scholarly journals Effects of Saccharides Supplementation in the Extender of Cryopreserved Rooster (Gallus domesticus) Semen on the Fertility of Frozen/Thawed Spermatozoa

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 189
Author(s):  
Olga Stanishevskaya ◽  
Yulia Silyukova ◽  
Nikolai Pleshanov ◽  
Anton Kurochkin ◽  
Elena Fedorova ◽  
...  
Keyword(s):  
Functional State ◽  
Artificial Insemination ◽  
Genital Tract ◽  
Gallus Domesticus ◽  
Functional Safety ◽  
Fertility Rates ◽  
Experimental Media ◽  
Egg Fertility ◽  
Perivitelline Membrane

The aim of this study was to create balanced media for the cryopreservation of rooster semen in pellets to maintain the functional state of the sperm after thawing. Fructose was replaced by trehalose in experimental media in proportions of 10% (LCM-T10) and 20% (LCM-T20), while LCM was used as a control. After artificial insemination of the hens, the eggs were incubated (n = 400). To determine the functional safety of spermatozoa in the genital tract of hens after 5, 10, and 15 days from the last insemination, we used a method for assessing the interaction of sperm with the perivitelline membrane. Significantly higher rates of egg fertilization (82–86%) were obtained when using LCM-T10 and LCM-T20 compared to control (79%, p < 0.05). Egg fertility on the 5th day from the last insemination with the LCM-T20 diluent reached 100% versus 86% in the control; on the 10th day, the fertility rates were 55% versus 20%, respectively. The best results for fertility duration were obtained by freezing spermatozoa with LCM-T20 medium. The numbers of interaction points of spermatozoa with the perivitelline membrane were as follows: on the 5th day from the last insemination with LCM-T20—461.5 ± 11.5 holes/cm2 (LCM-control—13.7 ± 2.7 holes/cm2), p < 0.01; on the 10th day with LCM-T20—319.3 ± 12.9 holes/cm2 (LCM-control—14.9 ± 3.5 holes/cm2); and on the 15th day with LCM-T20—345.2 ± 11.1 holes/cm2 (LCM-control—0 holes/cm2). In conclusion, the use of trehalose in LCM diluent medium can increase the fertility of frozen/thawed sperm and the duration of their fertility in the genital tract of hens.

ПРЕРЫВИСТОЕ ОСВЕЩЕНИЕ ПОВЫШАЕТ ЭФФЕКТИВНОСТЬ ИСКУССТВЕННОГО ОСЕМЕНЕНИЯ КУР В КЛЕТКАХ

2019 ◽  
Keyword(s):  
Reproductive Performance ◽  
Artificial Insemination ◽  
Egg Production ◽  
Laying Hens ◽  
Electric Energy ◽  
Productive Performance ◽  
Feed Conversion ◽  
Scientific Center ◽  
Academy Of Sciences ◽  
Egg Fertility

Intermittent Lighting Improves the Efficiency of Artificial Insemination in Cage Housed Laying Hens Kavtarashvili A.Sh., Kolokolnikova T.N. Federal Scientific Center “All-Russian Research and Technological Poultry Institute” of Russian Academy of Sciences Omsk Agrarian Scientific Center Summary: The effects of different lighting regimes on the oviposition schedule, productive performance, and reproductive efficiency in cage housed laying hens of layer parental flock (Hisex White-R) were studied; the reasonable regime of artificial insemination (AI) under intermittent lighting is proposed. It was found that intermittent lighting regime 1L:4D:4L:1D:4L:10D compared to the constant lighting significantly alters oviposition schedule: under this regime 82.3% of all daily eggs were laid until 9 am (vs. 66.6% in control). This regime and AI at 10 am improved the productive and reproductive performance compared to control (constant lighting 16L:8D and AI at 12 am): mortality by 1.9%, egg production per initial hen by 3.8%, egg weight by 1.1%, percentage of eggs suitable for incubation by 1.9%, egg fertility by 0.9%, hatchability by 2.3%, hatch of chicks by 2.9%, feed conversion ratio (kg of feed per 10 eggs) by 5.3%, the expenses of electric energy for lighting (per 1000 eggs suitable for incubation) by 54.5%. Key words: INTERMITTENT LIGHTING, CAGE HOUSED LAYERS, ARTIFICIAL INSEMINATION (AI), OVIPOSITION SCHEDULE, AI TIMING, PRODUCTIVE PERFORMANCE, REPRODUCTIVE PERFORMANCE

scholarly journals Fertilisation rate obtained with frozen-thawed boar semen supplemented with rosmarinic acid using a single insemination timed according to vulvar skin temperature changes

