Uptake and Protection against Oxidative Stress by Estrogen Esters in THP-1 Human Macrophage Cell Lines

2001 ◽  
Vol 51 (2) ◽  
pp. 81-84 ◽  
Author(s):  
W. Abplanalp ◽  
M.T. Ravi Subbiah
Keyword(s):  
Oxidative Stress ◽  
Cell Lines ◽  
Human Macrophage ◽  
Macrophage Cell

The immunosuppressive effects of a novel recombinant LipQ (Rv2485c) protein of Mycobacterium tuberculosis on human macrophage cell lines

2017 ◽  
Vol 107 ◽  
pp. 361-367 ◽  
Author(s):  
Anjani Kumar ◽  
Manisha ◽  
Gurkamaljit Kaur Sangha ◽  
Anju Shrivastava ◽  
Jagdeep Kaur
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cell Lines ◽  
Human Macrophage ◽  
Macrophage Cell

scholarly journals Macrophage cell lines and murine infection by Salmonella Typhi L-form bacteria

2021 ◽  
Author(s):  
Debayan Ganguli ◽  
Swarnali Chakraborty ◽  
Suparna Chakraborty ◽  
Ananda Pal ◽  
Animesh Gope ◽  
...  
Keyword(s):  
Cell Wall ◽  
Antibiotic Resistance ◽  
Cell Lines ◽  
Antibiotic Treatment ◽  
Disease Transmission ◽  
Parental Strain ◽  
Pathogenic Bacteria ◽  
Human Macrophage ◽  
Macrophage Cell ◽  
Drug Concentrations

AbstractAntibiotic resistance of pathogenic bacteria has emerged as a major threat to public health worldwide. While stable resistance due to the acquisition of genomic mutations or plasmids carrying antibiotic-resistance genes is well-established, much less is known about the temporary and reversible resistance induced by antibiotic treatment, such as the one due to treatment with bacterial cell-wall inhibiting antibiotics like ampicillin. Typically, ampicillin concentration in the blood and other tissues gradually increases over time after initiation of the treatment. As a result, the bacterial population is exposed to a concentration gradient of ampicillin. This is different from in vitro drug testing where the organism is exposed to fixed drug concentrations from the beginning till the end. To mimic the mode of antibiotic exposure of microorganisms in the tissues, we cultured the wild type, ampicillin-sensitive Salmonella Typhi Ty2 strain (S. Typhi Ty2) in the presence of increasing concentrations of ampicillin over a period of 14 days. This resulted in the development of a strain that exhibited several features of the so-called L-form of bacteria, such as the absence of cell wall, altered shape and slower growth rate compared with the parental strain. Studies on the pathogenesis of S. Typhi L-form showed efficient infection of the murine and human macrophage cell lines. More importantly, S. Typhi L-form was also able to establish infection in a mouse model to the extent comparable to its parental strain. These results suggested that L-form generation following initiation of antibiotic treatment could lead to drug escape of S. Typhi and direct spread to new cells (macrophages), which sustain the infection. Oral infection by the L-form bacteria underscores the potential of rapid disease transmission through faeco-oral route, highlighting the need for new approaches to decrease the reservoir of infection.

7C3, A Marker for the Differentiation of Human Macrophage Cell Lines

1987 ◽  
Vol 42 (5) ◽  
pp. 491-497 ◽  
Author(s):  
Steven H. Zuckerman ◽  
Julia Tang ◽  
Bruce D. Gitter ◽  
Maurice E. Scheetz
Keyword(s):  
Cell Lines ◽  
Human Macrophage ◽  
Macrophage Cell

scholarly journals Oxyradical stress increases the biosynthesis of 2-arachidonoylglycerol: involvement of NADPH oxidase

2016 ◽  
Vol 311 (6) ◽  
pp. C960-C974 ◽  
Author(s):  
Anberitha T. Matthews ◽  
Jung Hwa Lee ◽  
Abdolsamad Borazjani ◽  
Lee C. Mangum ◽  
Xiang Hou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nadph Oxidase ◽  
Cell Lines ◽  
Oxidized Ldl ◽  
Signal Transduction Pathway ◽  
Dependent Manner ◽  
Macrophage Cell ◽  
Biosynthetic Activity ◽  
Diacylglycerol Lipase ◽  
Cytosolic Phospholipase

