scholarly journals Genetic Diversity of Sweet Potato (Ipomoea batatas L. Lam) Germplasms Collected Worldwide Using Chloroplast SSR Markers

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 752 ◽  
Author(s):  
Kyung Jun Lee ◽  
Gi-An Lee ◽  
Jung-Ro Lee ◽  
Raveendar Sebastin ◽  
Myoung-Jae Shin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Germplasm Collection ◽  
Diversity Indices ◽  
Management Plan ◽  
Shannon Index ◽  
Efficient Management ◽  
The Usa ◽  
Chloroplast Ssr

Sweet potato (Ipomoea batatas L. Lam) is an important food crop widely cultivated in the world. In this study, nine chloroplast simple sequence repeat (cpSSR) markers were used to analyze the genetic diversity and relationships of 558 sweet potato accessions in the germplasm collection of the National Agrobiodiversity Center (NAC). Eight of the nine cpSSR showed polymorphisms, while Ibcp31 did not. The number of alleles per locus ranged from two to four. In general, the Shannon index for each cpSSR ranged from 0.280 to 1.123 and the diversity indices and unbiased diversity ranged from 0.148 to 0.626, and 0.210 to 0.627, respectively. Results of the median-joining network showed 33 chlorotypes in 558 sweet potato accessions. In factor analysis, 558 sweet potato accessions were divided into four clusters, with clusters I and II composed only of the sweet potato accessions from Korea, Japan, Taiwan, and the USA. The results of this study confirmed that the genetic diversity of the female parents of sweet potato accessions conserved at the NAC is low and therefore more sweet potato accessions need to be collected. These results will help to establish an efficient management plan for sweet potato genetic germplasms at the NAC.

Analysis of genetic diversity in a sweet potato (Ipomoea batatas L.) germplasm collection from Tanzania as revealed by AFLP

2007 ◽  
Vol 55 (3) ◽  
pp. 397-408 ◽  
Author(s):  
Abdelhameed Elameen ◽  
Siri Fjellheim ◽  
Arild Larsen ◽  
Odd Arne Rognli ◽  
Leif Sundheim ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Germplasm Collection

scholarly journals GENETIC DIVERSITY AMONG SWEET POTATO CROPS CULTIVATED BY TRADITIONAL FARMERS

2018 ◽  
Vol 31 (3) ◽  
pp. 779-790 ◽  
Author(s):  
PABLO FORLAN VARGAS ◽  
ERIC WATZKE ENGELKING ◽  
LUIS CARLOS FERREIRA DE ALMEIDA ◽  
ELIEL ALVES FERREIRA ◽  
HAMILTON CESAR DE OLIVERIA CHARLO
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Block Design ◽  
Genetic Material ◽  
Morphological Characteristics ◽  
Leaf Size ◽  
Stem Length ◽  
Phenotypic Correlation ◽  
Crop Species

ABSTRACT The genetic variability within the crop species Ipomoea batatas is broad, hence, in order to support future breeding programs it is of the utmost importance that germplasm banks be created, conserved, and characterized. Therefore, the objective of this work was to rescue and evaluate the genetic divergence in sweet potato accessions collected in traditional communities of Vale do Ribeira Paulista. Sweet potato samples were collected from quilombos, indigenous villages, caiçaras communities, and small farms. The study was conducted between February 2013 and August 2014 in a randomized block design with three replications. Genetic material included 95 collected accessions and two commercial cultivars. Morphological characteristics of the accessions were evaluated and distances in the genetic distance matrix were estimated by means of multi-category variables, the data being subsequently clustered by the Tocher method. Analysis of the relative contribution of each characteristic and phenotypic correlation of descriptors was also performed. Results evidenced wide genetic diversity among the sweet potato accessions collected in Vale do Ribeira, which were not grouped according to the collection point. The descriptors that contributed more than 60% of genetic diversity included: leaf size, general leaf profile, immature leaf color, petiole pigmentation, predominant branch color, branch secondary color, stem length, cortical thickness, predominant periderm color, and periderm color intensity. Correlations between morphological descriptors was observed in 22.26% of the paired traits.

scholarly journals Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers

2008 ◽  
Vol 31 (3) ◽  
pp. 725-733 ◽  
Author(s):  
Elizabeth Ann Veasey ◽  
Aline Borges ◽  
Mariana Silva Rosa ◽  
Jurema R. Queiroz-Silva ◽  
Eduardo de Andrade Bressan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Sweet Potato ◽  
Ipomoea Batatas

scholarly journals Viral diagnosis in cultivars of Ipomoea batatas (L.) Lam.

