scholarly journals Amastigote Synapse: The Tricks of Trypanosoma cruzi Extracellular Amastigotes

2018 ◽  
Vol 9 ◽  
Author(s):  
Alexis Bonfim-Melo ◽  
Eden R. Ferreira ◽  
Pilar T. V. Florentino ◽  
Renato A. Mortara
Keyword(s):  
Trypanosoma Cruzi ◽  
Extracellular Amastigotes

scholarly journals Lysosomal exocytosis: An important event during invasion of lamp deficient cells by extracellular amastigotes of Trypanosoma cruzi

2009 ◽  
Vol 384 (2) ◽  
pp. 265-269 ◽  
Author(s):  
Emanuelle Baldo Gaspar ◽  
Renato Arruda Mortara ◽  
Luciana O. Andrade ◽  
Claudio Vieira da Silva
Keyword(s):  
Trypanosoma Cruzi ◽  
Extracellular Amastigotes

scholarly journals ERM Proteins Play Distinct Roles in Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi

2017 ◽  
Vol 8 ◽  
Author(s):  
Éden R. Ferreira ◽  
Alexis Bonfim-Melo ◽  
Esteban M. Cordero ◽  
Renato A. Mortara
Keyword(s):  
Trypanosoma Cruzi ◽  
Cell Invasion ◽  
Erm Proteins ◽  
Extracellular Amastigotes

The induction of Trypanosoma cruzi trypomastigote to amastigote transformation by low pH

Parasitology ◽  
1995 ◽  
Vol 110 (5) ◽  
pp. 547-554 ◽  
Author(s):  
S. Tomlinson ◽  
F. Vandekerckhove ◽  
U. Frevert ◽  
V. Nussenzweig
Keyword(s):  
Trypanosoma Cruzi ◽  
Molecular Mechanisms ◽  
Low Ph ◽  
Critical Stage ◽  
Intracellular Amastigotes ◽  
Electron Microscopical Analysis ◽  
Physiological Relevance ◽  
Electron Microscopical ◽  
Extracellular Amastigotes

SUMMARYFollowing cell invasion, Trypanosoma cruzi trypomastigotes transform into amastigotes, which are the mammalian replicative forms of the parasite. Although amastigotes represent a critical stage in the life-cycle of T. cruzi, little is known of the factors controlling trypomastigote to amastigote transformation. Kanbera et al. (1990) observed that exposure of trypomastigotes to acidic pH induced their transformation into rounded forms resembling amastigotes. We confirm their observation and, using two strains of T. cruzi, establish that these transformants are ultrastructurally and biochemically indistinguishable from natural amastigotes. Incubation of trypomastigotes in medium at pH 5·0 for 2 h was sufficient to trigger their transformation into forms resembling amastigotes. Electron microscopical analysis confirmed that the kinetoplast structure, and general morphological features of the acid-induced, extracellular amastigotes were indistinguishable from those of intracellular-derived amastigotes. The extracellular transformation was accompanied by the acquisition of the stage-specific surface antigen of the naturally transformed amastigotes (Ssp-4), and loss of a stagespecific trypomastigote antigen (Ssp-3). Trypomastigotes incubated at neutral pH did not transform into amastigotes, and did not acquire the Ssp-4 epitope or lose the Ssp-3 epitope. Finally, acid-induced amastigotes subsequently incorporated [3H]thymidine into their DNA, indicating that the important replicative property of intracellular amastigotes is also exhibited by these in vitro transformants. This effect of low pH appears to be of physiological relevance, and acid-induced extracellular transformation appears to represent a valid experimental technique for studies of the molecular mechanisms involved in the differentiation process.

Trypanosoma cruzi extracellular amastigotes engage Rac1 and Cdc42 to invade RAW macrophages

2021 ◽  
pp. 104837
Author(s):  
Camila Macedo Medina ◽  
Éden Ramalho Ferreira ◽  
Bruno Souza Bonifácio ◽  
Renato Arruda Mortara ◽  
Alexis Bonfim-Melo
Keyword(s):  
Trypanosoma Cruzi ◽  
Extracellular Amastigotes

scholarly journals Trypanosoma cruzi extracellular amastigotes selectively trigger the PI3K/Akt and Erk pathways during HeLa cell invasion

2019 ◽  
Vol 21 (10) ◽  
pp. 485-489 ◽  
Author(s):  
Bianca Lima Ferreira ◽  
Éden Ramalho Ferreira ◽  
Alexis Bonfim-Melo ◽  
Renato Arruda Mortara ◽  
Diana Bahia
Keyword(s):  
Trypanosoma Cruzi ◽  
Hela Cell ◽  
Cell Invasion ◽  
Extracellular Amastigotes

scholarly journals A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes

2018 ◽  
Vol 9 ◽  
Author(s):  
Pilar T. V. Florentino ◽  
Fernando Real ◽  
Cristina M. Orikaza ◽  
Julia P. C. da Cunha ◽  
Francisca N. L. Vitorino ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Invasion ◽  
Carbohydrate Moiety ◽  
Host Cell Invasion ◽  
Extracellular Amastigotes
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