scholarly journals Corrigendum: Taheebo Polyphenols Attenuate FFA-Induced Inflammation in Murine and Human Macrophage Cell Lines As Inhibitor of COX-2

2018 ◽  
Vol 5 ◽  
Author(s):  
Sihui Ma ◽  
Koichi Yada ◽  
Hyunjin Lee ◽  
Youichi Fukuda ◽  
Akira Iida ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Macrophage ◽  
Macrophage Cell ◽  
Cox 2

The immunosuppressive effects of a novel recombinant LipQ (Rv2485c) protein of Mycobacterium tuberculosis on human macrophage cell lines

2017 ◽  
Vol 107 ◽  
pp. 361-367 ◽  
Author(s):  
Anjani Kumar ◽  
Manisha ◽  
Gurkamaljit Kaur Sangha ◽  
Anju Shrivastava ◽  
Jagdeep Kaur
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cell Lines ◽  
Human Macrophage ◽  
Macrophage Cell

scholarly journals Macrophage cell lines and murine infection by Salmonella Typhi L-form bacteria

2021 ◽  
Author(s):  
Debayan Ganguli ◽  
Swarnali Chakraborty ◽  
Suparna Chakraborty ◽  
Ananda Pal ◽  
Animesh Gope ◽  
...  
Keyword(s):  
Cell Wall ◽  
Antibiotic Resistance ◽  
Cell Lines ◽  
Antibiotic Treatment ◽  
Disease Transmission ◽  
Parental Strain ◽  
Pathogenic Bacteria ◽  
Human Macrophage ◽  
Macrophage Cell ◽  
Drug Concentrations

AbstractAntibiotic resistance of pathogenic bacteria has emerged as a major threat to public health worldwide. While stable resistance due to the acquisition of genomic mutations or plasmids carrying antibiotic-resistance genes is well-established, much less is known about the temporary and reversible resistance induced by antibiotic treatment, such as the one due to treatment with bacterial cell-wall inhibiting antibiotics like ampicillin. Typically, ampicillin concentration in the blood and other tissues gradually increases over time after initiation of the treatment. As a result, the bacterial population is exposed to a concentration gradient of ampicillin. This is different from in vitro drug testing where the organism is exposed to fixed drug concentrations from the beginning till the end. To mimic the mode of antibiotic exposure of microorganisms in the tissues, we cultured the wild type, ampicillin-sensitive Salmonella Typhi Ty2 strain (S. Typhi Ty2) in the presence of increasing concentrations of ampicillin over a period of 14 days. This resulted in the development of a strain that exhibited several features of the so-called L-form of bacteria, such as the absence of cell wall, altered shape and slower growth rate compared with the parental strain. Studies on the pathogenesis of S. Typhi L-form showed efficient infection of the murine and human macrophage cell lines. More importantly, S. Typhi L-form was also able to establish infection in a mouse model to the extent comparable to its parental strain. These results suggested that L-form generation following initiation of antibiotic treatment could lead to drug escape of S. Typhi and direct spread to new cells (macrophages), which sustain the infection. Oral infection by the L-form bacteria underscores the potential of rapid disease transmission through faeco-oral route, highlighting the need for new approaches to decrease the reservoir of infection.

7C3, A Marker for the Differentiation of Human Macrophage Cell Lines

1987 ◽  
Vol 42 (5) ◽  
pp. 491-497 ◽  
Author(s):  
Steven H. Zuckerman ◽  
Julia Tang ◽  
Bruce D. Gitter ◽  
Maurice E. Scheetz
Keyword(s):  
Cell Lines ◽  
Human Macrophage ◽  
Macrophage Cell

scholarly journals Poly-dispersed-acid-functionalized-single-walled carbon nanotubes (AF-SWCNTs) are potent inhibitor of BCG induced inflammatory response in macrophage cell lines

2020 ◽  
Author(s):  
Deepika Bhardwaj ◽  
Rajiv K. Saxena
Keyword(s):  
Carbon Nanotubes ◽  
Inflammatory Response ◽  
Cell Lines ◽  
Single Walled Carbon Nanotubes ◽  
Transcriptional Level ◽  
Macrophage Cell ◽  
Single Walled Carbon ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Walled Carbon Nanotubes

AbstractPresent study is focussed on the modulation of Mycobacterium bovis BCG induced inflammatory response by poly-dispersed acid-functionalized single-walled carbon nanotubes (AF-SWCNTs) in macrophages. Flow cytometric and confocal microscopy studies indicated that both BCG and AF-SWCNTs were efficiently internalized by RAW 264.7 and MH-S macrophage cell lines and were essentially localized in the cytoplasmic area. The results indicated strong antioxidant activity of AF-SWCNTs in mitigating BCG induced oxidative and nitrosative stress. We also found a marked decline in expression of BCG induced pro-inflammatory genes like COX-2, iNOS, TNF-α, IL-6 and IL-1β on treatment with AF-SWCNTs at transcriptional level. Decline in expression of BCG induced COX-2 by AF-SWCNTs was also confirmed at protein level using Western blotting. Anti-inflammatory activity of AF-SWCNTs was further validated by our results showing that AF-SWCNTs treatment induced a precipitous decline in BCG induced release of Matrix Metalloproteinases MMP-2 and MMP-9 by macrophage cell lines, by using Gelatin zymography. Taken together, our results demonstrate potent anti-inflammatory role of AF-SWCNTs in alleviating BCG induced inflammation.