2021 ◽  
Vol 49 (1) ◽  
pp. 12222
Author(s):  
Carlos G. ANDRADE ◽  
Emanuel M. Da SILVA ◽  
Carla RAGONEZI ◽  
Miguel Â. A. PINHEIRO DE CARVALHO
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Viral Infections ◽  
Catalytic Domain ◽  
Sequence Similarity ◽  
Vegetative Reproduction ◽  
Germplasm Collection ◽  
Probability Method ◽  
Infection Prevalence ◽  
Sequence Identity

Ipomoea batatas (L.) Lam. commonly known as sweet potato, is an important staple food worldwide, mainly due to its high nutritional value and yield. However, vegetative reproduction of sweet potato makes it more susceptible to viral infections, which threatens its productivity, quality, and difficult long-term preservation in germplasm banks. Also, it can act as a virus reservoir infecting the rest of the plant accessions in the bank collections. Hence, this work aimed to screen Begomovirus, Potyvirus, and Carlavirus infections in 16 traditional sweet potato cultivars from the germplasm collection of the ISOPlexis Germplasm Bank, Madeira, Portugal. The infection prevalence by these viruses among cultivars was 81.25%, 25.00%, and 6.25%, respectively; being ISOP1011 the only accession coinfected by Potyvirus and Carlavirus. The accessions ISOP1006, ISOP1010, and ISOP1047 were also coinfected by Begomovirus and Potyvirus, highlighting their vulnerability to viral infections. The ISOP1005 and ISOP1027 accessions were the only ones not infected by any of these viruses. The analysis of the partial sequence obtained from the Carlavirus detected in the accession ISOP1011, revealed the existence of an ORF that encodes for 93 amino acids of the catalytic domain of an RNA-directed RNA polymerase related to the Tymovirus protein family, as could be confirmed by comparison with proteins stored in UniProtKB. Multiple sequence alignment with these proteins showed that Motifs A and B of the catalytic domain were conserved. The search for sequence similarity with sequences deposited in GenBank reported a high sequence identity with Sweet potato yellow mottle virus (SPYMV) and Sweet potato chlorotic fleck virus (SPCFV). However, the 9-11% discrepancy in nucleotide sequence identity and a phylogenetic analysis carried out using the maximum probability method suggests the virus isolated from ISOP1011 is a new divergent strain of the SPCFV species.

Genetic diversity analysis of sweet potato [Ipomoea batatas (L.) Lam.] germplasms through RAPD and ISSR markers

2021 ◽  
Vol 50 (1) ◽  
pp. 119-129
Author(s):  
PN Narasimha Murthy ◽  
NB Patel ◽  
Chintan Kapadia ◽  
KD Desai ◽  
G Koteswara Rao
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Genetic Relatedness ◽  
Issr Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Polymorphic Loci ◽  
Improvement Programme ◽  
Rapd And Issr

Sweet potato [Ipomoea batatas (L.) Lam.] germplasms are unique as they are heterozygous, vegitatively as well as sexually propagated and polyploid in nature. Forty-four germplasms from local farmer’s field and from various centres of India were determined using 15 RAPD and 15 ISSR markers to evaluate their genetic relatedness. Average polymorphisms for RAPD and ISSR markers were found to be 86.72 and 81.64%, respectively. The OPM series primer produced 145 total bands with 138 polymorphic loci and ISSR markers gave 116 total bands with 101 polymorphic loci. The clusters were made using the UPGMA method using both the RAPD and ISSR data and group diverse yet similar germplasm in two different clusters. This gives ample information to use them in further improvement programme.