2015 ◽  
Vol 63 (1) ◽  
pp. 100-109 ◽  
Author(s):  
Victoria Luño ◽  
Lydia Gil ◽  
Maite Olaciregui ◽  
Juan Grandía ◽  
Trinidad Ansó ◽  
...  
Keyword(s):  
Skin Temperature ◽  
Artificial Insemination ◽  
Rosmarinic Acid ◽  
Intrauterine Insemination ◽  
Fertility Rates ◽  
Temperature Changes ◽  
Progressive Motility ◽  
A Value ◽  
Fertilisation Rate ◽  
Boar Semen

Artificial insemination (AI) of sows with frozen-thawed semen usually results in lower pregnancy rates and litter sizes than the use of liquid preserved semen. The present study evaluated the effectiveness of vulvar skin temperature changes as a predictor of ovulation in sows and determined the fertility rates obtained after AI with frozen-thawed semen supplemented with rosmarinic acid (RA). Semen was collected from mature boars and cryopreserved in experimental extenders supplemented with or without 105 μM of RA. Multiparous sows were inseminated with a single dose of semen when vulvar skin temperature decreased to a value below 35 °C. Intrauterine insemination was performed using 1.5 × 109 spermatozoa. The sows were slaughtered 48 h after AI and the embryos and oocytes were recovered from the oviducts. Total and progressive motility, viability and acrosome integrity were significantly (P < 0.05) higher in RA-supplemented semen samples compared with the control. Fertilisation occurred in all sows inseminated in the study, although there were no significant differences between the experimental groups. Sows inseminated with RA-supplemented semen showed a slight increase in the number of embryos recovered as compared to sows inseminated with control semen. In conclusion, insemination according to vulvar skin temperature changes resulted in successful fertilisation in all sows, although supplementation of the freezing media with RA did not improve the fertilising ability of frozen-thawed boar sperm.

scholarly journals Morphologic aspects of the genital tract from Nellore cows undergoing gynecological screening to insertion in the fixed-time artificial insemination (TAI) program

2014 ◽  
Vol 31 (02) ◽  
pp. 118-122
Author(s):  
R. Mello ◽  
M. Mello ◽  
M. Abidu-Figueiredo ◽  
P. Scherer ◽  
H. Palhano
Keyword(s):  
Artificial Insemination ◽  
Genital Tract ◽  
Body Condition Score ◽  
Fixed Time ◽  
The Body ◽  
Morphological Aspects ◽  
Functional Aspects ◽  
Condition Score ◽  
The Right ◽  
Rectal Palpation

Abstract Introduction: This study aimed to evaluate the morphology of the genital tract from 98 Nellore cows by rectal palpation and combine them with the functional aspects for inclusion in a fixed-time artificial insemination (TAI) program. Methods: Were evaluated the ovaries, cervix and vulva, as well as the body condition score (BCS scale 1-5). Results: In relation to the ovaries, there were palpable structures found in 51 (17 CL and 34 FL) in the rights ovarian and 37 palpable structures (06 CL and 31 FL) in the left ovary. Asymmetry was found in 17.3% and uterine cervicitis in 20.4% of examined females. Changes were noted in vulva in 51.0% (40 to 10 papules and hyperemia) and the average of BCS was 3.15. On the basis of morphological aspects founded, 29 females with an average of BCS 2.7 and 01 with metritis were excluded and 07 (BCS-3, 5) inseminated immediately after the exam, with 61 included in the TAI program. These data support the conclusion that of all ovulations occurred, characterized by the presence of CL, most occurred in the right ovary (73.9%). The prevalence rate (51%) of vulvar aspects found, indicate a need for research of reproductive diseases in their flock. Conclusion: The BCS may impacts on the cyclicity and in the pregnancy rate of females included in the program.

