scholarly journals Adaptation of Carbon Source Utilization Patterns of Geobacter metallireducens During Sessile Growth

2020 ◽  
Vol 11 ◽  
Author(s):  
Sviatlana Marozava ◽  
Juliane Merl-Pham ◽  
Hubert Müller ◽  
Rainer U. Meckenstock
Keyword(s):  
Carbon Source ◽  
Carbon Source Utilization ◽  
Geobacter Metallireducens ◽  
Utilization Patterns

scholarly journals Attached and Unattached Microbial Communities in a Simulated Basalt Aquifer under Fracture- and Porous-Flow Conditions

2001 ◽  
Vol 67 (6) ◽  
pp. 2799-2809 ◽  
Author(s):  
R. Michael Lehman ◽  
Frederick S. Colwell ◽  
Greg A. Bala
Keyword(s):  
Carbon Source ◽  
Acid Methyl Ester ◽  
Packed Columns ◽  
Most Probable Number ◽  
Total Biomass ◽  
Carbon Source Utilization ◽  
Probable Number ◽  
Geologic Medium ◽  
Utilization Patterns

ABSTRACT Bench scale column studies were used to examine the partitioning of microorganisms between groundwater and a geologic medium and to examine the effect of hydrogeology (i.e., porous- versus fracture-flow) on organism partitioning. Replicated columns were constructed with intact basalt core segments that contained natural fractures and with the same basalt crushed into particles. The columns were perfused with groundwater, and upon reaching a steady state, the columns were sacrificed and the attached and unattached communities were analyzed by multiple approaches. The analyses included the total number of cells, the phylogenetic affiliation of the cells (i.e., the α, β, and γ subclasses of the class Proteobacteria and gram positives with high G+C DNA content) by fluorescent in situ hybridization (FISH), number and taxonomic affiliation by fatty acid methyl ester profiles of culturable heterotrophs, most-probable-number estimates of methanotrophs and phenol oxidizers, and whole-community sole carbon source utilization patterns from Biolog GN microplates. In the packed columns, about 99% of the total biomass (per cubic centimeter of porous medium) was attached to the geologic medium. Lack of equitable units precluded a comparison of attached and unattached biomasses in the fractured columns where the attached biomass was expressed per unit of surface area. Compositional differences in the attached and unattached communities were evidenced by (i) the recovery ofPseudomonas stutzeri, an Enterococcus sp., andBacillus psychrophilus from the groundwater and not from the basalt, (ii) differences between community carbon source utilization patterns, and (iii) the relative abundances of different phylogenetic groups estimated by FISH in both column types. In the packed columns, attached communities were depleted of members of the α- and β-Proteobacteria subclasses in comparison to those in the corresponding groundwater. In the fractured columns, attached communities were enriched in gram-positive Bacteriaand γ-Proteobacteria and depleted of β-Proteobacteria, in comparison to those in the corresponding groundwater. Segregation of populations and their activities, possibly modified by attachment to geologic media, may influence contaminant fate and transport in the subsurface and impact other in situ applications.

scholarly journals Use of Phospholipid Fatty Acids and Carbon Source Utilization Patterns To Track Microbial Community Succession in Developing Compost

1998 ◽  
Vol 64 (10) ◽  
pp. 4062-4064 ◽  
Author(s):  
Lynne Carpenter-Boggs ◽  
Ann C. Kennedy ◽  
John P. Reganold
Keyword(s):  
Fatty Acids ◽  
Microbial Community ◽  
Carbon Source ◽  
Phospholipid Fatty Acid ◽  
Community Succession ◽  
Carbon Source Utilization ◽  
Utilization Patterns ◽  
Common Plant ◽  
Microbial Community Succession ◽  
Over Time

ABSTRACT Carbon source utilization and phospholipid fatty acid analyses were used to track the rapidly changing microbial community in composting dairy waste. Microbial abilities to utilize common plant sugars increased during composting. Community phospholipid profiles changed significantly over time. Phospholipids suggested the presence of more thermophiles and fewer bacteria with continued compost development.

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