scholarly journals Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B9 Content in Potato Tubers

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 734 ◽  
Author(s):  
Bruce R. Robinson ◽  
Carolina Garcia Salinas ◽  
Perla Ramos Parra ◽  
John Bamberg ◽  
Rocio I. Diaz de la Garza ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Tomato Fruit ◽  
Genetically Engineered ◽  
Food Crops ◽  
Great Success ◽  
Rice Grain ◽  
Rna Seq ◽  
Potato Tubers ◽  
Vitamin B9 ◽  
Folate Biosynthesis

Biofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and lack of economic model have prevented the widespread adoption of nutritionally-enhanced genetically-engineered (GE) food crops. Meanwhile, little effort has been made to biofortify food crops with folate by breeding. Previously we reported >10-fold variation in folate content in potato genotypes. To facilitate breeding for enhanced folate content, we attempted to identify genes that control folate content in potato tuber. For this, we analyzed the expression of folate biosynthesis and salvage genes in low- and high-folate potato genotypes. First, RNA-Seq analysis showed that, amongst all folate biosynthesis and salvage genes analyzed, only one gene, which encodes γ-glutamyl hydrolase 1 (GGH1), was consistently expressed at higher levels in high- compared to low-folate segregants of a Solanum boliviense Dunal accession. Second, quantitative PCR showed that GGH1 transcript levels were higher in high- compared to low-folate segregants for seven out of eight pairs of folate segregants analyzed. These results suggest that GGH1 gene expression is an indicator of folate content in potato tubers.

scholarly journals BIOLOGY AND RESISTANCE OF PHYTOPATHOGENIC FUNGUS ILYONECTRIA CRASSA TO FUNGICIDES

2020 ◽  
Vol 103 (3) ◽  
pp. 196-201
Author(s):  
E. M. Chudinova ◽  
V. A. Platonov ◽  
A. V. Alexandrova ◽  
S. N. Elansky
Keyword(s):  
Potato Tuber ◽  
Tomato Fruit ◽  
Phytopathogenic Fungus ◽  
Potato Tubers ◽  
Protective Measures ◽  
Protein Coding ◽  
Disease Induction ◽  
Infected Tomato ◽  
Species Specific ◽  
First Time

It has been recently shown that the ascomycete fungus Ilyonectria crassa can infect potato tubers. In this work, the biological characteristics and resistance to some fungicides of the “potato” I. crassa strain were analyzed for the first time. The sequences of the species-specific regions of the tested strain (rRNA and protein-coding genes) were identical to those obtained earlier for the fungi isolated from the roots of narcissus, ginseng, aspen, beech, as well as lily bulbs and tulip leaves. Apparently, many wild and garden plants can be carriers of I. crassa. The investigated strain infected tomato and potato slices but did not infect the whole intact tomato fruit and potato tuber. This demonstrated that I. crassa is a wound pathogen. Evaluation of resistance to fludioxonil, difenoconazole and azoxystrobin on a nutrient medium showed high efficacy of these compounds. The EC50 index, i.e. the concentration of the fungicide slowing the rate of radial growth of the colony by 2 times as compared to the non-fungicidal control, was equal to 0.4; 7.4 and 4 mg/L, respectively. The possibility of disease induction by I. crassa should be considered for evaluation of potato tuber infections and development of protective measures.

scholarly journals Targeting HSPA1A in ARID2-deficient lung adenocarcinoma

2021 ◽  
Author(s):  
Xue Wang ◽  
Yuetong Wang ◽  
Zhaoyuan Fang ◽  
Hua Wang ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Adenocarcinoma ◽  
Genetically Engineered ◽  
Malignant Progression ◽  
Murine Models ◽  
Rna Seq ◽  
Potential Therapeutic Target ◽  
Lung Cancers ◽  
Integrative Analyses

Abstract Somatic mutations of the chromatin remodeling gene ARID2 are observed in about 7% of human lung adenocarcinoma (LUAD). However, the role of ARID2 in the pathogenesis of LUAD remains largely unknown. Here we find that ARID2 expression is decreased during the malignant progression of both human and mice LUAD. Using two KrasG12D-based genetically engineered murine models (GEMM), we demonstrate that ARID2 knockout significantly promotes lung cancer malignant progression and shortens the overall survival. Consistently, ARID2 knockdown significantly promotes cell proliferation in human and mice lung cancer cells. Through integrative analyses of Chip-Seq and RNA-Seq data, we find that Hspa1a is up-regulated by Arid2 loss. Knockdown of Hspa1a specifically inhibits malignant progression of Arid2-deficient but not Arid2-wt lung cancers in both cell lines as well as animal models. Treatment with Hspa1a inhibitor could significantly inhibit the malignant progression of lung cancer with Arid2 deficiency. Together, our findings establish ARID2 as an important tumor suppressor in LUAD with novel mechanistic insights, and further identify HSPA1A as a potential therapeutic target in ARID2-deficient LUAD.

