scholarly journals Optimization of Culture Conditions for Exopolysaccharide Production by a Probiotic Strain of Lactobacillus rhamnosus E/N

2014 ◽  
Vol 63 (2) ◽  
pp. 253-257 ◽  
Author(s):  
MAGDALENA POLAK-BERECKA ◽  
ADAM WAŚKO ◽  
AGNIESZKA KUBIK-KOMAR
Keyword(s):  
Batch Fermentation ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Culture Conditions ◽  
Optimal Conditions ◽  
Working Volume ◽  
Burman Design ◽  
Specific Culture ◽  
Plackett Burman Design ◽  
Mrs Medium

The effects of culture conditions on exopolysaccharides (EPS) production by a probiotic Lb. rhamnosus E/N have been studied using the Plackett-Burman design. Process optimization was performed in stationary cultures to maximize the production of EPS. In order to verify the optimal conditions, an analysis was performed of EPS production in fermentation culture. Batch fermentation was carried out at working volume of 2.51. The optimal temperature, pH, carbon source, and nitrogen source conditions were 37 degrees C, pH 5.0, galactose, and yeast extract, respectively. EPS production was improved by 210.28 mg/l in stationary cultures compared to 134.2 mg/l in a control grown on commercial MRS medium. The fermentor experiment showed the possibility of increasing EPS biosynthesis by 175.8%. Our results clearly demonstrate that in the case of Lb. rhamnosus E/N specific culture conditions can enhance EPS production for possible application in the industry.

scholarly journals Optimization of Large-Scale Culture Conditions for the Production of Cordycepin withCordyceps militarisby Liquid Static Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Chao Kang ◽  
Ting-Chi Wen ◽  
Ji-Chuan Kang ◽  
Ze-Bing Meng ◽  
Guang-Rong Li ◽  
...  
Keyword(s):  
Large Scale ◽  
Total Content ◽  
Culture Conditions ◽  
Key Factors ◽  
Working Volume ◽  
Large Scale Culture ◽  
Factor Design ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

Cordycepin is one of the most important bioactive compounds produced by species ofCordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.

Fermentation Conditions Optimization of Anti-Tumor Active Metabolites from Marine Penicillium sp. HGQ6

2011 ◽  
Vol 340 ◽  
pp. 344-350
Author(s):  
Guo Lei ◽  
Wen Cheng Zhu ◽  
Chao Liu
Keyword(s):  
Optimal Conditions ◽  
Inhibition Activity ◽  
Active Metabolites ◽  
Fermentation Conditions ◽  
Burman Design ◽  
Predicted Values ◽  
Initial Medium ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design ◽  
Active Substances

The objectives of this study were to optimize fermentation conditons of anti-tumor active metabolites from marine penicillium sp. HGQ6. Based on the inhibition activity against stomach cancer cell BGC823, firstly, the initial medium was screened, then seven factors including peptone, sucrose, K2HPO4, FeSO4, pH, temperature and agitation were investigated by Plackett-Burman design, and the important variables including pH, temperature and K2HPO4 were optimized by using Response surface methodology (RSM). Experimental results showed that the optimum fermentation conditions were peptone 1%, sucrose 3%, K2HPO4 0.06%, FeSO4 0.001%, pH6.41, temperature 35°C and agitation 180 r·min-1. Under these optimal conditions, the actual inhibition ratio achieved 90.4%, which agreed with the predicted values. This indicated the good feasibility of Plackett-Burman design and RSM in the optimization of fermentation conditions for production of anti-tumor active substances from stain HGQ6.

scholarly journals Medium components screening for l (+) - lactic acid production by lactobacillus rhamnosus atcc 9595 using a plackett-burman design

2019 ◽  
Vol 5 (10) ◽  
pp. 22271-22285
Author(s):  
Marília Crivelari Cunha ◽  
Michelle Thiemi Masotti ◽  
Olga Lucía Mondragón-Bernal ◽  
José Guilherme Lembi Ferreira Alves
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Lactobacillus Rhamnosus ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design

scholarly journals BNC Biosynthesis with Increased Productivity in a Newly Designed Surface Air-Flow Bioreactor

2020 ◽  
Vol 10 (11) ◽  
pp. 3850
Author(s):  
Izabela Cielecka ◽  
Małgorzata Ryngajłło ◽  
Stanisław Bielecki
Keyword(s):  
Mechanical Properties ◽  
Air Flow ◽  
Culture Conditions ◽  
Production Costs ◽  
High Yield ◽  
Flow Ratio ◽  
Culture Time ◽  
Wide Range ◽  
Burman Design ◽  
Plackett Burman Design

The application of bacterial cellulose (BNC) could be widely expanded if the production costs were reduced. This study aims to determine factors simultaneously affecting the yield and tensile strength of BNC in a newly designed surface air-flow bioreactor (SAF). For this purpose, a two-stage study was done. Firstly, the most important factors for high yield were determined based on the Plackett–Burman Design. Secondly, impact of the chosen variables on both responses was assessed in a wide range of factor values. The greatest influence on the yield and mechanical strength was proved for such factors as air-flow ratio, glucose concentration, and culture time. The productivity in a SAF bioreactor with controlled air-flow ratio was enhanced by 65%. In terms of mechanical properties, the stress of BNC membranes varied from 0.8 to 6.39 MPa depending on the culture conditions. The results of the performed tests make a useful basis for future optimizations.

scholarly journals Optimization of Acid Protease Production byAspergillus nigerI1 on Shrimp Peptone Using Statistical Experimental Design

