scholarly journals Evaluation and Characterization of Microalgae from Kalaburagi Region as a Potential Source of Biomass Production

Author(s):  
Asha Jagan Korban ◽  
A. B. Parameshwar ◽  
M. B. Sulochana

Microalgaes are considered for a large number of applications such as biomass and energy production due to their increased capability of biomass production. Various biotic and abiotic factors influence the growth of microalgae. Among the abiotic factors include the temperature, light intensity, pH of the medium, salinity, and nutritional conditions play the major role. Ten samples of microalgae were collected from different sites of water bodies from Gulbarga University campus. The microalgae isolated were identified and characterized using light microscope, SEM and PCR based 18s rRNA. Influence of cultural conditions such as carbon and nitrogen sources, pH, temperature and salinity on biomass production were studied and analyzed. Among the strains identified, a novel species was isolated and identified as Scenedesmus vacuolatus AK1. The maximum growth rate and biomass productivity can be achieved by providing glucose as carbon source and urea as nitrogen sources at an optimum of 0.5gm/l and 0.1mg/l irrespectively. It was also observed that the strain showed a good growth profile and increase in biomass production at an optimum alkaline pH of 8. Maximum biomass productivity of 0.6g/l was observed at an optimum temperature of 30˚C in BG-11 medium. Maximum biomass yield of 1.45g/l was observed at 5mM NaCl concentration.

2021 ◽  
Vol 13 (4) ◽  
pp. 2216
Author(s):  
Najeeha Mohd Apandi ◽  
Mimi Suliza Muhamad ◽  
Radin Maya Saphira Radin Mohamed ◽  
Norshuhaila Mohamed Sunar ◽  
Adel Al-Gheethi ◽  
...  

The present study aimed to optimize the production of Scenedesmus sp. biomass during the phycoremediation process. The biomass productivity was optimized using face centred central composite design (FCCCD) in response surface methodology (RSM) as a function of two independent variables that included wet market wastewater concentrations (A) with a range of 10% to 75% and aeration rate (B) with a range of 0.02 to 4.0 L/min. The results revealed that the highest biomass productivity (73 mg/L/d) and maximum growth rate (1.19 day−1) was achieved with the 64.26% of (A) and 3.08 L/min of (B). The GC-MS composition analysis of the biomass yield extract revealed that the major compounds are hexadecane (25%), glaucine (16.2%), and phytol (8.33%). The presence of these compounds suggests that WMW has the potential to be used as a production medium for Scenedesmus sp. Biomass, which has several applications in the pharmaceutical and chemical industry.


1983 ◽  
Vol 29 (3) ◽  
pp. 325-330 ◽  
Author(s):  
William A. Ayers ◽  
Peter B. Adams

Additional nutritional and environmental factors affecting growth and sporulation of the beneficial mycoparasite, Sporidesmium sclerotivorum, were determined. A liquid medium composed of mineral salts, glucose, glutamine, thiamine, biotin, and succinic acid supported good growth and sporulation of isolate CS-5 within 5 weeks at 25 °C. Growth was substantially greater in a medium with KH2PO4, CaCl2, minor elements, and ferric–potassium salt of EDTA (FeKEDTA) than in media with single omissions of these ingredients. Iron supplied as FeCl3 or FeKEDTA at 10−5 M or greater markedly stimulated production of conidia. Supplementation of the medium with Casamino acids or with Soytone and yeast extract decreased the lag phase slightly but did not affect the maximum growth rate nor final mycelial and spore yield. Succinic acid (0.2%) buffered the medium against a rapid drop in pH and thereby promoted growth. The growth rate was independent of glucose concentrations of 0.5 to 2%, but the total mycelial yield was dependent upon the total amount of glucose furnished. Conidia developed primarily on mycelial mat surfaces, and maximum spore yields were favored by cultivation in shallow depths of medium.


