scholarly journals Proceeding From in vivo Functions of Pheromone Receptors: Peripheral-Coding Perception of Pheromones From Three Closely Related Species, Helicoverpa armigera, H. assulta, and Heliothis virescens

2018 ◽  
Vol 9 ◽  
Author(s):  
Bing Wang ◽  
Yang Liu ◽  
Gui-Rong Wang
Keyword(s):  
Helicoverpa Armigera ◽  
Related Species ◽  
Heliothis Virescens ◽  
Closely Related Species ◽  
Helicoverpa Armígera

Potential detoxification of gossypol by UDP-glycosyltransferases in the two Heliothine moth species Helicoverpa armigera and Heliothis virescens

2016 ◽  
Vol 71 ◽  
pp. 49-57 ◽  
Author(s):  
Corinna Krempl ◽  
Theresa Sporer ◽  
Michael Reichelt ◽  
Seung-Joon Ahn ◽  
Hanna Heidel-Fischer ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Heliothis Virescens ◽  
Helicoverpa Armígera ◽  
Moth Species

scholarly journals Potensi Nematoda Patogen Serangga Steinernema spp. dalam Pengendalian Hama Utama Tanaman Kapas

2016 ◽  
Vol 1 (2) ◽  
pp. 101
Author(s):  
Heri Prabowo ◽  
I.G.A.A. Indrayani
Keyword(s):  
Helicoverpa Armigera ◽  
Insect Pests ◽  
Soil Surface ◽  
Tenebrio Molitor ◽  
Pectinophora Gossypiella ◽  
Helicoverpa Armígera ◽  
Soy Bean Oil

<p>Steinernema spp. memiliki potensi untuk mengendalikan hama tanaman kapas seperti Helicoverpa armigera dan Pectinophora gossypiella. Steinernema spp. mampu menyebabkan mortalitas P. gossypiella dan H. armi-gera berturut-turut sebesar 31,6–55,4 dan 46,3–63,8%. Steinernema spp. memiliki kemampuan membunuh lebih baik pada P. gossypiella, sedangkan kemampuan reproduksi dalam inangnya lebih baik pada H. armi-gera. Steinernema spp. mampu menginfeksi serangga inang lebih baik pada stadium ulat lebih tua diban-dingkan stadium muda. Steinernema spp. dapat diproduksi secara in vivo dan in vitro. Produksi secara in vivo dapat menggunakan Tenebrio molitor, Tirathaba rufivena, dan Attacus atlas. Produksi secara in vitro dapat menggunakan usus ayam, lemak sapi, dan minyak kedelai. Perlu dikembangkan formulasi Steinerne-ma spp. yang murah dan efektif untuk mengendalikan hama di atas permukaan tanah. Selain itu diperlukan pencarian isolat Steinernema spp. yang virulen dan cepat membunuh hama sasaran.</p><p> </p><p>Steinernema spp. could be potentially used for controlling H. armigera and P. gossypiella on cotton. Steiner-nema spp. causes mortality on P. gossypiella and H. armigera 31,6–55,4 and 46,3–63,8% respectively. The nematode causes a higher mortality on P. gossypiella than on H. armigera, however, produces more juvenile infective on H. armigera than on P. gossypiella. Higher successful infections of Steinernema spp. occurs on late larval stadium than on early one. Production of Steinernema spp. can be in vivo using Tenebrio molitor, Tirathaba rufivena, and Attacus atlas; and in vitro using chicken intestinum, cow lipid, and soy bean oil. For effecttively use, this nematode need to be formulated especially for controlling insect pests on soil surface, as well as finding the more virulent isolates against the target insects.</p>

scholarly journals Diminution of feeding response in Helicoverpa armigera by inhibition or silencing of sugar gustatory receptor

2019 ◽  
Author(s):  
Aniruddha R. Agnihotri ◽  
Sanyami S. Zunjarrao ◽  
Mukta Nagare ◽  
Rakesh S. Joshi
Keyword(s):  
Helicoverpa Armigera ◽  
Feeding Rate ◽  
Receptor Activation ◽  
Inhibitory Effect ◽  
Gustatory Receptor ◽  
Feeding Response ◽  
Downstream Signaling ◽  
Helicoverpa Armígera ◽  
Novel Strategy

ABSTRACTGustatory receptor (GR) is one of the essential chemosensory molecules in Lepidopteran pests. GR is involved in sensing several canonical tastes which in turn regulate the diverse behavioral and physiological responses of these insects. In this article, we have evaluated the alteration in feeding response of Helicoverpa armigera by blocking and silencing of sugar-sensing gustatory receptor 9 (HarmGr9). Sf9 cells based assay showed that glucose analogue, Miglitol, can bind to the expressed HarmGr9. This binding might lead to an inhibition of receptor activation and downstream signaling, indicated by reduced intracellular Ca2+ fluorescence. Further, the in-vivo study illustrated the feeding rate reduced on a diet containing miglitol as compared to the larvae fed on the artificial diet. Reduction in feeding rate was prolonged in insects fed on the miglitol containing diet even after switching to the control glucose diet. Competitive cell-based assay and feeding assay, using equimolar glucose and miglitol, also showed an inhibitory effect on HarmGr-9 activation and insect feeding rate. We have observed similar feeding rate reduction in HarmGr9 knockdown in H. armigera larvae. We believe this unique approach of H. armigera feeding response inhibition by blocking the sugar receptor can be further used to develop a novel strategy for agricultural pest management.

