scholarly journals Evaluation of Electroencephalography, Behaviour and Eye Temperature in Response to Surgical Castration in Sheep

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 637
Author(s):  
Charissa Harris ◽  
Peter John White ◽  
Evelyn Hall ◽  
Dominique Van der Saag ◽  
Sabrina Lomax
Keyword(s):  
Treatment Groups ◽  
Post Procedure ◽  
Eeg Changes ◽  
Testicular Injection ◽  
Ram Lambs ◽  
On Farm ◽  
Eye Temperature ◽  
Time Point ◽  
Ocular Temperature ◽  
Initial Change

Castration has been demonstrated to cause pain in sheep. However, it is routinely performed for management purposes. Electroencephalography (EEG) has been used successfully to measure pain in lambs in response to castration and other husbandry procedures in livestock. The aim of this study was to evaluate the use of EEG as a measure of pain and analgesia in conscious lambs undergoing castration on farm over a 24 h period. EEG responses were compared to behavioural observations and changes in ocular temperature via infrared thermography. Twenty-four merino ram lambs (18.63 ± 2.06 kg) were used in this study. Lambs were randomly allocated to one of the following treatment groups: (1) castration with pre-surgical administration of meloxicam (0.5 mg/kg Metacam 20 mg/mL injected subcutaneously into the skin of the neck 15 min prior to recording) and lignocaine (applied via intra-testicular injection five minutes prior to castration, 2 mL lignocaine hydrochloride 20 mg/mL, Troy/Ilium) (CML, n = 8); (2) castration only (C, n = 8); (3) sham castration, handling only (SC, n = 8). EEG was recorded for 5 mins pre-procedure (prior to any intervention), and for 5 mins post-procedure at 0, 1, 4 and 24 h. Behavioural reactions to the procedure were scored, and behaviours were scan sampled at 5 min intervals at the above time points, by blinded observers. Eye temperature was measured for five-minute intervals at each time point. EEG decreased from baseline to 0 h for CML and C groups (p < 0.001), C group values returned similar to baseline at 24 h. Eye temperature increased post-castration at 0 h for C group, no initial change was seen for CML or SC groups. CML and C groups were more likely to have higher reaction scores and showed more abnormal behaviours (p = 0.017). CML and C groups had similar results, indicating minimal effect of analgesic intervention. Lambs in SC group showed significant EEG changes, suggesting that stress from handling also impacted EEG results.

scholarly journals PSIII-4 Infrared thermography as a method for detecting pain in dairy calves in response to disbudding on-farm

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 190-191
Author(s):  
Mhairi Sutherland ◽  
Gemma Lowe ◽  
Neil Cox ◽  
Mairi Stewart
Keyword(s):  
Infrared Thermography ◽  
Mixed Model ◽  
Dairy Calves ◽  
Mixed Model Analysis ◽  
Treatment Groups ◽  
Before And After ◽  
Invasive Method ◽  
Feeding Bout ◽  
On Farm ◽  
Eye Temperature

Abstract Measured using infrared thermography (IRT), changes in eye temperature, can be used to non-invasively measure pain in response to husbandry procedures such as disbudding in calves. Previous studies have manually recorded IRT in controlled studies, however, it would be of interest to determine if IRT could be used to measure changes in eye temperature in response to pain in an automated on-farm system. Therefore, the objective of this study was to determine if IRT could be used to detect pain in dairy calves in response to disbudding on-farm. At 3 wk of age, 51 Friesian calves were allocated to 1 of 5 treatment groups: 1) sham handling (SHAM, n = 10), 2) cautery disbudding (DB, n = 11), 3) administration of local anesthetic (LA) and DB (LA+DB, n = 11), 4) administration of a non-steroidal anti-inflammatory drug (NSAID) and DB (NSAID+DB, n = 9) and 5) administration of LA, NSAID and DB (LA+NSAID+DB, n = 10). Eye temperature was measured using an IRT camera located next to an automatic calf milk feeder for 3 days before and after disbudding. During each calf’s visit to the feeder, IRT images were automatically recorded for the duration of the feeding bout. A mixed model analysis with splines was used to determine the effect of treatment on eye temperature over time. There was no overall effect of disbudding treatment on eye temperature (P = 0.22), but temperature appeared to increase in calves after disbudding or sham handling (P = 0.036). On average, calves visited the calf feeder 4 times/day, which may have been too infrequent to collect sufficient IRT images to detect a difference in eye temperature in response to disbudding. However, the increase in eye temperature after disbudding and handling may reflect a general stress response. Therefore, the automated use of IRT to measure eye temperature may be a useful non-invasive method to measure stress in calves.

