scholarly journals Growth and Development of Helicoverpa armigera (Lepidoptera: Noctuidae) Larvae Infected by Heliothis virescens ascovirus 3i (HvAV-3i)

2020 ◽  
Vol 11 ◽  
Author(s):  
Gong Chen ◽  
Hang Liu ◽  
Bo-Cheng Mo ◽  
Jue Hu ◽  
Shuang-Qing Liu ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Heliothis Virescens ◽  
Growth And Development ◽  
Helicoverpa Armígera ◽  
Heliothis Virescens Ascovirus ◽  
Lepidoptera Noctuidae

scholarly journals Characterization of Heliothis Virescens Ascovirus 3h orf21 that Encodes a Virion Protein

2020 ◽  
Vol 8 (4) ◽  
pp. 59-70
Author(s):  
Ying Zhao ◽  
Huan Yu ◽  
Ni Li ◽  
GuoHua Huang
Keyword(s):  
Helicoverpa Armigera ◽  
Heliothis Virescens ◽  
Polyclonal Antiserum ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Virion Protein ◽  
Helicoverpa Armígera ◽  
Single Transcript ◽  
Heliothis Virescens Ascovirus

Homologues of Heliothis virescens ascovirus 3h (HvAV-3h) orf21 are found in 9 completely sequenced members of the ascoviruses, but so far their functions are unknown. Here, orf21 (3h-21) was cloned in-frame into a pET-28a bacterial expression vector. The fusion protein produced by this construct was used for the preparation of a polyclonal antiserum. RT-PCR analysis showed a single transcript of 3h-21 of approximately 0.7kb was transcribed beginning at 24h post-infection in infected Helicoverpa armigera larvae. Western blot analysis of extracts from HvAv-3h-infected Helicoverpa armigera larvae detected a 25.6 kDa protein late in infection. This antiserum also reacted with a 25.6 kDa protein in purified virions of HvAv-3h. The protein was not extensively modified post-translation. Immunoelectron microscopy confirmed that the 3H-21 is associated with the structure of HvAV-3h virions.

Incidence of Helicoverpa armigera (Lepidoptera: Noctuidae) and its natural enemies on smallholder crops in Kenya

1993 ◽  
Vol 83 (3) ◽  
pp. 321-328 ◽  
Author(s):  
H. van den Berg ◽  
M. J. W. Cock ◽  
G. I. Oduor ◽  
E. K. Onsongo
Keyword(s):  
Helicoverpa Armigera ◽  
Heliothis Armigera ◽  
Population Densities ◽  
Parasitoid Species ◽  
Larval Parasitoid ◽  
Total Percentage ◽  
Four Seasons ◽  
Helicoverpa Armígera ◽  
Lepidoptera Noctuidae ◽  
Experimental Plots

AbstractSmallholder crops (sunflower, maize, sorghum and cotton) were grown in experimental plots at seven sites, representing different agricultural zones of Kenya, over four seasons. Helicoverpa armigera (Hübner) (formerly Heliothis armigera) only occasionally achieved population densities sufficient to cause obvious damage to the crops, and was virtually absent from the coastal sites. At the inland sites, infestation and mortality levels varied greatly. Information is presented on the incidence of H. armigera, and the identity, distribution and frequency of its common parasitoids and (potential) predators, sampled in the experimental plots. Trichogrammatoidea spp., egg parasitoids, and Linnaemya longirostris (Macquart), a tachinid late-larval parasitoid, were the most common parasitoid species, but total percentage parasitism was rather low. Of the large complex of predators, only anthocorids and ants (predominantly Pheidole spp., Myrmicaria spp. and Camponotus spp.) were sufficiently common and widespread to be of importance in suppressing H. armigera. The abundance of predators fluctuated widely between sites, but anthocorids were most abundant at the western sites.

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