scholarly journals Live Tissue Imaging Sheds Light on Cell Level Events During Ectodermal Organ Development

2020 ◽  
Vol 11 ◽  
Author(s):  
Isabel Mogollón ◽  
Laura Ahtiainen
Keyword(s):  
Tissue Imaging ◽  
Organ Development ◽  
Cell Level ◽  
Live Tissue

The frontier of live tissue imaging across space and time

2021 ◽  
Vol 28 (4) ◽  
pp. 603-622
Author(s):  
Qiang Huang ◽  
Aliesha Garrett ◽  
Shree Bose ◽  
Stephanie Blocker ◽  
Anne C. Rios ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Live Tissue ◽  
Space And Time

scholarly journals Live Tissue Imaging Shows Reef Corals Elevate pH under Their Calcifying Tissue Relative to Seawater

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e20013 ◽  
Author(s):  
Alexander Venn ◽  
Eric Tambutté ◽  
Michael Holcomb ◽  
Denis Allemand ◽  
Sylvie Tambutté
Keyword(s):  
Tissue Imaging ◽  
Reef Corals ◽  
Live Tissue

Live Tissue Imaging with Conventional Confocal Microscopy

2007 ◽  
pp. 79-85
Author(s):  
Marshall H. Montrose ◽  
Alastair J. M. Watson
Keyword(s):  
Confocal Microscopy ◽  
Tissue Imaging ◽  
Live Tissue

scholarly journals Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy

2011 ◽  
Vol 16 (11) ◽  
pp. 116009 ◽  
Author(s):  
Jiun-Yann Yu ◽  
Chun-Hung Kuo ◽  
Daniel B. Holland ◽  
Yenyu Chen ◽  
Mingxing Ouyang ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Tissue Imaging ◽  
Wide Field ◽  
Optical Sectioning ◽  
Live Tissue ◽  
Plane Projection

scholarly journals Unbiased automated quantitation of ROS signals in live retinal neurons of Drosophila using Fiji/ImageJ

BioTechniques ◽  
2021 ◽  
Author(s):  
Prajakta Deshpande ◽  
Neha Gogia ◽  
Anuradha Venkatakrishnan Chimata ◽  
Amit Singh
Keyword(s):  
Signal To Noise Ratio ◽  
Tissue Imaging ◽  
Oxygen Species ◽  
Proof Of Concept ◽  
Retinal Neurons ◽  
Eye Model ◽  
Cellular Processes ◽  
Live Tissue ◽  
Model Of Alzheimer’S Disease ◽  
Quantitation Method

Numerous imaging modules are utilized to study changes that occur during cellular processes. Besides qualitative (immunohistochemical) or semiquantitative (Western blot) approaches, direct quantitation method(s) for detecting and analyzing signal intensities for disease(s) biomarkers are lacking. Thus, there is a need to develop method(s) to quantitate specific signals and eliminate noise during live tissue imaging. An increase in reactive oxygen species (ROS) such as superoxide (O2•-) radicals results in oxidative damage of biomolecules, which leads to oxidative stress. This can be detected by dihydroethidium staining in live tissue(s), which does not rely on fixation and helps prevent stress on tissues. However, the signal-to-noise ratio is reduced in live tissue staining. We employ the Drosophila eye model of Alzheimer's disease as a proof of concept to quantitate ROS in live tissue by adapting an unbiased method. The method presented here has a potential application for other live tissue fluorescent images.

scholarly journals Live Tissue Imaging to Elucidate Mechanical Modulation of Stem Cell Niche Quiescence

2016 ◽  
Vol 6 (1) ◽  
pp. 285-292 ◽  
Author(s):  
Nicole Y.C. Yu ◽  
Connor A. O'Brien ◽  
Iveta Slapetova ◽  
Renee M. Whan ◽  
Melissa L. Knothe Tate
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Tissue Imaging ◽  
Live Tissue ◽  
Mechanical Modulation ◽  
Cell Niche
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