scholarly journals ERM Proteins Play Distinct Roles in Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi

2017 ◽  
Vol 8 ◽  
Author(s):  
Éden R. Ferreira ◽  
Alexis Bonfim-Melo ◽  
Esteban M. Cordero ◽  
Renato A. Mortara
Keyword(s):  
Trypanosoma Cruzi ◽  
Cell Invasion ◽  
Erm Proteins ◽  
Extracellular Amastigotes

scholarly journals Trypanosoma cruzi extracellular amastigotes selectively trigger the PI3K/Akt and Erk pathways during HeLa cell invasion

2019 ◽  
Vol 21 (10) ◽  
pp. 485-489 ◽  
Author(s):  
Bianca Lima Ferreira ◽  
Éden Ramalho Ferreira ◽  
Alexis Bonfim-Melo ◽  
Renato Arruda Mortara ◽  
Diana Bahia
Keyword(s):  
Trypanosoma Cruzi ◽  
Hela Cell ◽  
Cell Invasion ◽  
Extracellular Amastigotes

scholarly journals A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes

2018 ◽  
Vol 9 ◽  
Author(s):  
Pilar T. V. Florentino ◽  
Fernando Real ◽  
Cristina M. Orikaza ◽  
Julia P. C. da Cunha ◽  
Francisca N. L. Vitorino ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Invasion ◽  
Carbohydrate Moiety ◽  
Host Cell Invasion ◽  
Extracellular Amastigotes

scholarly journals Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes

Glycobiology ◽  
2013 ◽  
Vol 24 (2) ◽  
pp. 179-184 ◽  
Author(s):  
F. C. Machado ◽  
L. Cruz ◽  
A. A. da Silva ◽  
M. C. Cruz ◽  
R. A. Mortara ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Cell Invasion ◽  
Intracellular Trafficking ◽  
Galectin 3 ◽  
Extracellular Amastigotes

scholarly journals Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion

2011 ◽  
Vol 106 (8) ◽  
pp. 1014-1016 ◽  
Author(s):  
Maria Cecília Fernandes ◽  
Leonardo Rodrigues de Andrade ◽  
Norma Windsor Andrews ◽  
Renato Arruda Mortara
Keyword(s):  
Trypanosoma Cruzi ◽  
Hela Cell ◽  
Cell Invasion

scholarly journals Inhibition of Host Cell Lysosome Spreading by Trypanosoma cruzi Metacyclic Stage-Specific Surface Molecule gp90 Downregulates Parasite Invasion

2017 ◽  
Vol 85 (9) ◽  
Author(s):  
João Paulo Ferreira Rodrigues ◽  
Guilherme Hideki Takahashi Sant'ana ◽  
Maria Aparecida Juliano ◽  
Nobuko Yoshida
Keyword(s):  
Trypanosoma Cruzi ◽  
Hela Cell ◽  
Specific Surface ◽  
Host Cell ◽  
Cell Invasion ◽  
Target Cell ◽  
Content Type ◽  
Terminal Domain ◽  
Successful Infection

ABSTRACT Successful infection by Trypanosoma cruzi, the agent of Chagas' disease, is critically dependent on host cell invasion by metacyclic trypomastigote (MT) forms. Two main metacyclic stage-specific surface molecules, gp82 and gp90, play determinant roles in target cell invasion in vitro and in oral T. cruzi infection in mice. The structure and properties of gp82, which is highly conserved among T. cruzi strains, are well known. Information on gp90 is still rather sparse. Here, we attempted to fill that gap. gp90, purified from poorly invasive G strain MT and expressing gp90 at high levels, inhibited HeLa cell lysosome spreading and the gp82-mediated internalization of a highly invasive CL strain MT expressing low levels of a diverse gp90 molecule. A recombinant protein containing the conserved C-terminal domain of gp90 exhibited the same properties as the native G strain gp90: it counteracted the host cell lysosome spreading induced by recombinant gp82 and exhibited an inhibitory effect on HeLa cell invasion by CL strain MT. Assays to identify the gp90 sequence associated with the property of downregulating MT invasion, using synthetic peptides spanning the gp90 C-terminal domain, revealed the sequence GVLYTADKEW. These data, plus the findings that lysosome spreading was induced upon HeLa cell interaction with CL strain MT, but not with G strain MT, and that in mixed infection CL strain MT internalization was inhibited by G strain MT, suggest that the inhibition of target cell lysosome spreading is the mechanism by which the gp90 molecule exerts its downregulatory role.

Sign in / Sign up

Export Citation Format

Share Document