New Ferrocene Compounds as Selective Cyclooxygenase (COX-2) Inhibitors: Design, Synthesis, Cytotoxicity and Enzyme-inhibitory Activity

2018 ◽  
Vol 18 (2) ◽  
pp. 295-301 ◽  
Author(s):  
Shabnam Farzaneh ◽  
Elnaz Zeinalzadeh ◽  
Bahram Daraei ◽  
Soraya Shahhosseini ◽  
Afshin Zarghi
Keyword(s):  
Cell Lines ◽  
Inhibitory Activity ◽  
Fibroblast Cell ◽  
Breast Cancer Cell Lines ◽  
Ferrocene Derivatives ◽  
Cox 2 ◽  
Cytotoxicity Effects ◽  
Cox 2 Inhibitors ◽  
Mcf 7

Background: Due to the astonishing properties of ferrocene and its derivatives, it has a broad application in diverse areas. Numerous ferrocene derivatives demonstrated anti-proliferative activity. Also COX-2, as a key isoenzyme for production of prostaglandins, is frequently overexpressed in various cancers. It is now recognized that COX-2 over expression promotes tumorigenic functions which can be suppressed by COX-2 inhibitors, a phenomenon useful for the preventing of tumor progression. The combination of COX-2 inhibitors with other anti-cancer or cancer prevention drugs may reduce their side effects in future cancer prevention and treatment. Objective: Owing to high anticancer potential of ferrocene derivatives and considerable COX-2 inhibitory and cytotoxicity effects of our previously synthesized chalcones, we decided to incorporate the ferrocenyl moiety into appropriate COX-2 inhibitor chalcone based scaffold, to evaluate COX-2 inhibitory activity as well as anticancer activities. Methods: Chalcones were synthesized via clasien-schmidt condensation of methylsulfonyl aldehyde and acetyl ferrocene. Further different amines with solvent free and ultra sound condition were reacted with chalcones to have different 1-ferrocenyl-3-amino carbonyl compounds. Docking study was carried out with Auto Dock vina software. All the newly-synthesized compounds were evaluated for their cyclooxygenase-2 (COX-2) inhibitory activity using chemiluminescent enzyme assays as well as cytotoxicity activity against MCF-7 and T47D and fibroblast cell lines by MTT assay. Results: In vitro COX-1/COX-2 inhibition studies demonstrated that all compounds were selective inhibitors of the COX-2 isozyme with IC50 values in the highly potent 0.05-0.12 µM range, and COX-2 selectivity indexes (SI) in the 148.3-313.7 range. These results indicated that either potency or selectivity of COX-2 inhibitory activity was affected by the nature and size of the substituents on C-3 of propane-1-one. Also anti-proliferative and toxicity activities of synthesized compounds against breast cancer cell lines MCF-7 and T47D and fibroblast cell lines showed that the synthesized compounds had mild to moderate cytotoxicity against MCT7 and T47D breast cancer cell lines at 10 µM concentration. In vitro COX-1/COX-2 inhibition studies and anticancer activity against MCF-7, identified 1-ferrocenyl-3-(4-methylsulfonylphenyl) propen-1-one as a potent compound (IC50 COX-2 = 0.05 µM, MCF-7: % inhibition (at concentration of 10 µM) = 32.7%), and also 1-ferrocenyl-3- (propan-1-amine)-3-(4-methylsulfonylphenyl) propan-1-one showed the most selectivity on COX-2 inhibition (selectivity index= 313.7). Conclusion: A novel group of ferrocene compounds, possessing a methyl sulfonyl COX-2 pharmacophore were synthesized to investigate the effect of different substituents on selectivity and potency of COX-2 inhibitory activity and their cytotoxicity effects. This study indicates that 1-ferrocenyl-3-amino carbonyl compounds having ferrocene motif and methyl sulfonyl COX-2 pharmacophore is a suitable scaffold to design COX-2 inhibitors and anti-cancer agents.

scholarly journals Bio-engineered palladium nanoparticles: model for risk assessment study of automotive particulate pollution on macrophage cell lines

RSC Advances ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 1850-1861
Author(s):  
Aarzoo ◽  
Saba Naqvi ◽  
Nidhi Bharal Agarwal ◽  
Manoj P. Singh ◽  
M. Samim
Keyword(s):  
Risk Assessment ◽  
Cell Lines ◽  
Palladium Nanoparticles ◽  
Macrophage Cell ◽  
Particulate Pollution ◽  
Assessment Study

The surge in vehicular activity in densely populated areas has led to an increased concentration of airborne palladium nanoparticles (PdNPs) in the environment.

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