scholarly journals Expression of Genes for a Flavin Adenine Dinucleotide-Binding Oxidoreductase and a Methyltransferase from Mycobacterium chlorophenolicum Is Necessary for Biosynthesis of 10-Methyl Stearic Acid from Oleic Acid in Escherichia coli

2017 ◽  
Vol 8 ◽  
Author(s):  
Shuntaro Machida ◽  
Ranjith K. Bakku ◽  
Iwane Suzuki
2012 ◽  
Vol 37 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Aleksandra Arsić ◽  
Vesna Vučić ◽  
Jasna Tepšić ◽  
Sanja Mazić ◽  
Marina Djelić ◽  
...  

The impact of chronic, intense exercise, such as in elite athletes, on phospholipids fatty acids (FA) composition has not been studied in women so far. This study aimed to investigate FA profiles in plasma and erythrocytes phospholipids in elite female water polo (N = 15) and football (N = 19) players in comparison with sedentary women. In spite of similar dietary patterns, as assessed by a food frequency questionnaire, plasma FA profile in the football players showed significantly higher proportions of stearic acid, oleic acid, and monounsaturated FA (MUFA), and significantly lower proportions of total and n-6 polyunsaturated FA (PUFA) than in the water polo and control group. The water polo players had higher percentages of palmitoleic acid and arachidonic acid than the control subjects. Erythrocyte FA profile differed among groups. We found significantly higher proportion of oleic acid and MUFA in the football group than in the controls, and decreased stearic acid and elevated palmitic and palmitoleic acid in the water polo players than in the other 2 groups. Both groups of athletes had significantly lower percentages of n-6 dihomo-γ-linolenic acid, n-6 PUFA, and total PUFA compared with the controls. The estimated activities of elongase and desaturases in erythrocytes were also altered in the athletes. Our results indicate that long-term, intense physical training significantly affects FA status of plasma and erythrocyte phospholipids in women. The observed differences between the water polo and the football players suggest that the type of regular training may contribute to the altered metabolism of FA, although possible genetic differences among the 3 study groups cannot be ruled out.


1851 ◽  
Vol 2 ◽  
pp. 134-136
Author(s):  
T. Anderson

The investigations contained in this paper were undertaken with the view of ascertaining the nature of the action of sulphur in the free state on organic substances. The author endeavoured, in the first instance, to examine the action of that agent upon some of the simpler organic compounds, but without obtaining any definite results; and finally confined his experiments entirely to the fixed oils.The distillation of oil of almonds with sulphur, which is attended by the violent evolution of sulphuretted hydrogen, afforded a peculiar nauseous oil, and a crystalline product deposited on cooling from the latter portions of the oil. In order to ascertain the source of these products, comparative experiments were made with pure stearic and oleic acids. It was thus found, that stearic acid, when distilled with sulphur, gave products identical with those obtained by its simple distillation, but that oleic acid gave an oily fluid and crystals similar to those produced from the crude oil.


2020 ◽  
Vol 8 (2) ◽  
pp. 114-119
Author(s):  
Diah Gusti Riski ◽  
Rico Gewana Resdy Maulana ◽  
Edwin Permana ◽  
Intan Lestari ◽  
Indra Lasmana Tarigan

The Shorea sumatrana (tengkawang) plant is endemic to Indonesia, especially in Kalimantan and Sumatera regions, which produces chemical diversity especially as a natural drugs. Specific aims to investigate both the profile analysis of fatty acid and antibacterial potential of tengkawang oil. The extract of tengkawang oil was carried out using the soxhlet extraction method. The profile analysis of fatty acid was verified by GC-MS and the antibacterial activity was evaluated using disc-diffusion method. The profile analysis of fatty acid of tengkawang oil indicated the presence of palmitic acid (17.26%), stearic acid (60.68%), oleic acid (11.98%), oleic acid chloride (1.80%), stearic acid chloride (1.86%), glycidyl stearate (1.92%), diethyl phthalate (4%), and 2-monopalmitin (0.5%). We determined the antibacterial activity by the diameter of inhibition of growth zone against Salmonella enteritidis, Escherichia coli, Staphylococcus aureus, and Bacillus cereus at a concentration of 12.5%, 25%, 50%. These were compared with standard tetracycline as positive control and DMSO was assigned negative control. It was found that the highest percentage of fatty acid in tengkawang oil is stearic acid, at 60.68%, and that tengkawang oil is an antibacterial agent with a concentration optimum at 25% with more susceptibility to Gram-positive than Gram-negative bacteria.


