scholarly journals Formation of the Legionella Replicative Compartment at the Crossroads of Retrograde Trafficking

2017 ◽  
Vol 7 ◽  
Author(s):  
Kevin Bärlocher ◽  
Amanda Welin ◽  
Hubert Hilbi
Keyword(s):  
Retrograde Trafficking

scholarly journals Ride the wave: Retrograde trafficking becomes Ca2+ dependent with BAIAP3

2017 ◽  
Vol 216 (7) ◽  
pp. 1887-1889 ◽  
Author(s):  
Jakob B. Sørensen
Keyword(s):  
Angiogenesis Inhibitor ◽  
Secretory Vesicle ◽  
Vesicle Formation ◽  
Retrograde Trafficking ◽  
Trans Golgi Network ◽  
Cell Biol ◽  
Golgi Network

The functions of four of the five proteins in the mammalian uncoordinated-13 (Munc13) family have been identified as priming factors in SNARE-dependent exocytosis. In this issue, Zhang et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201702099) show that the fifth member, BAIAP3 (brain-specific angiogenesis inhibitor I–associated protein 3), acts in retrograde trafficking by returning secretory vesicle material to the trans-Golgi network. In its absence, secretory vesicle formation is impaired, leading to accumulation of immature vesicles, or lysosomal vesicle degradation.

scholarly journals Salmonella Inhibits Retrograde Trafficking of Mannose-6-Phosphate Receptors and Lysosome Function

Science ◽  
2012 ◽  
Vol 338 (6109) ◽  
pp. 963-967 ◽  
Author(s):  
K. McGourty ◽  
T. L. Thurston ◽  
S. A. Matthews ◽  
L. Pinaud ◽  
L. J. Mota ◽  
...  
Keyword(s):  
Retrograde Trafficking ◽  
Mannose 6 Phosphate

scholarly journals Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Harriet Crawley-Snowdon ◽  
Ji-Chun Yang ◽  
Nathan R. Zaccai ◽  
Luther J. Davis ◽  
Lena Wartosch ◽  
...  
Keyword(s):  
Cell Surface ◽  
Regulatory Network ◽  
Regulatory Proteins ◽  
X Ray ◽  
Retrograde Trafficking

Abstract VARP and TBC1D5 are accessory/regulatory proteins of retromer-mediated retrograde trafficking from endosomes. Using an NMR/X-ray approach, we determined the structure of the complex between retromer subunit VPS29 and a 12 residue, four-cysteine/Zn++ microdomain, which we term a Zn-fingernail, two of which are present in VARP. Mutations that abolish VPS29:VARP binding inhibit trafficking from endosomes to the cell surface. We show that VARP and TBC1D5 bind the same site on VPS29 and can compete for binding VPS29 in vivo. The relative disposition of VPS29s in hetero-hexameric, membrane-attached, retromer arches indicates that VARP will prefer binding to assembled retromer coats through simultaneous binding of two VPS29s. The TBC1D5:VPS29 interaction is over one billion years old but the Zn-fingernail appears only in VARP homologues in the lineage directly giving rise to animals at which point the retromer/VARP/TBC1D5 regulatory network became fully established.

scholarly journals Clathrin-independent endocytosis, retrograde trafficking, and cell polarity

2020 ◽  
Vol 65 ◽  
pp. 112-121 ◽  
Author(s):  
Massiullah Shafaq-Zadah ◽  
Estelle Dransart ◽  
Ludger Johannes
Keyword(s):  
Cell Polarity ◽  
Retrograde Trafficking

scholarly journals Microtubule Retrograde Motors and Their Role in Retroviral Transport

Viruses ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 483
Author(s):  
Gianfranco Pietrantoni ◽  
Rodrigo Ibarra-Karmy ◽  
Gloria Arriagada
Keyword(s):  
Host Cell ◽  
Reverse Transcription ◽  
Eukaryotic Cell ◽  
Chromosomal Dna ◽  
Viral Dna ◽  
Preintegration Complex ◽  
Retrograde Trafficking ◽  
Microtubule Associated Proteins ◽  
Associated Proteins ◽  
Crowded Environment

Following entry into the host cell, retroviruses generate a dsDNA copy of their genomes via reverse transcription, and this viral DNA is subsequently integrated into the chromosomal DNA of the host cell. Before integration can occur, however, retroviral DNA must be transported to the nucleus as part of a ‘preintegration complex’ (PIC). Transporting the PIC through the crowded environment of the cytoplasm is challenging, and retroviruses have evolved different mechanisms to accomplish this feat. Within a eukaryotic cell, microtubules act as the roads, while the microtubule-associated proteins dynein and kinesin are the vehicles that viruses exploit to achieve retrograde and anterograde trafficking. This review will examine the various mechanisms retroviruses have evolved in order to achieve retrograde trafficking, confirming that each retrovirus has its own strategy to functionally subvert microtubule associated proteins.

scholarly journals Role of Retrograde Trafficking in Stress Response, Host Cell Interactions, and Virulence of Candida albicans

2013 ◽  
Vol 13 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Yaoping Liu ◽  
Norma V. Solis ◽  
Clemens J. Heilmann ◽  
Quynh T. Phan ◽  
Aaron P. Mitchell ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Host Cell ◽  
Signaling Pathway ◽  
Stress Resistance ◽  
Cell Interactions ◽  
Content Type ◽  
Retrograde Trafficking ◽  
Host Cell Interactions ◽  
Vacuolar Protein ◽  
Calcineurin Signaling

ABSTRACTInSaccharomyces cerevisiae, the vacuolar protein sorting complexes Vps51/52/53/54 and Vps15/30/34/38 are essential for efficient endosome-to-Golgi complex retrograde transport. Here we investigated the function of Vps15 and Vps51, representative members of these complexes, in the stress resistance, host cell interactions, and virulence ofCandida albicans. We found thatC. albicansvps15Δ/Δ andvps51Δ/Δ mutants had abnormal vacuolar morphology, impaired retrograde protein trafficking, and dramatically increased susceptibility to a variety of stressors. These mutants also had reduced capacity to invade and damage oral epithelial cellsin vitroand attenuated virulence in the mouse model of oropharyngeal candidiasis. Proteomic analysis of the cell wall of thevps51Δ/Δ mutant revealed increased levels of the Crh11 and Utr2 transglycosylases, which are targets of the calcineurin signaling pathway. The transcript levels of the calcineurin pathway membersCHR11,UTR2,CRZ1,CNA1, andCNA2were elevated in thevps15Δ/Δ andvps51Δ/Δ mutants. Furthermore, these strains were highly sensitive to the calcineurin-specific inhibitor FK506. Also, deletion ofCHR11andUTR2further increased the stress susceptibility of these mutants. In contrast, overexpression ofCRH11andUTR2partially rescued their defects in stress resistance, but not host cell interactions. Therefore, intact retrograde trafficking inC. albicansis essential for stress resistance, host cell interactions, and virulence. Aberrant retrograde trafficking stimulates the calcineurin signaling pathway, leading to the increased expression of Chr11 and Utr2, which enablesC. albicansto withstand environmental stress.

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