A Real‐time PCR assay for rapid identification of Helicoverpa armigera (Lepidoptera: Noctuidae) from closely related species

2021 ◽  
Author(s):  
Asha Thomas ◽  
Dongmei Li ◽  
Disna Gunawardana ◽  
Alan Flynn
Keyword(s):  
Real Time ◽  
Helicoverpa Armigera ◽  
Real Time Pcr ◽  
Related Species ◽  
Rapid Identification ◽  
Pcr Assay ◽  
Closely Related Species ◽  
Helicoverpa Armígera ◽  
Lepidoptera Noctuidae

scholarly journals A Multiplex Real-Time PCR Assay to Diagnose and Separate Helicoverpa armigera and H. zea (Lepidoptera: Noctuidae) in the New World

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142912 ◽  
Author(s):  
Todd M. Gilligan ◽  
Luke R. Tembrock ◽  
Roxanne E. Farris ◽  
Norman B. Barr ◽  
Marja J. van der Straten ◽  
...  
Keyword(s):  
Real Time ◽  
Helicoverpa Armigera ◽  
Real Time Pcr ◽  
New World ◽  
Pcr Assay ◽  
Helicoverpa Armígera ◽  
Lepidoptera Noctuidae

scholarly journals A TaqMan Probe-Based Real-Time PCR Assay for the Rapid Identification of the Emerging Multidrug-Resistant Pathogen Candida auris on the BD Max System

2019 ◽  
Vol 57 (7) ◽  
Author(s):  
Amorce Lima ◽  
Raymond Widen ◽  
Grant Vestal ◽  
Dominic Uy ◽  
Suzane Silbert
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Multidrug Resistant ◽  
Rapid Identification ◽  
Taqman Probe ◽  
Pcr Assay ◽  
Candida Auris ◽  
Closely Related Species ◽  
Content Type ◽  
Deep Wound

ABSTRACT Candida auris is an emerging multidrug-resistant fungal pathogen that has been associated with nosocomial bloodstream and deep wound infections causing a high mortality rate mainly in intensive care unit (ICU) patients. Laboratories currently rely on phenotypic testing using commercial automated systems for identification of yeasts; however, this technique has often led to misidentification of C. auris to other closely related species. We developed and validated a TaqMan-based real-time PCR assay on the BD Max platform targeting ribosomal DNA (rDNA) region nucleotide sequences to quickly and accurately test for C. auris infection from culture and clinical specimens. The assay is highly specific, reproducible, and sensitive, allowing detection of as low as 1 C. auris CFU per reaction within 3 h.

scholarly journals Rapid identification of Bactrocera zonata (Dip.: Tephritidae) using TaqMan real-time PCR assay

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0205136 ◽  
Author(s):  
Marzieh Koohkanzade ◽  
Mohammad Zakiaghl ◽  
Manpreet K. Dhami ◽  
Lida Fekrat ◽  
Hussein Sadeghi Namaghi
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Identification ◽  
Pcr Assay ◽  
Bactrocera Zonata

A Multi-Target Real-Time PCR Assay for Rapid Identification of Meningitis-Associated Microorganisms

2012 ◽  
Vol 53 (1) ◽  
pp. 74-79 ◽  
Author(s):  
Marco Favaro ◽  
Vincenzo Savini ◽  
Cartesio Favalli ◽  
Carla Fontana
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Identification ◽  
Pcr Assay
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