1999 ◽  
Vol 81 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Amanda E. Jones ◽  
Michael Stolinski ◽  
Ruth D. Smith ◽  
Jane L. Murphy ◽  
Stephen A. Wootton

The gastrointestinal handling and metabolic disposal of [1-13C]palmitic acid, [1-13C]stearic acid and [1-13C]oleic acid administered within a lipid–casein–glucose–sucrose emulsion were examined in normal healthy women by determining both the amount and nature of the13C label in stool and label excreted on breath as13CO2. The greatest excretion of13C label in stool was in the stearic acid trial (9.2 % of administered dose) whilst comparatively little label was observed in stool in either the palmitic acid (1.2 % of administered dose) or oleic acid (1.9 % of administered dose) trials. In both the palmitic acid and oleic acid trials, all of the label in stool was identified as being present in the form in which it was administered (i.e. [13C]palmitic acid in the palmitic acid trial and [13C]oleic acid in the oleic acid trial). In contrast, only 87 % of the label in the stool in the stearic acid trial was identified as [13C]stearic acid, the remainder was identified as [13C]palmitic acid which may reflect chain shortening of [1-13C]stearic acid within the gastrointestinal tract. Small, but statistically significant, differences were observed in the time course of recovery of13C label on breath over the initial 9 h of the study period (oleic acid = palmitic acid > stearic acid). However, when calculated over the 24 h study period, the recovery of the label as13CO2was similar in all three trials (approximately 25 % of absorbed dose). These results support the view that chain length and degree of unsaturation may influence the gastrointestinal handling and immediate metabolic disposal of these fatty acids even when presented within an emulsion.


1986 ◽  
Vol 59 (5) ◽  
pp. 800-808 ◽  
Author(s):  
James M. Sloan ◽  
Michael J. Maghochetti ◽  
Walter X. Zukas

Abstract An effort to characterize the reversion process of guayule rubber when naturally-occurring guayule resin components are present has shown that these components act as a reversion-retarding material. The amount of reversion resistance varies as a function of temperature, concentration, and type of fatty acid. Of the three fatty acids used, linoleic acid, stearic acid, and oleic acid, linoleic acid performed the best for reversion resistance, followed by stearic acid, then oleic acid. When the temperature was increased 10°C, an increase of 15% reversion was observed. This held true for the three temperatures studied. In addition, the amount of reversion improvement upon addition was 20% reversion. In the case of curing at 150°C, this resulted in 0% reversion. The 20% resistance improvment was consistent for the 3 temperatures studied.


2013 ◽  
Vol 469 ◽  
pp. 171-174 ◽  
Author(s):  
Ning Zhang ◽  
Si Yao Sui ◽  
Zhe Wang ◽  
Zhong Su Ma

Edible films were prepared using soy protein isolate (4g/100g), oleic acid (0-2g/100g) and stearic acid (0-2g/100g). Effects of the type and ratio of fatty acids (oleic acid and stearic acid) on the thermal properties of soybean protein isolate-based films were investigated. The results indicated that the addition of oleic acid and stearic acid take a significant effect on the thermal stability of soybean protein isolate-based films, as may attribute to that oleic acid is an amphiphilic substance that interacts with both polar and hydrophobic sites on proteins, thus it could improve the functional properties of the films. Besides, the solid state and hydrophobic nature of stearic acid could help limit water diffusion in the matrix more efficiently when it is well-integrated in the matrix through the surfactant action of oleic acid.


FEBS Letters ◽  
1985 ◽  
Vol 192 (2) ◽  
pp. 283-288 ◽  
Author(s):  
Alejandro M. Viale ◽  
Hirokazu Kobayashi ◽  
Tetsuko Takabe ◽  
Takashi Akazawa

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (04) ◽  
pp. 39-42
Author(s):  
M. S Kale ◽  
◽  
K. S. Laddha

Fixed oil obtained from the seeds of Momordica tuberosa (Roxb) Cogn. fruits (family: Cucurbitaceae) was analyzed using GC-MS. Five compounds, namely palmitic acid (hexadecanoic acid), oleic acid (9-octadecenoic acid), stearic acid (octadecanoic acid), ?-eleostearic acid (9, 11, 13-octadecatrienoic acid) and gama-linolenic acid (6, 9, 12-octadecatrienoic acid) were found to be major compounds in fixed oil obtained from the seeds of M. tuberosa fruits. Physical constants saponification value, unsaponifiable matter and acid value were found to be 182.4, 1.56% w/w and 11.44 respectively.


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