scholarly journals Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Tarek Mazzawi ◽  
Doris Gundersen ◽  
Trygve Hausken ◽  
Magdy El-Salhy
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Large Intestine ◽  
Chromogranin A ◽  
Endocrine Cells ◽  
Common Marker ◽  
Before And After ◽  
A Cell ◽  
Colon And Rectum ◽  
Irritable Bowel

The large intestine contains five types of endocrine cells that regulate its functions by sensing its luminal contents and releasing specific hormones. Chromogranin A (CgA) is a common marker for the gastrointestinal endocrine cells, and it is abnormal in irritable bowel syndrome (IBS) patients. Most IBS patients relate their symptoms to certain food elements. The present study investigated the effect of dietary guidance on the total endocrine cells of the large intestine as detected by CgA in 13 IBS patients. Thirteen control subjects were also included. Each patient received three sessions of dietary guidance. Colonoscopies were performed on controls and patients (at baseline and at 3–9 months after receiving guidance). Biopsy samples from the colon and rectum were immunostained for CgA and quantified by computerized image analysis. The densities of CgA cells in the total colon (mean ± SEM) among the controls and the IBS patients before and after receiving dietary guidance were83.3±10.1,38.6±3.7, and64.7±4.2cells/mm2, respectively (P=0.0004), and were unchanged in the rectum. In conclusion, the increase in CgA cell density after receiving dietary guidance may reflect a change in the densities of the large intestinal endocrine cells causing an improvement in the IBS symptoms.

scholarly journals Duodenal Chromogranin A Cell Density as a Biomarker for the Diagnosis of Irritable Bowel Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Magdy El-Salhy ◽  
Odd Helge Gilja ◽  
Doris Gundersen ◽  
Jan Gunnar Hatlebakk ◽  
Trygve Hausken
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Chromogranin A ◽  
Control Group ◽  
Common Marker ◽  
Sophisticated Equipment ◽  
A Cell ◽  
Gastrointestinal Complaints ◽  
Abc Method ◽  
Irritable Bowel

Background and Aim. Chromogranin A (CgA) is a common marker for endocrine cells. The density of duodenal CgA cells is reduced in patients with irritable bowel syndrome (IBS).Methods. The present study was undertaken to evaluate the density of duodenal CgA as a biomarker for the diagnosis of IBS. Two hundred and three patients with IBS were recruited (180 females and 23 males; mean age, 36 years; range, 18–66 years). The control group comprised 86 healthy subjects without gastrointestinal complaints (77 females and 9 males; mean age, 38 years; range, 18–67 years). Biopsy samples were taken from the duodenum during gastroscopy. Sections from these biopsy samples were immunostained for CgA using the avidin-biotin complex (ABC) method. CgA cell density was quantified by computerized image analysis.Results. The CgA cell density was lower in IBS-total and in all of the IBS subgroups than in the controls. The sensitivity and specificity for a cutoff of <200 cells/mm2were 86% and 95%, respectively.Conclusion. The duodenal CgA cell density seems to be a good biomarker for the diagnosis of IBS. It is an inexpensive, simple, and easy-to-use method that does not require sophisticated equipment or considerable experience.

scholarly journals Chromogranin A cell density in the large intestine of Asian and European patients with irritable bowel syndrome

2017 ◽  
Vol 52 (6-7) ◽  
pp. 691-697 ◽  
Author(s):  
Magdy El-Salhy ◽  
Tanisa Patcharatrakul ◽  
Jan Gunnar Hatlebakk ◽  
Trygve Hausken ◽  
Odd Helge Gilja ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Large Intestine ◽  
Chromogranin A ◽  
A Cell ◽  
Irritable Bowel

Difference of Chromogranin a Cell Density in the Large Intestine Between Asian and European Patients with Irritable Bowel Syndrome (IBS)

2017 ◽  
Vol 152 (5) ◽  
pp. S728
Author(s):  
Tanisa Patcharatrakul ◽  
Magdy El-Salhy ◽  
Jan Gunnar Hatlebakk ◽  
Trygve Hausken ◽  
Odd Helge Gilja ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Large Intestine ◽  
Chromogranin A ◽  
A Cell ◽  
Irritable Bowel

scholarly journals Chromogranin A cell density in the rectum of patients with irritable bowel syndrome

2012 ◽  
Vol 6 (6) ◽  
pp. 1223-1225 ◽  
Author(s):  
M. EL-SALHY ◽  
T. MAZZAWI ◽  
D. GUNDERSEN ◽  
T. HAUSKEN
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Chromogranin A ◽  
A Cell ◽  
Irritable Bowel

scholarly journals Increased gastric chromogranin A cell density following changes to diets of patients with irritable bowel syndrome