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Rayda Siala ◽  
Fakher Frikha ◽  
Samiha Mhamdi ◽  
Moncef Nasri ◽  
Alya Sellami Kamoun
Keyword(s):  
Culture Conditions ◽  
Medium Composition ◽  
Acid Protease ◽  
Protease Production ◽  
Statistical Experimental Design ◽  
Initial Ph ◽  
Burman Design ◽  
The Cost ◽  
Optimized Conditions ◽  
Plackett Burman Design

Medium composition and culture conditions for the acid protease production byAspergillus nigerI1 were optimized by response surface methodology (RSM). A significant influence of temperature, KH2PO4, and initial pH on the protease production was evaluated by Plackett-Burman design (PBD). These factors were further optimized using Box-Behnken design and RSM. Under the proposed optimized conditions, the experimental protease production (183.13 U mL−1) closely matched the yield predicted by the statistical model (172.57 U mL−1) withR2=0.914. Compared with the initial M1 medium on which protease production was 43.13 U mL−1, a successful and significant improvement by 4.25 folds was achieved in the optimized medium containing (g/L): hulled grain of wheat (HGW) 5.0; KH2PO41.0; NaCl 0.3; MgSO4(7H2O) 0.5; CaCl2(7H2O) 0.4; ZnSO40.1; Na2HPO41.6; shrimp peptone (SP) 1.0. The pH was adjusted at 5 and the temperature at30°C. More interestingly, the optimization was accomplished using two cheap and local fermentation substrates, HGW and SP, which may result in a significant reduction in the cost of medium constituents.

Plackett-Burman Design for Screening Culture Conditions in Cellulase Production by Penicillium decumbens and Enzyme Characterization

2012 ◽  
Vol 518-523 ◽  
pp. 5578-5585
Author(s):  
Nan Ban ◽  
Yu Jie Zhou ◽  
Yan Ping Ye ◽  
Lin Mei Dai ◽  
Alatangaole Damirn ◽  
...  
Keyword(s):  
Cellulase Activity ◽  
Enzyme Characterization ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Penicillium Decumbens ◽  
Initial Ph ◽  
Effects Of Ph ◽  
Burman Design ◽  
The Stability ◽  
Plackett Burman Design

Plackett-Burman design was employed for screening culture conditions for cellulase production by Penicillium decumbens in submerged fermentation. The results showed that wheat bran was the most significant factor influencing Filter Paper Activity (FPA) of the cellulase, followed by cellulose microcrystalline and initial pH, which could be further optimized for improving the cellulase activity. The effects of pH and temperature on FPA assay were investigated, and optimal FPA could be obtained at pH 4.5 and 60 °C. The stabilities of endo-glucanase (EG), exo-glucanase (CBH) and β-glucosidase (BG) were investigated and compared with that of FPA under different pH and temperature. The results indicated that CBH and FPA were more sensitive to pH and temperature than EG and BG and the stability of CBH was very similar to that of FPA under the conditions.

scholarly journals Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation

2020 ◽  
Vol 9 (7) ◽  
pp. e531974280
Author(s):  
Rafaella Martins de Freitas ◽  
Juliane Döering Gasparin Carvalho ◽  
Laura Maria Bruno ◽  
Gustavo Adolfo Saavedra Pinto
Keyword(s):  
Biomass Production ◽  
Batch Fermentation ◽  
Lactobacillus Rhamnosus ◽  
Ph Control ◽  
Production Scale ◽  
Aeration Conditions ◽  
Ph Conditions ◽  
Mrs Medium ◽  
Mrs Broth

The objective of this work was to produce Lactobacillus rhamnosus BRM 029693 biomass in a fermenter with the aim to expand its production scale. Lactobacillus rhamnosus BRM 029693 was grown in a fermenter under different aeration conditions (in surface and depth) using a commercial substrate (MRS broth) and simplified substrate formulated in the laboratory itself, and under different pH conditions (4.7, 5.2, 5.7). The highest biomass production on a fermenter scale occurred using synthetic MRS, 1 v.v.m. aeration on the medium surface and without pH control (3.2 g/L) among all the tests. The biomass production was only 0.7 g/L when the MRS medium was replaced by simplified MRS. The pH also influenced the biomass production, reaching 0.9 g/L when the fermentation pH was controlled at pH 4.7.

Rapid decolorization of methyl orange by a novel Aeromonas sp. strain DH-6

2014 ◽  
Vol 69 (10) ◽  
pp. 2004-2013 ◽  
Author(s):  
Lin-Na Du ◽  
Gang Li ◽  
Fang-Cheng Xu ◽  
Xiu Pan ◽  
Ling-Ning Wen ◽  
...  
Keyword(s):  
Methyl Orange ◽  
Azo Dye ◽  
Optimal Conditions ◽  
Operational Parameters ◽  
Order Model ◽  
First Order ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Kinetics Of ◽  
Rdna Analysis

Azo dyes are extensively used, but are recalcitrant and refractory. In this study, an indigenous strain DH-6 was isolated and identified as Aeromonas sp. based on 16S rDNA analysis for its excellent methyl orange (MO) decolorizing capability. Plackett–Burman design and response surface methodology (RSM) were employed to investigate the effect of operational parameters on decolorization and to optimize the decolorization process. Based on the results the concentrations of glucose, Na2HPO4 and MO and temperature were selected as the four significant parameters of RSM. The optimal conditions for MO decolorization by the strain were as follows: 3.0 g/L glucose, 4.9 g/L Na2HPO4, 100 mg/L MO, and at 40 °C. The verification tests showed that 95.5% decolorization was observed after incubation for 2 h, which is within the confidence interval. Under the optimal conditions, the kinetics of the decolorization fitted the first-order model well (R2 = 0.969). As the strain DH-6 still showed a good decolorizing capability at a relatively high temperature, it is considered a candidate for azo dye bioremediation in some tropical or subtropical regions.

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