scholarly journals Intrauterine lidocaine and naproxen for analgesia during intrauterine device insertion: randomized controlled trial

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Shana M. Miles ◽  
Katerina Shvartsman ◽  
Susan Dunlow
Keyword(s):  
Pain Assessment ◽  
Controlled Trial ◽  
Intrauterine Device ◽  
Self Medication ◽  
Double Blind ◽  
Clinical Trial Registration ◽  
Treatment Groups ◽  
Pain Scores ◽  
Post Procedure ◽  
Treatment Medication

Abstract Background This study evaluates oral naproxen and intrauterine instillation of lidocaine for analgesia with intrauterine device (IUD) placement as compared to placebo. Methods This was a randomized, double-blind, placebo-controlled trial. Patients desiring levonorgestrel 52 mg IUD or Copper T380A IUD were randomized into treatment groups. Patients received either oral naproxen 375 mg or placebo approximately 1 h prior to procedure in conjunction with 5 mL of 2% lidocaine or 5 mL of intrauterine saline. The primary outcome was pain with IUD insertion measured on a visual analog scale immediately following the procedure. Prespecified secondary outcomes included physician pain assessment, post procedure analgesia, satisfaction with procedure, satisfaction with IUD, and pain assessment related to IUD type. Results From June 4, 2014 to October 28, 2016 a total of 160 women desiring Copper T380A or levonorgestrel 52 mg intrauterine device insertion and meeting study criteria were enrolled and randomized in the study. Of these, 157 (78 in the Copper T380A arm, 79 in the levonorgestrel 52 mg) received study treatment medication. There were 39 in naproxen/lidocaine arm, 39 in placebo/lidocaine arm, 40 in naproxen/placebo arm, and 39 in placebo/placebo arm. There were no differences in the mean pain scores for IUD placement between treatment groups (naproxen/lidocaine 3.38 ± 2.49; lidocaine only 2.87 ± 2.13; naproxen only 3.09 ± 2.18; placebo 3.62 ± 2.45). There was no difference in self-medication post procedure or in satisfaction with the procedure and IUD among women in the treatment arms or by type of IUD. Conclusion Naproxen with or without intrauterine lidocaine does not reduce pain with IUD placement. Clinical trial registration Clinicaltrials.gov, NCT02769247. Registered May 11, 2016, Retrospectively registered

scholarly journals 83 Evaluation of transmammary-delivered firocoxib and an ethyl chloride spray to mitigate pain associated with processing procedures in piglets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 4-4
Author(s):  
Abbie V Viscardi ◽  
Elizabeth Shirtcliff ◽  
Emily Eppler ◽  
Savannah Miller ◽  
Johann Coetzee
Keyword(s):  
Production Systems ◽  
Ethyl Chloride ◽  
Hair Cortisol ◽  
Post Processing ◽  
Significant Treatment ◽  
Treatment Groups ◽  
Time Points ◽  
Tail Docking ◽  
Management Procedures ◽  
On Farm

Abstract Piglets raised in commercial production systems in the U.S. undergo painful management procedures, including surgical castration, tail docking and ear notching, without analgesia or anesthesia provision for pain relief. This is a significant animal welfare concern. There is an immediate need to identify the most practical and effective analgesia or anesthesia option for use on-farm. The objective of this study was to assess the efficacy of 2.0mg/kg firocoxib, administered to the sow and delivered transmammary to her piglets, and a vapocoolant spray (ethyl chloride) to reduce processing pain. Five-day old male and female Yorkshire-cross piglets were used. 2.0mg/kg firocoxib was administered to the sow intramuscularly 7h prior to processing piglets. An ethyl chloride spray was applied to the ears, tail and scrotum of the piglets immediately before ear notching, tail docking and surgical castration, respectively. Piglets were assigned to one of four treatment groups: firocoxib and vapocoolant spray (FV; n=32), firocoxib only (F; n=32), vapocoolant spray only (V; n=32), no treatment (CON; n=32). The observation period was from 24h pre- to 48h post-processing (specific time points = baseline, 0h, 1h, 2h, 4h, 7h, 24h, 30h, 36h, 48h). Preliminary results found piglets displayed significantly more pain-related behaviors at 24h and 30h post-processing than at most other time points (p&lt; 0.05). Piglets had significantly higher cranial temperatures at 7h post-processing than all other time points (p&lt; 0.05). There was a trend in FV and F piglets having a higher cranial temperature at 36h post-processing compared to V and CON piglets (p=0.08). All piglets had significantly higher hair cortisol levels at 4 vs 20-days old (p&lt; .0001); however, there were no significant treatment effects on cranial temperature, hair cortisol or pain behavior, suggesting firocoxib and the ethyl chloride spray were unable to significantly reduce piglet pain post-processing. Further study analysis is needed to confirm these initial findings.