2014 ◽  
Vol 10 (5) ◽  
pp. 2322-2326 ◽  
Author(s):  
TAREK MAZZAWI ◽  
DORIS GUNDERSEN ◽  
TRYGVE HAUSKEN ◽  
MAGDY EL-SALHY
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Chromogranin A ◽  
A Cell ◽  
Irritable Bowel

scholarly journals Reduced chromogranin A cell density in the ileum of patients with irritable bowel syndrome

2013 ◽  
Vol 7 (4) ◽  
pp. 1241-1244 ◽  
Author(s):  
MAGDY EL-SALHY ◽  
INGVILD HAUKAAS WENDELBO ◽  
DORIS GUNDERSEN
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cell Density ◽  
Chromogranin A ◽  
A Cell ◽  
Irritable Bowel

Bifidobacterium infantis M-63 improves mental health in victims with irritable bowel syndrome developed after a major flood disaster

2019 ◽  
Vol 10 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Z.F. Ma ◽  
N. Yusof ◽  
N. Hamid ◽  
R.M. Lawenko ◽  
W.M.Z. Wan Mohammad ◽  
...  
Keyword(s):  
Mental Health ◽  
Irritable Bowel Syndrome ◽  
Bodily Pain ◽  
Depression Scale ◽  
Bacterial Overgrowth ◽  
Well Being ◽  
Bifidobacterium Infantis ◽  
Before And After ◽  
Irritable Bowel ◽  
Lower Ratio

Individuals in a community who developed irritable bowel syndrome (IBS) after major floods have significant mental health impairment. We aimed to determine if Bifidobacterium infantis M-63 was effective in improving symptoms, psychology and quality of life measures in flood-affected individuals with IBS and if the improvement was mediated by gut microbiota changes. Design was non-randomised, open-label, controlled before-and-after. Of 53 participants, 20 with IBS were given B. infantis M-63 (1×109 cfu/sachet/day) for three months and 33 were controls. IBS symptom severity scale, hospital anxiety and depression scale, SF-36 Questionnaire, hydrogen breath testing for small intestinal bacterial overgrowth and stools for 16S rRNA metagenomic analysis were performed before and after intervention. 11 of 20 who were given probiotics (M-63) and 20 of 33 controls completed study as per-protocol. Mental well-being was improved with M-63 vs controls for full analysis (P=0.03) and per-protocol (P=0.01) populations. Within-group differences were observed for anxiety and bodily pain (both P=0.04) in the M-63 per-protocol population. Lower ratio of Firmicutes/Bacteroidetes was observed with M-63 vs controls (P=0.01) and the lower ratio was correlated with higher post-intervention mental score (P=0.04). B. infantis M-63 is probably effective in improving mental health of victims who developed IBS after floods and this is maybe due to restoration of microbial balance and the gut-brain axis. However, our conclusion must be interpreted within the context of limited sample size. The study was retrospectively registered on 12 October 2017 and the Trial Registration Number (TRN) was NCT03318614.

Effect of sex on perception of rectosigmoid stimuli in irritable bowel syndrome

2006 ◽  
Vol 291 (2) ◽  
pp. R277-R284 ◽  
Author(s):  
Lin Chang ◽  
Emeran A. Mayer ◽  
Jennifer S. Labus ◽  
Max Schmulson ◽  
Oh Young Lee ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Sigmoid Colon ◽  
Healthy Controls ◽  
Perceptual Sensitivity ◽  
Control Groups ◽  
Healthy Men ◽  
Before And After ◽  
Irritable Bowel ◽  
And Control ◽  
The Relationship

In irritable bowel syndrome (IBS) patients, the relationship between sex and sensitivity to visceral stimuli is incompletely understood. Our aim was to evaluate the effect of sex on perceptual responses to visceral stimulation in IBS. Fifty-eight IBS patients (mean age 42 ± 1 yr; 34 men, 24 women) and 26 healthy controls (mean age 38 ± 3 yr; 9 men, 17 women) underwent barostat-assisted distensions of the rectum and sigmoid colon. Rectal discomfort thresholds were measured using a randomized, phasic distension paradigm before and after repeated noxious sigmoid stimulation (SIG, 60-mmHg pulses). Sex had a significant effect on rectal discomfort thresholds. Women with IBS were the most sensitive (lower thresholds [27 ± 2.7 mmHg] and higher ratings), with significantly lower rectal discomfort thresholds compared with men with IBS (38 ± 2.3 mmHg) and healthy women who were the least sensitive (41.9 ± 3.2 mmHg; both P < 0.01). There were no significant differences in rectal discomfort thresholds between healthy men (34 ± 4.3 mmHg) and men with IBS. Across both IBS and control groups, women demonstrated a significant lowering of discomfort thresholds after noxious sigmoid stimulation ( P < 0.01), while men did not. Sex significantly influences perceptual sensitivity to rectosigmoid distension. Women show greater perceptual responses to this paradigm.

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