO-NP showed radiosensitizing properties in the sub-cytotoxic dosage. At least for the ZnO-NP100 nm, an increased level of oxidative stress is a possible mechanism of the radiosensitizing effect.

scholarly journals Antibacterial Activity and Mechanism of Action of Zinc Oxide Nanoparticles againstCampylobacter jejuni

2011 ◽  
Vol 77 (7) ◽  
pp. 2325-2331 ◽  
Author(s):  
Yanping Xie ◽  
Yiping He ◽  
Peter L. Irwin ◽  
Tony Jin ◽  
Xianming Shi
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Stress Response ◽  
Quantitative Pcr ◽  
Zno Nanoparticles ◽  
Morphological Changes ◽  
Toxin Production ◽  
Response To Treatment ◽  
Large Set ◽  
Zno Nanoparticle

ABSTRACTThe antibacterial effect of zinc oxide (ZnO) nanoparticles onCampylobacter jejuniwas investigated for inhibition and inactivation of cell growth. The results showed thatC. jejuniwas extremely sensitive to treatment with ZnO nanoparticles. The MIC of ZnO nanoparticles forC. jejuniwas determined to be 0.05 to 0.025 mg/ml, which is 8- to 16-fold lower than that forSalmonella entericaserovar Enteritidis andEscherichia coliO157:H7 (0.4 mg/ml). The action of ZnO nanoparticles againstC. jejuniwas determined to be bactericidal, not bacteriostatic. Scanning electron microscopy examination revealed that the majority of the cells transformed from spiral shapes into coccoid forms after exposure to 0.5 mg/ml of ZnO nanoparticles for 16 h, which is consistent with the morphological changes ofC. jejuniunder other stress conditions. These coccoid cells were found by ethidium monoazide-quantitative PCR (EMA-qPCR) to have a certain level of membrane leakage. To address the molecular basis of ZnO nanoparticle action, a large set of genes involved in cell stress response, motility, pathogenesis, and toxin production were selected for a gene expression study. Reverse transcription-quantitative PCR (RT-qPCR) showed that in response to treatment with ZnO nanoparticles, the expression levels of two oxidative stress genes (katAandahpC) and a general stress response gene (dnaK) were increased 52-, 7-, and 17-fold, respectively. These results suggest that the antibacterial mechanism of ZnO nanoparticles is most likely due to disruption of the cell membrane and oxidative stress inCampylobacter.

scholarly journals Ameliorative effect of biofabricated ZnO nanoparticles ofTrianthema portulacastrumLinn. on dermal woundsviaremoval of oxidative stress and inflammation

RSC Advances ◽  
2018 ◽  
Vol 8 (38) ◽  
pp. 21621-21635 ◽  
Author(s):  
Ekta Yadav ◽  
Deepika Singh ◽  
Pankajkumar Yadav ◽  
Amita Verma
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Ameliorative Effect

The curative efficacy of a green synthesized zinc oxide nano-ointment on wound healing was investigated.

Genotoxic and oxidative stress potential of nanosized and bulk zinc oxide particles in Drosophila melanogaster

2016 ◽  
Vol 32 (12) ◽  
pp. 1987-2001 ◽  
Author(s):  
Erico R Carmona ◽  
Claudio Inostroza-Blancheteau ◽  
Laura Rubio ◽  
Ricard Marcos
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Dna Damage ◽  
Zinc Oxide ◽  
Drosophila Melanogaster ◽  
Comet Assay ◽  
Food Additives ◽  
Spot Test ◽  
Genotoxic Activity ◽  
And Oxidative Stress

Zinc oxide nanoparticles (ZnONP) are manufactured on a large scale and can be found in a variety of consumer products, such as sunscreens, lotions, paints and food additives. Few studies have been carried out on its genotoxic potential and related mechanisms in whole organisms. In the present study, the in vivo genotoxic activity of ZnONP and its bulk form was assayed using the wing-spot test and comet assay in Drosophila melanogaster. Additionally, a lipid peroxidation analysis using the thiobarbituric acid assay was also performed. Results obtained with the wing-spot test showed a lack of genotoxic activity of both ZnO forms. However, when both particle sizes were tested in the comet assay using larvae haemocytes, a significant increase in DNA damage was observed for ZnONP treatments but only at the higher dose applied. In addition, the lipid peroxidation assay showed significant malondialdehyde (MDA) induction for both ZnO forms, but the induction of MDA for ZnONP was higher for the ZnO bulk, suggesting that the observed DNA strand breaks could be induced by mediated oxidative stress. The overall data suggest that the potential genotoxicity of ZnONP in Drosophila can be considered weak according to the lack of mutagenic and recombinogenic effects and the induction of primary DNA damage only at high toxic doses of ZnONP. This study is the first assessing the genotoxic and oxidative stress potential of nano and bulk ZnO particles in Drosophila.

Toxicity of zinc oxide (ZnO) nanoparticles on human bronchial epithelial cells (BEAS-2B) is accentuated by oxidative stress

2010 ◽  
Vol 48 (6) ◽  
pp. 1762-1766 ◽  
Author(s):  
Boon Chin Heng ◽  
Xinxin Zhao ◽  
Sijing Xiong ◽  
Kee Woei Ng ◽  
Freddy Yin-Chiang Boey ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Epithelial Cells ◽  
Zno Nanoparticles ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

Ameliorative Role of Green Tea and Zinc Oxide Nanoparticles Complex Against Monosodium Glutamate-Induced Testicular Toxicity in Male Rats

2020 ◽  
Vol 21 (6) ◽  
pp. 488-501 ◽  
Author(s):  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Hebatullah Laban ◽  
Nahla S. El-Shenawy
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Male Rats ◽  
Histological Structure ◽  
Oxide Nanoparticles ◽  
Testosterone Levels

Background and Objective: This study was designed to estimate the long-term effects of zinc oxide nanoparticles/green tea (ZnONPs/GTE) complex against monosodium glutamate (MSG). The antioxidant/oxidative status, testosterone levels, DNA damage, and histopathological changes of testis were evaluated. Methods: The rats were divided into eight groups that were treated as follows: saline, the lower dosage of MSG (6.0 mg/kg), the higher dosage of MSG (17.5 mg/Kg), GTE, ZnONPs, ZnONPs/GTE and the last two groups were treated with the lower dosage of MSG or the higher dosage of MSG with ZnONPs/GTE complex. The data showed minimal toxicity in testicular tissue after the administration of ZnONPs. Results: The MSG treatment in the adult male rats reduced testosterone levels and disrupted testicular histology, which revealed dose-dependence of MSG. Also, ZnONPs induced testicular dysfunction through the interference of antioxidant/oxidant balance and suppression of testosterone levels as well as induction of cellular damage of testis. The combination of ZnONPs with GTE complex significantly protects against MSG or ZnONPs toxicity by decreasing the DNA damage, oxidative stress, and enhancement of antioxidant as well as histological structure of testis. Conclusion: We could recommend using ZnONPs/GTE complex to reduce the toxicity of ZnONPs and MSG on the testis at the cellular and oxidative stress levels.

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