THE FLUORESCENCE OF FROZEN POTATO TUBER AND APPLE FRUIT TISSUE UNDER ULTRA-VIOLET LIGHT

1945 ◽  
Vol 23c (2) ◽  
pp. 76-78 ◽  
Author(s):  
W. Newton ◽  
W. Jones

Freshly cut sections of frozen potato tuber and apple fruit tissue exhibit a brilliant fluorescence when examined in a dark room under an ultra-violet (Stroblite) lamp. The fluorescence disappears from the apple tissue upon thawing but is retained in potato tuber tissue. Thus the ultra-violet lamp serves as a useful diagnostic means of detecting frost or low temperature injury in potato tubers. Many other plant tissues were frozen and examined, but proved to be non-fluorescent. Acetone extracts of both normal and frozen potato tuber tissue were fluorescent, but similar extracts of both frozen and normal apple fruit were not. Although normal potato tissue is non-fluorescent and frozen tissue is brightly fluorescent, no differences were found in the brightness of the acetone extracts.

2001 ◽  
Vol 91 (6) ◽  
pp. 579-585 ◽  
Author(s):  
Aaron Levin ◽  
Alexander Baider ◽  
Evgenia Rubin ◽  
Ulrich Gisi ◽  
Yigal Cohen

The ability of Phytophthora infestans, the causal agent of potato and tomato late blight, to produce oospores in potato tuber tissue was studied in the field and under laboratory conditions. In 1998 and 2000 field experiments, the canopy of potato cvs. Alpha and Mondial, respectively, were coinoculated with A1 + A2 sporangia of the fungus, and the infected tubers collected at harvest were examined for the presence of oospores. In 1998, only 2 of 90 infected tubers had oospores, whereas none of the 90 tubers examined in 2000 had any oospores. In the latter experiment, infected tubers kept in storage up to 12 weeks after harvest had no oospores. Artificial co-inoculations of whole tubers with A1 + A2 sporangia resulted only rarely in the formation of oospores inside the tubers. Co-inoculations of potato tuber discs taken from dormant tubers 0 to 16 weeks after harvest failed to support any oospore production, whereas discs taken from sprouting tubers of ≥18 weeks after harvest allowed oospores to form. Tuber discs showed enhanced oospore formation when treated before inoculation with either sugars, amino acids, casein hydrolysate, β-sitosterol, or chloroethylphosphonic acid. In contrast, reducing airflow into the petri dishes where potato tuber discs were incubated reduced the number of oospores produced. The number of oospores produced in tuber tissue was lower compared with that in leaf tissue regardless of the origin of isolates used. The data show that the ability of Phytophthora infestans to produce oospores in potato tuber tissue is very limited and increases with tuber aging.


Plant Disease ◽  
1999 ◽  
Vol 83 (5) ◽  
pp. 469-473 ◽  
Author(s):  
Brendan A. Niemira ◽  
William W. Kirk ◽  
Jeffrey M. Stein

A method for quantification of late blight (Phytophthora infestans) in potato tuber tissue using a digital scanner and image analysis software is presented. The average reflective intensity of light reflected from the cut surface of sample tubers measures the darkened, diseased potato tuber tissue amid lighter, late blight-free tissue. In the absence of disease, potato variety, tuber shape, and tuber size do not influence the scan results. While digital quantification of late blight in tubers under controlled inoculation conditions is consistent, the digital assessments of late blight did not correspond exactly with those from a conventional subjective visual method. Used together, the methods can provide complementary information regarding varietal susceptibility to P. infestans development on the tuber surface and internal tuber tissue. The method of image analysis presented may be used to determine susceptibility of potato tubers to late blight in varietal development programs, storage research programs, or other tuber research programs.


1969 ◽  
Vol 46 (7) ◽  
pp. 227-233
Author(s):  
John F. Kelly ◽  
Howard L. Adams ◽  
Charles E. Cunningham

1995 ◽  
Vol 93 (2) ◽  
pp. 225-232 ◽  
Author(s):  
K. Oosterhaven ◽  
K. J. Hartmans ◽  
J. J. C. Scheffer ◽  
L. H. W. Plas

1987 ◽  
Vol 38 (7) ◽  
pp. 1219-1227 ◽  
Author(s):  
JOANNE C. CONOVER ◽  
JANET A. PRYKE

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