scholarly journals Viral diagnosis in cultivars of Ipomoea batatas (L.) Lam.

2021 ◽  
Vol 49 (1) ◽  
pp. 12222
Author(s):  
Carlos G. ANDRADE ◽  
Emanuel M. Da SILVA ◽  
Carla RAGONEZI ◽  
Miguel Â. A. PINHEIRO DE CARVALHO
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Viral Infections ◽  
Catalytic Domain ◽  
Sequence Similarity ◽  
Vegetative Reproduction ◽  
Germplasm Collection ◽  
Probability Method ◽  
Infection Prevalence ◽  
Sequence Identity

Ipomoea batatas (L.) Lam. commonly known as sweet potato, is an important staple food worldwide, mainly due to its high nutritional value and yield. However, vegetative reproduction of sweet potato makes it more susceptible to viral infections, which threatens its productivity, quality, and difficult long-term preservation in germplasm banks. Also, it can act as a virus reservoir infecting the rest of the plant accessions in the bank collections. Hence, this work aimed to screen Begomovirus, Potyvirus, and Carlavirus infections in 16 traditional sweet potato cultivars from the germplasm collection of the ISOPlexis Germplasm Bank, Madeira, Portugal. The infection prevalence by these viruses among cultivars was 81.25%, 25.00%, and 6.25%, respectively; being ISOP1011 the only accession coinfected by Potyvirus and Carlavirus. The accessions ISOP1006, ISOP1010, and ISOP1047 were also coinfected by Begomovirus and Potyvirus, highlighting their vulnerability to viral infections. The ISOP1005 and ISOP1027 accessions were the only ones not infected by any of these viruses. The analysis of the partial sequence obtained from the Carlavirus detected in the accession ISOP1011, revealed the existence of an ORF that encodes for 93 amino acids of the catalytic domain of an RNA-directed RNA polymerase related to the Tymovirus protein family, as could be confirmed by comparison with proteins stored in UniProtKB. Multiple sequence alignment with these proteins showed that Motifs A and B of the catalytic domain were conserved. The search for sequence similarity with sequences deposited in GenBank reported a high sequence identity with Sweet potato yellow mottle virus (SPYMV) and Sweet potato chlorotic fleck virus (SPCFV). However, the 9-11% discrepancy in nucleotide sequence identity and a phylogenetic analysis carried out using the maximum probability method suggests the virus isolated from ISOP1011 is a new divergent strain of the SPCFV species.

Analysis of genetic diversity in a sweet potato (Ipomoea batatas L.) germplasm collection from Tanzania as revealed by AFLP

2007 ◽  
Vol 55 (3) ◽  
pp. 397-408 ◽  
Author(s):  
Abdelhameed Elameen ◽  
Siri Fjellheim ◽  
Arild Larsen ◽  
Odd Arne Rognli ◽  
Leif Sundheim ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Germplasm Collection

scholarly journals Occurrence of Sweet potato feathery mottle virus and Sweet potato leaf curl Georgia virus on Sweet Potato in India

Plant Disease ◽  
2008 ◽  
Vol 92 (2) ◽  
pp. 311-311 ◽  
Author(s):  
G. Prasanth ◽  
V. Hegde
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Sequence Similarity ◽  
Disease Incidence ◽  
Alignment Score ◽  
Mottle Virus ◽  
Potato Leaf ◽  
Vein Clearing ◽  
Query Coverage ◽  
Leaf Curl

