scholarly journals Comparative analysis of tryptophan oxygenase activity and glucocorticoid receptor under the influence of insulin

2008 ◽  
pp. 101-107
Author(s):  
ER Isenović ◽  
Z Zakula ◽  
G Koricanac ◽  
NR Stepić
Keyword(s):  
Glucocorticoid Receptor ◽  
Wistar Rats ◽  
Receptor Complex ◽  
Complex Stability ◽  
Cell Nuclei ◽  
Tryptophan Oxygenase ◽  
Oxygenase Activity ◽  
Male Wistar Rats ◽  
Hepatic Regulation ◽  
Different Doses

This investigation addresses the interaction of insulin (INS) and glucocorticoid (GC) signaling in the hepatic regulation of tryptophan oxygenase (TO) enzyme activity in the rat. Male Wistar rats (200-250 g b.w) received an injection of the different doses of INS (10, 25, 50, 70 and 100 μg/200 g b.w., i.p.) and were used for experiments 3 h and 18 h after INS administration. This study shows that maximum of TO activity was found at dose of 50 μg of INS with peak increases observed at 3 h and 18 h after injection of INS, while INS had no effect on TO activity in adrenalectomized rats. The analysis of INS effects on glucocorticoid receptor-complex (GC/GR complex) stability shows that complexes from INS-treated rats are less stable than those from control animals. In addition, INS-stimulated stability of glucocorticoid receptor (GR) protein was significantly increased from the controls. Furthermore, the results show that GC/GR complexes from INS-treated rats could be activated and accumulated at higher rate in cell nuclei of control animals. These data support the involvement of INS in modulation of GC signaling pathway which mediates, in part, the activity of TO.

scholarly journals Effect of acute adrenalectomy on rat liver glucocorticoid receptor

2006 ◽  
Vol 58 (3) ◽  
pp. 153-159 ◽  
Author(s):  
Esma Isenovic ◽  
Marija Radojcic ◽  
Zorica Zakula ◽  
Goran Koricanac ◽  
Nevena Ribarac-Stepic
Keyword(s):  
Glucocorticoid Receptor ◽  
Protein Content ◽  
Binding Sites ◽  
Wistar Rats ◽  
Cellular Distribution ◽  
Receptor Complex ◽  
Glucocorticoid Hormones ◽  
Structural And Functional Properties ◽  
Male Wistar Rats ◽  
Molecular Aspects

In order to improve current clinical treatment of human hypocortisolism, it is necessary to understand molecular aspects of this pathophysiology. In this study liver tissues from male Wistar rats were used as an experimental model to study structural and functional properties of glucocorticoid receptor (GR) in the absence of glucocorticoid hormones (GC). Results show that acute adrenalectomy (ADX) significantly increases the number of GR binding sites and GR protein content. In addition, acute ADX stimulates increase in stability of the GR, decrease in stability of the glucocorticoid- receptor complex (G-R), and changes in accumulation of the G-R complex in nuclei and its cellular distribution. .

Effect of different doses of 2,4-dichlorophenoxyacetic acid (2,4-d) on cardiac parameters in male Wistar rats

2020 ◽  
Vol 28 (3) ◽  
pp. 3078-3087
Author(s):  
Raíssa De Oliveira Mantovani ◽  
Dyovana Gomes Pinheiro ◽  
Geovana Letícia Fernandes De Oliveira ◽  
Stéfani Nobrega Perrud ◽  
Giovana Rampazzo Teixeira ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Dichlorophenoxyacetic Acid ◽  
Male Wistar Rats ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Different Doses

Nephrotoxicity as a cause of death in male wistar rats exposed to toxic doses of paracetamol\methionine combination

2011 ◽  
Vol 1 (4) ◽  
pp. 182-187
Author(s):  
A. A. Iyanda ◽  
J. I. Anetor ◽  
F. A. Adeniyi ◽  
C. I. Iheakanwa
Keyword(s):  
Uric Acid ◽  
Cause Of Death ◽  
Wistar Rats ◽  
Hepatic Necrosis ◽  
Renal Abnormality ◽  
Renal Histology ◽  
Male Wistar Rats ◽  
Physiologic Saline ◽  
Different Doses