2017 ◽  
Vol 3 (1) ◽  
pp. 27-33
Author(s):  
RAHAYU WULAN ◽  
ANJA MERYANDINI ◽  
TITI CANDRA SUNARTI

Fermentation of Lactic Acid Bacteria (LAB) Pediococcus pentosaceus can improve the quality of food and its shelf life. Using commercial LAB specific media, de Man Rogosa and Sharpe (MRS) for growth on industrial scale application is not efficient. Tapioca wastewater (TW) still contains some of the nutrients needed for the growth of P. pentosaceus, but needs the enrichment of carbon sources (5% of glucose) and nitrogen sources (ammonium sulfate). This study aimed to use tapioca industrial wastewater with the addition of glucose and ammonium sulfate as an alternative growth media for lactic acid bacteria P. pentosaceus E.1222. The results showed that glucose and nitrogen had no significant effect on the number of bacterial colonies. The highest substrate efficiency was tapioca wastewater (86.81%), MRS broth (53.73%), and TW with 5% of glucose and 1% of ammonium sulfate (43.53%) respectively. Maximum growth rate (μmaks) was found in TW with 5% of glucose and 1% of ammonium sulfate (0,52 hours-1). Increasing the starter volume until 1000 mL in TW with 5% of glucose and 1% of ammonium sulfate showed a slight decrease in the log number of bacteria from 8,836 (50 mL), 8,401 (500 mL), to 8,063 (1000 mL). 


2019 ◽  
Vol 48 (4) ◽  
pp. 1125-1132
Author(s):  
Mehwish Jaffer ◽  
Hammad Ashraf ◽  
Shabnum Shaheen

For optimization of cultural conditions for algal biomass production of two local filamentous freshwater algae, namely Hydrodictyon reticulatum, and Spirogyra communis were cultured. Among all these tested media, H. reticulattum gave maximum biomass (18.6 g/l) in Bristol medium whereas, S. communis gave the biomass of 10.5 g/l in Bristol soil media. Then the effect of different carbon sources (lactose, glucose, cellulose and starch) supplement in Bristol media was evaluated for biomass production. Among all the sources cellulose at 1 g/l was found to be significant for optimal mass production of (15.81 g/l) and (18.6 g/l) of S. communis and H. reticulatum, respectively. Both the algal species gave insignificant results in all other carbon sources. The effect of different nitrogen sources (ammonium nitrate, ammonium sulphate, ammonium chloride, potassium nitrate and urea) was tested for biomass production. Urea at 0.1 g/l was found to be best (20.7 g/1000 ml) for optimal growth of H. reticulatum as compared to (16.86 g/1000 ml) of S. communis. However, cellulose as carbon source and urea as nitrogen source were optimized for significant growth of both the algal species. The comparison between the growth rates of both specimens was evaluated. Both the species gave maximum growth up to 15 days of incubation and then the growth started decreasing gradually. It is indicated that the volumetric growth of H. reticulatum is significant in the selected media as compared to S. communis.


2017 ◽  
Vol 6 (8) ◽  
pp. 5459
Author(s):  
Chandra Teja K. ◽  
Rahman S. J.

Entomopathogenic fungi like Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii are used in biological control of agricultural insect pests. Their specific mode of action makes them an effective alternative to the chemical Insecticides. Virulent strains of Entomopathogenic fungi are effectively formulated and used as bio-insecticides world-wide. Amenable and economical multiplication of a virulent strain in a large scale is important for them to be useful in the field. Culture media plays a major role in the large-scale multiplication of virulent strains of Entomopathogens. Different substrates and media components are being used for this purpose. Yet, each strain differs in its nutritional requirements for the maximum growth and hence it is necessary to standardize the right components and their optimum concentrations in the culture media for a given strain of Entomopathogen. In the current study, three different nitrogen sources and two different carbon sources were tried to standardize the mass multiplication media for seven test isolates of Entomopathogenic fungi. A study was also conducted to determine the ideal grain media for the optimum conidial yields of the test isolates. Yeast extract was found to be the best Nitrogen source for the isolates. The isolates tested, differed in their nutritional requirements and showed variation in the best nitrogen and carbon sources necessary for their growth. Variation was also found in the optimum concentration of both the ingredients for the growth and sporulation of the isolates. In the solid-state fermentation study, rice was found to be the best grain for the growth of most of the fungi followed by barley. The significance of such a study in the development of an effective Myco-insecticide is vital and can be successfully employed in agriculture is discussed.