scholarly journals On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity

Heredity ◽  
2013 ◽  
Vol 110 (6) ◽  
pp. 594-604 ◽  
Author(s):  
C Roullier ◽  
R Kambouo ◽  
J Paofa ◽  
D McKey ◽  
V Lebot
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
New Guinea ◽  
Centre Of Diversity

scholarly journals Evaluation of genetic variability among “Early Mature”Juglans regiausing microsatellite markers and morphological traits

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3834 ◽  
Author(s):  
Aziz Ebrahimi ◽  
Abdolkarim Zarei ◽  
Mojtaba Zamani Fardadonbeh ◽  
Shaneka Lawson
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Normal Growth ◽  
Diversity Indices ◽  
Shannon Index ◽  
Phenotypic Trait ◽  
Phenotypic Traits ◽  
High Genetic Diversity ◽  
Early Maturation ◽  
Persian Walnut

Limiting the juvenile phase and reducing tree size are the two main challenges for breeders to improve most fruit crops. Early maturation and dwarf cultivars have been reported for many fruit species. “Early mature” and low vigor walnut genotypes were found among seedlings of Persian walnut. Nine microsatellite markers were used to evaluate genetic diversity among “Early Mature” Persian walnut accessions and provide a comparison with “normal growth” accessions. Six maturation related characteristics were also measured in “Early Mature” samples. Phenotypic traits and diversity indices showed relatively high levels of genetic diversity in “Early Mature” seedlings and indicated high differentiation between individuals. Seedling height, the most diverse phenotypic trait, has an important role in the clustering of “Early Mature” accessions. The “Early Mature” type had higher number of alleles, number of effective allele, and Shannon index compared to the “Normal Growth” group. The two types of studied walnuts had different alleles, with more than half of produced alleles specific to a specific group. “Early Mature” and “Normal Growth” walnuts had 27 and 17 private alleles, respectively. Grouping with different methods separated “Early Mature” and “Normal Growth” samples entirely. The presence of moderate to high genetic diversity in “Early Mature” walnuts and high genetic differentiation with “Normal Growth” walnuts, indicated that “Early Mature” walnuts were more diverse and distinct from “Normal Growth” samples. Moreover, our results showed SSR markers were useful for differentiating between “Early Mature” and “Normal Growth” walnuts. A number of identified loci have potential in breeding programs for identification of “Early Mature” walnuts at the germination phase.

scholarly journals Studies on Genetic Diversity for Yield, Growth and Quality Traits in Sweet Potato [Ipomoea batatas (L.) Lam.]

2017 ◽  
Vol 4 (4) ◽  
pp. 113-117
Author(s):  
Devesh Pratap Singh ◽  
Chandra Deo ◽  
Deepak Kumar Gautam ◽  
Rajesh Kumar ◽  
Pushpendra Kumar
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Quality Traits

Development of chloroplast microsatellite markers for identification of Glycyrrhiza species

2018 ◽  
Vol 17 (1) ◽  
pp. 95-98 ◽  
Author(s):  
Kyung Jun Lee ◽  
Sebastin Raveendar ◽  
Ji Seon Choi ◽  
Jinsu Gil ◽  
Jeong Hoon Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Traditional Medicine ◽  
Chloroplast Genome ◽  
Diversity Indices ◽  
Rapid Identification ◽  
Shannon Index ◽  
Medicinal Herbs ◽  
Medicinal Herb ◽  
Chloroplast Microsatellite

AbstractLicorice (Glycyrrhiza glabra) is an important medicinal herb and has long been used in traditional medicine for the treatment of several diseases worldwide. Understanding the genetic diversity within Glycyrrhiza species is important for the efficient conservation of these medicinal herbs. In this study, we have developed 20 polymorphic chloroplast microsatellite (cpSSR) markers using the chloroplast genome of G. lepidota. The cpSSR markers were tested on a total of 27 Glycyrrhiza individual plants. The number of alleles per locus ranged from two to eight among the Glycyrrhiza accessions. Overall, the Shannon index (I) for each cpSSR ranged from 0.315 to 1.694, the diversity indices (h) were 0.140–0.793 and the unbiased diversity indices (uh) were 0.145–0.825. In addition, the cpSSR markers were successfully divided and classified the 27 Glycyrrhiza individuals into four groups. The cpSSR markers developed in this study could be used in the assessment of genetic diversity and rapid identification of Glycyrrhiza species.

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