Viruses are a major biotic constraint on sweet potato (Ipomoea batatas (L.) Lam) production worldwide. In 2005, 10 to 60% viral disease incidence was observed in sweet potato fields. Symptoms include ring and chlorotic spots, puckering, feathering, vein clearing, and leaf curl with chlorotic specks and pink spots. Cuttings from symptomatic plants were collected from Kerala (two clones), Orrisa (eight clones), and Adrapradesh (three clones) and maintained in an insect-proof glasshouse. Leaves from symptomatic plants were mechanically inoculated to I setosa, I. nil, Nicotiana tabacum, N. benthamiana, Datura stramonium, and Chenapodium quinoa (12 seedlings each). Vein clearing, netting, and leaf distortion were observed in I. setosa and N tabacum 7 days postinoculation, chlorotic spots observed in N. benthamiana, and violet spots and violet margins on leaves observed on I. Nil. No symptoms were observed on D. stramonium and C. quinoa. When scions from the symptomatic sweet potato plants were graft inoculated onto I. setosa, vein clearing, leaf curl, and puckering-like symptoms were observed within 5 days. Mosaic and leaf curling symptoms were also observed on mechanically inoculated N. tabacum. Total nucleic acids isolated from the 33 field-collected sweet potato samples, graft inoculated I. setosa plants, and mechanically inoculated N. tabacum and I. nil plants were used for PCR and reverse transcription (RT)-PCR with geminivirus group specific (2) and potyvirus group specific primers (1). The expected 530-bp and 1.3-kb fragment were generated from the geminivirus and potyvirus primer sets, respectively. Potyvirus alone was detected in 7 of the 33 field-collected plants; geminivirus alone was detected in 7 other plants, while 19 plants contained detectible levels of potyvirus and geminivirus. To further identify the viruses, nested primers specific for the coat protein gene of Sweet potato feathery mottle virus (SPFMV) (CP1S 5′AGT GGG AAG GCA CCA TAC ATA GC 3′, CP1A5′ GCA GAG GAT GTC CTA TTG CAC ACC 3′) (CP2S 5′TCT AGT GAA CGT ACT GAA TTC AAA GA 3′, CP2A 5′ATT GCA CAC CCC TGA TTC CTA AGA 3′) and Sweet potato leaf curl virus (SPLCV) (CP1- 5′ATG ACA GGG CGA ATT CGC GTT TC 3′, CP2- 5′TTA ATT TTT GTG CGA ATC ATA 3′) were designed. I. setosa and N. tabacum were amplified with SPFMV and SPLCV primers and the amplicons of 960 and 764 bp, respectively, obtained were subsequently cloned into pGEM-T Easy vector and sequenced. Nucleotide BLAST analysis revealed that the 960-bp fragment (GenBank Accession No. EF015398.) was 98% identical to two Egyptian isolates of SPFMV (Nos. AJ 515379 and AJ 515378). The nucleotide sequence of the 764-bp products (Nos. EF 151926 and EF15483) from the samples collected from Kerala and Orisa was 95% identical to each other. The sequence identity of EF 15483 with Sweet potato leaf curl Georgia virus (SPLCGV) isolate AF326775. was 91% and identity with China isolate DQ 512731 was 90% The isolate EF 151926 also was 91% identical to the SPLCGV with a high query and alignment score whereas identity with the China isolate was 91% with a low query coverage and alignment score. Phylogenic analysis with MEGA software program also showed the highest sequence similarity with SPLCGV, hence it is concluded that the geminivirus isolate under study is SPLCGV. To our knowledge, this is the first report of identification of SPFMV and SPLCGV occurring on sweet potato in India. Further study is required to understand the consequences of occurrence of these two viruses in India. References: (1) D. Colinet et al. Plant Dis. 28:223 1998. (2) D. D. Deng et al. Ann. Appl. Biol 125:327, 1993.

scholarly journals ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China

2014 ◽  
Vol 13 (11) ◽  
pp. 2346-2361 ◽  
Author(s):  
Kai ZHANG ◽  
Zheng-dan WU ◽  
Yan-hua LI ◽  
Han ZHANG ◽  
Liang-ping WANG ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Germplasm Collection ◽  
Elite Germplasm

scholarly journals Genetic Diversity of Sweet Potato (Ipomoea batatas L. Lam) Germplasms Collected Worldwide Using Chloroplast SSR Markers

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 752 ◽  
Author(s):  
Kyung Jun Lee ◽  
Gi-An Lee ◽  
Jung-Ro Lee ◽  
Raveendar Sebastin ◽  
Myoung-Jae Shin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Germplasm Collection ◽  
Diversity Indices ◽  
Management Plan ◽  
Shannon Index ◽  
Efficient Management ◽  
The Usa ◽  
Chloroplast Ssr

Sweet potato (Ipomoea batatas L. Lam) is an important food crop widely cultivated in the world. In this study, nine chloroplast simple sequence repeat (cpSSR) markers were used to analyze the genetic diversity and relationships of 558 sweet potato accessions in the germplasm collection of the National Agrobiodiversity Center (NAC). Eight of the nine cpSSR showed polymorphisms, while Ibcp31 did not. The number of alleles per locus ranged from two to four. In general, the Shannon index for each cpSSR ranged from 0.280 to 1.123 and the diversity indices and unbiased diversity ranged from 0.148 to 0.626, and 0.210 to 0.627, respectively. Results of the median-joining network showed 33 chlorotypes in 558 sweet potato accessions. In factor analysis, 558 sweet potato accessions were divided into four clusters, with clusters I and II composed only of the sweet potato accessions from Korea, Japan, Taiwan, and the USA. The results of this study confirmed that the genetic diversity of the female parents of sweet potato accessions conserved at the NAC is low and therefore more sweet potato accessions need to be collected. These results will help to establish an efficient management plan for sweet potato genetic germplasms at the NAC.

