scholarly journals Optimization of Large-Scale Culture Conditions for the Production of Cordycepin withCordyceps militarisby Liquid Static Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Chao Kang ◽  
Ting-Chi Wen ◽  
Ji-Chuan Kang ◽  
Ze-Bing Meng ◽  
Guang-Rong Li ◽  
...  
Keyword(s):  
Large Scale ◽  
Total Content ◽  
Culture Conditions ◽  
Key Factors ◽  
Working Volume ◽  
Large Scale Culture ◽  
Factor Design ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

Cordycepin is one of the most important bioactive compounds produced by species ofCordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.

scholarly journals Optimization of Culture Conditions for Exopolysaccharide Production by a Probiotic Strain of Lactobacillus rhamnosus E/N

2014 ◽  
Vol 63 (2) ◽  
pp. 253-257 ◽  
Author(s):  
MAGDALENA POLAK-BERECKA ◽  
ADAM WAŚKO ◽  
AGNIESZKA KUBIK-KOMAR
Keyword(s):  
Batch Fermentation ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Culture Conditions ◽  
Optimal Conditions ◽  
Working Volume ◽  
Burman Design ◽  
Specific Culture ◽  
Plackett Burman Design ◽  
Mrs Medium

The effects of culture conditions on exopolysaccharides (EPS) production by a probiotic Lb. rhamnosus E/N have been studied using the Plackett-Burman design. Process optimization was performed in stationary cultures to maximize the production of EPS. In order to verify the optimal conditions, an analysis was performed of EPS production in fermentation culture. Batch fermentation was carried out at working volume of 2.51. The optimal temperature, pH, carbon source, and nitrogen source conditions were 37 degrees C, pH 5.0, galactose, and yeast extract, respectively. EPS production was improved by 210.28 mg/l in stationary cultures compared to 134.2 mg/l in a control grown on commercial MRS medium. The fermentor experiment showed the possibility of increasing EPS biosynthesis by 175.8%. Our results clearly demonstrate that in the case of Lb. rhamnosus E/N specific culture conditions can enhance EPS production for possible application in the industry.

scholarly journals Optimization of Extraction or Purification Process of Multiple Components from Natural Products: Entropy Weight Method Combined with Plackett–Burman Design and Central Composite Design

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5572
Author(s):  
Yu Du ◽  
Pengcheng Huang ◽  
Weifeng Jin ◽  
Chang Li ◽  
Jiehong Yang ◽  
...  
Keyword(s):  
Natural Products ◽  
Purification Process ◽  
Entropy Weight ◽  
Multiple Components ◽  
Entropy Weight Method ◽  
Weight Method ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Comprehensive Score

In this paper, the optimization of the extraction/purification process of multiple components was performed by the entropy weight method (EWM) combined with Plackett–Burman design (PBD) and central composite design (CCD). We took the macroporous resin purification of Astragalus saponins as an example to discuss the practicability of this method. Firstly, the weight of each component was given by EWM and the sum of the product between the componential content and its weight was defined as the comprehensive score, which was taken as the evaluation index. Then, the single factor method was adopted for determining the value range of each factor. PBD was applied for screening the significant factors. Important variables were further optimized by CCD to determine the optimal process parameters. After the combination of EWM, PBD and CCD, the resulting optimal purification conditions were as follows: pH value of 6.0, the extraction solvent concentration of 0.15 g/mL, and the ethanol volume fraction of 75%. Under the optimal conditions, the practical comprehensive score of recoveries of saponins was close to the predicted value (n = 3). Therefore, the present study provided a convenient and efficient method for extraction and purification optimization technology of multiple components from natural products.

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

Medium Optimization for the Production of Phytase by Recombinant Pichia pastoris Grown on Glycerol

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Min Liu ◽  
Gabriel Potvin ◽  
Yiru Gan ◽  
Zhanbin Huang ◽  
Zisheng Zhang
Keyword(s):  
Pichia Pastoris ◽  
Phytase Production ◽  
Medium Components ◽  
Gap Promoter ◽  
Recombinant Pichia Pastoris ◽  
Burman Design ◽  
Statistical Designs ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Three Components

Based on statistical designs, minimal salts medium, commonly used for yeast cultivation, was optimized to maximize GAP promoter-mediated phytase production by recombinant Pichia pastoris grown on glycerol. A Plackett-Burman design was followed to screen medium components to determine those that significantly affected phytase production. Of the 8 components studied, the concentrations of K2SO4, CaSO4•2H2O and MgSO4•7H2O were identified as having a significant effect. These three components were subsequently optimized by response surface methodology using a central composite design. The optimal concentrations of the three components, leading to a maximal extracellular phytase activity of 161.64 U/ml, were K2SO4 13.25g/l, CaSO4•2H2O 1.03g/l and MgSO4•7H2O 17.94g/l. The activity measured in cultures using optimized growth medium is significantly higher than the 73.31 U/ml measured in cultures using standard minimal salts media. The theoretical phytase yields predicted by the developed model were very close to experimentally obtained values.

