scholarly journals Mucosal Tuft Cell Density Is Increased in Diarrhea-Predominant Irritable Bowel Syndrome Colonic Biopsies

2020 ◽  
Vol 11 ◽  
Author(s):  
Jessica Aigbologa ◽  
Maeve Connolly ◽  
Julliette M. Buckley ◽  
Dervla O'Malley
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Tuft Cell ◽  
Irritable Bowel ◽  
Colonic Biopsies

scholarly journals Excitation of Enteric Neurons by Supernatants of Colonic Biopsies From Irritable Bowel Syndrome Patients (IBS) is Linked to Visceral Sensitivity

2011 ◽  
Vol 140 (5) ◽  
pp. S-521 ◽  
Author(s):  
Sabine Buhner ◽  
Qin Li ◽  
Breg Braak ◽  
Tamira K. Klooker ◽  
Sheila Vignali ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Enteric Neurons ◽  
Visceral Sensitivity ◽  
Irritable Bowel ◽  
Colonic Biopsies

Sensitization of Peripheral Sensory Nerves by Mediators From Colonic Biopsies of Diarrhea-Predominant Irritable Bowel Syndrome Patients: A Role for PAR2

2013 ◽  
Vol 108 (10) ◽  
pp. 1634-1643 ◽  
Author(s):  
Eduardo E Valdez-Morales ◽  
Jeff Overington ◽  
Raquel Guerrero-Alba ◽  
Fernando Ochoa-Cortes ◽  
Charles O Ibeakanma ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Sensory Nerves ◽  
Irritable Bowel ◽  
Peripheral Sensory ◽  
Colonic Biopsies

scholarly journals Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Tarek Mazzawi ◽  
Doris Gundersen ◽  
Trygve Hausken ◽  
Magdy El-Salhy
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Large Intestine ◽  
Chromogranin A ◽  
Endocrine Cells ◽  
Common Marker ◽  
Before And After ◽  
A Cell ◽  
Colon And Rectum ◽  
Irritable Bowel

The large intestine contains five types of endocrine cells that regulate its functions by sensing its luminal contents and releasing specific hormones. Chromogranin A (CgA) is a common marker for the gastrointestinal endocrine cells, and it is abnormal in irritable bowel syndrome (IBS) patients. Most IBS patients relate their symptoms to certain food elements. The present study investigated the effect of dietary guidance on the total endocrine cells of the large intestine as detected by CgA in 13 IBS patients. Thirteen control subjects were also included. Each patient received three sessions of dietary guidance. Colonoscopies were performed on controls and patients (at baseline and at 3–9 months after receiving guidance). Biopsy samples from the colon and rectum were immunostained for CgA and quantified by computerized image analysis. The densities of CgA cells in the total colon (mean ± SEM) among the controls and the IBS patients before and after receiving dietary guidance were83.3±10.1,38.6±3.7, and64.7±4.2cells/mm2, respectively (P=0.0004), and were unchanged in the rectum. In conclusion, the increase in CgA cell density after receiving dietary guidance may reflect a change in the densities of the large intestinal endocrine cells causing an improvement in the IBS symptoms.

scholarly journals Chromogranin A cell density in the rectum of patients with irritable bowel syndrome

2012 ◽  
Vol 6 (6) ◽  
pp. 1223-1225 ◽  
Author(s):  
M. EL-SALHY ◽  
T. MAZZAWI ◽  
D. GUNDERSEN ◽  
T. HAUSKEN
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Chromogranin A ◽  
A Cell ◽  
Irritable Bowel

scholarly journals Increased gastric chromogranin A cell density following changes to diets of patients with irritable bowel syndrome

2014 ◽  
Vol 10 (5) ◽  
pp. 2322-2326 ◽  
Author(s):  
TAREK MAZZAWI ◽  
DORIS GUNDERSEN ◽  
TRYGVE HAUSKEN ◽  
MAGDY EL-SALHY
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Chromogranin A ◽  
A Cell ◽  
Irritable Bowel

scholarly journals Duodenal Chromogranin A Cell Density as a Biomarker for the Diagnosis of Irritable Bowel Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Magdy El-Salhy ◽  
Odd Helge Gilja ◽  
Doris Gundersen ◽  
Jan Gunnar Hatlebakk ◽  
Trygve Hausken
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Chromogranin A ◽  
Control Group ◽  
Common Marker ◽  
Sophisticated Equipment ◽  
A Cell ◽  
Gastrointestinal Complaints ◽  
Abc Method ◽  
Irritable Bowel

Background and Aim. Chromogranin A (CgA) is a common marker for endocrine cells. The density of duodenal CgA cells is reduced in patients with irritable bowel syndrome (IBS).Methods. The present study was undertaken to evaluate the density of duodenal CgA as a biomarker for the diagnosis of IBS. Two hundred and three patients with IBS were recruited (180 females and 23 males; mean age, 36 years; range, 18–66 years). The control group comprised 86 healthy subjects without gastrointestinal complaints (77 females and 9 males; mean age, 38 years; range, 18–67 years). Biopsy samples were taken from the duodenum during gastroscopy. Sections from these biopsy samples were immunostained for CgA using the avidin-biotin complex (ABC) method. CgA cell density was quantified by computerized image analysis.Results. The CgA cell density was lower in IBS-total and in all of the IBS subgroups than in the controls. The sensitivity and specificity for a cutoff of <200 cells/mm2were 86% and 95%, respectively.Conclusion. The duodenal CgA cell density seems to be a good biomarker for the diagnosis of IBS. It is an inexpensive, simple, and easy-to-use method that does not require sophisticated equipment or considerable experience.

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