Phenotypic diversity of plant morphological and root descriptor traits within a sweet potato, Ipomoea batatas (L.) Lam., germplasm collection from Tanzania

2010 ◽  
Vol 58 (3) ◽  
pp. 397-407 ◽  
Author(s):  
Abdelhameed Elameen ◽  
Arild Larsen ◽  
Sonja S. Klemsdal ◽  
Siri Fjellheim ◽  
Leif Sundheim ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Phenotypic Diversity ◽  
Germplasm Collection

scholarly journals Properties of Strains of Sweet potato feathery mottle virus and Two Newly Recognized Potyviruses Infecting Sweet Potato in the United States

Plant Disease ◽  
2003 ◽  
Vol 87 (10) ◽  
pp. 1226-1232 ◽  
Author(s):  
E. R. Souto ◽  
J. Sim ◽  
J. Chen ◽  
R. A. Valverde ◽  
C. A. Clark
Keyword(s):  
United States ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Sequence Similarity ◽  
Aphis Gossypii ◽  
The United States ◽  
Biological Properties ◽  
Mottle Virus ◽  
High Sequence Similarity ◽  
High Degree

Some biological and molecular properties of six potyvirus isolates (LSU-1, -2, -3, and -5; 95-2; and 95-6) from sweet potato (Ipomoea batatas) were evaluated. Isolates LSU-1 and -3 and 95-2 were transmitted by Aphis gossypii and Myzus persicae while LSU-2 and -5 were not transmitted by either aphid. The partial nucleotide sequence of the nuclear inclusion b (NIb) and the coat protein (CP) genes of these six isolates were compared with the corresponding sequences of 17 Sweet potato feathery mottle virus (SPFMV) strains and 18 other potyviruses. LSU-1 and -3 had high sequence similarity to the published sequences for Sweet potato virus G (SPVG), did not react with antisera to other known sweet potato viruses, and caused distinct symptoms. We propose to designate these two isolates as SPVG. This report documents the occurrence of this virus in the United States and provides the first characterization of its biological properties. LSU-2 and -5 were distinct in symptomatology; partial Nib, CP nucleotide, and derived amino acid sequence; and serology to other viruses. We propose to call this virus (LSU isolates 2 and 5) Ipomoea vein mosaic virus. The present study revealed a high degree of sequence similarity between 95-6 and the common strain of SPFMV, and between 95-2 and the russet crack strain of SPFMV. Results from this study suggest not only that at least two strains of SPFMV occur in the United States, but that two other potyviruses also are present.

scholarly journals Novel begomovirus species of recombinant nature in sweet potato (Ipomoea batatas) and Ipomoea indica: taxonomic and phylogenetic implications

2009 ◽  
Vol 90 (10) ◽  
pp. 2550-2562 ◽  
Author(s):  
Gloria Lozano ◽  
Helena P. Trenado ◽  
Rodrigo A. Valverde ◽  
Jesús Navas-Castillo
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Viral Infections ◽  
Begomovirus Species ◽  
Virus Species ◽  
Potato Leaf ◽  
New Horizons ◽  
Phylogenetic Implications ◽  
Phylogeny And Evolution ◽  
Leaf Curl Virus

Viral diseases occur wherever sweet potato (Ipomoea batatas) is cultivated and because this crop is vegetatively propagated, accumulation and perpetuation of viruses can become a major constraint for production. Up to 90 % reductions in yield have been reported in association with viral infections. About 20 officially accepted or tentative virus species have been found in sweet potato and other Ipomoea species. They include three species of begomoviruses (genus Begomovirus, family Geminiviridae) whose genomes have been fully sequenced. In this investigation, we conducted a search for begomoviruses infecting sweet potato and Ipomoea indica in Spain and characterized the complete genome of 15 isolates. In addition to sweet potato leaf curl virus (SPLCV) and Ipomoea yellowing vein virus, we identified three new begomovirus species and a novel strain of SPLCV. Our analysis also demonstrated that extensive recombination events have shaped the populations of Ipomoea-infecting begomoviruses in Spain. The increased complexity of the unique Ipomoea-infecting begomovirus group, highlighted by our results, open new horizons to understand the phylogeny and evolution of the family Geminiviridae.

Batatins VII-X, glycolipid ester-type dimers from sweet potato (Ipomoea batatas)

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
D Rosas-Ramírez ◽  
R Pereda-Miranda
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

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