In Silico and in Vivo Investigations of Bioagent Helicoverpa Nucleopolyhedrovirus against Helicoverpa armigera in Chickpea

2021 ◽  
Vol 9 (2) ◽  
pp. 21-32
Author(s):  
Ritu Srivastava ◽  
◽  
Amritesh Chandra Shukla ◽  
Keyword(s):  
Green Tea ◽  
Rice Bran ◽  
Helicoverpa Armigera ◽  
Maximum Yield ◽  
3D Structure ◽  
Homology Model ◽  
In Vitro Toxicity ◽  
Helicoverpa Armígera

During investigations; homology model of 3D-structure was built for sequence of polyhedrin protein of Helicoverpa armigera nucleopolyhedrovirus, containing 246 amino acids (Accession: ACI05106.1 GI: 205946055), and evaluated through multiple tools/ applications to judge extent of accuracy in light of existing crystal structure. Further, in vivo experiments were conducted and determined response of different adjuvants with HaNPV and their efficacy. The pooled mean mortality of larvae exposed to virus mixed with 5% green tea and 5% rice bran filtrates (8.3 larvae per 25 plants) was differ significantly from control (15.8 larvae per 25 plants), suggesting that UV protectants & diet enhancer (mannitol) has ability to protect stability of virulence of the virus, under field conditions. The minimum percent pod damage of 8.6% and maximum yield of 1604.8 Kg ha-1 at harvesting was recorded with formulation of indigenous BHA virus isolate @ 2.2 x 105 POBs mL-1 mixed with Roket @50 ppm; followed by formulation with mannitol (@ 1% + green tea 5% + 5% rice bran filtrates) with percent pod damage of 16.8 % and yield of 1045.8 Kg ha-1 of chickpea. Furthermore, in vitro toxicity of fresh virus suspension @ 250 mL ha-1 was recorded more toxic in terms of percent mortality and LT50 (5.65 days). However, three months stored HaNPV formulations [(A) mannitol @ 1%+ green tea@ 5% and (B) mannitol @ 1% + green tea 5% + 5% rice bran filtrates] were more effective in larval reduction with LT50 of 7.89 and 6.00 days, respectively. Virus mixed with 5% green tea and 5% rice bran filtrates gave stability to formulation up-to one year with LT50 of 7.64 days. Findings showed that HaNPV formulations with mannitol (B) have potential that can be used in integrated manner with other IPM practices, to reduce the use of toxic synthetic pesticides in chickpea.

Effects of dietary quercetin on performance and cytochrome P450 expression of the cotton bollworm, Helicoverpa armigera

2015 ◽  
Vol 105 (6) ◽  
pp. 771-777 ◽  
Author(s):  
D. Liu ◽  
Y. Yuan ◽  
M. Li ◽  
X. Qiu
Keyword(s):  
Cytochrome P450 ◽  
Helicoverpa Armigera ◽  
Adult Emergence ◽  
Cotton Bollworm ◽  
Molecular Response ◽  
Terrestrial Plants ◽  
P450 Expression ◽  
Helicoverpa Armígera

AbstractQuercetin is ubiquitous in terrestrial plants. The cotton bollworm Helicoverpa armigera as a highly polyphagous insect has caused severe crop losses. Until now, interactions between this pest and quercetin are poorly understood at the biochemical and molecular levels. In this study, we investigated the in vivo effects of quercetin on performance of cotton bollworm and on cytochrome P450 (P450) expression. Deleterious effects of quercetin on the performance of the cotton bollworm, including growth, survival, pupation and adult emergence were observed after oral administration of 3 and 10 mg g−1 quercetin to larvae since the third instar, whereas no significant toxic effect was found at 0.1 mg g−1 quercetin treatment. Piperonyl butoxide treatment enhanced the toxicity of quercetin. In vitro metabolism studies showed that quercetin was rapidly transformed by gut enzymes of fifth instar larvae of the cotton bollworm. qRT–PCR results revealed that the effect of quercetin on P450 expression was tissue- and dose-specific. Quercetin regulated P450 expression in a mild manner, and it could serve as P450 inducer (CYP337B1, CYP6B6) or repressor (CYP337B1, CYP6B7, CYP6B27, CYP9A14, CYP6AE11, and CYP4M7). These findings are important for advancing our understanding of the biochemical and molecular response of insects to plant toxins and have implications for a smart pest control.

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