Study on the Key Environmental Factors Aspergillus flavus Growth and Aflatoxin Production

2014 ◽  
Vol 668-669 ◽  
pp. 1550-1553 ◽  
Author(s):  
Chu Shu Zhang ◽  
Qin Zhao ◽  
Jian Xiong Feng ◽  
Jie Sun ◽  
Li Na Yu ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Aspergillus Flavus ◽  
Prevention And Control ◽  
Aflatoxin Production ◽  
Aflatoxin Contamination ◽  
Key Factors ◽  
Culture Time ◽  
Burman Design ◽  
And Control ◽  
Plackett Burman Design

Single factor and Plackett-Burman design were used to determine the key environmental factors for Aspergillus flavus growth and aflatoxin production. The results showed that the key factors were culture time, moisture content. So Controling of moisture was crucial for Aflatoxin contamination prevention and control.

scholarly journals BNC Biosynthesis with Increased Productivity in a Newly Designed Surface Air-Flow Bioreactor

2020 ◽  
Vol 10 (11) ◽  
pp. 3850
Author(s):  
Izabela Cielecka ◽  
Małgorzata Ryngajłło ◽  
Stanisław Bielecki
Keyword(s):  
Mechanical Properties ◽  
Air Flow ◽  
Culture Conditions ◽  
Production Costs ◽  
High Yield ◽  
Flow Ratio ◽  
Culture Time ◽  
Wide Range ◽  
Burman Design ◽  
Plackett Burman Design

The application of bacterial cellulose (BNC) could be widely expanded if the production costs were reduced. This study aims to determine factors simultaneously affecting the yield and tensile strength of BNC in a newly designed surface air-flow bioreactor (SAF). For this purpose, a two-stage study was done. Firstly, the most important factors for high yield were determined based on the Plackett–Burman Design. Secondly, impact of the chosen variables on both responses was assessed in a wide range of factor values. The greatest influence on the yield and mechanical strength was proved for such factors as air-flow ratio, glucose concentration, and culture time. The productivity in a SAF bioreactor with controlled air-flow ratio was enhanced by 65%. In terms of mechanical properties, the stress of BNC membranes varied from 0.8 to 6.39 MPa depending on the culture conditions. The results of the performed tests make a useful basis for future optimizations.

scholarly journals Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp.

2020 ◽  
Vol 32 (6) ◽  
pp. 486-495 ◽  
Author(s):  
Tengku Athirrah Tengku-Mazuki ◽  
Kavilasni Subramaniam ◽  
Nur Nadhirah Zakaria ◽  
Peter Convey ◽  
Khalilah Abdul Khalil ◽  
...  
Keyword(s):  
Central Composite Design ◽  
Phenol Degradation ◽  
Antarctic Bacterium ◽  
Burman Design ◽  
High Concentrations ◽  
Predicted Values ◽  
The Antarctic ◽  
Optimized Conditions ◽  
Plackett Burman Design ◽  
Central Composite

AbstractThis study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l-1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l-1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement (R2 = 0.9785) between experimental and predicted values, with less strong but still good agreement (R2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol.

scholarly journals Determination and Optimization of Flour for Producing Crispy Baby Tilapia using Plackett Burman Design and Central Composite Design Method

2017 ◽  
Vol 12 (1) ◽  
pp. 1
Author(s):  
Syamdidi Syamdidi ◽  
Diah Ikasari ◽  
Hasta Octavini
Keyword(s):  
Central Composite Design ◽  
Oreochromis Niloticus ◽  
Design Method ◽  
Rice Flour ◽  
Corn Flour ◽  
Optimum Amount ◽  
Burman Design ◽  
Tapioca Flour ◽  
Plackett Burman Design ◽  
Central Composite

