scholarly journals Genetically engineered flavonol enriched tomato fruit modulates chondrogenesis to increase bone length in growing animals

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Dharmendra Choudhary ◽  
Ashutosh Pandey ◽  
Sulekha Adhikary ◽  
Naseer Ahmad ◽  
Chitra Bhatia ◽  
...  
Keyword(s):  
Tomato Fruit ◽  
Genetically Engineered ◽  
Bone Length

scholarly journals Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B9 Content in Potato Tubers

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 734 ◽  
Author(s):  
Bruce R. Robinson ◽  
Carolina Garcia Salinas ◽  
Perla Ramos Parra ◽  
John Bamberg ◽  
Rocio I. Diaz de la Garza ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Tomato Fruit ◽  
Genetically Engineered ◽  
Food Crops ◽  
Great Success ◽  
Rice Grain ◽  
Rna Seq ◽  
Potato Tubers ◽  
Vitamin B9 ◽  
Folate Biosynthesis

Biofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and lack of economic model have prevented the widespread adoption of nutritionally-enhanced genetically-engineered (GE) food crops. Meanwhile, little effort has been made to biofortify food crops with folate by breeding. Previously we reported >10-fold variation in folate content in potato genotypes. To facilitate breeding for enhanced folate content, we attempted to identify genes that control folate content in potato tuber. For this, we analyzed the expression of folate biosynthesis and salvage genes in low- and high-folate potato genotypes. First, RNA-Seq analysis showed that, amongst all folate biosynthesis and salvage genes analyzed, only one gene, which encodes γ-glutamyl hydrolase 1 (GGH1), was consistently expressed at higher levels in high- compared to low-folate segregants of a Solanum boliviense Dunal accession. Second, quantitative PCR showed that GGH1 transcript levels were higher in high- compared to low-folate segregants for seven out of eight pairs of folate segregants analyzed. These results suggest that GGH1 gene expression is an indicator of folate content in potato tubers.

scholarly journals The Biochemistry and Control of Cell Disruption-dependent Aroma Generation by Tomatoes

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 554E-554
Author(s):  
E.A. Baldwin
Keyword(s):  
Volatile Compounds ◽  
Ethylene Production ◽  
Tomato Fruit ◽  
Genetically Engineered ◽  
Volatile Components ◽  
Flavor Components ◽  
Threshold Data ◽  
Odor Thresholds ◽  
Aroma Generation ◽  
And Control

More than 400 volatile components have been identified in tomato fruit, of which only 10–16 are likely to be important contributors to tomato flavor/aroma based on odor threshold data. Tomato volatiles are grouped as lipid-derived, carotenoid-related, amino acid-related, lignin-related, or of uncertain origin. These flavor components are either present in intact fruit or formed after blending due to mixing of previously compartmentalized enzymes and substrates. Lipid-derived volatiles are the biggest group containing cis-3-hexenal and hexanal, which are quantitatively the major volatile compounds in tomato fruit. cis-3-Hexenal and -ionone have the highest odor thresholds among tomato volatile compounds so far identified. Most of these compounds increase during ripening (or the enzymes, substrates and conditions develop that result in increased levels after blending) and appear to be related to ethylene production. Biosynthetic pathways have been established or suggested for most of the important flavor components, of which lipid degradation is the best-understood. Linoleic and linolenic acids are oxidized to hydroperoxides by lipoxygenase, which are then cleaved to volatile C6 aldehydes (hexanal and cis-3-hexenal, respectively). There are two membrane-associated lipoxygenases (tomloxA and B), of which tomloxB appears to be fruit-specific and increases during ripening. Alcohol dehydrogenase (ADH) has been demonstrated to catalyze the interconversion of trans-hexene-2-al and -2-ol and of trans-hexene-2-al, hexanal and hexanol. The enzyme product of the Adh2 gene is induced by 3% O2, and is developmentally expressed in fruit aside from anoxic induction. Naturally occurring mutants and genetically engineered tomatoes with reduced ethylene production, color and/or retarded ripening patterns show changes in volatile concentrations.

Function of the polygalacturonase convertor in ripening tomato fruit

1991 ◽  
Vol 82 (2) ◽  
pp. 237-242 ◽  
Author(s):  
Erik Knegt ◽  
Evert Vermeer ◽  
Caroline Pak ◽  
Johan Bruinsma
Keyword(s):  
Tomato Fruit

Reproductive health issues in patients with psoriasis (literature review)

2019 ◽  
Vol 1 (7) ◽  
pp. 5-8
Author(s):  
L. S. Kruglova ◽  
A. A. Osina ◽  
A. A. Khotko
Keyword(s):  
Drug Therapy ◽  
Reproductive Health ◽  
Literature Review ◽  
Pregnancy Outcomes ◽  
Adverse Pregnancy Outcome ◽  
Certolizumab Pegol ◽  
Genetically Engineered ◽  
Health Issues ◽  
Degree Of Confidence ◽  
Adverse Pregnancy

Among patients with psoriasis, approximately 50% are women and almost 75 % of them are under the age of 40 years. Thus, most women with psoriasis have childbearing potential. When pregnancy occurs in 22 % of patients, the activity of psoriasis persists, characteristic of the course before pregnancy, in 23 % of women, the course of the disease worsens. The article provides up-to-date data on the management of pregnant patients with psoriasis. To improve pregnancy outcomes in patients with psoriasis, it is important to prevent exacerbation of the disease. The choice of drug therapy in this case is based on an assessment of the ratio of the risk of undesirable effects of the drugs on the developing fetus and the risk of the development of exacerbation of psoriasis, which can cause an adverse pregnancy outcome. Despite the fact that the available clinical experience of using genetically engineered drugs is still limited, with a certain degree of confidence we can say that there is no increase in the risk of adverse pregnancy outcomes associated with therapy with certolizumab pegol.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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