scholarly journals AB0369 EFFICACY AND SAFETY OF RITUXIMAB ORIGINATOR AND BIOSIMILAR IN PRIMARY SJÖGREN’S SYNDROME IN A REAL-LIFE SETTING

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1485.3-1485
Author(s):  
F. Carubbi ◽  
A. Alunno ◽  
P. Cipriani ◽  
V. Pavlych ◽  
C. DI Muzio ◽  
...  
Keyword(s):  
Disease Activity ◽  
Real Life ◽  
Primary Sjögren’S Syndrome ◽  
Efficacy And Safety ◽  
Research Support ◽  
Treatment Groups ◽  
Real Life Setting ◽  
Patient Reported ◽  
Time Point

Background:Over the last 2 decades rituximab (RTX) has been widely used, albeit off-label, in primary Sjögren’s syndrome (pSS). Several studies reported that B-lymphocyte depletion with RTX is effective in this disease not only by reducing disease activity but also by affecting the inflammation and the lymphoid organization that occur in target tissues. With the recent release of several RTX biosimilars (bRTX) on the market, the demonstration of their interchangeability with RTX originator (oRTX) is required.Objectives:To compare efficacy and safety of oRTX and bRTX in pSS patients in a real-life setting.Methods:Clinical records of pSS patients referring to a tertiary rheumatology clinic were retrospectively evaluated. Patients having received at least 2 courses of either oRTX or bRTX (1000 mg IV infusion, repeated after 2 weeks -1 course- and the course repeated after 24 weeks) with complete data at baseline and after 3, 6, 9 and 12 months of treatment were enrolled. Disease activity was assessed with the EULAR SS disease activity index (ESSDAI) and its clinical version without the biological domain (ClinESSDAI). Patient-reported symptoms were assessed with the EULAR SS Patient Reported Index (ESSPRI).Results:Seven patients that received oRTX and 7 patients that received bRTX were enrolled. Baseline clinical features, including ESSDAI and ESSPRI were similar in the 2 treatment groups. Both compounds significantly reduced ESSDAI and ESSPRI as early as 3 months and no difference between the groups was observed at any time point (Figure 1). Of interest, ESSDAI slowly decreased until month 6 when the most pronounced reduction was observed. Conversely, ESSPRI dropped to its lowest values already at month 3. With regard to safety, at 12 months of follow-up no adverse event was observed in any of the treatment groups.Conclusion:At 12 months of follow-up, oRTX and bRTX display similar efficacy and safety profiles. The improvement of patient reported outcomes is faster than the improvement of disease activity with both compounds. Our data support interchangeability of oRTX and bRTX in pSS.References:[1]Carubbi F et al. Arthritis Res Ther. 2013;15(5):R172[2]Carubbi F et al. Lupus. 2014;23(13):1337-49Figure 1 ESSDAI and ESSPRI values at every time point in the 2 treatment groups. Asterisks indicate p values <0.05 compared to the other treatment group at the same time pointDisclosure of Interests:Francesco Carubbi Speakers bureau: Francesco Carubbi received speaker honoraria from Abbvie and Celgene outside this work., Alessia Alunno: None declared, Paola Cipriani Grant/research support from: Actelion, Pfizer, Speakers bureau: Actelion, Pfizer, Viktoriya Pavlych: None declared, claudia di muzio: None declared, Roberto Gerli: None declared, Roberto Giacomelli Grant/research support from: Actelion, Pfizer, Speakers bureau: Abbvie, Roche, Actelion, BMS, MSD, Ely Lilly, SOBI, Pfizer

