scholarly journals Detection of Inter-Lineage Natural Recombination in Avian Paramyxovirus Serotype 1 Using Simplified Deep Sequencing Platform

2016 ◽  
Vol 7 ◽  
Author(s):  
Dilan A. Satharasinghe ◽  
Kavitha Murulitharan ◽  
Sheau W. Tan ◽  
Swee K. Yeap ◽  
Muhammad Munir ◽  
...  
Author(s):  
Fedri Rell ◽  
Anak Agung Ayu Mirah Adi ◽  
I Gusti Ngurah Kade Mahardika

Newcastle disease (ND) merupakan penyakit kontagius yang disebabkan virus Avian paramyxovirus serotype 1 yang menginfeksi bangsa unggas. Penelitian ini bertujuan untuk menganalisis pohon filogeni berdasarkan sekuan gen daerah pemotonan protein fusion dari virus ND pada peternakan ayam di provinsi Bali dari tahun 2013 sampai 2014. Sebanyak empat isolat virus dari kasus ayam sakit/mati yang dicurigai terinfeksi oleh virus Newcastle disease. Sekuen potongan gen F disejajarkan dan dianalisis dengan program MEGA5. Analisis sekuen asam amino daerah pemotongan protein F keempat isolat memiliki sekuen 112R-R-Q-K-R-F117 dan dilanjutkan dengan analisis pohom filogeni yang menunjukan bahwa keempat isolat merupakan virus Newcastle disease yang virulen. Penelitian ini menunjukan bahwa keempat isolat lapang Bali tahun 2013 sampai 2014 masuk ke dalam kelompok virus Newcastle disease genotipe VII.


2012 ◽  
Vol 2012 ◽  
pp. 1-17 ◽  
Author(s):  
Nichole L. Hines ◽  
Cathy L. Miller

Avian paramyxovirus serotype-1 (APMV-1) is capable of infecting a wide range of avian species leading to a broad range of clinical symptoms. Ease of transmission has allowed the virus to spread worldwide with varying degrees of virulence depending on the virus strain and host species. Classification systems have been designed to group isolates based on their genetic composition. The genetic composition of the fusion gene cleavage site plays an important role in virulence. Presence of multiple basic amino acids at the cleavage site allows enzymatic cleavage of the fusion protein enabling virulent viruses to spread systemically. Diagnostic tests, including virus isolation, real-time reverse-transcription PCR, and sequencing, are used to characterize the virus and identify virulent strains. Genetic diversity within APMV-1 demonstrates the need for continual monitoring for changes that may arise requiring modifications to the molecular assays to maintain their usefulness for diagnostic testing.


1992 ◽  
Vol 39 (1-10) ◽  
pp. 153-158 ◽  
Author(s):  
U. Wernery ◽  
J. D. Remple ◽  
U. Neumann ◽  
D. J. Alexander ◽  
Ruth J. Manvell ◽  
...  

2013 ◽  
Vol 17 ◽  
pp. 260-268 ◽  
Author(s):  
Yee Ling Chong ◽  
Tommy Tsan-Yuk Lam ◽  
Oekyung Kim ◽  
Huaguang Lu ◽  
Patty Dunn ◽  
...  

2016 ◽  
Vol 52 (3) ◽  
pp. 657 ◽  
Author(s):  
Kerri Pedersen ◽  
David R. Marks ◽  
Claudio L. Afonso ◽  
Scott R. Stopak ◽  
Dawn Williams-Coplin ◽  
...  

2012 ◽  
Vol 7 (2) ◽  
pp. e17-e18
Author(s):  
Nichole L. Hines ◽  
Mary Lea Killian ◽  
Janice C. Pedersen ◽  
Monica M. Reising ◽  
Nestor A. Mosos ◽  
...  

2016 ◽  
Vol 11 (2) ◽  
pp. 50-58
Author(s):  
A. V. Glushchenko ◽  
K. S. Yurchenko ◽  
A. K. Yurlov ◽  
Yu. G. Yushkov ◽  
M. Yu. Shchelkanov ◽  
...  

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