scholarly journals Buzz-Pollinated Crops: A Global Review and Meta-analysis of the Effects of Supplemental Bee Pollination in Tomato

2021 ◽  
Author(s):  
Hazel Cooley ◽  
Mario Vallejo-Marín
Keyword(s):  
Meta Analysis ◽  
Tomato Fruit ◽  
Economic Benefits ◽  
Fruit Weight ◽  
Food Crops ◽  
Bee Pollination ◽  
Solanum Lycopersicum L ◽  
Tomato Yield ◽  
Pollination Control ◽  
Supplemental Pollination

Abstract Buzz-pollinated plants require visitation from vibration producing bee species to elicit full pollen release. Several important food crops are buzz-pollinated including tomato, eggplant, kiwi, and blueberry. Although more than half of all bee species can buzz pollinate, the most commonly deployed supplemental pollinator, Apis mellifera L. (Hymenoptera: Apidae; honey bees), cannot produce vibrations to remove pollen. Here, we provide a list of buzz-pollinated food crops and discuss the extent to which they rely on pollination by vibration-producing bees. We then use the most commonly cultivated of these crops, the tomato, Solanum lycopersicum L. (Solanales: Solanaceae), as a case study to investigate the effect of different pollination treatments on aspects of fruit quality. Following a systematic review of the literature, we statistically analyzed 71 experiments from 24 studies across different geopolitical regions and conducted a meta-analysis on a subset of 21 of these experiments. Our results show that both supplemental pollination by buzz-pollinating bees and open pollination by assemblages of bees, which include buzz pollinators, significantly increase tomato fruit weight compared to a no-pollination control. In contrast, auxin treatment, artificial mechanical vibrations, or supplemental pollination by non-buzz-pollinating bees (including Apis spp.), do not significantly increase fruit weight. Finally, we compare strategies for providing bee pollination in tomato cultivation around the globe and highlight how using buzz-pollinating bees might improve tomato yield, particularly in some geographic regions. We conclude that employing native, wild buzz pollinators can deliver important economic benefits with reduced environmental risks and increased advantages for both developed and emerging economies.

scholarly journals Genetically engineered flavonol enriched tomato fruit modulates chondrogenesis to increase bone length in growing animals

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Dharmendra Choudhary ◽  
Ashutosh Pandey ◽  
Sulekha Adhikary ◽  
Naseer Ahmad ◽  
Chitra Bhatia ◽  
...  
Keyword(s):  
Tomato Fruit ◽  
Genetically Engineered ◽  
Bone Length

THE FLUORESCENCE OF FROZEN POTATO TUBER AND APPLE FRUIT TISSUE UNDER ULTRA-VIOLET LIGHT

1945 ◽  
Vol 23c (2) ◽  
pp. 76-78 ◽  
Author(s):  
W. Newton ◽  
W. Jones
Keyword(s):  
Potato Tuber ◽  
Ultra Violet ◽  
Apple Fruit ◽  
Potato Tubers ◽  
Tuber Tissue ◽  
Fruit Tissue ◽  
Potato Tuber Tissue ◽  
Violet Light ◽  
Apple Tissue ◽  
Acetone Extracts

Freshly cut sections of frozen potato tuber and apple fruit tissue exhibit a brilliant fluorescence when examined in a dark room under an ultra-violet (Stroblite) lamp. The fluorescence disappears from the apple tissue upon thawing but is retained in potato tuber tissue. Thus the ultra-violet lamp serves as a useful diagnostic means of detecting frost or low temperature injury in potato tubers. Many other plant tissues were frozen and examined, but proved to be non-fluorescent. Acetone extracts of both normal and frozen potato tuber tissue were fluorescent, but similar extracts of both frozen and normal apple fruit were not. Although normal potato tissue is non-fluorescent and frozen tissue is brightly fluorescent, no differences were found in the brightness of the acetone extracts.

