scholarly journals A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes

2018 ◽  
Vol 9 ◽  
Author(s):  
Pilar T. V. Florentino ◽  
Fernando Real ◽  
Cristina M. Orikaza ◽  
Julia P. C. da Cunha ◽  
Francisca N. L. Vitorino ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Invasion ◽  
Carbohydrate Moiety ◽  
Host Cell Invasion ◽  
Extracellular Amastigotes

scholarly journals Host cell invasion and oral infection by Trypanosoma cruzi strains of genetic groups TcI and TcIV from chagasic patients

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Fernando Yukio Maeda ◽  
Tatiana Mordente Clemente ◽  
Silene Macedo ◽  
Cristian Cortez ◽  
Nobuko Yoshida
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Invasion ◽  
Oral Infection ◽  
Host Cell Invasion ◽  
Genetic Groups

scholarly journals Expression and Cellular Localization of Molecules of the gp82 Family in Trypanosoma cruzi Metacyclic Trypomastigotes

2007 ◽  
Vol 75 (7) ◽  
pp. 3264-3270 ◽  
Author(s):  
Vanessa D. Atayde ◽  
Mauro Cortez ◽  
Renata Souza ◽  
José Franco da Silveira ◽  
Nobuko Yoshida
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Invasion ◽  
Cellular Localization ◽  
Polyclonal Antibodies ◽  
Microscopic Analysis ◽  
Cdna Clones ◽  
Host Cell Invasion ◽  
Two Dimensional Gel Electrophoresis ◽  
Surface Localization

ABSTRACT A member of the Trypanosoma cruzi gp82 family, expressed on metacyclic trypomastigote surface and identified by monoclonal antibody (MAb) 3F6, plays a key role in host cell invasion. Apart from the gp82 defined by MAb 3F6, no information is available on members of this protein family. From cDNA clones encoding gp82 proteins sharing 59.1% sequence identity, we produced the recombinant proteins J18 and C03, the former containing and the latter lacking the epitope for MAb 3F6. Polyclonal antibodies to J18 and C03 proteins were generated and used, along with MAb 3F6, to analyze the expression and cellular localization of gp82 family members in metacyclic forms of CL and G strains, which belong to highly divergent genetic groups. By two-dimensional gel electrophoresis and immunoblotting, molecules of 82 to 86 kDa, focusing at pH 4.6 to 5.4, and molecules of 72 to 88 kDa, focusing at pH 4.9 to 5.7, were visualized in CL and G strains, respectively. Flow cytometry and microscopic analysis revealed in both strains similar expression of MAb 3F6-reactive gp82 in live and permeabilized parasites, indicating its surface localization. The reaction of live parasites of both strains with anti-J18 antibodies was weaker than with MAb 3F6 and was increased by permeabilization. Anti-C03 antibodies bound predominantly to flagellar components in permeabilized G strain parasites, but in the CL strain the flagellum was not the preferential target for these antibodies. Host cell invasion of metacyclic forms was inhibited by J18 protein, as well as by MAb 3F6 and anti-J18 antibodies, but not by C03 protein or anti-C03 antibodies.

scholarly journals Features of host cell invasion by different infective forms of Trypanosoma cruzi

1999 ◽  
Vol 94 (suppl 1) ◽  
pp. 135-137 ◽  
Author(s):  
Renato A Mortara ◽  
Daniela O Procópio ◽  
Helena C Barros ◽  
Newton V Verbisck ◽  
Walter K Andreoli ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Invasion ◽  
Host Cell Invasion

Oral infection of mice and host cell invasion by Trypanosoma cruzi strains from Mexico

2019 ◽  
Vol 118 (5) ◽  
pp. 1493-1500 ◽  
Author(s):  
Cecilia G. Barbosa ◽  
César Gómez-Hernández ◽  
Karine Rezende-Oliveira ◽  
Marcos Vinicius Da Silva ◽  
João Paulo Ferreira Rodrigues ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Invasion ◽  
Oral Infection ◽  
Host Cell Invasion
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