[Duplicate] - Cell adhesion behavior on molecularly engineered surfaces

2010 ◽  
Author(s):  
Ning Cai
Keyword(s):  
Cell Adhesion ◽  
Adhesion Behavior ◽  
Engineered Surfaces

Cell adhesion behavior of poly(ε-caprolactone)/poly( L -lactic acid) nanofibers scaffold

2016 ◽  
Vol 171 ◽  
pp. 178-181 ◽  
Author(s):  
Zeeshan Khatri ◽  
Abdul Wahab Jatoi ◽  
Farooq Ahmed ◽  
Ick-Soo Kim
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Adhesion Behavior

Proliferation and Adhesion of L929 Fibroblasts on Surface with Different Microtopography

2014 ◽  
Vol 1648 ◽  
Author(s):  
Yuta Kurashina ◽  
Shogo Miyata ◽  
Jun Komotori ◽  
Tadayoshi Koyama
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Low Frequency ◽  
Polished Surface ◽  
Stainless Steel Surface ◽  
Cell Counts ◽  
Adhesion Behavior ◽  
Cell Scaffold ◽  
Cell Adhesion And Proliferation ◽  
Number Of Cells

ABSTRACTThree types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.

scholarly journals Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach

2020 ◽  
Vol 21 (5) ◽  
pp. 1749 ◽  
Author(s):  
Thomas J. Bauer ◽  
Erich Gombocz ◽  
Markus Wehland ◽  
Johann Bauer ◽  
Manfred Infanger ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cancer Metastasis ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Binding Activity ◽  
Omics Data ◽  
Adhesion Proteins ◽  
Adhesion Behavior

The adhesion behavior of human tissue cells changes in vitro, when gravity forces affecting these cells are modified. To understand the mechanisms underlying these changes, proteins involved in cell-cell or cell-extracellular matrix adhesion, their expression, accumulation, localization, and posttranslational modification (PTM) regarding changes during exposure to microgravity were investigated. As the sialylation of adhesion proteins is influencing cell adhesion on Earth in vitro and in vivo, we analyzed the sialylation of cell adhesion molecules detected by omics studies on cells, which change their adhesion behavior when exposed to microgravity. Using a knowledge graph created from experimental omics data and semantic searches across several reference databases, we studied the sialylation of adhesion proteins glycosylated at their extracellular domains with regards to its sensitivity to microgravity. This way, experimental omics data networked with the current knowledge about the binding of sialic acids to cell adhesion proteins, its regulation, and interactions in between those proteins provided insights into the mechanisms behind our experimental findings, suggesting that balancing the sialylation against the de-sialylation of the terminal ends of the adhesion proteins’ glycans influences their binding activity. This sheds light on the transition from two- to three-dimensional growth observed in microgravity, mirroring cell migration and cancer metastasis in vivo.

Development of a,b-plane-oriented hydroxyapatite ceramics as models for living bones and their cell adhesion behavior

2013 ◽  
Vol 9 (5) ◽  
pp. 6732-6740 ◽  
Author(s):  
Zhi Zhuang ◽  
Takahiko J. Fujimi ◽  
Mariko Nakamura ◽  
Toshiisa Konishi ◽  
Hideyuki Yoshimura ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Hydroxyapatite Ceramics ◽  
Adhesion Behavior

Effect of Grain Refinement by Laser Surface Treatment on Cell Adhesion Behavior of AZ91D Magnesium Alloy

2019 ◽  
Vol 49 (3) ◽  
pp. 20180779
Author(s):  
Changgang Guo ◽  
Lingqian Wang ◽  
Jiansong Zhou ◽  
Baocheng Cao ◽  
Yimeng Xu ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Magnesium Alloy ◽  
Surface Treatment ◽  
Grain Refinement ◽  
Laser Surface ◽  
Laser Surface Treatment ◽  
Az91d Magnesium Alloy ◽  
Adhesion Behavior

Investigating the role of surface micro/nano structure in cell adhesion behavior of superhydrophobic polypropylene/nanosilica surfaces

2015 ◽  
Vol 127 ◽  
pp. 233-240 ◽  
Author(s):  
Iman Hejazi ◽  
Javad Seyfi ◽  
Ehsan Hejazi ◽  
Gity Mir Mohamad Sadeghi ◽  
Seyed Hassan Jafari ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Nano Structure ◽  
Adhesion Behavior

scholarly journals Influence of Zika Virus in mechanisms related of cytotoxicity, cell adhesion, apoptosis and inflammatory markers on glioblastoma cells

2021 ◽  
Author(s):  
Daniel Marinowic ◽  
Fabiana Spillari Viola ◽  
Fernanda Majolo ◽  
Gabriele Goulart Zanirati ◽  
Pamella Nunes Azevedo ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Adhesion ◽  
Cell Viability ◽  
Cell Line ◽  
Cell Lines ◽  
Zika Virus ◽  
Adhesion Behavior ◽  
Induction Of Apoptosis ◽  
Cd73 Expression ◽  
Potential Use

Abstract Glioblastoma (GBM) is one of the most common brain tumors in adults. Despite the presence of available treatments, it remains one of the most lethal and difficult tumors to treat such that most patients die within two years. Studies reported that infection with Zika virus (ZIKV) causes inhibition of cell proliferation as well as induction of apoptosis; moreover, these manifestations show a predilection for developing neuronal cells. In the present study, two GBM cell lines U-138 and U-251 were infected with ZIKV at multiplicities of infection (MOI) 0.1, 0,01 and 0.001 and tested for cell viability, cell migration, cell adhesion, induction of apoptosis, interleukin levels, and cell surface markers (CD14 and CD73). Our study demonstrated that the ZIKV infection promotes loss of cell viability and increased apoptosis potential. It was not evidenced changes in cell migration, however, the two glioblastoma cell lines displayed increased the cell adhesion behavior. There was small increase in the IL-4 level in the U-251 cell line after exposure to ZIKV, with no change in relation to INF-γ levels. Furthermore, we observed an increase in the percentage of cells expressing the CD14 surface marker in both cell lines and increased CD73 expression in the U-251 cell line. Our results suggest that ZIKV may be associated with decrease of cell viability and increased CD73 expression, enhanced adherence, as well as increased apoptosis rates. Further investigations are required to explore the potential use of ZIKV in the treatment of GBM.

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