scholarly journals Induction of oxidative stress, DNA damage, and apoptosis in a malignant human skin melanoma cell line after exposure to zinc oxide nanoparticles

2013 ◽  
pp. 983 ◽  
Author(s):  
Daoud Ali ◽  
Alarifi ◽  
Saad Alkahtani ◽  
Ankit Verma ◽  
Ahamed ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Zinc Oxide ◽  
Cell Line ◽  
Human Skin ◽  
Melanoma Cell ◽  
Zinc Oxide Nanoparticles ◽  
Melanoma Cell Line ◽  
Oxide Nanoparticles ◽  
Skin Melanoma

scholarly journals The Radiosensitizing Effect of Zinc Oxide Nanoparticles in Sub-Cytotoxic Dosing Is Associated with Oxidative Stress In Vitro

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4062
Author(s):  
Till Jasper Meyer ◽  
Agmal Scherzad ◽  
Helena Moratin ◽  
Thomas Eckert Gehrke ◽  
Julian Killisperger ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Oxidative Dna Damage ◽  
Clonogenic Survival ◽  
Oxide Nanoparticles ◽  
Radiosensitizing Effect ◽  
Primary Fibroblasts

Radioresistance is an important cause of head and neck cancer therapy failure. Zinc oxide nanoparticles (ZnO-NP) mediate tumor-selective toxic effects. The aim of this study was to evaluate the potential for radiosensitization of ZnO-NP. The dose-dependent cytotoxicity of ZnO-NP20 nm and ZnO-NP100 nm was investigated in FaDu and primary fibroblasts (FB) by an MTT assay. The clonogenic survival assay was used to evaluate the effects of ZnO-NP alone and in combination with irradiation on FB and FaDu. A formamidopyrimidine-DNA glycosylase (FPG)-modified single-cell microgel electrophoresis (comet) assay was applied to detect oxidative DNA damage in FB as a function of ZnO-NP and irradiation exposure. A significantly increased cytotoxicity after FaDu exposure to ZnO-NP20 nm or ZnO-NP100 nm was observed in a concentration of 10 µg/mL or 1 µg/mL respectively in 30 µg/mL of ZnO-NP20 nm or 20 µg/mL of ZnO-NP100 nm in FB. The addition of 1, 5, or 10 µg/mL ZnO-NP20 nm or ZnO-NP100 nm significantly reduced the clonogenic survival of FaDu after irradiation. The sub-cytotoxic dosage of ZnO-NP100 nm increased the oxidative DNA damage compared to the irradiated control. This effect was not significant for ZnO-NP20 nm. ZnO-NP showed radiosensitizing properties in the sub-cytotoxic dosage. At least for the ZnO-NP100 nm, an increased level of oxidative stress is a possible mechanism of the radiosensitizing effect.

Synthesis of N-aryl-2,6-diphenyl-2H-pyrazolo[4,3-c]pyridin-7-amines and their photodynamic properties in the human skin melanoma cell line G361

2021 ◽  
pp. 105570
Author(s):  
Beatričė Razmienė ◽  
Veronika Vojáčková ◽  
Eva Řezníčková ◽  
Lukáš Malina ◽  
Vaida Dambrauskienė ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Skin ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Skin Melanoma

Synthesis and photodynamic properties of pyrazole-indole hybrids in the human skin melanoma cell line G361

2020 ◽  
Vol 183 ◽  
pp. 108666
Author(s):  
Gabrielė Varvuolytė ◽  
Lukáš Malina ◽  
Aurimas Bieliauskas ◽  
Barbora Hošíková ◽  
Helena Simerská ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Skin ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Skin Melanoma

scholarly journals Isolation and in vitro cytotoxic activity of 11-methylixoside isolated from bark of Randia dumetorum Lamk

2013 ◽  
Vol 59 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Jeet Singh Jangwan ◽  
Rita Patrizia Aquino ◽  
Teresa Mencherini ◽  
Raghubir Singh
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Human Skin ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Neuroblastoma Cell ◽  
Skin Melanoma ◽  
Iridoid Glucoside ◽  
Medicinal Tree