NADPH oxidase (Nox)-derived oxyradicals contribute to atherosclerosis by oxidizing low-density lipoproteins (LDL), leading to their phagocytosis by vascular macrophages. Endocannabinoids, such as 2-arachidonoylglycerol (2-AG), might be an important link between oxidative stress and atherosclerosis. We hypothesized that 2-AG biosynthesis in macrophages is enhanced following ligation of oxidized LDL by scavenger receptors via a signal transduction pathway involving Nox-derived ROS that activates diacylglycerol lipase-β (DAGL-β), the 2-AG biosynthetic enzyme. To test this idea, we challenged macrophage cell lines and murine primary macrophages with a xanthine oxidase system or with nonphysiological and physiological Nox stimulants [phorbol 12-myristate 13-acetate (PMA) and arachidonic acid (AA)]. Each stressor increased cellular superoxide levels and enhanced 2-AG biosynthetic activity in a Nox-dependent manner. Levels of cytosolic phospholipase A2-dependent AA metabolites (eicosanoids) in primary macrophages were also dependent on Nox-mediated ROS. In addition, 2-AG levels in DAGL-β-overexpressing COS7 cells were attenuated by inhibitors of Nox and DAGL-β. Furthermore, ROS induced by menadione (a redox cycling agent) or PMA could be partially attenuated by the cannabinoid 1/2 receptor agonist (WIN 55,212-2). Finally, cells that overexpress Nox2 components (Phox-COS7) synthesized larger amounts of 2-AG compared with the parental COS7 cells. Together, the results suggest a positive correlation between heightened oxygen radical flux and 2-AG biosynthesis in macrophage cell lines and primary macrophages. Because of the antioxidant and anti-inflammatory effects associated with 2-AG, the increased levels of this bioactive lipid might be an adaptive response to oxidative stress. Thus oxyradical stress may be counteracted by the enhanced endocannabinoid tone.

scholarly journals Corrigendum: Taheebo Polyphenols Attenuate FFA-Induced Inflammation in Murine and Human Macrophage Cell Lines As Inhibitor of COX-2

2018 ◽  
Vol 5 ◽  
Author(s):  
Sihui Ma ◽  
Koichi Yada ◽  
Hyunjin Lee ◽  
Youichi Fukuda ◽  
Akira Iida ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Macrophage ◽  
Macrophage Cell ◽  
Cox 2

Synthesis and Anticancer Activity of 9-O-Pyrazole Alkyl Substituted Berberine Derivatives

2019 ◽  
Vol 18 (11) ◽  
pp. 1639-1648 ◽  
Author(s):  
Daipeng Xiao ◽  
Fen He ◽  
Dongming Peng ◽  
Min Zou ◽  
Junying Peng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Molecular Docking ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Human Lung Cancer ◽  
Drug Candidate ◽  
Molecular Docking Study ◽  
Protective Function ◽  
Anti Cancer ◽  
Cancer Activity

Background: Berberine (BBR), an isoquinoline plant alkaloid isolated from plants such as Coptis chinensis and Hydrastis canadensis, own multiple pharmacological activities. Objective: In this study, seven BBR derivatives were synthesized and their anticancer activity against HeLa cervical and A549 human lung cancer cell lines were evaluated in vitro. Methods: The anti-cancer activity was measured by MTT assay, and apoptosis was demonstrated by the annexin V-FITC/PI staining assay. The intracellular oxidative stress was investigated through DCFH-DA assay. The molecular docking study was carried out in molecular operating environment (MOE). Results: Compound B3 and B5 showed enhanced anti-cancer activity compared with BBR, the IC50 for compound B3 and B5 were significantly lower than BBR, and compound B3 at the concentration of 64 or 128 µM induced apoptosis in HeLa and A549 cell lines. The reactive oxygen species (ROS) was generated in both cell lines when treated with 100 µM of all the compounds, and compound B3 and B5 induced higher activity in the generation of ROS, while compound B3 exhibited the highest activity, these results are in accordance with the cytotoxicity results, indicating the cytotoxicity were mostly generated from the oxidative stress. In addition, molecular docking analysis showed that compound B3 had the greatest affinity with Hsp90. Upon binding, the protective function of Hsp90 was lost, which might explain its higher cytotoxicity from molecular interaction aspect. Conclusion: All the results demonstrated that compound B3 and B5 showed significantly higher anti-cancer ability than BBR, and compound B3 is a promising anticancer drug candidate.

Artepillin C Induces Selective Oxidative Stress and Inhibits Migration and Invasion in a Comprehensive Panel of Human Cervical Cancer Cell Lines

2019 ◽  
Vol 18 (12) ◽  
pp. 1750-1760 ◽  
Author(s):  
Raquel P. Souza ◽  
Patrícia S. Bonfim-Mendonça ◽  
Gabrielle M.Z.F. Damke ◽  
Analine R.B. de-Assis Carvalho ◽  
Bianca A. Ratti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Cell Lines ◽  
Migration And Invasion ◽  
Epithelial Cell Line ◽  
Cellular Viability ◽  
Hpv 16 ◽  
Anticancer Properties ◽  
Artepillin C ◽  
Human Cervical Cancer

Background: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is the main bioactive component of Brazilian green propolis, and possesses, among other things, anticancer properties. However, to the best of our knowledge, there are no studies of artepillin C in cervical cancer. Method: To explore a new therapeutic candidate for cervical cancer, we have evaluated the effects of artepillin C on cellular viability in a comprehensive panel of human cervical cancer-derived cell lines including HeLa (human papillomavirus/HPV 18-positive), SiHa (HPV 16-positive), CaSki (HPV 16- and 18-positive) and C33A (HPV-negative) cells compared to a spontaneously immortalized human epithelial cell line (HaCaT). Results: Our results demonstrated that artepillin C had a selective effect on cellular viability and could induce apoptosis possibly by intrinsic pathway, likely a result of oxidative stress, in all cancer-derived cell lines but not in HaCaT. Additionally, artepillin C was able to inhibit the migration and invasion of cancer cells. Conclusion: Thus, artepillin C appears to be a promising new candidate as an anticancer drug for cervical cancer induced by different HPV types.

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