Analysis of Histopathological and Ultrasonographic Testicular Changes Following Intra Testicular Administration of Zinc Gluconate and Chlorhexidine Gluconate in Adult Dogs

2021 ◽  
Author(s):  
Aeknath Virendra ◽  
Madhu Swamy ◽  
Nitin Bajaj ◽  
Satya Nidhi Shukla ◽  
Apra Shahi
Keyword(s):  
Testicular Biopsy ◽  
Group Iv ◽  
Chlorhexidine Gluconate ◽  
Basement Membranes ◽  
Zinc Gluconate ◽  
Treatment Groups ◽  
Contraceptive Effect ◽  
Stray Dog ◽  
Testicular Injection ◽  
Sexually Mature

Background: Escalating stray dog population is becoming a big problem worldwide. The chemical contraception is gaining more popularity because of its cost effectiveness, easy application and negligible complications. Methods: The present study was designed to analyze the contraceptive effect of intra-testicular injection of zinc gluconate neutralized with arginine and chlorhexidine gluconate in twenty-four sexually mature male mongrel dogs divided into four groups viz I, II, III and IV each of six animals. Groups I, II and III were treated with zinc gluconate neutralized with arginine, 5% and 4% chlorhexidine gluconate, respectively and group IV were kept as control. The testicular biopsy samples were collected on day 0 and 30 using 16-gauge biopsy gun. The ultrasonographic examination of testes was done on day 0, 5, 15, 24 and 30. Result: The noteworthy microscopic changes at day 30 such as irregular basement membranes with severe depletion of both germinal and sertoli cells were observed in all the treatment groups. The ultrasonography revealed degenerated testicles and significant diminution in the testicular length by day 30 in all the treatment groups. Thus, intra-testicular injection of zinc gluconate neutralized with arginine, 5% and 4% chlorhexidine gluconate may be used for chemical contraception in dogs.

scholarly journals An Analysis of Clostridium difficile Environmental Contamination During and After Treatment for C difficile Infection

2020 ◽  
Vol 7 (11) ◽  
Author(s):  
Kerrie Davies ◽  
Damian Mawer ◽  
A Sarah Walker ◽  
Claire Berry ◽  
Timothy Planche ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Environmental Contamination ◽  
Environmental Samples ◽  
Transmission Risk ◽  
Fecal Samples ◽  
Treatment Groups ◽  
End Of Treatment ◽  
Significant Difference ◽  
Time Point

Abstract Background Lower Clostridium difficile spore counts in feces from C difficile infection (CDI) patients treated with fidaxomicin versus vancomycin have been observed. We aimed to determine whether environmental contamination is lower in patients treated with fidaxomicin compared with those treated with vancomycin/metronidazole. Methods The CDI cases were recruited at 4 UK hospitals (Leeds, Bradford, and London [2 centers]). Environmental samples (5 room sites) were taken pretreatment and at 2–3, 4–5, 6–8, and 9–12 days of treatment, end of treatment (EOT), and post-EOT. Fecal samples were collected at diagnosis and as often as produced thereafter. Swabs/feces were cultured for C difficile; percentage of C difficile-positive samples and C difficile bioburden were compared between different treatment arms at each time point. Results Pre-EOT (n = 244), there was a significant reduction in environmental contamination (≥1 site positive) around fidaxomicin versus vancomycin/metronidazole recipients at days 4–5 (30% vs 50% recipients, P = .04) and at days 9–12 (22% vs 49%, P = .005). This trend was consistently seen at all other timepoints, but it was not statistically significant. No differences were seen between treatment groups post-EOT (n = 76). Fidaxomicin-associated fecal positivity rates and colony counts were consistently lower than those for vancomycin/metronidazole from days 4 to 5 of treatment (including post-EOT); however, the only significant difference was in positivity rate at days 9–12 (15% vs 55%, P = .03). Conclusions There were significant reductions in C difficile recovery from both feces and the environment around fidaxomicin versus vancomycin/metronidazole recipients. Therefore, fidaxomicin treatment may lower the C difficile transmission risk by reducing excretion and environmental contamination.

