scholarly journals Aflatoxin Effects in White Leghorn Chickens Selected for Response to Sheep Erythrocyte Antigen

1985 ◽  
Vol 64 (6) ◽  
pp. 1065-1070 ◽  
Author(s):  
C.O. UBOSI ◽  
P.B. HAMILTON ◽  
E.A. DUNNINGTON ◽  
P.B. SIEGEL
Keyword(s):  
Sheep Erythrocyte ◽  
White Leghorn ◽  
Erythrocyte Antigen

scholarly journals Aflatoxin Effects in White Leghorn Chicken s Selected for Response to Sheep Erythrocyte Antigen

1985 ◽  
Vol 64 (6) ◽  
pp. 1071-1076 ◽  
Author(s):  
C.O. UBOSI ◽  
W.B. GROSS ◽  
P.B. HAMILTON ◽  
M. EHRICH ◽  
P.B. SIEGEL
Keyword(s):  
Sheep Erythrocyte ◽  
White Leghorn ◽  
White Leghorn Chicken ◽  
Erythrocyte Antigen

Immunosuppression by Murine Sarcoma Virus (Moloney)

1970 ◽  
Vol 1 (3) ◽  
pp. 288-292
Author(s):  
S. P. Chan ◽  
W. A. Hook ◽  
W. Turner ◽  
M. A. Chirigos
Keyword(s):  
Antibody Response ◽  
Survival Time ◽  
Primary Tumor ◽  
Sheep Erythrocyte ◽  
Humoral Antibody ◽  
Secondary Tumors ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Erythrocyte Antigen ◽  
Murine Sarcoma

Infection of mice with the murine sarcoma virus (Moloney) markedly suppressed the humoral antibody response to sheep erythrocyte antigen injected 10 days after infection, when tumor size was maximal, and on day 26, when primary tumors had partially regressed. Humoral antibody response was also inhibited when antigen was injected at the time secondary tumors and metastases were evident. No significant suppression of humoral antibody was seen when mice were injected with sheep erythrocyte antigen 5 days after virus infection. Inhibition of the cellular immune response of murine sarcoma virus (Moloney)-infected mice, as measured by the increased survival time of skin grafts, was also determined. Mice that were infected 5 days prior to grafting demonstrated prolonged survival of grafts, suggesting a suppression of cellular immunity. These mice had a graft survival time 14 days greater than noninfected controls. No significant prolongation of graft survival was seen in mice grafted at the times of maximum primary tumor growth, of primary tumor regression, or when secondary tumors had appeared.

scholarly journals A B-lymphoma cell line that forms rosettes with neuraminidase-treated sheep erythrocytes through monoclonal surface immunoglobulin

Blood ◽  
1982 ◽  
Vol 59 (6) ◽  
pp. 1220-1224
Author(s):  
Y Tsutsumi ◽  
S Suzuki ◽  
A Mikata ◽  
H Suzuki ◽  
K Kageyama ◽  
...  
Keyword(s):  
Gradient Centrifugation ◽  
Sheep Erythrocyte ◽  
Percoll Gradient ◽  
Neuraminidase Treatment ◽  
B Lymphoma ◽  
Sheep Erythrocytes ◽  
Surface Immunoglobulin ◽  
B Lymphoma Cells ◽  
Kappa Chain ◽  
Erythrocyte Antigen

Undifferentiated lymphoma from a 39-year-old female became serially xenotransplantable to preirradiated nude mice. The tumor cells (KT) possessed a monoclonal surface immunoglobulin (SIg mu, kappa) and formed rosettes with neuraminidase-treated sheep erythrocytes (SEn). Precise characterizations of the SEn rosette, however, revealed the following facts: (1) Neuraminidase-untreated or 2- aminoethylisothiuronium bromide (AET) treated sheep erythrocytes were not bound to the KT cells. (2) SEn rosettes on the KT cells did not show a temperature dependency. (3) Neuraminidase-treated erythrocytes from man, horse, mouse, and rabbit were not bound to the KT cells. (4) Preincubation of the KT cells with antipolyvalent immunoglobulin or anti-kappa-chain serum abolished the SEn rosette formation. (5) Trypsinization decreased both SEn rosettes and SIg on the KT cells. (6) SEn rosettes on the KT cells were too loose to be separated from nonrosetting cells by a Percoll gradient centrifugation method. Summarizing these results, the monoclonal SIg on the KT cells recognized sheep erythrocyte antigen(s) that were exposed only after the neuraminidase treatment. Therefore, this was considered to be a case with peculiar B-lymphoma cells that bound SEn through their SIg.