2020 ◽  
Vol 3 (1) ◽  
pp. 93
Author(s):  
Iulian Constantin Dănilă

Short rotation forestry (SRF) provides an important supply of biomass for investors in this area. In the NE (North-East) part of Romania at the present time are installed over 800 Ha of this kind of crops. The SRF enjoys the support through environmental policies, in relation to climate change and the provisions of the Kyoto Protocol to reduce the concentration of CO2 in the atmosphere. A precise estimate of biomass production is necessary for the sustainable planning of forest resources and for the exchange of energy in ecosystems. The use of the terrestrial laser scanner (TLS) in estimating the production of above ground wood biomass (AGWB) of short rotation forestry (SRF) brings an important technological leap among indirect (non-destructive) methods. TLS technology is justified when destructive methods become difficult to implement, and allometric equations do not provide accurate information. The main purpose of the research is to estimate the biomass productivity on tree parts in short rotation forestry with TLS technology. Measuring the hybrid poplars crops by TLS may have the following consequences: (1) Higher accuracy of the estimate of biomass production in the SRF; (2) cost and time effective measurements over the biomass of tree parts; (3) new and validated allometric equations for SRF in NE Romania; (4) solid instrument for industry to estimate biomass. TLS technology gives accurate estimates for DBH, tree height and location, as much as the volume on segments, commercial volume or crown volume can be determined. The accuracy of these values depends on the original scan data and their co-registration. The research will contribute to the development of knowledge in the field of hybrid crops.


1993 ◽  
Vol 57 (2) ◽  
pp. 332-334 ◽  
Author(s):  
A. Blasco ◽  
E. Gómez

Two synthetic lines of rabbits were used in the experiment. Line V, selected on litter size, and line R, selected on growth rate. Ninety-six animals were randomly collected from 48 litters, taking a male and a female each time. Richards and Gompertz growth curves were fitted. Sexual dimorphism appeared in the line V but not in the R. Values for b and k were similar in all curves. Maximum growth rate took place in weeks 7 to 8. A break due to weaning could be observed in weeks 4 to 5. Although there is a remarkable similarity of the values of all the parameters using data from the first 20 weeks only, the higher standard errors on adult weight would make 30 weeks the preferable time to take data for live-weight growth curves.


1978 ◽  
Vol 14 (1) ◽  
pp. 1-5 ◽  
Author(s):  
J. L. Monteith

SUMMARYFigures for maximum crop growth rates, reviewed by Gifford (1974), suggest that the productivity of C3 and C4 species is almost indistinguishable. However, close inspection of these figures at source and correspondence with several authors revealed a number of errors. When all unreliable figures were discarded, the maximum growth rate for C3 stands fell in the range 34–39 g m−2 d−1 compared with 50–54 g m−2 d−1 for C4 stands. Maximum growth rates averaged over the whole growing season showed a similar difference: 13 g m−2 d−1 for C3 and 22 g m−2 d−1 for C4. These figures correspond to photosynthetic efficiencies of approximately 1·4 and 2·0%.


1968 ◽  
Vol 31 (3) ◽  
pp. 435-448 ◽  
Author(s):  
H. A. Becker ◽  
T. A. Massaro

A study has been made of the varicose instability of an axisymmetrical jet with a velocity distribution radially uniform at the nozzle mouth except for a laminar boundary layer at the wall. The evolutionary phenomena of instability, such as the rolling up of the cylindrical vortex layer into ring vortices, the coalescence of ring vortex pairs, and the eventual disintegration into turbulent eddies, have been investigated as a function of the Reynolds number using smoke photography, stroboscopic observation, and the light-scatter technique.Emphasis has been placed on the wavelength with maximum growth rate. The jet is highly sensitive to sound and the effects of several types of acoustic excitation, including pure tones, have been determined.


2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.


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