In an earlier study, we observed that male Wistar rats administered withtoxic doses of methionine containing paracetamol formulation(acetaminophen) did not manifest hepatic necrosis even at doses as high as3000 mg\kg and 5000 mg\kg body weight (BW) yet death occurred. Thisstudy sets out to investigate the cause of death by focusing on another sensitiveorgan to acetaminophen exposure and to highlight the role of some vitaminsin this. Thirty male Wistar rats were divided into six groups consisting 5rats in each, and further administered with different doses of paracetamol\methionine, ranging from 100 mg\kg – 5000 mg\kg. 5 rats, suppliedwith only physiologic saline were considered as control. Results show thatrats exposed to 100mg\kg, 350 mg\kg and 1000 mg\kg BW did not exhibitany form of renal abnormality. The nephrotoxic indices consisting of urea,creatinine and uric acid were not significantly increased in comparison tocontrol (p>0.05). Renal histology was also not identified as abnormal; moreover0% mortality was recorded for these groups. However, the creatininewas significantly increased in 3000 mg\kg group (p

Gastroprotective activity of essential oils from turmeric and ginger

2015 ◽  
Vol 26 (1) ◽  
Author(s):  
Vijayasteltar B. Liju ◽  
Kottarapat Jeena ◽  
Ramadasan Kuttan
Keyword(s):  
Gastric Ulcer ◽  
Antioxidant Enzymes ◽  
Essential Oils ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Degree Of Protection ◽  
Male Wistar Rats ◽  
Different Doses

AbstractTurmeric (Turmeric and ginger were evaluated for their antiulcer activity against ethanol-induced ulcers in male Wistar rats at different doses: 100, 500 and 1000 mg/kg body weight. Ethanol was used to induce gastric ulcer in Wistar rats. Parameters such as ulcer index, histopathology and levels of antioxidant enzymes such as glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase and glutathione (GSH) levels were measured to assess the degree of protection produced by the essential oils.TEO and GEO inhibited ulcer by 84.7% and 85.1%, respectively, as seen from the ulcer index. Reduced antioxidant enzymes such as GPx, SOD, catalase and GSH produced by alcohol administration were significantly (p<0.001) increased by simultaneous administration of TEO and GEO. Histopathological examination showed that ethanol-induced lesions such as necrosis, erosion and hemorrhage of the stomach wall were significantly reduced after oral administration of essential oils.Results suggest that TEO and GEO could reduce the gastric ulcer in rat stomach as seen from the ulcer index and histopathology of the stomach. Moreover, oxidative stress produced by ethanol was found to be significantly reduced by TEO and GEO.

scholarly journals Prolonged use of the food dye tartrazine (FD&C yellow n° 5) and its effects on the gastric mucosa of Wistar rats

2007 ◽  
Vol 67 (1) ◽  
pp. 141-145 ◽  
Author(s):  
ILD. Moutinho ◽  
LC. Bertges ◽  
RVC. Assis
Keyword(s):  
Drinking Water ◽  
Wistar Rats ◽  
Sulfanilic Acid ◽  
Control Group ◽  
Allergic Reactions ◽  
Food Dye ◽  
Male Wistar Rats ◽  
Group A ◽  
Ad Libitum ◽  
Different Doses

Tartrazine is one of the most widely used artificial foods, drugs and cosmetic dyes. It is a nitrous derivative and is known to cause allergic reactions such as asthma and urticaria, as well as having been the focus of studies on mutagenesis and carcinogenesis due to its transformation into aromatic amine sulfanilic acid after being metabolized by the gastrointestinal microflora. 45 male Wistar rats were assigned to a control group (A) or a treatment one (B). The treatment group received 7.5 mg.kg-1.day-1 of tartrazine daily in drinking water offered ad libitum for ten months from weaning to the age of twelve months. There was a significant increase in the number of lymphocytes and eosinophils of the gastric antrum mucosa. No carcinogenetic changes in any gastric area were observed during the study. As tartrazine belongs to the azo class, it is still a possible food carcinogen. Other studies with different doses and schedules, observing their effects associated to other carcinogens should be carried out if their safe use is to be recommended.