Peri-operative Adverse Outcomes in Patients with Atrial Fibrillation Taking Warfarin or Edoxaban: Analysis of the ENGAGE AF-TIMI 48 Trial

2018 ◽  
Vol 118 (06) ◽  
pp. 1001-1008 ◽  
Author(s):  
James Douketis ◽  
Sabina Murphy ◽  
Elliott Antman ◽  
Laura Grip ◽  
Michele Mercuri ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Invasive Procedure ◽  
Operative Management ◽  
Adverse Outcomes ◽  
Systemic Embolism ◽  
Chi Square ◽  
Treatment Groups ◽  
Post Procedure ◽  
Chi Square Test ◽  
Anticoagulated Patients

Background Peri-operative management of anticoagulated patients with atrial fibrillation (AF) is challenging. To gain information on the peri-operative management of edoxaban, we compared outcomes in patients on warfarin or edoxaban enrolled in ENGAGE AF-TIMI 48 who underwent a surgery or invasive procedure. Methods Data from patients undergoing their first surgery/procedure were analysed and results compared by anticoagulant (warfarin vs. higher- or lower-dose edoxaban regimen [HDER and LDER, respectively]). Patients were classified by procedural management: anticoagulant interrupted (last dose 4–10 days pre-procedure) or anticoagulant continued (last dose ≤ 3 days pre-procedure). Stroke/systemic embolism (SSE), major bleeding (MB), MB or clinically relevant non-MB (CRNMB) and death were assessed from 7 days pre- until 30 days post-procedure. The chi-square test was used to compare outcomes across treatment groups. Results A total of 7,193 patients (34%) underwent surgery/procedure: 3,116 had anticoagulant interrupted, 4,077 had anticoagulant continued. Among patients on warfarin, HDER and LDER who had anticoagulant interrupted, rates of SSE were 0.6, 0.5 and 0.9% (p = 0.53), rates of MB were 1.0, 1.2 and 1.1% (p = 0.94) and rates of MB or CRNMB were 3.9, 4.2 and 3.6% (p = 0.78); among patients on warfarin, HDER and LDER who had anticoagulant continued, rates of SSE were 1.1, 0.7 and 0.9% (p = 0.51), rates of MB were 3.6, 2.6 and 2.4% (p = 0.13) and rates of MB or CRNMB were 8.5, 7.9 and 6.6% (p = 0.17). Conclusion In patients requiring surgery/procedure in ENGAGE AF-TIMI 48, peri-operative rates of SSE, MB and death were not significantly different in patients who received edoxaban or warfarin.

scholarly journals Electroencephalography Can Distinguish between Pain and Anaesthetic Intervention in Conscious Lambs Undergoing Castration

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 428 ◽  
Author(s):  
Charissa Harris ◽  
Peter John White ◽  
Virginia L. Mohler ◽  
Sabrina Lomax
Keyword(s):  
Pain Relief ◽  
Local Anaesthesia ◽  
Pain Measurement ◽  
Conscious State ◽  
General Anaesthetic ◽  
Treatment Groups ◽  
Australian Sheep ◽  
Ram Lambs

Australian sheep routinely undergo painful surgical husbandry procedures without anaesthesia or analgesia. Electroencephalography (EEG) has been shown to be a successful measure of pain in livestock under a general anaesthetic. The aim of this study was to compare this EEG model to that of conscious lambs undergoing castration with and without local anaesthesia. Sixteen merino crossbred ram lambs 6 to 8 weeks of age (13.81kg ± 1.97) were used in the study. Lambs were randomly allocated to 1 of 4 treatment groups: (1) Conscious EEG and surgical castration with no anaesthetic intervention (CON; n = 4); (2) Conscious EEG and surgical castration with pre-operative applied intra-testicular lignocaine injection (CON + LIG; n = 4); (3) surgical castration under minimal anaesthesia (MAM; n = 4); (4) and surgical castration with pre-operative lignocaine injection (2 mL lignocaine hydrochloride 20 mg/mL, under minimal anaesthesia (MAM + LIG; n = 4). Distinct differences in the EEG parameters Ptot, F50 and F95 between pre-and post-castration in conscious lambs were demonstrated in this study (p < 0.01). Further, CON and CON + LIG treatments were distinguishable using F50 and F95 measures (p = 0.02, p = 0.04, respectively). Significant changes in the EEG output of MAM animals were identified pre- to post-castration (p < 0.01). The EEG output of MAM and MAM + LIG were similar. EEG was successful in differentiating lambs treated with pain relief in a conscious state after castration by examining F50 and F95, which may suggest the suitability of conscious EEG pain measurement.