Maintenance of Simulium Wilhelmia lineatum Meigen and Simulium erythrocephalum de Geer through successive generations in the laboratory

1984 ◽  
Vol 62 (5) ◽  
pp. 870-877 ◽  
Author(s):  
P. J. Ham ◽  
A. E. Bianco
Keyword(s):  
Egg Production ◽  
Blood Feeding ◽  
Water Systems ◽  
Feeding Rates ◽  
Fertility Rates ◽  
Bovine Blood ◽  
Circulating Water ◽  
Development Period ◽  
Successive Generations ◽  
Egg Fertility

With the use of circulating water systems to rear larvae, Simulium erythrocephalum and S. lineatum have been continuously maintained through to the F7 and F3 generations, respectively. Insemination rates of 100% were obtained for both species by the F2 generation. Using chick skin membranes and bovine blood, mean blood-feeding rates of 56 and 87% were achieved for S. lineatum and S. erythrocephalum, respectively. Mean egg production and egg fertility rates increased after the parental generation was produced. Mating trials were peformed to assess conditions for optimum insemination rates. A significant correlation between temperature and complete generation development period was observed.

The role of the male cloacal gland in reproductive success in Japanese quail (Coturnix japonica)

2012 ◽  
Vol 24 (2) ◽  
pp. 405 ◽  
Author(s):  
R. P. Singh ◽  
K. V. H. Sastry ◽  
N. K. Pandey ◽  
K. B. Singh ◽  
I. A. Malecki ◽  
...  
Keyword(s):  
Japanese Quail ◽  
Normal Saline ◽  
Artificial Insemination ◽  
Coturnix Japonica ◽  
Fertile Period ◽  
Sperm Transport ◽  
Cloacal Gland ◽  
White Foam ◽  
Perivitelline Membrane

The adult male Japanese quail has a well developed cloacal gland that produces meringue-like white foam. The physiological significance of the cloacal gland and its foam is still unclear. Therefore, we conducted two experiments to establish the functional role of the cloacal gland and its foam in natural mating and oviducal sperm transport. In the first experiment, artificial insemination of equal numbers of spermatozoa diluted in foam extract and normal saline once in a week were used to determine the role of foam in sperm transport in the female oviduct. After artificial insemination, eggs were collected to measure fertility, the duration of the fertile period, sperm holes and attached spermatozoa in the perivitelline membrane. Higher (P < 0.05) fertility and greater duration of the fertile period were observed when semen was inseminated along with foam extract compared with normal saline. Further, the sperm holes and trapped spermatozoa in the perivitelline membrane were also higher (P < 0.05) in the presence of foam extract. In the second experiment, two males with bigger and smaller cloacal gland areas were allowed to mate with a female. The mating attempts of males with larger cloacal gland were more successful (P < 0.05) than males with smaller cloacal glands. Our results indicated that cloacal foam improves sperm transport in the female oviduct and that males with larger cloacal gland areas are preferred during mating.

Influence of Seminal Quality and Bull on Fertility Rates in Fixed-Time Artificial Insemination Programs.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 628-628
Author(s):  
Jose Antonio DellAqua ◽  
Andre Maciel Crespilho ◽  
Camila Paula DellAqua-Freitas ◽  
Priscilla Guasti ◽  
Fabiola Soares Zahn ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Fertility Rates

Artificial Insemination (AI) by Raw Semen: its Advantages and Disadvantages in Deshi Chicken (Gallus domesticus)

2004 ◽  
Vol 3 (10) ◽  
pp. 662-663 ◽  
Author(s):  
S.K. Das . ◽  
G.N. Adhikary . ◽  
M.N. Islam . ◽  
B.K. Paul . ◽  
G.G. Das .
Keyword(s):  
Artificial Insemination ◽  
Gallus Domesticus ◽  
Advantages And Disadvantages
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