Summary Randia dumetorum (family Rubiaceae) is highly reputed ayurvedic medicinal tree commonly known as the Mainphal. A large deciduous thorny shrub grows up to 5 m of height. It occurs almost throughout India up to 1200 m of altitude. It is found in Himalaya from Jammu East ward ascending to 400 m and from Kashmir to East ward up to 1200 m. 11-methylixoside (compound 1), an iridoid glucoside, was isolated from the bark of this plant. The structure was characterized by using spectroscopic methods including 1D-1HNMR,13C-NMR and 2D-NMR (HSQC,HMBC, DQF-COSY) experiments and confirmed by comparison of their NMR data with those from the literature. This compound has been reported for the first time in Randia dumetorum bark. The 11-methylixoside was subjected to cytotoxic activity against MDA-MB-231 (breast cancer cell line) and SK-MEL-2 (human skin melanoma cell line), BE(2)C (neuroblastoma cell line derived from human bone marrow) and U87MG (human neuronale glioblastoma (astrozytom) cell line showed appreciable cytotoxic effect with IC50 value 63.10 µg/ml concentration for SK-MEL-2 (human skin melanoma cell line).

Ameliorative Role of Green Tea and Zinc Oxide Nanoparticles Complex Against Monosodium Glutamate-Induced Testicular Toxicity in Male Rats

2020 ◽  
Vol 21 (6) ◽  
pp. 488-501 ◽  
Author(s):  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Hebatullah Laban ◽  
Nahla S. El-Shenawy
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Male Rats ◽  
Histological Structure ◽  
Oxide Nanoparticles ◽  
Testosterone Levels

Background and Objective: This study was designed to estimate the long-term effects of zinc oxide nanoparticles/green tea (ZnONPs/GTE) complex against monosodium glutamate (MSG). The antioxidant/oxidative status, testosterone levels, DNA damage, and histopathological changes of testis were evaluated. Methods: The rats were divided into eight groups that were treated as follows: saline, the lower dosage of MSG (6.0 mg/kg), the higher dosage of MSG (17.5 mg/Kg), GTE, ZnONPs, ZnONPs/GTE and the last two groups were treated with the lower dosage of MSG or the higher dosage of MSG with ZnONPs/GTE complex. The data showed minimal toxicity in testicular tissue after the administration of ZnONPs. Results: The MSG treatment in the adult male rats reduced testosterone levels and disrupted testicular histology, which revealed dose-dependence of MSG. Also, ZnONPs induced testicular dysfunction through the interference of antioxidant/oxidant balance and suppression of testosterone levels as well as induction of cellular damage of testis. The combination of ZnONPs with GTE complex significantly protects against MSG or ZnONPs toxicity by decreasing the DNA damage, oxidative stress, and enhancement of antioxidant as well as histological structure of testis. Conclusion: We could recommend using ZnONPs/GTE complex to reduce the toxicity of ZnONPs and MSG on the testis at the cellular and oxidative stress levels.

Sunscreens containing zinc oxide nanoparticles can trigger oxidative stress and toxicity to the marine copepod Tigriopus japonicus

2020 ◽  
Vol 154 ◽  
pp. 111078 ◽  
Author(s):  
Stella W.Y. Wong ◽  
Guang-Jie Zhou ◽  
Priscilla T.Y. Leung ◽  
Jeonghoon Han ◽  
Jae-Seong Lee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Tigriopus Japonicus ◽  
Marine Copepod ◽  
Copepod Tigriopus Japonicus

Repetitive exposure to zinc oxide nanoparticles induces dna damage in human nasal mucosa mini organ cultures

2011 ◽  
Vol 52 (7) ◽  
pp. 582-589 ◽  
Author(s):  
Stephan Hackenberg ◽  
Franz-Zeno Zimmermann ◽  
Agmal Scherzed ◽  
Gudrun Friehs ◽  
Katrin Froelich ◽  
...  
Keyword(s):  
Dna Damage ◽  
Zinc Oxide ◽  
Nasal Mucosa ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Organ Cultures ◽  
Human Nasal Mucosa ◽  
Repetitive Exposure
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