scholarly journals Editorial: A New Era in Dentistry: Stem Cell-Based Approaches for Tooth and Periodontal Tissue Regeneration

2016 ◽  
Vol 7 ◽  
Author(s):  
Thimios A. Mitsiadis ◽  
Giovanna Orsini
Keyword(s):  
Stem Cell ◽  
Tissue Regeneration ◽  
Periodontal Tissue ◽  
New Era ◽  
Periodontal Tissue Regeneration

Stem cell-delivery therapeutics for periodontal tissue regeneration

Biomaterials ◽  
2012 ◽  
Vol 33 (27) ◽  
pp. 6320-6344 ◽  
Author(s):  
Fa-Ming Chen ◽  
Hai-Hua Sun ◽  
Hong Lu ◽  
Qing Yu
Keyword(s):  
Stem Cell ◽  
Tissue Regeneration ◽  
Cell Delivery ◽  
Periodontal Tissue ◽  
Stem Cell Delivery ◽  
Periodontal Tissue Regeneration

scholarly journals MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Daigaku Hasegawa ◽  
Kana Hasegawa ◽  
Hiroshi Kaneko ◽  
Shinichiro Yoshida ◽  
Hiromi Mitarai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
Stem Cell Markers ◽  
Periodontal Tissue ◽  
Differentiation Potential ◽  
Cell Markers ◽  
Pdl Cells ◽  
Periodontal Tissue Regeneration

Periodontal ligament (PDL) stem cells (PDLSCs) have been reported as a useful cell source for periodontal tissue regeneration. However, one of the issues is the difficulty of obtaining a sufficient number of PDLSCs for clinical application because very few PDLSCs can be isolated from PDL tissue of donors. Therefore, we aimed to identify a specific factor that converts human PDL cells into stem-like cells. In this study, microarray analysis comparing the gene profiles of human PDLSC lines (2-14 and 2-23) with those of a cell line with a low differentiation potential (2-52) identified the imprinted gene mesoderm-specific transcript (MEST). MEST was expressed in the cytoplasm of 2-23 cells. Knockdown of MEST by siRNA in 2-23 cells inhibited the expression of stem cell markers, such as CD105, CD146, p75NTR, N-cadherin, and NANOG; the proliferative potential; and multidifferentiation capacity for osteoblasts, adipocytes, and chondrocytes. On the other hand, overexpression of MEST in 2-52 cells enhanced the expression of stem cell markers and PDL-related markers and the multidifferentiation capacity. In addition, MEST-overexpressing 2-52 cells exhibited a change in morphology from a spindle shape to a stem cell-like round shape that was similar to 2-14 and 2-23 cell morphologies. These results suggest that MEST plays a critical role in the maintenance of stemness in PDLSCs and converts PDL cells into PDLSC-like cells. Therefore, this study indicates that MEST may be a therapeutic factor for periodontal tissue regeneration by inducing PDLSCs.

Dental tissue-derived stem cell sheet biotechnology for periodontal tissue regeneration: A systematic review

2021 ◽  
pp. 105182
Author(s):  
Fabiana Divina Magalhães ◽  
Giovanna Sarra ◽  
Giovanna Lopes Carvalho ◽  
Ana Clara Fagundes Pedroni ◽  
Márcia Martins Marques ◽  
...  
Keyword(s):  
Systematic Review ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Cell Sheet ◽  
Periodontal Tissue ◽  
Dental Tissue ◽  
Periodontal Tissue Regeneration

scholarly journals Stem cell therapies for periodontal tissue regeneration: a network meta-analysis of preclinical studies

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Qiang Li ◽  
Guangwen Yang ◽  
Jialing Li ◽  
Meng Ding ◽  
Na Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
Meta Analysis ◽  
Preclinical Studies ◽  
Periodontal Tissue ◽  
Stem Cell Therapies ◽  
Cell Therapies ◽  
Periodontal Tissue Regeneration

Abstract Background Periodontal tissue regeneration (PTR) is the ultimate goal of periodontal therapy. Currently, stem cell therapy is considered a promising strategy for achieving PTR. However, there is still no conclusive comparison that distinguishes clear hierarchies among different kinds of stem cells. Methods A systematic review and network meta-analysis (NMA) was performed using MEDLINE (via PubMed), EMBASE, and Web of Science up to February 2020. Preclinical studies assessing five types of stem cells for PTR were included; the five types of stem cells included periodontal ligament-derived stem cells (PDLSCs), bone marrow-derived stem cells (BMSCs), adipose tissue-derived stem cells (ADSCs), dental pulp-derived stem cells (DPSCs), and gingival-derived stem cells (GMSCs). The primary outcomes were three histological indicators with continuous variables: newly formed alveolar bone (NB), newly formed cementum (NC), and newly formed periodontal ligament (NPDL). We performed pairwise meta-analyses using a random-effects model and then performed a random-effects NMA using a multivariate meta-analysis model. Results Sixty preclinical studies assessing five different stem cell-based therapies were identified. The NMA showed that in terms of NB, PDLSCs (standardized mean difference 1.87, 95% credible interval 1.24 to 2.51), BMSCs (1.88, 1.17 to 2.59), and DPSCs (1.69, 0.64 to 2.75) were statistically more efficacious than cell carriers (CCs). In addition, PDLSCs were superior to GMSCs (1.49, 0.04 to 2.94). For NC, PDLSCs (2.18, 1.48 to 2.87), BMSCs (2.11, 1.28 to 2.94), and ADSCs (1.55, 0.18 to 2.91) were superior to CCs. For NPDL, PDLSCs (1.69, 0.92 to 2.47) and BMSCs (1.41, 0.56 to 2.26) were more efficacious than CCs, and PDLSCs (1.26, 0.11 to 2.42) were superior to GMSCs. The results of treatment hierarchies also demonstrated that the two highest-ranked interventions were PDLSCs and BMSCs. Conclusion PDLSCs and BMSCs were the most effective and well-documented stem cells for PTR among the five kinds of stem cells evaluated in this study, and there was no statistical significance between them. To translate the stem cell therapies for PTR successfully in the clinic, future studies should utilize robust experimental designs and reports.

scholarly journals Surgical Management of Horizontal Root Fracture with Platelet Rich Fibrin (PRF) Placement: Case Report of Two Cases

2013 ◽  
Vol 01 (02) ◽  
pp. 125-128
Author(s):  
Parul Bansal ◽  
Kalpana Kanyal ◽  
Vineeta Nikhil
Keyword(s):  
Case Report ◽  
Tissue Regeneration ◽  
Surgical Approach ◽  
Permanent Teeth ◽  
Periodontal Tissue ◽  
Root Fracture ◽  
Platelet Rich Fibrin ◽  
Periodontal Tissue Regeneration ◽  
Root Fractures ◽  
Horizontal Root Fracture

AbstractRadicular fractures in permanent teeth are uncommon injuries among dental traumas, being only 0.5 - 7% of the cases. Horizontal root fractures can be managed endodontically or combined endodontic and surgical approach. Treatment varies according to the displacement and vitality of the fragments. This paper presents a case report of two cases of horizontal root fracture, present between the middle and apical third of central incisors, which were managed by combined endodontic and surgical approach, while in second case it was followed by PRF placement to facilitate osteoinduction and periodontal tissue regeneration.

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