scholarly journals Electroencephalographic Changes Associated with Antinociceptive Actions of Lidocaine, Ketamine, Meloxicam, and Morphine Administration in Minimally Anaesthetized Dogs

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Ubedullah Kaka ◽  
Chen Hui Cheng ◽  
Goh Yong Meng ◽  
Sharida Fakurazi ◽  
Asmatullah Kaka ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Postoperative Pain Management ◽  
Crossover Design ◽  
Median Frequency ◽  
Electric Stimulus ◽  
Treatment Groups ◽  
Morphine Administration ◽  
Antinociceptive Effects ◽  
Eeg Changes ◽  
Rate Infusion

Effects of ketamine and lidocaine on electroencephalographic (EEG) changes were evaluated in minimally anaesthetized dogs, subjected to electric stimulus. Six dogs were subjected to six treatments in a crossover design with a washout period of one week. Dogs were subjected to intravenous boluses of lidocaine 2 mg/kg, ketamine 3 mg/kg, meloxicam 0.2 mg/kg, morphine 0.2 mg/kg and loading doses of lidocaine 2 mg/kg followed by continuous rate infusion (CRI) of 50 and 100 mcg/kg/min, and ketamine 3 mg/kg followed by CRI of 10 and 50 mcg/kg/min. Electroencephalogram was recorded during electrical stimulation prior to any drug treatment (before treatment) and during electrical stimulation following treatment with the drugs (after treatment) under anaesthesia. Anaesthesia was induced with propofol and maintained with halothane at a stable concentration between 0.85 and 0.95%. Pretreatment median frequency was evidently increased (P<0.05) for all treatment groups. Lidocaine, ketamine, and morphine depressed the median frequency resulting from the posttreatment stimulation. The depression of median frequency suggested evident antinociceptive effects of these treatments in dogs. It is therefore concluded that lidocaine and ketamine can be used in the analgesic protocol for the postoperative pain management in dogs.

scholarly journals 83 The acute effects of feeding a grain-free diet with or without methionine, taurine or methyl donor/acceptor supplementation on plasma and whole blood amino acid concentrations in dogs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 62-62
Author(s):  
Sydney Banton ◽  
Julia G Pezzali ◽  
Renan Antunes Donadelli ◽  
Marica Bakovic ◽  
Katharine M Wood ◽  
...  
Keyword(s):  
Whole Blood ◽  
Repeated Measures ◽  
Latin Square ◽  
Acute Effects ◽  
Treatment Groups ◽  
Time Points ◽  
Postprandial Response ◽  
Donor Acceptor ◽  
Amino Acid Concentrations ◽  
Time Point

Abstract Grain-free pet foods have been sold for over a decade and comprise more than 40% of dog and cat diets sold today. Grain-free diets replace grain ingredients with pulse ingredients, which are high in lysine but low in methionine and cysteine, the precursor amino acids to taurine synthesis in the dog. The objective of this study was to evaluate the postprandial response of plasma methionine and taurine and whole blood taurine concentrations of dogs fed a grain-free diet without supplementation (CON) or with methionine (MET), taurine (TAU) or creatine, carnitine and choline (CCC) supplementation. Eight Beagles were pair housed and fed one of the four experimental diets for seven days in a 4x4 Latin Square Design. On the morning of d 7, cephalic catheters were placed and one fasted sample (0 min) and nine post-meal blood samples (15, 30, 60, 90, 120, 180, 240, 300 and 360 min) were collected. Data were analyzed as repeated measures using the PROC GLIMMIX function in SAS (Version 9.4). Dogs supplemented with MET had significantly higher plasma methionine concentrations from 30 to 360 minutes post-meal compared to dogs on CON, TAU and CCC treatments (P &lt; 0.05). However, no differences were observed in plasma methionine concentrations between CON, TAU and CCC treatments at any time point (P &gt; 0.05). Plasma taurine concentrations were significantly higher across time points in all treatment groups compared to CON (P &lt; 0.05). Whole blood taurine concentrations tended to be higher across time points in MET and TAU treatment groups compared to CCC (P = 0.0513). Overall, MET, TAU and CCC supplementation increased plasma taurine concentrations compared to CON, but only MET supplementation increased plasma methionine concentrations from 30 to 360 minutes post-meal.

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