scholarly journals Modulation of Retrograde Trafficking of KCa3.1 in a Polarized Epithelium

2017 ◽  
Vol 8 ◽  
Author(s):  
Bob Shih-Liang Lee ◽  
Daniel C. Devor ◽  
Kirk L. Hamilton
Keyword(s):  
Retrograde Trafficking ◽  
Polarized Epithelium

scholarly journals An EGFR autocrine loop encodes a slow‐reacting but dominant mode of mechanotransduction in a polarized epithelium

2010 ◽  
Vol 24 (5) ◽  
pp. 1604-1615 ◽  
Author(s):  
Nikola Kojic ◽  
Euiheon Chung ◽  
Alvin T. Kho ◽  
Jin‐Ah Park ◽  
Austin Huang ◽  
...  
Keyword(s):  
Dominant Mode ◽  
Autocrine Loop ◽  
Polarized Epithelium

scholarly journals Ride the wave: Retrograde trafficking becomes Ca2+ dependent with BAIAP3

2017 ◽  
Vol 216 (7) ◽  
pp. 1887-1889 ◽  
Author(s):  
Jakob B. Sørensen
Keyword(s):  
Angiogenesis Inhibitor ◽  
Secretory Vesicle ◽  
Vesicle Formation ◽  
Retrograde Trafficking ◽  
Trans Golgi Network ◽  
Cell Biol ◽  
Golgi Network

The functions of four of the five proteins in the mammalian uncoordinated-13 (Munc13) family have been identified as priming factors in SNARE-dependent exocytosis. In this issue, Zhang et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201702099) show that the fifth member, BAIAP3 (brain-specific angiogenesis inhibitor I–associated protein 3), acts in retrograde trafficking by returning secretory vesicle material to the trans-Golgi network. In its absence, secretory vesicle formation is impaired, leading to accumulation of immature vesicles, or lysosomal vesicle degradation.

scholarly journals Pseudomonas aeruginosa-Mediated Damage Requires Distinct Receptors at the Apical and Basolateral Surfaces of the Polarized Epithelium

2009 ◽  
Vol 78 (3) ◽  
pp. 939-953 ◽  
Author(s):  
Iwona Bucior ◽  
Keith Mostov ◽  
Joanne N. Engel
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mdck Cells ◽  
Three Dimensional ◽  
Opportunistic Pathogen ◽  
Necessary And Sufficient ◽  
Plastic Surfaces ◽  
Pharmacologic Inhibition ◽  
Increased Susceptibility ◽  
Polarized Epithelium

ABSTRACT Pseudomonas aeruginosa, an important opportunistic pathogen of humans, exploits epithelial damage to establish infection. We have rigorously explored the role of N-glycoproteins and heparan sulfate proteoglycans (HSPGs) in P. aeruginosa-mediated attachment and subsequent downstream events at the apical (AP) and basolateral (BL) surfaces of polarized epithelium. We demonstrate that the N-glycan chains at the AP surface are necessary and sufficient for binding, invasion, and cytotoxicity to kidney (MDCK) and airway (Calu-3) cells grown at various states of polarization on Transwell filters. Upregulation of N-glycosylation enhanced binding, whereas pharmacologic inhibition of N-glycosylation or infection of MDCK cells defective in N-glycosylation resulted in decreased binding. In contrast, at the BL surface, the HS moiety of HSPGs mediated P. aeruginosa binding, cytotoxicity, and invasion. In incompletely polarized epithelium, HSPG abundance was increased at the AP surface, explaining its increased susceptibility to P. aeruginosa colonization and damage. Using MDCK cells grown as three-dimensional cysts as a model for epithelial organs, we show that P. aeruginosa specifically colocalized with HS-rich areas at the BL membrane but with complex N-glycans at the AP surface. Finally, P. aeruginosa bound to HS chains and N-glycans coated on plastic surfaces, showing the highest binding affinity toward isolated HS chains. Together, these findings demonstrate that P. aeruginosa recognizes distinct receptors on the AP and BL surfaces of polarized epithelium. Changes in the composition of N-glycan chains and/or in the distribution of HSPGs may explain the enhanced susceptibility of damaged epithelium to P. aeruginosa.

scholarly journals Salmonella Inhibits Retrograde Trafficking of Mannose-6-Phosphate Receptors and Lysosome Function

Science ◽  
2012 ◽  
Vol 338 (6109) ◽  
pp. 963-967 ◽  
Author(s):  
K. McGourty ◽  
T. L. Thurston ◽  
S. A. Matthews ◽  
L. Pinaud ◽  
L. J. Mota ◽  
...  
Keyword(s):  
Retrograde Trafficking ◽  
Mannose 6 Phosphate
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