Proliferation and Adhesion of L929 Fibroblasts on Surface with Different Microtopography

2014 ◽  
Vol 1648 ◽  
Author(s):  
Yuta Kurashina ◽  
Shogo Miyata ◽  
Jun Komotori ◽  
Tadayoshi Koyama
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Low Frequency ◽  
Polished Surface ◽  
Stainless Steel Surface ◽  
Cell Counts ◽  
Adhesion Behavior ◽  
Cell Scaffold ◽  
Cell Adhesion And Proliferation ◽  
Number Of Cells

ABSTRACTThree types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.

scholarly journals Piperlongumine inhibits migration and proliferation of castration-resistant prostate cancer cells via triggering persistent DNA damage

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ding-fang Zhang ◽  
Zhi-chun Yang ◽  
Jian-qiang Chen ◽  
Xiang-xiang Jin ◽  
Yin-da Qiu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Proliferation ◽  
Dna Damage ◽  
Cell Adhesion ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Castration Resistant Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Dna Damage And Repair ◽  
Castration Resistant

Abstract Background Metastatic castration-resistant prostate cancer (CRPC) is the leading cause of death among men diagnosed with prostate cancer. Piperlongumine (PL) is a novel potential anticancer agent that has been demonstrated to exhibit anticancer efficacy against prostate cancer cells. However, the effects of PL on DNA damage and repair against CRPC have remained unclear. The aim of this study was to further explore the anticancer activity and mechanisms of action of PL against CRPC in terms of DNA damage and repair processes. Methods The effect of PL on CRPC was evaluated by MTT assay, long-term cell proliferation, reactive oxygen species assay, western blot assay, flow cytometry assay (annexin V/PI staining), β-gal staining assay and DAPI staining assay. The capacity of PL to inhibit the invasion and migration of CRPC cells was assessed by scratch-wound assay, cell adhesion assay, transwell assay and immunofluorescence (IF) assay. The effect of PL on DNA damage and repair was determined via IF assay and comet assay. Results The results showed that PL exhibited stronger anticancer activity against CRPC compared to that of taxol, cisplatin (DDP), doxorubicin (Dox), or 5-Fluorouracil (5-FU), with fewer side effects in normal cells. Importantly, PL treatment significantly decreased cell adhesion to the extracellular matrix and inhibited the migration of CRPC cells through affecting the expression and distribution of focal adhesion kinase (FAK), leading to concentration-dependent inhibition of CRPC cell proliferation and concomitantly increased cell death. Moreover, PL treatment triggered persistent DNA damage and provoked strong DNA damage responses in CRPC cells. Conclusion Collectively, our findings demonstrate that PL potently inhibited proliferation, migration, and invasion of CRPC cells and that these potent anticancer effects were potentially achieved via triggering persistent DNA damage in CRPC cells.

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

scholarly journals Effects of an Acetic Acid and Acetone Mixture on the Characteristics and Scaffold–Cell Interaction of Electrospun Polycaprolactone Membranes

2019 ◽  
Vol 9 (20) ◽  
pp. 4350 ◽  
Author(s):  
Minh Hieu Ho ◽  
Thien Bui-Thuan Do ◽  
Nhi Ngoc-Thao Dang ◽  
An Nguyen-My Le ◽  
Hanh Thi-Kieu Ta ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Cell Interaction ◽  
Fibrous Materials ◽  
Cell Adherence ◽  
Physical Strength ◽  
Membrane Morphology ◽  
Cell Scaffold ◽  
Different Types ◽  
Proliferation Assays ◽  
Cell Adhesion And Proliferation

Green electrospinning has attracted great interest since non-toxic solvents were shown to be applicable in the fabrication of fibrous materials while ensuring health safety and environmental protection. Less harmful reagents such as acetone (AC) and acetic acid (AA) have been employed in this field in recent years. However, research in this area is still rare, yielding only preliminary results. In this study, two different types of solvents (pure AC and an AA/AC mixture) were used to fabricate electrospun polycaprolactone (PCL) membranes. Sample morphology, wettability, tensile strength, and chemical composition were compared between two types of membranes. Cell–scaffold interaction was also examined by cell adhesion and proliferation assays. The results demonstrate that the two types of solvents had significant effects on membrane morphology, physical strength, and cell adherence behaviors, which should be considered for different application purposes.

Effect of Surface Potential on NIH3T3 Cell Adhesion and Proliferation

2014 ◽  
Vol 118 (26) ◽  
pp. 14464-14470 ◽  
Author(s):  
Hsun-Yun Chang ◽  
Chih-Chieh Huang ◽  
Kang-Yi Lin ◽  
Wei-Lun Kao ◽  
Hua-Yang Liao ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Surface Potential ◽  
Nih3t3 Cell ◽  
Cell Adhesion And Proliferation ◽  
Effect Of Surface

Cell adhesion behavior of poly(ε-caprolactone)/poly( L -lactic acid) nanofibers scaffold

2016 ◽  
Vol 171 ◽  
pp. 178-181 ◽  
Author(s):  
Zeeshan Khatri ◽  
Abdul Wahab Jatoi ◽  
Farooq Ahmed ◽  
Ick-Soo Kim
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Adhesion Behavior

Effect of extremely low frequency magnetic fields on cell proliferation and gene expression

2015 ◽  
Vol 36 (7) ◽  
pp. 506-516 ◽  
Author(s):  
Hyung Chul Lee ◽  
Mi-Na Hong ◽  
Seung Hee Jung ◽  
Bong Cho Kim ◽  
Young Ju Suh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Magnetic Fields ◽  
Low Frequency ◽  
Extremely Low Frequency
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