scholarly journals MATURATION OF HEMOLYSIN-PRODUCING CELL CLONES

1969 ◽  
Vol 130 (3) ◽  
pp. 543-556 ◽  
Author(s):  
George C. Saunders
Keyword(s):  
Cell Division ◽  
Latent Period ◽  
Induction Period ◽  
Generation Time ◽  
Sheep Erythrocyte ◽  
Precursor Cells ◽  
Cell Clones ◽  
Synchronous Cell ◽  
Erythrocyte Antigen

Investigations of the induction period of an in vitro hemolysin response to sheep erythrocyte antigen revealed the following: 1. After antigen stimulation precursors of plaque-forming cells rapidly maturate to the point of hemolysin production. 2. Initial maturation probably occurs in the absence of cell division. 3. After initial maturation, a latent period of about 12 hr occurs before the first doubling of PFC. 4. At least the first three cell doublings are synchronous, with a generation time of 7–8 hr. 5. Synchronous cell division implies that all precursor cells are at the same point in the cell cyde when they are initially stimulated.

scholarly journals A B-lymphoma cell line that forms rosettes with neuraminidase-treated sheep erythrocytes through monoclonal surface immunoglobulin

Blood ◽  
1982 ◽  
Vol 59 (6) ◽  
pp. 1220-1224 ◽  
Author(s):  
Y Tsutsumi ◽  
S Suzuki ◽  
A Mikata ◽  
H Suzuki ◽  
K Kageyama ◽  
...  
Keyword(s):  
Gradient Centrifugation ◽  
Sheep Erythrocyte ◽  
Percoll Gradient ◽  
Neuraminidase Treatment ◽  
B Lymphoma ◽  
Sheep Erythrocytes ◽  
Surface Immunoglobulin ◽  
B Lymphoma Cells ◽  
Kappa Chain ◽  
Erythrocyte Antigen

Abstract Undifferentiated lymphoma from a 39-year-old female became serially xenotransplantable to preirradiated nude mice. The tumor cells (KT) possessed a monoclonal surface immunoglobulin (SIg mu, kappa) and formed rosettes with neuraminidase-treated sheep erythrocytes (SEn). Precise characterizations of the SEn rosette, however, revealed the following facts: (1) Neuraminidase-untreated or 2- aminoethylisothiuronium bromide (AET) treated sheep erythrocytes were not bound to the KT cells. (2) SEn rosettes on the KT cells did not show a temperature dependency. (3) Neuraminidase-treated erythrocytes from man, horse, mouse, and rabbit were not bound to the KT cells. (4) Preincubation of the KT cells with antipolyvalent immunoglobulin or anti-kappa-chain serum abolished the SEn rosette formation. (5) Trypsinization decreased both SEn rosettes and SIg on the KT cells. (6) SEn rosettes on the KT cells were too loose to be separated from nonrosetting cells by a Percoll gradient centrifugation method. Summarizing these results, the monoclonal SIg on the KT cells recognized sheep erythrocyte antigen(s) that were exposed only after the neuraminidase treatment. Therefore, this was considered to be a case with peculiar B-lymphoma cells that bound SEn through their SIg.

Dexamethasone Effect on Embryonic Chick Respiratory Epithelium

1982 ◽  
Vol 40 ◽  
pp. 248-249
Author(s):  
M.R. Richter ◽  
R.V. Blystone
Keyword(s):  
Lung Development ◽  
Critical Role ◽  
Respiratory Epithelium ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
White Leghorn ◽  
Lung Maturation ◽  
Synthetic Analogs ◽  
Lung Physiology ◽  
Avian Lung

Dexamethasone and other synthetic analogs of corticosteroids have been employed clinically as enhancers of lung development. The mechanism(s) by which this steroid induction of later lung maturation operates is not clear. This study reports the effect on lung epithelia of dexamethasone administered at different intervals during development. White Leghorn chick embryos were used so as to remove possible maternal and placental influences on the exogenously applied steroid. Avian lung architecture does vary from mammals; however, respiratory surfactant produced by the lung epithelia serves an equally critical role in avian lung physiology.

