scholarly journals Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy

2011 ◽  
Vol 16 (11) ◽  
pp. 116009 ◽  
Author(s):  
Jiun-Yann Yu ◽  
Chun-Hung Kuo ◽  
Daniel B. Holland ◽  
Yenyu Chen ◽  
Mingxing Ouyang ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Tissue Imaging ◽  
Wide Field ◽  
Optical Sectioning ◽  
Live Tissue ◽  
Plane Projection

scholarly journals Overcoming tissue scattering in wide-field deep imaging by extended detection and computational reconstruction

2019 ◽  
Author(s):  
Yuanlong Zhang ◽  
Tiankuang Zhou ◽  
Xuemei Hu ◽  
Hao Xie ◽  
Lu Fang ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Tissue Imaging ◽  
Wide Field ◽  
Temporal Focusing ◽  
Tissue Scattering ◽  
Deep Imaging ◽  
Detection Mode ◽  
Computational Reconstruction ◽  
Imaging Depth

AbstractCompared to the golden technique of point‐scanning multiphoton microscopy, line‐scanning temporal focusing microscopy (LTFM) is competitive in high imaging speed while maintaining tight axial confinement. However, considering its wide‐field detection mode, LTFM suffers from shallow penetration depth as a result of crosstalk induced by tissue scattering. In contrast to the spatial filtering based on confocal slit detection, we propose the extended detection LTFM (ED‐LTFM), the first technique to extract signals from scattered photons and thus effectively extend the imaging depth. By recording a succession of line‐shape excited signals in 2D and reconstructing signals under Hessian regularization, we can push the depth limitation in scattering tissue imaging. We valid the concept with numerical simulations, and demonstrate the performance of enhanced imaging depth in in vivo imaging of mouse brains.

Signal to Noise Considerations in Single Pixel Multiphoton Microscopy

2020 ◽  
Vol 5 (5) ◽  
pp. 1-2
Author(s):  
Dushan Wadduwage
Keyword(s):  
Multiphoton Microscopy ◽  
Tissue Imaging ◽  
Wide Field ◽  
Signal To Noise ◽  
Deep Tissue ◽  
Multiplexed Imaging ◽  
Deep Tissue Imaging ◽  
Single Pixel ◽  
Noise Metrics

Single-pixel imaging geometries for wide-field multiphoton microscopy (SPx-MPM) have emerged as a contender to conventional point-scanning multiphoton systems (PS-MPM) for deep tissue imaging. These systems are thought to be faster due to their multiplexed imaging capabilities with higher photon throughput. In this study we numerically compare the signal to noise metrics of the SPx-MPM to the PS-MPM systems. Our results suggest that PS-MPM systems outperform SPx-MPM systems, despite their higher photon throughput.

scholarly journals Challenges in 3D Live Cell Imaging

Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 275
Author(s):  
Herbert Schneckenburger ◽  
Verena Richter
Keyword(s):  
Live Cell Imaging ◽  
Laser Scanning ◽  
Cell Imaging ◽  
Live Cell ◽  
Laser Scanning Microscopy ◽  
Radiative Energy ◽  
Wide Field ◽  
Optical Sectioning ◽  
Continuous Increase ◽  
Radiative Energy Transfer

A short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. Furthermore, enhanced methods of wide-field or laser scanning microscopy together with some relevant examples and applications are summarized. In the future one may profit from a continuous increase in microscopic resolution, but also from molecular sensing techniques in the nanometer range using e.g., non-radiative energy transfer (FRET).

scholarly journals Light sheet-excited spontaneous Raman imaging of a living fish by optical sectioning in a wide field Raman microscope

2012 ◽  
Vol 20 (15) ◽  
pp. 16195 ◽  
Author(s):  
Yusuke Oshima ◽  
Hidetoshi Sato ◽  
Hiroko Kajiura-Kobayashi ◽  
Tetsuaki Kimura ◽  
Kiyoshi Naruse ◽  
...  
Keyword(s):  
Light Sheet ◽  
Raman Imaging ◽  
Wide Field ◽  
Optical Sectioning

scholarly journals Multiphoton Microscopy for Ophthalmic Imaging

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Emily A. Gibson ◽  
Omid Masihzadeh ◽  
Tim C. Lei ◽  
David A. Ammar ◽  
Malik Y. Kahook
Keyword(s):  
Harmonic Generation ◽  
Second Harmonic ◽  
Imaging Techniques ◽  
Multiphoton Microscopy ◽  
Tissue Imaging ◽  
Third Harmonic ◽  
Two Photon ◽  
Disease Mechanisms ◽  
Ophthalmic Imaging ◽  
Anti Stokes

We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functional tissue imaging. MPM is compared with current clinical ophthalmological imaging techniques such as reflectance confocal microscopy, optical coherence tomography, and fluorescence imaging. In addition, we discuss the future prospects for MPM in disease detection and clinical monitoring of disease progression, understanding fundamental disease mechanisms, and real-time monitoring of drug delivery.

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