Evaluation of anti-inflammatory and analgesic activities and the phytochemical study of Astragalus arbusculinus gum in animal models

2015 ◽  
Vol 26 (4) ◽  
Author(s):  
Asie Shojaii ◽  
Manijeh Motevalian ◽  
Nazanin Rahnama
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Inflammatory Diseases ◽  
Phytochemical Study ◽  
Analgesic Effects ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
First Time ◽  
Different Doses

AbstractThe importance of inflammatory diseases and side effects of conventional drugs necessitate the finding of new anti-inflammatory agents from natural sources. In this study, for the first time, the anti-inflammatory and analgesic effects of the aqueous extract ofThirty-five male Wistar rats were divided into five groups and pretreated with different doses ofThe extract ofThe aqueous extract of

scholarly journals The Effects of Hydroalcoholic Extract of Satureja khuzestanica on Naloxone-Induced Morphine Withdrawal Symptoms in Wistar Rats

2020 ◽  
Vol 5 (1) ◽  
pp. 16-21
Author(s):  
Bahareh Ebrahimi ◽  
Saeed Esmaeili-Mahani
Keyword(s):  
Weight Loss ◽  
Opioid Dependence ◽  
Wistar Rats ◽  
Morphine Withdrawal ◽  
Withdrawal Symptoms ◽  
Control Group ◽  
Analgesic Tolerance ◽  
Male Wistar Rats ◽  
Satureja Khuzestanica ◽  
Different Doses

Introduction: The inflammatory system, oxidative stress, and cholinergic pathways are some important factors in long-term opioid dependence withdrawal. The adverse effects of some adjunctive medications on withdrawal symptoms treatment limit their clinical efficiency and finding compounds with inhibitory effects on opioid dependence can be helpful. The antioxidant, antinociceptive, and anti-inflammatory properties of Satureja khuzestanica extract (SKE) have been noted. Additionally, it could reduce morphine analgesic tolerance. Given that no study has assessed the effect of SKE on morphine-induced withdrawal symptoms, we have tried to investigate it in this study. Methods: Male Wistar rats were used in this study. The rats were treated with morphine for 7 days. The control group received saline, and intervention groups received SKE intragastrically by gavage (100 mg/kg, 50 mg/kg, and 25 mg/kg) 15 minutes before morphine injections. Five hours after the last injection, naloxone was used and withdrawal symptoms were assessed for one hour. SPSS software version 16.0 was used for statistical analysis, and P≤0.05 was considered statistically significant. Results: SKE diminished weight loss and jumping (P≤0.001). It decreased grooming behaviors and cramps (P≤0.01). SKE was found to decrease morphine withdrawal symptoms and 100 mg/kg was the most effective dose. Additionally, different doses of SKE were able to eliminate ptosis, diarrhea, and teeth chattering in animals; however, different doses had different effects on withdrawal symptoms. Conclusion: Naloxone significantly increased the frequency of jumping, cramps, weight loss, grooming, and induced ptosis, diarrhea, and teeth chattering. Treatment with SKE can significantly reduce morphine withdrawal symptoms. This can be done through its ability to decrease inflammation.

scholarly journals The effects of chronic manganese administration on blood pressure in rats

2014 ◽  
Vol 66 (1) ◽  
pp. 157-160 ◽  
Author(s):  
Serban Lacramioara ◽  
Mihai Hogas ◽  
Alin Ciobica ◽  
Romeo Dobrin ◽  
Florin Petrariu
Keyword(s):  
Blood Pressure ◽  
Repeated Measures ◽  
Wistar Rats ◽  
Vascular System ◽  
Time Effect ◽  
Pressure Measurements ◽  
Disease Mechanisms ◽  
Male Wistar Rats ◽  
Font Color ◽  
Different Doses

Several trace elements, including manganese (Mn), affect the cardiovascular system and are implicated in some cardiovascular disease mechanisms. The effects of Mn on the vascular system, such as in the control of blood flow and blood pressure, are not completely understood. Thus, the main objective of the present study was to determine the effects of a 45-day exposure to two different doses of Mn on blood pressure values of male Wistar rats. Our results showed a significant time effect (p<0.001, ANOVA, repeated measures) on the values of blood pressure measurements during 45 days of Mn treatment of rats with two doses (3 mg/kg/day and 10 mg/kg/day). Additionally, we observed significant differences in the values of blood pressure measurements, especially on days 2 (p<0.001), 9 (p<0.05), 24 (p<0.05), 28 (p<0.01) and 43 (p<0.05). Further studies are necessary in order to establish the mechanism and relevance of Mn in this area of research. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS161021104E">10.2298/ABS161021104E</a><u></b></font>

Sign in / Sign up

Export Citation Format

Share Document