Improved preservation of the otolithic membranes

1987 ◽  
Vol 45 ◽  
pp. 914-915
Author(s):  
G. M. Cohen ◽  
J. S. Grasso ◽  
M. L. Domeier ◽  
P. T. Mangonon
Keyword(s):  
Toluidine Blue ◽  
Potassium Phosphate ◽  
Vestibular Function ◽  
Spatial Relations ◽  
White Leghorn ◽  
Supporting Cells ◽  
Semithin Sections ◽  
Micromechanical Models ◽  
Soluble Components

Any explanation of vestibular micromechanics must include the roles of the otolithic and cupular membranes. However, micromechanical models of vestibular function have been hampered by unresolved questions about the microarchitectures of these membranes and their connections to stereocilia and supporting cells. Otolithic membranes are notoriously difficult to preserve because of severe shrinkage and loss of soluble components. We have empirically developed fixation procedures that reduce shrinkage artifacts and more accurately depict the spatial relations between the otolithic membranes and the ciliary bundles and supporting cells.We used White Leghorn chicks, ranging in age from newly hatched to one week. The inner ears were fixed for 3-24 h in 1.5-1.75% glutaraldehyde in 150 mM KCl, buffered with potassium phosphate, pH 7.3; when postfixed, it was for 30 min in 1% OsO4 alone or mixed with 1% K4Fe(CN)6. The otolithic organs (saccule, utricle, lagenar macula) were embedded in Araldite 502. Semithin sections (1 μ) were stained with toluidine blue.

Effect of Supplementation of Multi Enzymes on Production Performance and Egg Quality Traits in White Leghorn Layers

2016 ◽  
Vol 12 (2) ◽  
Author(s):  
C. Pandian ◽  
A. Sundaresan ◽  
A. V. Omprakash
Keyword(s):  
Egg Production ◽  
Egg Quality ◽  
Live Weight ◽  
Economic Benefits ◽  
Production Performance ◽  
Feed Consumption ◽  
Quality Traits ◽  
White Leghorn ◽  
Significant Difference ◽  
Enzyme Supplementation

The present study was conducted to assess the effect of supplementation of Multi-enzymes with lysophospholipids on production performance of pure line White Leghorn layers. Body weights before and after the experiment did not differ significantly across the experimental diets. Irrespective of the dietary treatments, the birds gained 3.83 per cent of live weight relative to its initial body weight. Mean per cent Hen housed egg production was significantly (Pis less than 0.05) higher in diet supplemented with 0.10 MEC-L than other groups. Mean egg weight and average daily feed consumption during 25 to 35 weeks of age indicated no significant effect of enzyme supplementation. Average daily feed consumption per bird in control, 0.05 % and 0.1% multi-enzyme supplemented groups was 108.13, 105.66 and 107.67 g respectively and birds offered control diet recorded numerically more feed intake than enzyme supplemented groups. Comparatively low feed per egg was observed in 0.10 per cent group followed by 0.05 per cent group which offers economic benefits than control diets. However, the egg quality traits between different dietary enzyme supplementation groups showed no significant difference.

Comparative Histomorphochemical Studies on Uropygial Gland of Kadaknath and White Leghorn Breeds of Poultry

2017 ◽  
Vol 13 (02) ◽  
Author(s):  
S. Kanasiya ◽  
S. K. Karmore ◽  
S. K. Deshmukh ◽  
R. K. Barhaiya ◽  
S. K. Gupta
Keyword(s):  
Characteristic Feature ◽  
Periodic Acid ◽  
Elastic Fibers ◽  
Moderate Activity ◽  
Uropygial Gland ◽  
White Leghorn ◽  
Preen Gland ◽  
Central Cavity ◽  
Tissue Samples ◽  
Melanin Pigmentation

The present study was conducted on ten each uropygial glands of 6 to 8 months old healthy Kadaknath and White Leghorn breeds of poultry. The tissue samples were stained by H and E for normal histological structures, Verhoeff’s stain for collagen and elastic fibers, PAS (Periodic Acid Schiff’s) for glycogen and Alcian Blue PAS method for acid mucopolysaccharides. The uropygial gland in Kadaknath and White Leghorn was composed of two lobes. Each lobe had a single duct and these ducts were joined together by isthmus. The thickness of capsule was more in White Leghorn than Kadaknath breed. The lumen of tubules showed higher concentration of secretary product in Kadaknath breed. Numbers of tubules were higher in Kadaknath. No aggregation of lymphocytes was found in the preen gland of White Leghorn, whereas in Kadaknath, there was large number of lymphocytes aggregation alongwith lymphatic nodules. Melanin pigmentation was the characteristic feature of Kadaknath which was found towards the central cavity and in between ductules. The capsule of White Leghorn showed intense PAS activity, while moderate activity was found in Kadaknath breed of poultry. Intense ACPase reaction was noticed in capsule of uropygial gland of Kadaknath and White Leghorn breeds of poultry.

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