Research on processing of crispy baby tilapia (Oreochromis niloticus) was conducted to obtain type and proportion of flour on this product with central composite design method. This research used 6 types of flour, namely wheat flour, rice flour, potato flour, tapioca flour, corn flour and baking powder. Baby tilapia used for this research were 30-40 day old, 2-3 cm long. Parameters observed were sensory (appearance, odor, taste, texture, overall acceptance) and crispness for the physical parameter. The results showed that only two out of six variables gave big effect on the tested response i.e potato and rice flour. Those two variables were then optimized with central composite design method to obtain the best product. The optimization process demonstrated that the optimum amount of potato and rice flour were 58-60 g (22.16-22.92%) and 40-60 g (15.28-22.92%), respectively.

scholarly journals Berberine as siderophore from Talaromyces trachyspermus: augmentation and characterization”.

2021 ◽  
Author(s):  
Sharda Sahu ◽  
ANIL Prakash
Keyword(s):  
Response Surface Methodology ◽  
Succinic Acid ◽  
Central Composite Design ◽  
Endophytic Fungus ◽  
Scale Up ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Important Drug ◽  
Significant Factors

In the present study, a siderophore compound produced by an endophytic fungus, Talaromyces trachyspermus was optimized for maximum production, 88.9 % SU by applying Plackett-Burman design and Response Surface Methodology through Central Composite Design that showed the succinic acid (1.141 g/L), sucrose (31.028 g/L) and temperature (27.475 ºC) as significant factors. On scale up, a further increase in siderophore yield was obtained (by 3%) The compound was extracted, purified and detected chemically as catecholate siderophore showing max. λ absorbance at 279nm. Contained of hydroxy benzene as shown by GC-MS analysis and further identified as berberine by HRLC-MS studies. The compound berberine is clinically a very important drug with several ethnobotanical properties. This is rare to report fungal catecholate and first to report the production of berberine from Talaromyces species .In the present study, a siderophore compound produced by an endophytic fungus, Talaromyces trachyspermus was optimized for maximum production, 88.9 % SU by applying Plackett-Burman design and Response Surface Methodology through Central Composite Design that showed the succinic acid (1.141 g/L), sucrose (31.028 g/L) and temperature (27.475 ºC) as significant factors. On scale up, a further increase in siderophore yield was obtained (by 3%) The compound was extracted, purified and detected chemically as catecholate siderophore showing max. λ absorbance at 279nm. Contained of hydroxy benzene as shown by GC-MS analysis and further identified as berberine by HRLC-MS studies. The compound berberine is clinically a very important drug with several ethnobotanical properties. This is rare to report fungal catecholate and first to report the production of berberine from Talaromyces species .

scholarly journals Statistical Optimization by Response Surface Methodology to Enhance Lipase Production by Aspergillus fumigatus

2019 ◽  
Vol 13 (1) ◽  
pp. 86-93 ◽  
Author(s):  
Akshita Mehta ◽  
Roji Sharma ◽  
Reena Gupta
Keyword(s):  
Aspergillus Fumigatus ◽  
Response Surface ◽  
Incubation Time ◽  
Lipase Production ◽  
Inoculum Size ◽  
Coefficient Of Determination ◽  
Burman Design ◽  
Commercial Applications ◽  
Plackett Burman Design ◽  
Central Composite

Background:Lipases have various commercial applications and microorganisms serve as a potential source of production.Objective:The aim of this paper was to study the effect of interactions among different production parameters on lipase yield ofAspergillus fumigatus.Method:Plackett Burman and Central Composite Design (CCD) were established by using Design Expert software 10.0.Results:In the present study, interactions were studied for six different variables such as inoculum size, pH, temperature, galactose concentration, peptone concentration and incubation time. In Plackett-Burman design, galactose concentration, peptone concentration, pH and incubation time were found to be important factors. Using the statistical approach, the optimum factors were found to be as: galactose concentration (1.5%), peptone concentration (1.8%), pH (10.0) and incubation time (72 h) at 45°C under response surface curves. Upon statistical analysis, the coefficient of determination (R2) obtained was 0.9318 which showed that the model was significant.Conclusion:The statistical tools used predicted the optimal conditions for the production of the lipase. The optimized parameters were galactose concentration 1.5%, peptone concentration 1.4%, temperature 45°C, pH 10.0 and incubation time of 72 h for obtaining a maximum lipase activity of 6.22 U/ml.

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