Tomato fruit size, maturity and α-tomatine content influence the performance of larvae of potato tuber moth Phthorimaea operculella (Lepidoptera: Gelechiidae)

2006 ◽  
Vol 96 (2) ◽  
pp. 173-178 ◽  
Author(s):  
B. Mulatu ◽  
S.W. Applebaum ◽  
Z. Kerem ◽  
M. Coll
Keyword(s):  
Potato Tuber ◽  
Tomato Fruit ◽  
Fruit Size ◽  
Chemical Properties ◽  
Head Capsule ◽  
Phthorimaea Operculella ◽  
Larval Performance ◽  
Potato Tuber Moth ◽  
Fresh Market ◽  
Feeding Site

AbstractVarious physical and chemical properties of host plants influence insect larval performance and subsequent adult fitness. Tomato plants are relatively new hosts to the potato tuber moth, Phthorimaea operculella (Zeller), with the fruit being its preferred feeding site. However, it is unclear how the biochemical and physical properties of tomato fruits relate to potato tuber moth performance. Significant amounts of α-tomatine were detected in maturing green and ripening fruits of cherry (cv. Ceres) and processing (cv. Serio) types of tomatoes whereas none was detected in a fresh market variety (cv. Marglobe), at comparable stages. α-Tomatine is negatively and significantly correlated with development rate (head capsule size) of larvae reared in the fruits of the cherry and processing type tomatoes. Generally, survival, growth and development were significantly superior for larvae reared in the ripening fruits of the fresh market cultivar. At this stage, the fruits of this cultivar are also the largest. Based on these results it is concluded that fruit α-tomatine content, as wellas fruit size and maturity, all affect performance of P. operculella larvae in the fruits of cultivated tomatoes.

scholarly journals Variety-specificity of soluble proteins of potato tubers

2015 ◽  
Vol 43 (4) ◽  
pp. 491-498 ◽  
Author(s):  
Marta Czupryn ◽  
Kazimierz Toczko
Keyword(s):  
Potato Tuber ◽  
Genetic Research ◽  
Electrophoretic Pattern ◽  
Electrophoretic Analysis ◽  
Disc Electrophoresis ◽  
The Other ◽  
Potato Tubers ◽  
Genetic Origin ◽  
Electrophoretic Patterns ◽  
Taxonomic Studies

Separation of soluble tuber proteins from six potato clones and twelve varieties cultivated in Poland has been accomplished by disc electrophoresis. It was found that electrophoretic pattern was unique for a given clone or variety. Data obtained confirm results of the other authors for the other varieties and indicate that electrophoretic analysis of potato tuber proteins can be a useful method for taxonomic studies. Such analysis however cannot be used for genetic research since no correlations were found between electrophoretic patterns and genetic origin of respective clones and varieties.

scholarly journals Growth and yield of pomato under the Syrian coastal mountains conditions

2021 ◽  
Vol 13 (4) ◽  
pp. 382-388
Author(s):  
Alaa Suhiel Ibrahim
Keyword(s):  
Fruit Set ◽  
Tomato Fruit ◽  
Shape Index ◽  
The Other ◽  
Soluble Solids ◽  
Growth And Yield ◽  
Potato Tubers ◽  
Average Yield ◽  
Tomato Plants ◽  
Significant Superiority

Abstract. The world has always been striving to increase and intensify agricultural production, and there are several attempts to acheive that, such as grafting tomato on potato to obtain two crops from one plant. This investigation was conducted during 2020 in open field in Nahl village, Baniyas, Tartous Governorate, Syria. The vegetative and generative growth and yield have been studied for pomato (grafted tomato on potato) and compared to each tomato and potato separately. Tomato plants were significantly superior in plant height to pomato plants after 23 days of planting until the end of the experiment. On the other hand, there were significant differences between tomato plants and pomato plants in the leaf area after 23, 51 and 65 days of planting. Also, tomato plants significantly outperformed pomato plants in the number of flowers and fruits per plant and the tomato fruit set percent. Anyway, tomato plants were significantly superior in the average yield of tomato fruits per plant (1657 g.plant-1) to pomato plants (185 g.plant-1), while there were insignificant differences in the average yield of potato tubers per plant between pomato which failed to produce tubers and potato (48.25 g.plant-1). Studying the tomato fruit fresh weight showed a significant superiority of tomato plants (54.37 g.fruit-1) to pomato plants (35.97 g.fruit-1), while there were insignificant differences in the other physical (tomato fruit height, diameter and shape index) and chemical (tomato fruit content of total acids, total soluble solids and dry matter, %) properties.

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